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volume 38 issue 4 (august 2015) : 519-523,   Doi: 10.5958/0976-0571.2015.00086.7

Enhancement of regeneration in moth bean [Vigna aconitifolia (Jacq) Marechal] through gamma irradiation

S
S. Narayan
S
S.N. Saxena
M
M.L. Jakhar
R
R. Sharma
1Plant Biotechnology Centre, Agricultural Research Station, Beechwal-334 006, India.
Cite article:- Narayan S., Saxena S.N., Jakhar M.L., Sharma R. (2025). Enhancement of regeneration in moth bean [Vigna aconitifolia (Jacq) Marechal] through gamma irradiation. Legume Research. 38(4): 519-523. doi: 10.5958/0976-0571.2015.00086.7.

ABSTRACT

An experiment was carried out to assess effect of gamma irradiation on morphogenetic potential of callus derived from primary leaves of seedlings of moth bean seeds. Germination percentage was decreased as the dose of gamma irradiation increased from 200 to 800 Gy. Regeneration potential of callus derived from primary leaves of the treated seeds was increased with lower doses of gamma irradiations (200 Gy and 400 Gy). Exposure to 400 Gy resulted in marked increase in number of shoot bud primordial and number of shoots per explant. Further, the plantlets regenerated from such explants produced more number of flowers under in vitro conditions. Genotype Jadia was found highly responsive to in vitro mutagenesis.

REFERENCES

  1. Ahloowalia B.S. (1998). In vitro techniques and mutagenesis for the improvement of vegetatively propagated plants. In: Somaclonal Variation and Induced Mutations in Crop Improvement. (ed. S. M. Jain, D. S. Brar and B. S. Ahloowalia . Kluwer Academic Publishers. The Netherlands. Pp 293-310.
  2. Ananthaswamy S.; Bhatnagar P.S. and Singh, C. (1971). Differential dose response of wheat genotypes to mutagenesis with gamma rays. Biovigyanum., 18:111-113.
  3. Eapen S., Gill R. and Rao P.S. (1986). Tissue culture studies in moth bean. Curr. Sci., 55: 707-709.
  4. Gahukar S.J. and Jambhale N.D. (2000). Callus induction and regeneration in Saccharum cultivars as influenced by mutagen treatments. J. Maharashtra agric. Univ., 25: 219-220.
  5. Gao M.W.; Cai Q.H. and Liang, Z.Q. (1992). In vitro culture of hybrid indica rice combined with mutagenesis. Plant Breed., 108: 104-110.
  6. Giridharan M.P. and Balakrishnana S.S. (1992). Gamma rays induced variability in vegetative and floral characteristics of ginger. Ind. Cocoa Arecanut species J., 15:68-72.
  7. Gupta M.N.; Laxmi V.; Dixit V.S. and Srivastava, S.N. (1982). Gamma rays induced variability in Costus speciosus. Progr. Hort. 14:193-197.
  8. Katoch P.C.; Massar J.E. and Plaha, P. (1992). Effect of gamma irradiation on variation in segregating generations of F2 seeds of rice. Indian J. Genet., 52:213-218.
  9. Kaushik N. (2004). Effect of some photochemicals on in vitro flowering in Vigna aconitifolia. M.Sc. thesis Department of Biotechnology, RAU, Bikaner.
  10. Kumari R. (1996). Assessment of mono and combined mutagenesis on the extent of plant injury in M1 of Vicia faba L. J. Nuc. Agril. Bio., 25: 51-53.
  11. Larkin P.I, and Scowcroft W.R. (1981). Somaclonal variation- A novel source of variability from cell cultures for plant improvement. Theo. and Appl. Genet., 60: 197-214.
  12. Liu Y.G.; Mitsukawa N.; Oosumi T. and Whittier R. (1995). Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J., 8:457-463.
  13. Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bioassays with tabacco tissue culture. Physiologia Plantarum. 15: 473-497.
  14. Nichterlein K., Bohlmann H., Nielen S. and Maluszynski, M. (2000). Achievements and trends of using induced mutations in crop improvement. DAE-BRNS symposium, Bhabha Atomic Researech Centre., Mumbai, India. Pp.27-35.
  15. Patil S. and Bawankar S. (2000). Mutation studies in Lathyrus sativus. DAE-BRNS symposium, Bhabha Atomic Researech Centre., Mumbai, India. Pp.117-123.
  16. Saxena S.N., Rohit K. and Sharma R. (2005). Prospects of manipulating in vitro flowering in moth bean for crop improvement. J. Arid Legumes 2:210-214.
  17. Sharma R.C. and Majumdar J.R. (1982). Studies of flower shedding behaviour in moth bean. Sci. Cult., 48:65-66.
  18. Sinha S.S.N. and Godward M.B.F. (1972). Radiation studies in Lens culinaris. Indian J. Genet., 32:331-339.
  19. Srivastava R.K.; Sandhu A.S. and Gosal S.S. (2001). Effect of in vitro mutagenesis on plant regeneration in Citrus aurantifolia. Mutation Breed. News Letter. 45:48-50.
  20. Wakode M.M., Nandanwar R.S. and Patil G.P. (2000). Radiation induced mutagenesis in soybean. DAE-BRNS symposium, Bhabha Atomic Researech Centre., Mumbai, India. Pp.111-116.
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    volume 38 issue 4 (august 2015) : 519-523,   Doi: 10.5958/0976-0571.2015.00086.7

    Enhancement of regeneration in moth bean [Vigna aconitifolia (Jacq) Marechal] through gamma irradiation

    S
    S. Narayan
    S
    S.N. Saxena
    M
    M.L. Jakhar
    R
    R. Sharma
    1Plant Biotechnology Centre, Agricultural Research Station, Beechwal-334 006, India.
    Cite article:- Narayan S., Saxena S.N., Jakhar M.L., Sharma R. (2025). Enhancement of regeneration in moth bean [Vigna aconitifolia (Jacq) Marechal] through gamma irradiation. Legume Research. 38(4): 519-523. doi: 10.5958/0976-0571.2015.00086.7.

