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volume 38 issue 4 (august 2015) : 452-456,   Doi: 10.5958/0976-0571.2015.00129.0

Molecular characterization of mung bean (Vigna radiata L.) genotypes through RAPD, ISSR and SSR markers

J
J.M. Sanghani
B
B.A. Golakiya
K
K.K. Dhedhi
S
S.V. Patel
1Department of Biotechnology, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India.
Cite article:- Sanghani J.M., Golakiya B.A., Dhedhi K.K., Patel S.V. (2025). Molecular characterization of mung bean (Vigna radiata L.) genotypes through RAPD, ISSR and SSR markers. Legume Research. 38(4): 452-456. doi: 10.5958/0976-0571.2015.00129.0.

ABSTRACT

The RAPD, ISSR and SSR marker system was applied to the 11 mung bean genotypes. The RAPD data showed that primer OPC-11 was the best primer resulting good amplification with maximum PIC value (0.893). Dendrogram constructed using the RAPD data clearly distinguished all genotypes. It revealed that genotypes MEHA and HUM-1 found in one cluster and shared maximum 75.00% similarity; however, genotype PUSHA VISHAL have out grouped from other 10 genotypes and shared minimum 45.5% similarity. The ISSR data showed that primer 810 was the best primer exhibit good amplification with maximum PIC value (0.907). Jaccard’s similarity coefficient ranged from 0.229 to 0.886. The ISSR results indicated that maximum similarity of 88.6% was found between IPM-2-3 and GM-4, while minimum similarity of 22.9% was obtained between PUSHA VISHAL and K-851. From the SSR data it was found that the primer VR-304 was the best primer showing good amplification with maximum PIC value (0.746). Jaccard’s similarity coefficient ranged from 0.607 to 0.952. So the SSR data revealed that MEHA showed maximum variability compared to other ten genotypes. The combined RAPD, ISSR and SSR analysis revealed that out of eleven genotypes IPM-2-3 and HUM-1 showed maximum 80.9% similarity. The lowest similarity of 40.2% was found between PUSHA VISHAL and K-851.

REFERENCES

  1. Afzal M. A., Haque M. M. and Shanmugasundaram S. (2004) Random amplified polymorphic DNA (RAPD) analysis of selected mungbean (Vigna radiata L. Wilczek) cultivars. Asian J. Pl. Sci. 3, 20–24.
  2. Arunachalam V. (1981) Genetic distance in plant breeding. Indian J. Genet. 14. 226–236.
  3. Bisht I. S., Mahajan R. K. and Kawalkar T. G. (1998) Diversity in mungbean (Vigna radiata L.Wilczek) germplasm collection and its potential use in crop improvement. Ann. Appl. Biol. 132, 301– 312.
  4. Chattopadhyay K., Ali M. N., Sarkar H. K., Mandal N. and Bhattacharyya S. (2005) Diversity analysis by RAPD and ISSR markers among the selected mungbean [Vigna radiata (L.) Wilczek] genotypes. Indian J. Genet. Pl. Breed. 65, 173–175.
  5. Lakhanpaul S., Chadha S., Bhat K. V. and Chadha S.(2000) Random amplified polymorphic DNA analysis in Indian mungbean (Vigna radiata L. Wilczek) cultivars. Genetica 109, 227–234.
  6. Manivannan N., Murugan E., Viswanathan P. L. and Dhanakodi C. V. (1998) Genetic divergence in greengram. Legume Res. 21, 131– 133.
  7. Mignouna H. D., Ng N. Q., Ikea J. and Thotapilly G. (1998) Genetic diversity in cowpea as revealed by random amplified polymorphic DNA. J. Genet. Breed. 53, 151–159.
  8. Newbury H. J. and Ford-Lloyd B. V. (1993) The use of RAPD for assessing variation in plants. Plant Growth Regulation 12, 43–51.
  9. Orozco-Castillo C., Chalmers K. J., Waugh R. and Powell W. (1994) Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theor. Appl. Genet. 87, 934–940.
  10. Paredes M., Becerra V., Rojo C., Del Pojo A., Ovalle C. and Aronson J. (2002 ) Ecotypic differentiation in Medicago polymorpha L. along an environmental gradient in central Chile. RAPDs studies shows little genetic divergence. Euphytica 123, 431–439.
  11. Ramanujam S. (1981) Biometrical basis for yield improvement in mungbean. In: Procedings 1st International Mung bean Symposium, (ed. R. Cowell) pp. 210 AVRDC, Shanhua.
  12. Rohlf F. J. (1998). NTSYS-pc. Numerical taxonomy and multivariate analysis system, version 2.02. Exter Software, Setauket, NY.
  13. Saini A., Reddy K. S. and Jawali N. (2004) Evaluation of longprimers for AP-PCR analysis of mungbean [Vigna radiata (L.) Wilczek]: Genetic relationships and fingerprinting of some genotypes. Indian J. Biotechnol. 3, 511–518.
  14. Santalla M., Power J. B. and Davey M. R. (1998) Genetic diversity in mungbean germplasm revealed by RAPD markers. Plant Breed. 117, 473–478.
  15. Skroch P. W., dos Santos J. B. and Nienhuis J. (1992) Genetic relationships among Phaseolus vulgaris genotypes based on RAPD marker data. Annu. Rep. Bean Improv. Coop. 35, 23–24.
  16. Virk P. S., Ford-Lloyd B. V., Jackson M. T. and Newbury H. J. (1995) Use of RAPD for the study of diversity within plant germplasm collections. Heredity 74, 170–179.
  17. Wachira F. N.,Waugh R., Hackett C. A. and PowellW. (1995) Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers. Genome 38, 201–210.
  18. Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A. and Tingey S. V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6231–6235.
  19. Yee E., Kidwell K. K., Sills G. R. and Lumpkin, T. A. (1999) Diversity among selected Vigna angularis (Azuki) accessions on the basis of RAPD and AFLP markers. Crop Sci. 39, 268–275.
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    volume 38 issue 4 (august 2015) : 452-456,   Doi: 10.5958/0976-0571.2015.00129.0

    Molecular characterization of mung bean (Vigna radiata L.) genotypes through RAPD, ISSR and SSR markers

    J
    J.M. Sanghani
    B
    B.A. Golakiya
    K
    K.K. Dhedhi
    S
    S.V. Patel
    1Department of Biotechnology, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India.
    Cite article:- Sanghani J.M., Golakiya B.A., Dhedhi K.K., Patel S.V. (2025). Molecular characterization of mung bean (Vigna radiata L.) genotypes through RAPD, ISSR and SSR markers. Legume Research. 38(4): 452-456. doi: 10.5958/0976-0571.2015.00129.0.

    ABSTRACT

    The RAPD, ISSR and SSR marker system was applied to the 11 mung bean genotypes. The RAPD data showed that primer OPC-11 was the best primer resulting good amplification with maximum PIC value (0.893). Dendrogram constructed using the RAPD data clearly distinguished all genotypes. It revealed that genotypes MEHA and HUM-1 found in one cluster and shared maximum 75.00% similarity; however, genotype PUSHA VISHAL have out grouped from other 10 genotypes and shared minimum 45.5% similarity. The ISSR data showed that primer 810 was the best primer exhibit good amplification with maximum PIC value (0.907). Jaccard’s similarity coefficient ranged from 0.229 to 0.886. The ISSR results indicated that maximum similarity of 88.6% was found between IPM-2-3 and GM-4, while minimum similarity of 22.9% was obtained between PUSHA VISHAL and K-851. From the SSR data it was found that the primer VR-304 was the best primer showing good amplification with maximum PIC value (0.746). Jaccard’s similarity coefficient ranged from 0.607 to 0.952. So the SSR data revealed that MEHA showed maximum variability compared to other ten genotypes. The combined RAPD, ISSR and SSR analysis revealed that out of eleven genotypes IPM-2-3 and HUM-1 showed maximum 80.9% similarity. The lowest similarity of 40.2% was found between PUSHA VISHAL and K-851.

    REFERENCES

    1. Afzal M. A., Haque M. M. and Shanmugasundaram S. (2004) Random amplified polymorphic DNA (RAPD) analysis of selected mungbean (Vigna radiata L. Wilczek) cultivars. Asian J. Pl. Sci. 3, 20–24.
    2. Arunachalam V. (1981) Genetic distance in plant breeding. Indian J. Genet. 14. 226–236.
    3. Bisht I. S., Mahajan R. K. and Kawalkar T. G. (1998) Diversity in mungbean (Vigna radiata L.Wilczek) germplasm collection and its potential use in crop improvement. Ann. Appl. Biol. 132, 301– 312.
    4. Chattopadhyay K., Ali M. N., Sarkar H. K., Mandal N. and Bhattacharyya S. (2005) Diversity analysis by RAPD and ISSR markers among the selected mungbean [Vigna radiata (L.) Wilczek] genotypes. Indian J. Genet. Pl. Breed. 65, 173–175.
    5. Lakhanpaul S., Chadha S., Bhat K. V. and Chadha S.(2000) Random amplified polymorphic DNA analysis in Indian mungbean (Vigna radiata L. Wilczek) cultivars. Genetica 109, 227–234.
    6. Manivannan N., Murugan E., Viswanathan P. L. and Dhanakodi C. V. (1998) Genetic divergence in greengram. Legume Res. 21, 131– 133.
    7. Mignouna H. D., Ng N. Q., Ikea J. and Thotapilly G. (1998) Genetic diversity in cowpea as revealed by random amplified polymorphic DNA. J. Genet. Breed. 53, 151–159.
    8. Newbury H. J. and Ford-Lloyd B. V. (1993) The use of RAPD for assessing variation in plants. Plant Growth Regulation 12, 43–51.
    9. Orozco-Castillo C., Chalmers K. J., Waugh R. and Powell W. (1994) Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theor. Appl. Genet. 87, 934–940.
    10. Paredes M., Becerra V., Rojo C., Del Pojo A., Ovalle C. and Aronson J. (2002 ) Ecotypic differentiation in Medicago polymorpha L. along an environmental gradient in central Chile. RAPDs studies shows little genetic divergence. Euphytica 123, 431–439.
    11. Ramanujam S. (1981) Biometrical basis for yield improvement in mungbean. In: Procedings 1st International Mung bean Symposium, (ed. R. Cowell) pp. 210 AVRDC, Shanhua.
    12. Rohlf F. J. (1998). NTSYS-pc. Numerical taxonomy and multivariate analysis system, version 2.02. Exter Software, Setauket, NY.
    13. Saini A., Reddy K. S. and Jawali N. (2004) Evaluation of longprimers for AP-PCR analysis of mungbean [Vigna radiata (L.) Wilczek]: Genetic relationships and fingerprinting of some genotypes. Indian J. Biotechnol. 3, 511–518.
    14. Santalla M., Power J. B. and Davey M. R. (1998) Genetic diversity in mungbean germplasm revealed by RAPD markers. Plant Breed. 117, 473–478.
    15. Skroch P. W., dos Santos J. B. and Nienhuis J. (1992) Genetic relationships among Phaseolus vulgaris genotypes based on RAPD marker data. Annu. Rep. Bean Improv. Coop. 35, 23–24.
    16. Virk P. S., Ford-Lloyd B. V., Jackson M. T. and Newbury H. J. (1995) Use of RAPD for the study of diversity within plant germplasm collections. Heredity 74, 170–179.
    17. Wachira F. N.,Waugh R., Hackett C. A. and PowellW. (1995) Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers. Genome 38, 201–210.
    18. Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A. and Tingey S. V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6231–6235.
    19. Yee E., Kidwell K. K., Sills G. R. and Lumpkin, T. A. (1999) Diversity among selected Vigna angularis (Azuki) accessions on the basis of RAPD and AFLP markers. Crop Sci. 39, 268–275.
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