Frequency and spectrum of chlorophyll mutations and induced variability in ricebean (Vigna umbellata Thunb, Ohwi and Ohashi)

DOI: 10.18805/lr.v0iOF.10757    | Article Id: LR-3545 | Page : 39-46
Citation :- Frequency and spectrum of chlorophyll mutations and induced variabilityin ricebean (Vigna umbellata Thunb, Ohwi and Ohashi) .Legume Research.2017.(40):39-46

Madhu Patial*, S.R Thakur1, K.P. Singh2 and Anjana Thakur3

Address :

Chaudhary Shrawan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, Himachal Pradesh, India.

Submitted Date : 19-06-2015
Accepted Date : 8-04-2016


A comparative study of frequency and spectrum of chlorophyll mutations induced by physical (gamma rays) and chemical mutagens (EMS) in relation to the effect of mutations in M1 plant, induction of mutants in M2 and variability studies in M3 generation was made in two ricebean (Vigna umbellata Thunb, Ohwi and Ohashi) varieties; one local (Totru Local) and one widely cultivated one (BRS-1) during 2005-2008. The treatments included three doses each of gamma rays (30, 40 and 50 kR) and EMS (0.50, 0.60 and 0.70%). Both gamma rays and EMS proved to be hazardous for germination and plant survival at higher dose and the comparative reduction in these characters was higher under EMS treatments than gamma rays. The frequencies and spectrum of five different kind of induced chlorophyll mutations was in the order; viridis > xantha = albina > chlorina > albo-viridis in BRS-1 and viridis > xantha > albina > chlorina = albo-viridis in Totru Local. EMS was found to be more effective in inducing maximum frequencies of chlorophyll mutants than gamma rays. Highest frequency of chlorophyll mutants were found under O.50% EMS in BRS-1 and 0.70% EMS in Totru Local. Genotypic differences were seen as highest frequency of mutations was observed in Totru Local which was more sensitive to mutagenic treatments than BRS-1. The coefficients of variation for all quantitative characters studied in M3 generation were of higher magnitude compared to control in both the cultivars. The lower doses (30kR in gamma rays and 0.50% in EMS) were found to be the most important doses for inducing desirable variability in ricebean and three traits i.e. pods per cluster, seeds per pod and pod length showed high heritability coupled with high genetic advance in both BRS-1 and Totru Local indicating that breeding for these traits can be achieved by phenotypic selection. 


Chlorophyll mutations EMS Frequency and spectrum Gamma rays Ricebean Variability.


