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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 41 issue 1 (february 2018) : 34-40,   Doi: 10.18805/lr.v0iOF.9112

Establishment of evaluation procedure for soybean seed-flooding tolerance and its application to screening for tolerant germplasm sources 

M
Muhammad Jaffer Ali
Z
Zheping Yu
G
Guangnan Xing
T
Tuanjie Zhao
J
Junyi Gai
1Soybean Research Institute, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
Cite article:- Ali Jaffer Muhammad, Yu Zheping, Xing Guangnan, Zhao Tuanjie, Gai Junyi (2017). Establishment of evaluation procedure for soybean seed-flooding tolerance and its application to screening for tolerant germplasm sources. Legume Research. 41(1): 34-40. doi: 10.18805/lr.v0iOF.9112.

ABSTRACT

The experiments concluded 48hrs for germination testing as most appropriate stress duration. Using paper roll for recording the traits was the best method than petri dish and towel method. Among the traits relative germination rate (RGR), relative seedling length (RSL), relative root length (RRL), relative root fresh weight (RRFW) and relative root dry weight (RRDW) were proved to be flooding-responsive. RSL was chosen as the major seed-flooding indicator due to higher heritability (h2), genotypic coefficient of variation (GCV), correlation with other indicators and easier measuring procedure, while RRL was considered as the subsidiary indicator. Thus, the RSL in paper roll after 48hrs seed-flooding was recognized as standard seed-flooding testing procedure and used to evaluate breeding materials. Among the 11 cultivars, the superior seed-flooding-tolerant ones were M8206, NN1138-2 and ZXD, while ten best lines were evaluated from one hundred breeding lines.

REFERENCES

  1. Duke, S.H. and G. Kakefuda. (1981). Role of the testa in preventing cellular rupture during imbibition of legume seeds. Plant Physiol., 67:449-456. 
  2. Hao X.Y., Han. X., Ju. H. and Lin. Er-da (2010). Impact of climatic change on soybean production: A review. Chinese J. of Applied Ecology, 21: 2697-2706.
  3. Hou, F.F. and Thseng. F.S. (1991). Studies on the flooding tolerance of soybean seed: varietal differences. Euphytica, 57: 169-173. 
  4. Hou, F.F. and Thseng. F.S. (1992). Studies on the screening technique for pre-germination flooding tolerance in soybean [Glycine max]. Japanese J. of Crop Sci. 61: 447-453. 
  5. Hou, F.F.,Thseng. F.S. , Wu. S.T., and Takeda. K. (1995). Varietal differences and diallel analysis of pre-germination flooding tolerance in soybean seed. Bull. Res. Inst. Bioresour. Okayama Univ., 3: 35-41. 
  6. Kim, H., Xing. G., Wang.Y. , Zhao. T., Yu. D., Yang. S., Li.Y., Chen. S., Palmer. R.G. and Gai. J. (2014). Constitution of resistance to common cutworm in terms of antibiosis and antixenosis in soybean RIL populations. Euphytica, 196: 137-154.
  7. Kim, Y.H., Hwang. S.J., Waqas. M., Khan. A.L., Lee .J.H., Lee. J.D. and Lee. I. J.(2015). Comparative analysis of endogenous hormones level in two soybean (Glycine max L.) lines differing in waterlogging tolerance. Front. Plant Sci., 6:714-717
  8. McGraw-Hill, C. (2008). Statistix 8.1 (Analytical Software, Tallahassee, Florida). Maurice/Thomas text. 
  9. Nakayama, N., Hashimoto. S., Shimada. S., Takahashi. M., Kim.Y.H., Oya. T. and J. Arihara. (2004). The effect of flooding stress at the germination stage on the growth of soybean [Glycine max] in relation to initial seed moisture content. Japanese J. of Crop Sci., 3: 323-329. 
  10. Nguyen, V.T., Vuong. T.D., T. VanToai.T. , Lee. J.D., Wu. X., M.A, Mian. M.A. and H.T. Nguyen. (2012). Mapping of quantitative trait loci associated with resistance to and flooding tolerance in soybean. Crop Sci., 52: 2481-2493. 
  11. Reed, J.W., Nagatani.A., Elich.T.D., Fagan. M. and Chory.J. (1994). Phytochrome A and phytochrome B have overlapping but distinct functions in Arabidopsis development. Plant Physiol., 104: 1139-1149. 
  12. Sayama, T., Nakazaki. T. , Ishikawa. G., Yagasaki. K., Yamada.N., Naohiro.H. , Hirota. N. and M. Teraishi. (2009). QTL analysis of seed-    flooding tolerance in soybean (Glycine max [L.] Merr.). Plant Sci., 176: 514-521. 
  13. Shinomura, T., Nagatani. A., Chory.J. and Furuya. M. (1994). The induction of seed germination in Arabidopsis thaliana is regulated principally by phytochrome B and secondarily by phytochrome A. Plant Physiol., 104: 363-371. 
  14. Waqas, M., Khan A.L., Kang. S.M., Kim.Y.H. and Lee. I. J. (2014) Phytohormone-producing fungal endophytes and hardwood-derived biochar interact to ameliorate heavy metal stress in soybeans. Biology and Fertility of Soils., 50: 1155-1167. 
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    Research Article

    volume 41 issue 1 (february 2018) : 34-40,   Doi: 10.18805/lr.v0iOF.9112

    Establishment of evaluation procedure for soybean seed-flooding tolerance and its application to screening for tolerant germplasm sources 

    M
    Muhammad Jaffer Ali
    Z
    Zheping Yu
    G
    Guangnan Xing
    T
    Tuanjie Zhao
    J
    Junyi Gai
    1Soybean Research Institute, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
    Cite article:- Ali Jaffer Muhammad, Yu Zheping, Xing Guangnan, Zhao Tuanjie, Gai Junyi (2017). Establishment of evaluation procedure for soybean seed-flooding tolerance and its application to screening for tolerant germplasm sources. Legume Research. 41(1): 34-40. doi: 10.18805/lr.v0iOF.9112.

