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Research Article

volume 42 issue 1 (february 2019) : 66-71,   Doi: 10.18805/LR-338

Variations in osmo-regulatory and anti-oxidative status and their links with total nitrogen and phosphorus concentrations in lucerne during re-growth

Z
Zhennan Wang
M
Mei Yang
J
Jiaoyun Lu
H
Huimin Yang
1College of Agriculture and Forestry Science, Linyi University, Linyi 276000, China

  • Submitted23-12-2016|

  • Accepted24-02-2017|

  • First Online 18-09-2017|

  • doi 10.18805/LR-338

Cite article:- Wang Zhennan, Yang Mei, Lu Jiaoyun, Yang Huimin (2017). Variations in osmo-regulatory and anti-oxidative status and their links with total nitrogen and phosphorus concentrations in lucerne during re-growth. Legume Research. 42(1): 66-71. doi: 10.18805/LR-338.

ABSTRACT

This study aimed to find out the changes in osmo-regulatory and anti-oxidative status during lucerne re-growth, elucidating their possible links with total nitrogen (N) and phosphorus (P) concentrations. The concentrations of osmo-regulatory substances, the activities of anti-oxidative enzymes and malondialdehyde concentration tended to increase after mowing. Total N and P concentrations decreased from 13 to 18 d after mowing. All responses were organ-specific. There were negative correlations of total N and P concentrations with osmo-regulatory and anti-oxidative status mostly in stems.

