Cytological elucidation of somaclonal variation in grasspea (Lathyrus sativus L.)

DOI: 10.18805/lr.v39i2.9530    | Article Id: LR-3276 | Page : 177-182
Citation :- Cytological elucidation of somaclonal variation in grasspea (Lathyrus sativus L.) .Legume Research.2016.(39):177-182

Swapan K. Tripathy*, Ananya Panda and Devraj Lenka

Address :

Department of Plant Breeding & Genetics, College of Agriculture, OUAT, Bhubaneswar-751 003, India.


A cytological study of root tips, primary calli, sub-culture calli and regenerants of grass pea (Lathyrus sativus L) cv.  Nayagarh local was carried out for elucidation of the origin of tissue culture instability and somaclonal variation. Wide range of euploids, aneuploids and structural variations of chromosomes including stickiness, multipolar spindle, asymmetric chromatid separation, binucleate cells with micronuclei and chromosome bridges and fragments could be the cause for tissue culture instability. Frequency of chromosomal anomalies in calli increased with the age of subculrure and the increase was more in B5 medium supplemented with 2, 4-D than NAA. Majority of the abnormalities happens to be sieved off owing to morphogenetic selectivity. While, a few of these were carried on to regenerants as evidenced from mitotic (euploid, aneuploid and structural variation) and meiotic (interchanges and/or loss of chromosome segments following breakage events, lack of pairing resulting univalents, precocious and late separation of bivalents, anaphase laggards, conspicuous anaphase bridges with or without acentric fragments, chromosome rings, tetrad with multinucleoli) abnormalities in regenerants. Such carry-over events have been   substantiated as the origin of somaclonal variation in regenerants.


Chromosomal variation In vitro culture Lathyrus sativus L. somaclonal variation.