    ABSTRACT

    The experiments concluded 48hrs for germination testing as most appropriate stress duration. Using paper roll for recording the traits was the best method than petri dish and towel method. Among the traits relative germination rate (RGR), relative seedling length (RSL), relative root length (RRL), relative root fresh weight (RRFW) and relative root dry weight (RRDW) were proved to be flooding-responsive. RSL was chosen as the major seed-flooding indicator due to higher heritability (h2), genotypic coefficient of variation (GCV), correlation with other indicators and easier measuring procedure, while RRL was considered as the subsidiary indicator. Thus, the RSL in paper roll after 48hrs seed-flooding was recognized as standard seed-flooding testing procedure and used to evaluate breeding materials. Among the 11 cultivars, the superior seed-flooding-tolerant ones were M8206, NN1138-2 and ZXD, while ten best lines were evaluated from one hundred breeding lines.

    REFERENCES

    1. Duke, S.H. and G. Kakefuda. (1981). Role of the testa in preventing cellular rupture during imbibition of legume seeds. Plant Physiol., 67:449-456. 
    2. Hao X.Y., Han. X., Ju. H. and Lin. Er-da (2010). Impact of climatic change on soybean production: A review. Chinese J. of Applied Ecology, 21: 2697-2706.
    3. Hou, F.F. and Thseng. F.S. (1991). Studies on the flooding tolerance of soybean seed: varietal differences. Euphytica, 57: 169-173. 
    4. Hou, F.F. and Thseng. F.S. (1992). Studies on the screening technique for pre-germination flooding tolerance in soybean [Glycine max]. Japanese J. of Crop Sci. 61: 447-453. 
    5. Hou, F.F.,Thseng. F.S. , Wu. S.T., and Takeda. K. (1995). Varietal differences and diallel analysis of pre-germination flooding tolerance in soybean seed. Bull. Res. Inst. Bioresour. Okayama Univ., 3: 35-41. 
    6. Kim, H., Xing. G., Wang.Y. , Zhao. T., Yu. D., Yang. S., Li.Y., Chen. S., Palmer. R.G. and Gai. J. (2014). Constitution of resistance to common cutworm in terms of antibiosis and antixenosis in soybean RIL populations. Euphytica, 196: 137-154.
    7. Kim, Y.H., Hwang. S.J., Waqas. M., Khan. A.L., Lee .J.H., Lee. J.D. and Lee. I. J.(2015). Comparative analysis of endogenous hormones level in two soybean (Glycine max L.) lines differing in waterlogging tolerance. Front. Plant Sci., 6:714-717
    8. McGraw-Hill, C. (2008). Statistix 8.1 (Analytical Software, Tallahassee, Florida). Maurice/Thomas text. 
    9. Nakayama, N., Hashimoto. S., Shimada. S., Takahashi. M., Kim.Y.H., Oya. T. and J. Arihara. (2004). The effect of flooding stress at the germination stage on the growth of soybean [Glycine max] in relation to initial seed moisture content. Japanese J. of Crop Sci., 3: 323-329. 
    10. Nguyen, V.T., Vuong. T.D., T. VanToai.T. , Lee. J.D., Wu. X., M.A, Mian. M.A. and H.T. Nguyen. (2012). Mapping of quantitative trait loci associated with resistance to and flooding tolerance in soybean. Crop Sci., 52: 2481-2493. 
    11. Reed, J.W., Nagatani.A., Elich.T.D., Fagan. M. and Chory.J. (1994). Phytochrome A and phytochrome B have overlapping but distinct functions in Arabidopsis development. Plant Physiol., 104: 1139-1149. 
    12. Sayama, T., Nakazaki. T. , Ishikawa. G., Yagasaki. K., Yamada.N., Naohiro.H. , Hirota. N. and M. Teraishi. (2009). QTL analysis of seed-    flooding tolerance in soybean (Glycine max [L.] Merr.). Plant Sci., 176: 514-521. 
    13. Shinomura, T., Nagatani. A., Chory.J. and Furuya. M. (1994). The induction of seed germination in Arabidopsis thaliana is regulated principally by phytochrome B and secondarily by phytochrome A. Plant Physiol., 104: 363-371. 
    14. Waqas, M., Khan A.L., Kang. S.M., Kim.Y.H. and Lee. I. J. (2014) Phytohormone-producing fungal endophytes and hardwood-derived biochar interact to ameliorate heavy metal stress in soybeans. Biology and Fertility of Soils., 50: 1155-1167. 
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