REFERENCES

  1. Agrell, J., McDonald, E.P., and Lindroth, R.L. (1999) Responses to defoliation in deciduous trees: Effects of CO2 and light. Ecol Bullet 47:84-95.
  2. Amor, N.B., Hamed, K.B., Debez, A., and Abdelly, C. (2005) Physiological and antioxidant responses of the perennial halophyte Crithmum maritimum to salinity. Plant Sci 168:889-899.
  3. Asseng, S., and Hsiao, T.C. (2000) Canopy CO2 assimilation, energy balance, and water use efficiency of an alfalfa crop before and after cutting. Field Crop Res 67:191-206.
  4. Bates, L.S., Waldren, R.P., and Teare, I.D. (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205-207.
  5. Bennett, S.M. (2012) Dry matter production of lucerne (Medicago sativa L.) under rotational grazing at Ashley Dene. pp. 1-73. Lincoln University, New Zealand.
  6. Castillo, F.J., Penel, C., and Greppin, H. (1984) Peroxidase release induced by ozone in Sedum album leaves. Plant Physiol 74:846-851.
  7. Cavalcanti, F.R., Oliveira, J.T.A., Martins-Miranda, A.S., Viégas, R.A., and Silveira, J.A.G. (2004) Superoxide dismutase, catalase and peroxidase activities do not confer protection against oxidative damage in salt-stressed cowpea leaves. New Phytol 163:563-571.
  8. Fahanestock, J.T., and Detling, J.K. (2000) Morphological and physiological responses of perennial grasses to long-term grazing in the Pryor mountains, Montana. Am Midl Nat 143: 312-320.
  9. Georgiou, C.D., Grintzalis, K., Zervoudakis, G., and Papapostolou, I. (2008) Mechanism of coomassie brilliant blue G-250 binding to proteins: a hydrophobic assay for nanogram quantities of proteins. Anal Bioanal Chem 391:391-403.
  10. He, S.B., Liu, G.L., and Yang, H.M. (2009) Changes and mechanism in response of photosynthetic rates of lucerne residue to cutting under different water treatments. Acta Prat Sin 18: 192-197.
  11. He, S.B., Liu, G.L., and Yang, H.M. (2012) Water use efficiency by alfalfa: mechanism involving anti-oxidation and osmotic adjustment under drought. Russ J Plant Physl 59:348-355.
  12. Heichel, G.H., and Turner, N.C. (1984) Branch growth and leaf numbers of red maple (Acer rubrum L.) and red oak (Quercus rubra L.): response to defoliation. Oecologia 62:1-6.
  13. Hou, F. (2001) Effect of grazing on photosynthesis and respiration of herbage and on its absorption and transporation of nitrogen and carbon. Chinese J Appl Ecol 12:938-942.
  14. Imaran, M., and Gurmani, Z.A. (2011) Role of macro and micro nutrients in the plant growth and development. Sci Tech Dev 30:36-40.
  15. Kenten, R.H., and Mann, P.J.G. (1952) Hydrogen peroxide formation in oxidations catalysed by plant a-hydroxyacid oxidase. Biochem J 52:130-134.
  16. Lu, J., He, S.,Wang, Z., and Yang, H. (2016) Carbon, nitrogen and phosphorus stoichiometry in differently aged lucerne stands during flowering. Legume Res 39:595-600.
  17. Markoviæ, J., Štrbanoviæ, R., Cvetkoviæ, M., Anðelkoviæ, B., and Živkoviæ, B. (2009) Effects of growth stage on the mineral concentrations in alfalfa(Medicago sativa L.) Leaf, stem and the whole plant. Biotech Anim Husb 25:1225-1231.
  18. Martiniello, P., Paoletti, R., and Berardo, N. (1997) Effect of phonological stages on dry matter and quality components in lucerne. Eur J Agron 6:79-87.
  19. Meuriot, F., Avice, J.C., Simon, J.C., Laine, P., Decau, M.L., and Ourryy, A. (2004) Influence of initial organic N reserves and residual leaf area on growth, N uptake, N pratitioning and N storage in alfalfa (Medicago sativa) during post-cutting regrowth. Ann Bot-London 94:311-321.
  20. Orodho, A.B., and Trlica, M.J. (1990) Clipping and long-term grazing effects on biomass and carbohydrate reserves of Indian ricegrass. J Range Manage 43:52-57.
  21. Ourry, A., Kim, T.H.,and Boucaud, J. (1994) Nitrogen reserve mobilization during regrowth of Medicago sativa L. - Relationships between availability and regrowth yield. Plant Physiol 105: 831-837.
  22. Prud’homme, M.P., Gonzalez, B., Billard, J.P., Boucaud, J. (1992) Carbohydrate content, fructan and sucrose enzyme activities in roots, stubble and leaves of ryegrass (Lolium perenne L.) as affected by source/sink modification after cutting. J Plant Physiol 140: 282-291.
  23. Puckette, M.C., Weng, H., and Mahalingam, R. (2007) Physological and biochemical responses to acute ozone-induced oxidative stress in Medicago truncatula. Plant Physiol 144:1104-1114.
  24. Richards, J.H., and Calldwell, M.M. (1985) Soluble carbohydrates, concurrent photosynthesis and efficiency in regrowth following defoliation: A field study with Agropyron species. J Appl Ecol 22:907-920.
  25. Rong, Y., Han, J., Wang, P., and Mao, P. (2000) Effects of cutting rate on carbohydrate reserves and nitrogen content of russian wildryegrass. Grassl China 2:28-34.
  26. Sánchez, F.J., Manzanares, M., Andres, E.F., Tenorio, J.L., and Ayerbe, L. (1998) Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. Field Crop Res 59: 225-235.
  27. Stewart, R.R.C., and Bewley, J.D. (1980) Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiol 65:245-248.
  28. Thomson, V.P., Cunningham,S.A., Ball, M.C., and Nicotra, A.B. (2003) Compensation for herbivory by Cucumis sativus through increased photosynthetic capacity and efficiency. Oecologia 134: 167-175.
  29. Usha, T.N., and Dadlani, M. (2016) Study of free radical and peroxide scavenging enzymes and content in different vigour lots of soybean (Glycine max). Legume Res 39:233-236.
  30. von-Caemmerer, S., and Farquhar, G.D. (1984). Effects of partial defoliation, changes of irradiance during growth, short-term water stress and growth at enhanced P(CO2) on the photosynthetic capacity of leaves of Phaseolus vulgaris L. Planta 160:320-329.
  31. Wang, J., Yang, C., Han, W., and Liu, M. (2003) Effects on water-soluble carbohydrate of Artemisia frigida under different defoliation inensities. Acta Ecol Sin 23:908-913.
  32. Wright, I.J., and Westoby, M. (2003) Nutrient concentration, resorption and lifespan: leaf traits of Australian sclerophyll species. Funct Ecol 17:10-19.
  33. Yang, H.M., Wang, Z.N., and Ji, C.R. (2013) Research progress in the dynamics of carbon and nitrogen in forages after cutting and grazing. Chin J Grassl 35:102-109.
  34. Zhang, D., Lei, J., Hong, E., Lu, D., Yuan, W., Yang, Z., and Ming, C. (2016) Anti-hypoxia effects of the ethanol extract of Oxytrois ochracephala. Legume Res 39:914-920.
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    Research Article

    volume 42 issue 1 (february 2019) : 66-71,   Doi: 10.18805/LR-338

    Variations in osmo-regulatory and anti-oxidative status and their links with total nitrogen and phosphorus concentrations in lucerne during re-growth

