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Temperature induction response reveals intrinsic thermotolerant genotypes in soybean

DOI: 10.18805/lr.v0iOF.9595    | Article Id: LR-3478 | Page : 926-930
Citation :- Temperature induction response reveals intrinsicthermotolerant genotypes in soybean .Legume Research.2016.(39):926-930

Uwimana Marie Ange, S. Srividhya, C. Vijayalakshmi and P. Boominathan*

boominathan.p@tnau.ac.in
Address :

Department of Crop Physiology, Tamil Nadu  Agricultural University, Coimbatore-341 003, India.

Submitted Date : 2-03-2015
Accepted Date : 11-03-2016

Abstract

The responses of soybean genotypes to high temperature for intrinsic tolerance was studied using Temperature Induction Response (TIR) technique in order identify the genotypes tolerant to high temperature stress. Seven soybean genotypes subjected to lethal and sub lethal temperatures showed significant variation for acquired thermotolerance. Thermotolerant genotypes ADT 1 and CoSoy1 identified by the TIR technique, demonstrated higher survival percentage, and lower growth reduction.  Further the tolerant genotypes identified based on TIR also showed higher antioxidant enzymes activity implying the critical role of antioxidant in cellular thermotolerance. This clearly demonstrated that TIR can be effectively used for screening high temperature tolerance genotypes in soybean. This study also established the fact that the alternations in antioxidants during induction are vital for imparting tolerance to high temperature stress.  

Keywords

Acquired thermotolerance Antioxidant enzymes Sub lethal temperature.

References

  1. Aebi, H. (1974). Catalases. In: [Bergmeyer HU (ed)] Methods of Enzymatic Analysis. 2. Academic Press. NY, pp 673-684.
  2. Angelini, R.F. Manes and Federico, R. (1990). Spatial and functional correlation between diamineoxidase and peroxidase activities and their dependence upon etiolation and wounding in chickpea stems. Planta. 182: 89-96.
  3. Babu, S., A. Sheeba, P. Yogameenakshi, J. Anbumalarmathi and P. Rangasamy. (2007). Effect of salts stress in the selection of salt tolerant hybrids in (Oryza sativa L.) under in vitro and in vivo condition. Asian J. Plant Sci. 6: 137-142.
  4. Cao, Y.Y., H. Duan, L.N. Yang, Z.Q. Wang, L.J. Liu and J.C. Yang. (2009). Effect of high temperature during heading and early filling on grain yield and physiological characteristics in Indica rice. Acta Agron. Sin. 35: 512-521.
  5. Dornbos, D.L. and Mullen, R.E. (1991). Influence of stress during soybean seed fill on seed weight, germination, and seedling growth rate. J. Plant Sci. 71: 373-383.
  6. Fadzillah, N.M., V. Gill, R.P. Finch and R.H. Burdon. (1996). Chilling, oxidative stress and antioxidant responses in shoot cultures of rice. Planta 199: 552-556.
  7. Gangappa, E., K. Ravi and G.N. Veera Kumar. (2006). Evaluation of groundnut (Arachis hypogaea L.) genotypes for temperature tolerance based on temperature Induction Response (TIR) technique. Indian J. Genet. 66:127-130.
  8. Gomez, K.A. and Gomez, A.A. (1984). Statistical Procedure for Agricultural Research. An IRRI book, Wiley Inter Science Publication, John Wile and Sons, New York, USA.P.680.
  9. Gulen, H. and Eris, A. (2004). Effect of heat stress on peroxidase activity and total protein content in strawberry plants. Plant Sci. 166: 739-744.
  10. Joseph, C.N., E.H. James and H.H. Richard. (1976). Nitrate Reductase Activity in Soybeans (Glycine max). Plant Physiol. 58: 731-735.
  11. Kumar, G., B.T. Krishnaprasad, M. Savitha, R. Gopalakrishna, K. Mukkhopadhyay, G. Rammohan and M. Udayakumar. (1999). Enhanced expression of heat shock proteins in thermotolerant lines from the progenies selected on the basis of temperature induction response (TIR). Theor. Appl. Genet. 99: 359–367.
  12. Kumar, R.R., K. Karajol and G.R. Naik. (2011). Effect of Polyethylene Glycol Induced Water Stress on Physiological and Biochemical Responses in Pigeon pea. Rec. Res. Sci. Tech. 3: 148-152.
  13. Larkindale, J., M. Mishkind , E. Vierling. (2005). Plant responses to high temperature. In: [M, Jenks, P. Hasegawa, (eds)], Plant Abiotic Stress. Blackwell, Oxford.
  14. Lobell, D.B. and G.P. Asner. (2003). Climate and management contributions to recent trends in U.S. agricultural yields. Sci. 299:1032.
  15. Mohammadi, D.H., M. Rohami and S. Mafakheri. (2011). Effect of drought stress on antioxidant enzymes activity in some chickpea cultivars. Am. Eurasian J. Agric. Environ. Sci. 11: 782-785.
  16. Peru, N.G. (1962). Measurement of peroxidase activity in plant tissues. Curr. Sci. 31: 71-81.
  17. Sapna, H. (2011). Physiological evaluation of rice genotypes for high temperature stress. MSc. Thesis submitted to Tamilnadu agricultural university. Coimbatore.
  18. Sapna Harihar, S. Srividhya, C.Vijayalakshmi and P.Boominathan.(2014). Temperature induction response technique - A physiological approach to identify thermotolerant genotypes in rice. Internat.J. agric. Sci.,10:230-232
  19. Senthil Kumar, M. (2001). Development and Characterization of thermotolerant sunflower (Helianthus annus L.) hybrid: an approach based on temperature induction response (TIR) and molecular analysis. M.Sc. Thesis submitted to University of Agricultural Sciences, Bangalore-65.
  20. Senthil Kumar, M., V. Srikantbabu, B. Mohan Raju , Ganesh Kumar, Shiva Prakash and M. Udayakumar. 2003. Screening of inbred lines to develop thermotolerant sunflower hybrids through temperature induction response (TIR). A novel approach by exploiting residential variability. J. Exp. Bot., 54: 2569–78.
  21. Senthil Kumar, M., G. Kumar, V. Srikanthbabu and M. Udayakumar. (2006). Assessment of variability in acquired tolerance: Potential option to study genotypic responses and the relevance of stress genes. J. Plant Physiol. 164:111–125.
  22. Sionit, N., B.R. Strain and E.P. Flint. (1987). Interaction of temperature and CO2 enrichment on soybean: Growth and dry matter partitioning. Can. J. Plant Sci. 67: 59–67.
  23. Srikanth Babu, V. (1999). Identification of high temperature stress tolerant genotypes in Garden pea (Pisum sativum L. Hortense) using Temperature Induction Response (TIR) technique. M.Sc. Thesis submitted to Department of Crop Physiology, University of Agricultural Sciences, Bangalore-65.
  24. Srikanthbabu, V., Ganesh-kumar, Bendehokkalu, T. Krishnaprasad, R. Gopalakrishna, M. Savitha and M. Udayakumar. (2001). Identification of pea genotypes with enhanced thermotolerance using temperature induction response technique (TIR). J. Plant Physiol. 159: 535–545.
  25. Thuzar, M., A.B. Puteh, N.A.P. Abdullah, M.B. Mohd. Lassim, K. Jusoff. (2010). The effects of temperature stress on the quality and yield of soybean (Glycine max L.) J. Agri. Sci. 2.
     

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