Studies on genetic diversity in chickpea utilizing morphological and total seed protein markers
Citation :- Studies on genetic diversity in chickpea utilizing morphological and total seed protein markers .Legume Research.2016.(39):323-325
Genetic diversity among seventeen chickpea varieties was investigated utilizing agro morphological traits and total seed protein markers. Morphological markers grouped all the desi genotypes (9) except ICCV 10 under Cluster I, whereas kabuli genotypes (7) were grouped in three clusters. Dendogram developed based on total seed protein profile revealed that the seventeen genotypes could be grouped into two clusters broadly at a distance of 25. Similarity index between all possible pairs of seventeen genotypes showed a range of 0.074 (Vishal and ICCV-10) to 0.579 (JAKI-9218 and ICCC 37). The correspondence between different methods might be improved by analyzing more morphological characters, storage proteins and DNA markers.
Total seed proteins.
- Abou-El-enain, M. M. and Ahmed, S. M. (2012). Intraspecific diversity of Egyptian and foreign new lines of chickpea based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and isozyme markers. African .J Bioche. Res. 6: 135-139.
- Dadlani, M., Veena, V., Singh, D. P. and Anuradha, V. (1994). A comparison of field GOT and electrophoresis methods for testing genetic purity of cotton hybrid seeds. Seed Res. 22: 160-162.
- Dwevedi, K. Gaibriyal, K and Lal, M. (2009). Assessment of genetic diversity of cultivated chickpea (Cicer arietinum L.). Asian J. Agri. Sci. 1(1):7-8.
- Ghafoor, A.F.N., Gulboaz, M., Afzal, M., Ashraf and Arshad, M. (2003). Interrelationship between SDS-PAGE markers and agronomic traits in chickpea. Pak. J. Bot. 35:613-624.
- Ghafoor, A. and Arshad M.(2008). Seed protein profilling of Pisum sativum (L) germplasm using sodium dodecyl sulphate Polyacrylamide gel electrophoresis (SDS-PAGE) for investigation of biodiversity . Pak. J. Bot. 40:2315-2321.
- Jayalakshmi, V and Rufus Ronald, G. (2011). Assessment of genetic diversity for quantitative traits in chickpea (Cicer arietinum L.). J. Res. ANGRAU. 39(3):58-61.
- Kakaei, M. and Kharizi, D. (2011). Evaluation of seed storage protein patterns of ten wheat varieties using SDS – PAGE. Biharean Biol.5:116-118.
- Mahalanobis, P.C. (1936). On the generalized distance in Statistics. . Proc. of Nat. Inst. of Sci. India. 2:49-55.
- Maric, S. S. Bolaric, J. Martinck, I. Pejic and Kozumplix,V. (2004). Genetic diversity of hexaploid wheat cultivars estimated by RAPD markers, morphological traits and co-efficient of parentage. Plant Breed. 123: 366-369.
- Martinez, L. Cavagnaro, P. Masuelli, R. (2005). Evaluation of diversity among Argentina grape vine (Vitis vinifera L.) varieties using morphological data and AFLP markers. Electronic J. Biotech. 6: 37-45.
- Rao, C.R.V. (1952). Advanced Statistical Methods in Biometrical Research. John Wiley and Sons Inc., New York, pp.236-272.
- Robertson, L.D., Ocampo, B. and Singh, K.B. (1997). Morphological variation in wild annual Cicer species in comparison to the cultigen. Euphytica 95:309-319.
- Rouamba, A., Sandmeier, M., Sarr, A. and Ricroch, A. (2001). Allozyme variation within and among population of onion (Allium cepa L.) from West Africa . Theor. Appl. Genet. 103:855-861.
- Salem, K.F.M, El-zaraty, A.M. and Esmail, R.M. (2008). Assessing wheat (Triticum aestivum L.) genetic diversity using morphological characters and microsatellite markers. World J. Agric. Sci. 4: 538-544.