    Z
    Zhennan Wang
    M
    Mei Yang
    J
    Jiaoyun Lu
    H
    Huimin Yang
    1College of Agriculture and Forestry Science, Linyi University, Linyi 276000, China

    • Submitted23-12-2016|

    • Accepted24-02-2017|

    • First Online 18-09-2017|

    • doi 10.18805/LR-338

    Cite article:- Wang Zhennan, Yang Mei, Lu Jiaoyun, Yang Huimin (2017). Variations in osmo-regulatory and anti-oxidative status and their links with total nitrogen and phosphorus concentrations in lucerne during re-growth. Legume Research. 42(1): 66-71. doi: 10.18805/LR-338.

    ABSTRACT

    This study aimed to find out the changes in osmo-regulatory and anti-oxidative status during lucerne re-growth, elucidating their possible links with total nitrogen (N) and phosphorus (P) concentrations. The concentrations of osmo-regulatory substances, the activities of anti-oxidative enzymes and malondialdehyde concentration tended to increase after mowing. Total N and P concentrations decreased from 13 to 18 d after mowing. All responses were organ-specific. There were negative correlations of total N and P concentrations with osmo-regulatory and anti-oxidative status mostly in stems.

    REFERENCES

    1. Agrell, J., McDonald, E.P., and Lindroth, R.L. (1999) Responses to defoliation in deciduous trees: Effects of CO2 and light. Ecol Bullet 47:84-95.
    2. Amor, N.B., Hamed, K.B., Debez, A., and Abdelly, C. (2005) Physiological and antioxidant responses of the perennial halophyte Crithmum maritimum to salinity. Plant Sci 168:889-899.
    3. Asseng, S., and Hsiao, T.C. (2000) Canopy CO2 assimilation, energy balance, and water use efficiency of an alfalfa crop before and after cutting. Field Crop Res 67:191-206.
    4. Bates, L.S., Waldren, R.P., and Teare, I.D. (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205-207.
    5. Bennett, S.M. (2012) Dry matter production of lucerne (Medicago sativa L.) under rotational grazing at Ashley Dene. pp. 1-73. Lincoln University, New Zealand.
    6. Castillo, F.J., Penel, C., and Greppin, H. (1984) Peroxidase release induced by ozone in Sedum album leaves. Plant Physiol 74:846-851.
    7. Cavalcanti, F.R., Oliveira, J.T.A., Martins-Miranda, A.S., Viégas, R.A., and Silveira, J.A.G. (2004) Superoxide dismutase, catalase and peroxidase activities do not confer protection against oxidative damage in salt-stressed cowpea leaves. New Phytol 163:563-571.
    8. Fahanestock, J.T., and Detling, J.K. (2000) Morphological and physiological responses of perennial grasses to long-term grazing in the Pryor mountains, Montana. Am Midl Nat 143: 312-320.
    9. Georgiou, C.D., Grintzalis, K., Zervoudakis, G., and Papapostolou, I. (2008) Mechanism of coomassie brilliant blue G-250 binding to proteins: a hydrophobic assay for nanogram quantities of proteins. Anal Bioanal Chem 391:391-403.
    10. He, S.B., Liu, G.L., and Yang, H.M. (2009) Changes and mechanism in response of photosynthetic rates of lucerne residue to cutting under different water treatments. Acta Prat Sin 18: 192-197.
    11. He, S.B., Liu, G.L., and Yang, H.M. (2012) Water use efficiency by alfalfa: mechanism involving anti-oxidation and osmotic adjustment under drought. Russ J Plant Physl 59:348-355.
    12. Heichel, G.H., and Turner, N.C. (1984) Branch growth and leaf numbers of red maple (Acer rubrum L.) and red oak (Quercus rubra L.): response to defoliation. Oecologia 62:1-6.
    13. Hou, F. (2001) Effect of grazing on photosynthesis and respiration of herbage and on its absorption and transporation of nitrogen and carbon. Chinese J Appl Ecol 12:938-942.
    14. Imaran, M., and Gurmani, Z.A. (2011) Role of macro and micro nutrients in the plant growth and development. Sci Tech Dev 30:36-40.
    15. Kenten, R.H., and Mann, P.J.G. (1952) Hydrogen peroxide formation in oxidations catalysed by plant a-hydroxyacid oxidase. Biochem J 52:130-134.