    ABSTRACT

    An experiment was carried out to assess effect of gamma irradiation on morphogenetic potential of callus derived from primary leaves of seedlings of moth bean seeds. Germination percentage was decreased as the dose of gamma irradiation increased from 200 to 800 Gy. Regeneration potential of callus derived from primary leaves of the treated seeds was increased with lower doses of gamma irradiations (200 Gy and 400 Gy). Exposure to 400 Gy resulted in marked increase in number of shoot bud primordial and number of shoots per explant. Further, the plantlets regenerated from such explants produced more number of flowers under in vitro conditions. Genotype Jadia was found highly responsive to in vitro mutagenesis.

    REFERENCES

    1. Ahloowalia B.S. (1998). In vitro techniques and mutagenesis for the improvement of vegetatively propagated plants. In: Somaclonal Variation and Induced Mutations in Crop Improvement. (ed. S. M. Jain, D. S. Brar and B. S. Ahloowalia . Kluwer Academic Publishers. The Netherlands. Pp 293-310.
    2. Ananthaswamy S.; Bhatnagar P.S. and Singh, C. (1971). Differential dose response of wheat genotypes to mutagenesis with gamma rays. Biovigyanum., 18:111-113.
    3. Eapen S., Gill R. and Rao P.S. (1986). Tissue culture studies in moth bean. Curr. Sci., 55: 707-709.
    4. Gahukar S.J. and Jambhale N.D. (2000). Callus induction and regeneration in Saccharum cultivars as influenced by mutagen treatments. J. Maharashtra agric. Univ., 25: 219-220.
    5. Gao M.W.; Cai Q.H. and Liang, Z.Q. (1992). In vitro culture of hybrid indica rice combined with mutagenesis. Plant Breed., 108: 104-110.
    6. Giridharan M.P. and Balakrishnana S.S. (1992). Gamma rays induced variability in vegetative and floral characteristics of ginger. Ind. Cocoa Arecanut species J., 15:68-72.
    7. Gupta M.N.; Laxmi V.; Dixit V.S. and Srivastava, S.N. (1982). Gamma rays induced variability in Costus speciosus. Progr. Hort. 14:193-197.
    8. Katoch P.C.; Massar J.E. and Plaha, P. (1992). Effect of gamma irradiation on variation in segregating generations of F2 seeds of rice. Indian J. Genet., 52:213-218.
    9. Kaushik N. (2004). Effect of some photochemicals on in vitro flowering in Vigna aconitifolia. M.Sc. thesis Department of Biotechnology, RAU, Bikaner.
    10. Kumari R. (1996). Assessment of mono and combined mutagenesis on the extent of plant injury in M1 of Vicia faba L. J. Nuc. Agril. Bio., 25: 51-53.
    11. Larkin P.I, and Scowcroft W.R. (1981). Somaclonal variation- A novel source of variability from cell cultures for plant improvement. Theo. and Appl. Genet., 60: 197-214.
    12. Liu Y.G.; Mitsukawa N.; Oosumi T. and Whittier R. (1995). Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J., 8:457-463.
    13. Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bioassays with tabacco tissue culture. Physiologia Plantarum. 15: 473-497.
    14. Nichterlein K., Bohlmann H., Nielen S. and Maluszynski, M. (2000). Achievements and trends of using induced mutations in crop improvement. DAE-BRNS symposium, Bhabha Atomic Researech Centre., Mumbai, India. Pp.27-35.
    15. Patil S. and Bawankar S. (2000). Mutation studies in Lathyrus sativus. DAE-BRNS symposium, Bhabha Atomic Researech Centre., Mumbai, India. Pp.117-123.
    16. Saxena S.N., Rohit K. and Sharma R. (2005). Prospects of manipulating in vitro flowering in moth bean for crop improvement. J. Arid Legumes 2:210-214.
    17. Sharma R.C. and Majumdar J.R. (1982). Studies of flower shedding behaviour in moth bean. Sci. Cult., 48:65-66.
    18. Sinha S.S.N. and Godward M.B.F. (1972). Radiation studies in Lens culinaris. Indian J. Genet., 32:331-339.
    19. Srivastava R.K.; Sandhu A.S. and Gosal S.S. (2001). Effect of in vitro mutagenesis on plant regeneration in Citrus aurantifolia. Mutation Breed. News Letter. 45:48-50.
    20. Wakode M.M., Nandanwar R.S. and Patil G.P. (2000). Radiation induced mutagenesis in soybean. DAE-BRNS symposium, Bhabha Atomic Researech Centre., Mumbai, India. Pp.111-116.
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