  1. Bairu, M.W.; Fennell, C.W. and Van Staden, J. (2006). The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. ‘Zelig’). Scientia Horticulturae, 108: 347-351.
  2. Barbier, M. and Dulieu, H.L. (1980) Genetic changes observed on tobacco plants regenerated from cotyledons by in vitro culture. Ann. Amelior Plant, 30: 321-344. 
  3. Barbier, M. and Dulieu, H.L. (1983). Early occurrence of genetic variants protoplast cultures. Plant. Sci. Lett., 29: 201-206. 
  4. Barret, P.; Brinkman, M. and Becker, M.(2006). A sequence related to rice Pong transposable element displays transcriptional activation by in vitro culture and reveals somaclonal variations in maize. Genome, 49: 1399–1407.
  5. Bayliss, M.W. (1980). Chromosomal variation in plant tissue culture. Int. Rev. Cytol., 11A: 113-144.
  6. Benzion, G. and Phillips Ronald, L. (1988). Cytogenetic stability of maize tissue cultures: a cell line pedigree analysis. Gytoplasm maizeenome, 30: 318-325.
  7. Brettell, R. I. S.; Thomas, R. and Ingram, D.S. (1980). Reversion of Texas male –sterile cytoplasm maize in culture to give fertile T-toxin resistant plants. Theor. Appl. Genet., 58: 55-58. 
  8. Chen, J.J. and Henny, R.J. (2006). Somaclonal variation: an important source for cultivar development of floriculture crops. Floriculture, ornamental and plant biotechnology. Teixeira da Silva, J. A(ed.), p. 244-253.
  9. D’Amato, F. (1975). The problem of genetic stability in plant tissue and cell cultures. In: Crop Genetic Resources for Today and Tomorrow. O. Frankel and J. G. Hawkes (eds.), Cambridge University Press, U. K., p. 333-348.
  10. De Paepe, R.; Bleton, D. and Gnangbe, F. (1981). Basis and extent of genetic variability among doubled haploid plants obtained by pollen culture in Nicotiana Sylvestris. Theor. Appl . Genet. 59: 117-184.
  11. Edallo, S.; Zucchinali, C.; Perenzin, M. and Salamini, F. (1981). Chromosomal variation and frequency of spontaneous mutation associated with in vitro culture and plant regeneration in maize. Maydica, 26: 39-56.
  12. El-bayoumi, A.S.; Kabarity, A. and Habib, A. (1979). Cytological effects of papaverine hydrochloride on root tips of Allium cepa. Cytologia, 44: 745-755.
  13. Goole (Adsense) (2010). Somaclonal variation.(http://www.articlesbase.com/leadership-article/Somaclonal variation-    3194722.html), google (Adsense0, Sep 03, 2010.
  14. Gribaudo, I.; Torello Marinoni, D.; Gambino, G.; Mannini, F.; Akkak, A. and Botta, R. (2009). Assessment of genetic fidelity in regenerants. Horticulturae, 827:131-136.
  15. Hoffman, F.; Thomas, E. and Wenzel, G. (1982). Anther culture as a breeding tool in a rape : II. Progeny analysis of androgenetic lines and induced mutants from haploid cultures. Theor. Appl. Genet., 61: 225-232.
  16. Larkin, P. and Scowcroft, W. (1981). Somaclonal variation a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet., 60: 197-214.
  17. Lorz, H. and Scowsroft, W.R. (1983). Variability among plants and their progeny regenerated from protoplasts of Su/    su heterozygotes of Nicotiana tabacum. Theor. Appl. Genet., 66: 67-75. 
  18. Mc Clintock, B. (1978). Mechanism that rapidly reorganize the genome. Stadler Genet. Symp., 10: 25-48. 
  19. McCoy, T.J., Phillips, R.L. and Rines, H.W. (1982). Cytogenetic analysis of plants regenerated from oat(Avena sativa) tissue cultures: High frequency of partial chromosome loss. Can. J. Genet. & Cytol., 24: 37-50.
  20. Mohanty, S; Panda, M.; Subudhi, E and Nayak, S. (2008). Plant regeneration from callus culture of Curcuma aromatica and in vitro detection of somaclonal variation through cytophotometric analysis. Biologia Plantarum, 52:783-786.
  21. Mujib, A., Banerjee, S. and Dev Ghosh, P.(2007). Callus induction, somatic embryogenesis and chromosomal instability in tissue culture raised hippeastrum (Hippeastrum hybridum cv. United Nations). Propagation of Ornamental Plants 7:169-174.
  22. Ogihara, Y. (1981). Tissue culture in Haworthia: IV. Genetic characterization of plants regenerated from callus. Theor. Appl. Genet., 60: 353-363.
  23. Orton, T.J. (1980). Chromosomal variability in tissue cultures and regenerated plants of Hordeum. Theor. Appl. Genet. 56: 101-112.
  24. Orton, T. J. (1983). Spontaneous electrophoretic and chromosomal variability in callus cultures and regenerated plants of celery. Theor. Appl. Genet. 67: 17-24.
  25. Phillips, R.L.; Kaepplert, S.M. and Olhoft, P. (1994). Genetic instability of plant tissue cultures: Breakdown of normal controls. Proc. Nati. Acad. Sci., USA, 91: 5222-5226.
  26. Prat, D. (1983). Genetic variability induced in Nicotiana Sylvestris by protoplast culture. Theor. Appl . Genet., 64: 223-230.
  27. Sanal P.K. and Mathur V.L. (2004).Chromosomal Instability in Callus Culture of Pisum sativum Plant Cell Tissue and Organ Culture, 78(3):267-271).
  28. Sarmento, D.; Martins, M. and Oliveira, M.M. (2005). Evaluation of somaclonal variation in almond using RAPD and ISSR. Options Méditerranéennes, Série A, 63: 391-395.
  29. Somaroo, B.H. and Grant, N.F. (1972). Meiotic chromosome behaviour in tetraploid hybrids between synthetic Lotus amplidiploids and L. corniculatus. Can. J. Genet. Cytol., 14: 57-64. 
  30. Sunderland, N. (1977) Nuclear Cytology. In: Plant Cell and Tissue Culture. Vol. 2. Ed. H.E. Street (ed.), Univ. California Press, Berkely, 2: 177-205.
  31. Talukdar D. (2009). Development of cytogenetic stocks through induced mutagenesis in grass pea ( Lathyrus sativus ): Current status and future prospects in crop improvement. Grain Legume, 54: 30-31.
  32. Tawakley, M.; Sudhavani, A.K. and Reddy, G.M. (1992 ). Chromosomal instability in callus cultures of wild and cultivated genotypes. Indian J. Expt. Biol., 30: 628-631.
  33. Tripathy, S.K. and Cole, C.R. (2001). Genotypic Response to plant regeneration in Lathyrus sativus L. Plant Sci. Res. 23:17-20.

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