    16. Lu, J., He, S.,Wang, Z., and Yang, H. (2016) Carbon, nitrogen and phosphorus stoichiometry in differently aged lucerne stands during flowering. Legume Res 39:595-600.
    17. Markoviæ, J., Štrbanoviæ, R., Cvetkoviæ, M., Anðelkoviæ, B., and Živkoviæ, B. (2009) Effects of growth stage on the mineral concentrations in alfalfa(Medicago sativa L.) Leaf, stem and the whole plant. Biotech Anim Husb 25:1225-1231.
    18. Martiniello, P., Paoletti, R., and Berardo, N. (1997) Effect of phonological stages on dry matter and quality components in lucerne. Eur J Agron 6:79-87.
    19. Meuriot, F., Avice, J.C., Simon, J.C., Laine, P., Decau, M.L., and Ourryy, A. (2004) Influence of initial organic N reserves and residual leaf area on growth, N uptake, N pratitioning and N storage in alfalfa (Medicago sativa) during post-cutting regrowth. Ann Bot-London 94:311-321.
    20. Orodho, A.B., and Trlica, M.J. (1990) Clipping and long-term grazing effects on biomass and carbohydrate reserves of Indian ricegrass. J Range Manage 43:52-57.
    21. Ourry, A., Kim, T.H.,and Boucaud, J. (1994) Nitrogen reserve mobilization during regrowth of Medicago sativa L. - Relationships between availability and regrowth yield. Plant Physiol 105: 831-837.
    22. Prud’homme, M.P., Gonzalez, B., Billard, J.P., Boucaud, J. (1992) Carbohydrate content, fructan and sucrose enzyme activities in roots, stubble and leaves of ryegrass (Lolium perenne L.) as affected by source/sink modification after cutting. J Plant Physiol 140: 282-291.
    23. Puckette, M.C., Weng, H., and Mahalingam, R. (2007) Physological and biochemical responses to acute ozone-induced oxidative stress in Medicago truncatula. Plant Physiol 144:1104-1114.
    24. Richards, J.H., and Calldwell, M.M. (1985) Soluble carbohydrates, concurrent photosynthesis and efficiency in regrowth following defoliation: A field study with Agropyron species. J Appl Ecol 22:907-920.
    25. Rong, Y., Han, J., Wang, P., and Mao, P. (2000) Effects of cutting rate on carbohydrate reserves and nitrogen content of russian wildryegrass. Grassl China 2:28-34.
    26. Sánchez, F.J., Manzanares, M., Andres, E.F., Tenorio, J.L., and Ayerbe, L. (1998) Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. Field Crop Res 59: 225-235.
    27. Stewart, R.R.C., and Bewley, J.D. (1980) Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiol 65:245-248.
    28. Thomson, V.P., Cunningham,S.A., Ball, M.C., and Nicotra, A.B. (2003) Compensation for herbivory by Cucumis sativus through increased photosynthetic capacity and efficiency. Oecologia 134: 167-175.
    29. Usha, T.N., and Dadlani, M. (2016) Study of free radical and peroxide scavenging enzymes and content in different vigour lots of soybean (Glycine max). Legume Res 39:233-236.
    30. von-Caemmerer, S., and Farquhar, G.D. (1984). Effects of partial defoliation, changes of irradiance during growth, short-term water stress and growth at enhanced P(CO2) on the photosynthetic capacity of leaves of Phaseolus vulgaris L. Planta 160:320-329.
    31. Wang, J., Yang, C., Han, W., and Liu, M. (2003) Effects on water-soluble carbohydrate of Artemisia frigida under different defoliation inensities. Acta Ecol Sin 23:908-913.
    32. Wright, I.J., and Westoby, M. (2003) Nutrient concentration, resorption and lifespan: leaf traits of Australian sclerophyll species. Funct Ecol 17:10-19.
    33. Yang, H.M., Wang, Z.N., and Ji, C.R. (2013) Research progress in the dynamics of carbon and nitrogen in forages after cutting and grazing. Chin J Grassl 35:102-109.
    34. Zhang, D., Lei, J., Hong, E., Lu, D., Yuan, W., Yang, Z., and Ming, C. (2016) Anti-hypoxia effects of the ethanol extract of Oxytrois ochracephala. Legume Res 39:914-920.
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