  1. Ambavane, A.R., Sawardekar, S.V., Sawantdesai, S.A. and Gokhale N.B. (2015). Studies on mutagenic effectiveness and efficiency of gamma rays and its effect on quantitative traits in finger millet (Eleusine coracana L. Gaertn). Journal of Radiation Research and Applied Sciences, 8: 120–125.
  2. Brock, R. D. (1965). Induced mutations affecting quantitative characters. Radiation Botany, 5:451-464.
  3. Deepalakshmi, A. J. and Kumar, A.C. R. (2003). Efficiency and effectiveness of physical and chemical mutagens in urdbean (Vigna mungo (L.) Hepper). Madras Agricultural Journal, 90: 485-489. 
  4. Devi, T. R., Prodhan, H. S., Brajendra, N. and Rohinikumar, M. (2002). Effectiveness and efficiency of gamma rays in inducing chlorophyll mutations in M2 generation of three ricebean genotypes (Vigna umbellata (L.) Thunb, Ohwi and Ohashi). Environment and Ecology, 20 : 890-893.
  5. Federer, W.T. (1956) . Augmented (or honnuiaku) designs. Hawaii Plant Research 2 : 191-208. 
  6. Gaul, H. (1964). Mutations in plant breeding. Radiation Botany, 4 :155-232. 
  7. Gustaffson, A. (1954). Mutation, viability and population structure. Acta Agric Scand, 4: 601-31.
  8. Ignacimuthu, S. and Babu, C. R. (1988). Radio sensitivity of the wild and cultivated urd and mungbean. Indian Journal of Genetics and Plant Breeding, 48 : 331-342. 
  9. Karthika, R. and Lakshmi, B. S. (2007). Fixation of lethal dose50 and the performance of M1 population in two soybean varieties. Legume Research, 30 : 49-52. 
  10. Katoch, R. (2013). Nutritional potential of rice bean (Vigna Umbellata): An Underutilized legume. Journal of Food Science, 78: 8-16.
  11. Kham, N. H., Win N. C., Minn, M. (2015). Study on the variability of induced mutation for improvement of local cultivar sorghum (Shweni-15). International Journal of Technical Research and Applications, 3: 139-144.
  12. Kharkwal, M. C. (1998). Induced mutations in chickpea (Cicer arietinum L.) II. Frequency and spectrum of chlorophyll mutations. Indian Journal of Genetics and Plant Breeding, 58 : 465-474.
  13. Konzak, C. F., Nilan, R. A., Wanger, J. and Foster, R. J. (1965). Efficient chemical mutagenesis. Radiation Botany, 5: 49-70. 
  14. Lokesha, R. and Veeresh, L. C. (1993). Induced mutagenesis and genetic improvement of rice bean (Vigna umbellata (Thunb) Ohwi and Ohashi. Legume Research, 16: 37-40. 
  15. Lokesha, R., Vasanth, K. E., Veeresh, L. C. and Shivashankar, G. (1991). Gamma-ray induced genetic divergence in rice bean (Vigna umbellata Thumb Ohwi and Ohashi). In: Golden Jubilee Symposium on Genetic Research and Education: Current trends and the next fifty years, Indian Society of Genetics and Plant Breeding. IARI, New Delhi, p.719.
  16. Mahla, H. R., Kumar, D. and Shekhawat, A. (2010). Effectiveness and efficiency of mutagens and induced variability in clusterbean (Cyamopsis tetragonoloba). The Indian Journal of Agricultural Sciences, 80:1033-7.
  17. Meena, G. S. and Dwivedi, P. (2015). Effect of gamma rays and nitroso methyl urea on seed germination, seedling height and survivability of chickpea (Cicer arietinum L.) var. RSG-963. Trends in Life Sciences, 4: 397-399.
  18. NAS. (1979). Tropical legume : Resources for the future. National Academy of Sciences, Washington DC, pp. 331. 
  19. Novak, F. J. and Brunner, H. (1992). Plant breeding: induced mutation technology for crop improvement. In: IAEA BULLETIN, 4/1992. pp. 25-33.
  20. Prakash, B. G. and Khanure, S. K. (1999). Isolation of mutants and their frequencies under M2 generation in ricebean (Vigna umbellata L. Thunb). Madras Agriculture Journal, 86 : 568-572.
  21. Prakash, B. G. and Shambulingappa, K. G. (1999). Estimation of chlorophyll and viable mutation under M2 generation in ricebean (Vigna umbellata L. Thunb). Karnataka Journal of Agriculture Sciences, 12 : 38-42.
  22. Sangsiri, C., Sarajjapinun, W. and Srinives, P. (2005). Gamma radiation induced mutations in mungbean. Science Asia, 31 : 251-255. 
  23. Sharma, J. R. (1998). Statistical and Biometrical Techniques in Plant Breeding. New Age International (P) Limited, p. 429.
  24. Sharma, S.K. (1990). Mutagenic effectiveness and efficiency in microsperma lentil. Cytologia, 55:243-247.
  25. Shu, Q. Y. (2009). Induced plant mutations in the genomics era. In: IAEA. Food and Agriculture Organization of United Nationals, Rome, pp. 455-458.
  26. Singh, B. and Rao, G. T. (2007). Induced chlorophyll mutations in green gram (Vigna radiata (L.) Wilczek). Legume Research, 30 : 308-310. 
  27. Singh, S. P., Singh, R. P., Singh, N. K., Prasad, J. P. and Sahi, J. P. (2007). Mutagenic efficiency of gamma rays, EMS and its combination on microsperma lentil. International Journal of Agricultural Sciences, 3: 113-118. 
  28. Sinha, A. and Lal, J. P. (2007). Effect of mutagens on M1 parameters and qualitative changes induced in M2 generation in lentil. Legume Research, 30 : 180-185. 
  29. Swaminathan, M.S., Chopra, V.L. and Bhaskaran, S. (1962). Chromosome aberrations, the frequency and spectrum of mutations induced by EMS in barley and wheat. Indian Journal of Genetics and Plant Breeding, 22: 190-207
  30. Waghmare, V. N. and Mehra, R. B. (2001). Induced chlorophyll mutations, mutagenic effectiveness and efficiency in Lathyrus sativus L. Indian Journal of Genetics and Plant Breeding, 61: 53-56.

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