COWPEA [VIGNA UNGUICULATA (L.) WALP.] SEED PROTEINS: HETEROGENEITY IN TOTAL PROTEINS AND PROTEIN FRACTIONS

DOI: 10.5958/j.0976-0571.37.1.009    | Article Id: LR-2882 | Page : 62-67
Citation :- COWPEA [VIGNA UNGUICULATA (L.) WALP.] SEED PROTEINS: HETEROGENEITY IN TOTAL PROTEINS AND PROTEIN FRACTIONS.Legume Research-An International Journal.2014.(37):62-67
Prachi Gupta, Rohtas Singh*, S. Malhotra, K.S. Boora and H.R. Singal rohtas_bot@rediffmail.com
Address : Centre for Plant Biotechnology, CCS Haryana Agricultural University, New Campus, Hisar-125 004, India

Abstract

Eleven genotypes of V.  unguiculata were screened for variability in total proteins and various protein fractions viz. albumins, globulins, prolamins and glutelins by using SDS-polyacrylamide gel electrophoresis. The electrophoretic pattern showed the molecular heterogeneity among total proteins as well as various protein fractions. The molecular weights of protein bands obtained by SDS-PAGE varied between 10 to 141.3, 15.85 to 147.9, 10 to 125.9, 7.94 to 56.23 and 10 to 79.43 kDa for total proteins, albumins, globulins, prolamins and glutelins, respectively. Most of the bands were common to the different genotypes indicating their close relationship with one another. However, a few bands unique to some particular genotypes were also observed showing the varietal differences. Cluster analysis based on total proteins and various protein fractions provided evidence for differences among the  varieties collected from different agroclimatic zones of India. The observations revealed that seed protein polymorphism provides a useful information regarding variability among genotypes under investigation which could be helpful for breeding purposes in the improvement of protein quality and quantity in grain legumes.

Keywords

Cowpea Genotypes Protein fractions Seeds SDS-PAGE Vigna unguiculata.

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