Phenotypic and molecular determination of anthracnose disease resistance in Lake Van Basin’s bean genotypes (Phaseolus vulgaris L.)

DOI: 10.18805/lr.v0i0.7858    | Article Id: LR-330 | Page : 135-142
Citation :- Phenotypic and molecular determination of anthracnose disease resistance in Lake Van Basin’s bean genotypes (Phaseolus vulgaris L.) .Legume Research-An International Journal.2018.(41):135-142

Aytekin EKINCIALP and Suat SENSOY

aytekincialp@gmail.com
Address :

Baskale Vocational School, Yuzuncu Yil University, 65080 Van, Turkey.

Submitted Date : 28-10-2016
Accepted Date : 31-01-2017

Abstract

In this study, the resistance levels to anthracnose disease [Colletotrichum lindemuthianum (Sacc. & Magnus) Lambs. Scrib.] of 92 bean genotypes collected from different parts of the Lake Van Basin were investigated by artificial inoculation and molecular markers. The resistance levels of bean genotypes to the isolate 11# of anthracnose disease were determined by classical inoculation method in a climate chamber condition, and the presence of resistance gene related markers in bean genotypes was determined by using four SCAR primers [SAS13 (950 bp, Co-42), SC08 (910 bp, Co-4), SF10 (1072 bp, Co-10), SZ04 (567 bp, Co-6)] and one RAPD [OA181500 (1500 bp, Co-15)] primer. In the artificial inoculation, the bean genotypes were evaluated according to the 0-9 scale, and the four of them having a value between 0 and 3 were found as resistant to this isolate of anthracnose, but the rest of them having a value between 4 and 9 were determined as sensitive. With molecular markers, it was found that the 82 bean genotypes had the resistant Co-42 allele; the 54 bean genotypes had the resistant Co-4 allele; the 6 bean genotypes had the resistant Co-10 allele; the 36 bean genotypes had the resistant Co-6 allele; and the 15 bean genotypes had the resistant Co-15 allele. The only bean genotype having all resistance markers was the genotype G81, whereas the bean genotypes G27, G28, G40, G76, and G86 had no resistance associated molecular markers.

Keywords

Anthracnose Artificial inoculation Bean Molecular markers.

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