SUITABILITY OF SSR MARKER FOR ESTABLISHING DISTINCTIVENESS IN FRENCH BEAN VARIETIES (PHASEOLUS VULGARIS L.)

DOI: 10.5958/0976-0571.2014.00643.2    | Article Id: LR-2880 | Page : 353-358
Citation :- SUITABILITY OF SSR MARKER FOR ESTABLISHING DISTINCTIVENESS IN FRENCH BEAN VARIETIES (PHASEOLUS VULGARIS L.).Legume Research-An International Journal.2014.(37):353-358
Neetu Mall* and H.S. Chawla nmmallneetu@gmail.com
Address : Department of Genetics and Plant Breeding, G B Pant Univ. of Agric. & Tech, Pantnagar, 263 145, India

Abstract

Eleven French bean varieties (Phaseolous vulgaris L.) were studied for SSR molecular marker utilizing ten SSR primers. It revealed 32 alleles in 11 varieties with an average of 3.2 alleles / locus. The number of alleles per locus varied from 1 to 5. The size of allele amplified varied from 50 to 500bp. Cluster analysis was done using pairwise Jaccard’s similarity co-efficient with similarity value ranges from 37% to 94%. The PIC value in the present study ranged from 0 to 0.79, with an average of 0.37 per primer pair. Specific loci were obtained in four local varieties namely Chakrata Local-2 PGR FB 2-07, Bhura Lal Swant, Lal Thumri Swant and Chitkabra Lal Jhulu Swant by 10 SSR primers which could unambiguously discriminate these varieties. When all the morphological and SSR molecular marker were considered then eight varieties could be distinguished. Study showed that for establishing distinctiveness in varieties, besides morphological characters some other DNA based markers might also be considered.

Keywords

French bean DUS Jaccard’s similarity index SSR marker.

References

  1. Anonymous. (2007). General guidelines to the examination of DUS and the development of harmonized descriptors of plant varieties. Plant Variety Journal India, 1 (1): 192-201.
  2. Cao, W.G., Huel P.S.G., Chibbar R.N. (1998). Genetic diversity within spelta and macha wheats based on RAPD analysis. Euphytica. 104: 181-189.
  3. Dixit, G.P., Katiyar, P.K. and Singh B.B. (2011). Characterization of lentil (Lens culinaris Medik.) varieties based on morphological traits. J. Food Legume , 24 (3): 194-197.
  4. Doyle, J.J. and Doyle, J.L. (1990). Isolation of plant DNA form fresh tissues. Focus. 12: 13-15.
  5. Duran, L.A., Blair M.W., Giraldo M.C., Macchiavelli R., Prophete E., Nin J.C. and J.S.Beaver (2005). Morphological and Molecular Characterization of Common bean landraces and cultivars from the Caribbean. Crop Sci.45: 1320-1328.
  6. FAO. (2010). The State of Food and Agriculture. www.fao.org/
  7. Gaitan-S., Duque, M.C., Edwards, K.J. and Tohme, J.(2002). Microsatellite repeats in common bean (Phaseolus vulgaris): Isolation, characterization, and cross- species amplification in Phaseolus sp. Crop Sci. 42: 2128 2136.
  8. Giancola, S., Poltri S.M., Lacaze P. and Hopp H.E. (2002). Feasibility of integration of molecular and morphological descriptors in a real case study of a plant variety protection system for soyabean. Euphytica.127:95-113.
  9. Jaccard, P. (1908). Bull. Soc. Vaudoise Sci.Nature. 44: 223-270.
  10. Joshi, D.C., Shrotria P.K., Singh Ravindra, Srivastava M.K. and Chawla H.S. (2011). Assessment of RAPD and ISSR marker systems for establishing distinctiveness of forage Sorghum (Sorghum bicolor (L.) Moench) varieties as additional descriptors for plant variety protection. Indian J. Genet. 71 (1): 1-11.
  11. Joshi, A. and Chawla, H.S. (2010). Biochemical and molecular markers for establishing distinctiveness of aromatic rice (Oryza sativa L.) varieties. Indian J. