RAPD markers for genetic characterization in mungbean [Vigna radiata (L.) Wilczek]

DOI: 10.5958/0976-0571.2015.00023.5    | Article Id: LR-2979 | Page : 279-288
Citation :- RAPD markers for genetic characterization in mungbean [Vigna radiata (L.) Wilczek].Legume Research-An International Journal.2015.(38):279-288
Swapan K. Tripathy*, Suchinnata S. Sardar, Sasmita Dash, Shovina Pal, Tanuja Acharya, Bhumika Ray Mohapatra, Anath B. Das and Gyana R. Rout swapankumartripathy@gmail.com
Address : Sinha Molecular Breeding Laboratory, Department of Plant Breeding & Genetics, College of Agriculture, OUAT, Bhubaneswar-751 003, India.

Abstract

Information on genetic variability and relatedness among 20 selected high yielding Vigna genotypes were assessed using RAPD profiles. Ten RAPD primers generated 81 amplicons resulting 98.7% polymorphism. 5-12 bands were produced per primer with an average of 8.1 bands and the maximum being resolved using primer OPD 08. All primers revealed 100% polymorphic loci except the primer OPD 15 which produced one monomorphic amplicon (500bp). Jaccard’s similarity coefficient values indicated >80% genomic homology between the T-series mutants of Dhauli (parent variety). T 2-1 had shown two specific bands of amplicon size 750bp and 920bp produced by primer OPD 18. Similarly, a 900bp band amplified by primer OPA-8 was specific to Purusattam local whereas, it was absent in all other local land races. RAPD analysis confirmed that wild accession of mungbean (Vigna radiata var. Sublobata) is more close to mungbean particularly local land races than urdbean. The UPGMA cluster analysis distinguished each single variety resulting single variety cluster at even 0.86 similarity index. Further, the RAPD analysis revealed high divergence of UG 218, LGG460 and TCR 20 from AB 2557, ML 267 and Pusa 172. These genotypes could serve as valuable genetic materials in recombination breeding.

Keywords

Genetic diversity Mungbean Molecular characterization RAPD markers.

References

  1. Afzal, M. A.; Haque, M. M. and Shamnmuga Sundaram, S. (2004). Random amplified polymorphic DNA analysis of selected mungbean cultivars. Asian Journal of Plant Sciences, 3 : 20-24.
  2. Ali, M. and Kumar, S. (2006). Advances in mungbean and urdbean. IIPR kanpur Report , p 1-19.
  3. Benko-Iseppon, A. M.; Winter, P.; Huettel, B.; Staginnus, C.; Muehlbauer, F.J. and Kahl G. (2003). Molecular markers closely linked to fusarium resistance genes in chickpea show significant alignments to pathogenesis related genes located on Arabidopsis chromosomes 1 and 5. Theor. Appl. Genet., 107: 379-386.
  4. Betal, S.; Chowdhury, P. R.; Kundu, S. and Raychaudhuri, S.S. (2004). Estimation of genetic variability of Vigna radiata cultivars by RAPD analysis. Bilogia Plantarm, 48: 205-209.
  5. Bhat, K.V.; Lakhanpaul, S. and Chadha, S. (2005). Amplified fragment length polymorphism (AFLP) analysis of genetic diversity in Indian mungbean [Vigna radiata (L.) Wilczek] cultivars. Indian J. Biotech., 4: 56-64.
  6. Caetano-Anolles, G. and Gresshof, P.M. (1994). DNA amplification finger printing using arbitarily mini-hairpin oligonucleotide primers. Biotechnology, 12: 619-623.
  7. Chattopadhyay, K.; Ali, M. N.; Sarkar, H. K.; Mandal, N. and Bhattacharya S. (2005). Diversity analysis by RAPD and ISSR markers among the selected mungbean (Vigna radiata (L.) Wilczek) genotypes. Indian J. Genet. Plant Breed., 65: 173-175.
  8. Chowdhary, K. V.; Davierwala, A. P.; Gupta, V. S.; Ranjekar, P. K. and Govila, O. P. (1998). Genotype identification and assessment of genetic relationships in pearl millet (Pennisetum glaucum (L.) R. Br) using microsatellites and RAPDs. Theor. Appl. Genet., 97 : 154-162.
  9. Dhanaraj, A. L.; Rao, E. V. V. B.; Swamy, K .R. M.; Bhat M.G.; Prasad, D. T. and Sondur, S. N. (2002). Using RAPD to access the diversity in Indian cashew (Anacardium occidentale L.) germplasm. J. Hort. Sci. Biotechnol, 77:41-47.
  10. de Condolle, A. (1986). Origin of cultivated plants. Hafner Publishing, New York, p. 1-100.
  11. Doyle , J. J. and Doyle, J. L. (1990). Isolation of DNA from plant tissue. Focus, 12: 13-15.
  12. Fang, J., Chao, C. T.; Roberts, P. A. and Ehlers. J. D. (2007). Genetic diversity of cowpea (Vigna unguiculata (L.) Walp.) in four west African and USA breeding programs as determined by AFLP analysis. Gen. Res. Crop Evol,, 54:1119-1209.
  13. Fernandez, M.E.; Fingueiras, A M. and Benito, C. (2002). The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin. Theor. Appl. Genet., 104: 845-851.
  14. Ghafoor, A.; Ahmed, Z. and Afzal, M (2005). Use of SDS-PAGE markers for determining quantitative trait loci in blackgram (Vigna mungo (L.) Hepper) germplasm. Pak. J. Bot., 37: 263-269.
  15. Galvan, M. Z.; Bornet, B.; Balatti, P. A. and Branchard, M. (2003). Inter simple sequence repeat (ISSR) marker as a tool for the assessment of both genetic diversity and gene pool origin in common bean (Phaseolus vulgaris L.). Euphytica, 132: 297-301.
  16. Ghallab, K. H.; Ekram; A. M., Afiah, S. A and Ahmed, S.M. (2007). Characterization of some superior mungbean genotypes on the agronomic and biochemical genetic levels. Egyptian J. Desert Res. 57: 1-11.
  17. Hu, J. and Quaros, C.F. (1991). Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Reports, 10: 505-511.
  18. Jaccard, P. (1908). Nouvellesrecherches sur la distribution florale. Bulletin De La Societe Vaudoise Des sciences Naturelles. 44: 223-270.
  19. Kaga, A; Tomooka, N.; Egawa, Y., Hosaka K. and Kamijima O. (1996). Species relationships in the subgenus Ceratotropis (genus vigna) as revealed by RAPD analysis. Euphytica, 88: 17-24.
  20. Karp, A.O.; Seberg, A. and Buiatti M..(1996). Molecular techniques in the assessment of botanical diversity. Annal Bot., 78: 143-149.
  21. Ko, H. L.; Cowan, D. C.; Henry, R. J.; Graham, G. C.; Blakeney, A. B. and Lewin, L. G. (1994). Random amplified polymorphic DNA analysis of Australian rice (Oryza sativa L.) varieties. Euphytica, 80: 179-189.
  22. Lavanya, G. R.; Srivastava, J. and Ranade, S.A. (2008). Molecular assessment of genetic diversity in mungbean germplasm. Journal of Genetics, 87(1): 65-74.
  23. Lakhanpaul, S.; Chadha, S. and Bhat K.V. (2000). Random amplified polymorphic DNA (RAPD) analysis in Indian mungbean (V. radiata (L.) Wilczek) cultivars. Genetica, 109: 227-234.
  24. MacKill, D.J. (1995). Classifying japonica rice cultivars with RAPD markers. Crop Science, 35: 889-894.
  25. Muthusamy, S.; Kanagarajan, S.; and Ponnusamy, S. (2008). Efficiency of RAPD and ISSR marker system in accessing genetic variation of rice bean (Vigna umbellata) landraces, Electronic Journal of Biotechnology, 11: 1-10.
  26. Newburry, H. J. and Lioyd, B. V. F. (1993). The use of RAPD for assessing variation in plants: Plant Growth Regulation. 12: 43-51.
  27. Rakshit, S.; Winter, P.; Benko-Iseppon, A.M.; Muchlbauer F.J. and Kahl, G. (2003). DAF marker tightly linked to a major locus for Aschochyta blight resistance in chickpea (Cicer arietinum L.). Euphytica, 132: 23-30.
  28. Saini, M.; Singh, S.; Hussain, Z. and Yadav, A. (2010). RAPD analysis in mungbean(Vigna radiata(L.) wilczek): I. Assessment of genetic diversity. Indian J. Biotechnology, 9:137-146.
  29. Saini, A.; Reddy, K. S. and Jawali, N. (2004). Evaluation of long primers AP-PCR analysis of mungbean (Vigna radiata (L.) wilczek): Genetic relationship and fingerprinting of some genotypes. Indian J. Biotechnology, 3:511-518.
  30. Samarajeewa, P. K.; Nanayakkara, H. L.V.; Ekanayake, E. M. D. S. N. and Sumanasinghe, V. A. (2002). RAPD analysis of genetic relationships of wild and cultivated vigna species. Annals. of Srilanka. Dept. of Agril., 4: 215-226.
  31. Souframanien, J. and Gopalkrishna, T. (2004). A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers. Theor. and Appl. Genet., 109: 1687-1693.
  32. Tomooka, N.; Lairungreang, C. and Egawa, Y. T. C. (1995). Taxonomic position of wild Vigna species collected in Thailand based on RAPD analysis. In : Mungbean germplasm. JIRCAS Working Report No.2: 31-40.
  33. Vavilov, N. I. (1926). The origin, variation, immunity and breeding of cultivated plants (translated by Starr K. Chester). Waltham, Mass, USA. Chronica Bot., 13: 28-30.
  34. Winter, P.; Benko-Iseppon, A.M.; Huttel, B.; Ratnaparkhe, M.; Tullu, A.; Sonnante, G.; Ptaff, T.; Tekeoglu, M.; Santra, D.; Sant, V. J.; Rajesh, P. N.; Kahl, G. and Muehlbauer, F. J. (2000). A linkage map of the chickpea (Cicer arietinum L.) genome based on recombinant inbred lines from a C. arietinum X C. reticulatum cross: Localization of resistance genes for Fusarium wilt races 4 and 5. Theor. Appl. Genet., 101: 1155-1163.
  35. Xu, R.Q.; Tomooka, N.; Vaughan, A. D. and Doi, K. (2000). The vigna angularis complex: Genetic variation and relationships revealed by RAPD analysis and their implications for in-situ conservation and domestication. Genetic Resources and Crop Evolution. 47: 123-134.
  36. Zukovskij, P. M. (1962).Cultivated Plants and Their Wild Relatives. Commonwealth Agril. Bureau, London. P. 5-98.

Global Footprints