Genetic diversity was assessed in 50 accessions of seven Lens species using ISSR markers. The collection included accessions of the cultivated species L. culinaris and six wild species, viz., L. culinaris ssp. odemensis, L. culinaris ssp. orientalis, L. orientalis, L. nigricans, L. lamottei and L. ervoides. The 23 ISSR primers amplified a total of 368 bands with an average of 16 bands per primer. Maximum number of 20 bands was amplified using each of the primers ISSR-34 and ISSR-835. All the primers were found to be polymorphic. PIC values ranged from 0.02 to 0.80. The primers ISSR- 807, ISSR- 809, ISSR- 827, ISSR- 847, ISSR- 28 and ISSR- 37 were found to be very useful for analyzing the molecular diversity of the genus Lens. Cluster Analysis and Principal Coordinates Analyses placed the 50 accessions into two groups and complemented each other.