Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.391

  • Impact Factor 0.8 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Legume Research, volume 39 issue 5 (october 2016) : 704-708

Genetic diversity of selected accessions for seed protein among pigeonpea minicore collection

L.G. Jaggal, B.R. Patil*, P.M. Naik, K. Priya
1<p>Department of Genetics and Plant Breeding,&nbsp;University of Agricultural Sciences, Dharwad-580005, Karnataka, India.</p>
Cite article:- Jaggal L.G., Patil* B.R., Naik P.M., Priya K. (2016). Genetic diversity of selected accessions for seed protein amongpigeonpea minicore collection . Legume Research. 39(5): 704-708. doi: 10.18805/lr.v0iOF.9486.

Protein is an important part of our diet and legumes viz., pigeonpea, chickpea, groundnut, cowpea, blackgram, greengram, etc are very rich source of protein. Among them, pigeonpea consists of about 20 to 22% of seed protein. Minicore collection set is consisted of approximately 10 % of core collection and 1% of entire collection with lot of diversity present in it. Nitrogen content of pigeonpea minicore collection set was estimated using Micro-Kjeldhal method and was ranged from 9.82 to 21.45 %. Based on per cent seed protein, twelve accessions of high and twelve accessions of low seed protein were selected for molecular characterization using 23 simple sequence repeat (SSR) markers. The PowerMarker result depicted that, among twenty three amplified markers, twenty markers generated polymorphism with mean polymorphic information content (PIC), major allele frequency (MAF) and genetic diversity of 0.479, 0.589 and 0.525 respectively. Based on molecular characterization, the dendrogram was constructed using DARwin 5.0 software distinguished the selected accessions of both high and low seed protein separately into different clusters showing diversity The results illustrate the potential of marker systems to distinguish the content of seed protein in pulses crop at genus level. Use of these markers also offers an efficient system for the assessment of genetic diversity within minicore set of pigeonpea.


  1. Burns, M. J., Edwards, K. J., Newbury, H. J., Ford-Lloyd, B. V and Baggott, C. D. (2001) Development of Simple Sequence Repeat (SSR) markers for the assessment of gene flow and genetic diversity in pigeonpea (Cajanus cajan.) Mol. Ecol. Notes., 1: 283-285.

  2. Chitra, U., Singh, U and Rao, V. P. (1996). Phytic acid, in vitro protein digestibility, dietary fiber and minerals of pulses as influenced by processing methods. Plant. Food Hum. Nut., 49: 307–316.

  3. Datta, J., Nand, L., Mayank, K., Prasoon, P and Gupta. (2010). Efficiency of Three PCR based Marker Systems for Detecting DNA Polymorphism in Cicer arietinum L and Cajanus cajan L Millspaugh. Genet. Eng. Biotechnol. J., 25: 58-62. 

  4. Doldi, M. L., Vollmann, J and Lelley, T. (1997). Genetic diversity in soybean as determined by RAPD and microsatellite analysis. Plant Breeding., 116: 331–335.

  5. Edwards, K. J., Barker, J. H. A., Daly, A., Jones, C. and Karp, A. (1996). Microsatellite libraries enriched for several microsatellite sequences in plants. Biotechniques., 20: 758–759.

  6. FAO (1982) Legumes in human nutrition. Food and Agriculture Organization of the United Nations. Food and Nutrition Series No. 20 Rome.

  7. Graham, P. H and Vance, C. P (2003). Legumes- importance and constraints to greater use. Pl. Physiology., 131: 872–877.

  8. Jaggal, L. G (2012). Molecular characterization of pigeonpea (Cajanus cajan (L.) Millsp) accessions to sterility mosaic virus and fusarium wilt. M. Sc (Agri) Thesis. University of Agricultural Sciences, Dharwad, Karnataka.

  9. Kumar Rao, J. V. D. K., Dart, P. J and Santry, P. V. S. S. (1983). Residual effect of pigeonpea [Cajanus cajan] on yield and nitrogen response of maize. Exptl. Agriculture. 19:131-134.

  10. Liu, K and Muse, S. V. (2005). Power marker: Integrated analysis environment for genetic marker data. Bioinform., 21: 2128-2129.

  11. Odeny, A., Jayashree, B., Ferguson, M., Hoisington, D., Crouch, J and Gebhardt, C. (2007). Development, characterization and utilization of microsatellite markers in Pigeonpea. Plant Breeding., 126: 130–136.

  12. Odeny, A., Jayashree, B., Gebhardt, C and Crouch, J. (2009). New microsatellite markers for pigeonpea [Cajanus cajan (L.) millsp.]. BMC Research Notes, 2: 35.

  13. Perrier, X., Flori, A and Bonnot, F (2003). Data analysis methods. Science Publishers Montpellier, 43-76.

  14. Sanghai-Maroof, M. A., Soliman, K. M., Jorgenson, R and Allard, R. W (1984). Ribosomal DNA spacer length polymorphism in barley: Mendalian inheritance, chromosomal locations and population dynamics. Proc. Natl. Acad. Sci. USA, 81: 8014-8018.

  15. Saxena, K. B., Faris, D. G and Kumar, R. V (1987). Relationship between seed size and protein content in newly developed high protein lines of pigeonpea. Plant. Foods. Hum. Nut.,, 36:335–340.

  16. Saxena, K. B., Kumar, R. V and Rao, P. V (2002) Pigeonpea nutrition and its improvement. In: Quality improvement in crops. Crop. Sci., USA pp. 227-260.

  17. Saxena, R. K., Saxena, K and Varshney, R. K (2010a). Application of SSR markers for molecular characterization of hybrid parents and purity assessment of ICPH 2438 hybrid of pigeonpea [Cajanus cajan (L.) Millspaugh]. Mol. Breed., 26:371–380.

  18. Saxena, R. K., Saxena, K. B., Kumar, R. V., Hosington, D. A and Varshney, R. K (2010b). Simple sequence repeat-based diversity in elite Pigeonpea genotypes for developing mapping populations to map resistance to FW and SMD. Plant. Breeding., 129: 135-141.

  19. Upadhyaya H. D., Reddy L. J., Gowda C. L. L., Reddy K. N. And Sube Singh, (2006). Development of minicore subset for enhanced and diversified utilization of pigeonpea germplasm resources. Crop Science. 46: 2127- 2132.

  20. Vadiveli, V and Janardhanan, K (2005). Nutritional and antinutritional characteristics of seven south Indian wild legumes. Plant. Food Hum. Nut., 60: 69-75.

  21. Vavilov, N. I. (2001). The origin, variation, immunity and breeding of cultivated plants. Translated by Start K. Chron. Bot. 13: 1-366.

  22. Yang, S. Y., Pang, W., Ash, G., Harper, J., Carling, J., Wenzl, P., Huttner, E., Zong, X and Kilian, A (2006). Low level of genetic diversity in cultivated pigeonpea compared to its wild relatives is revealed by diversity arrays technology. Theor. Appl. Genet., 113: 585–595.

     

Editorial Board

View all (0)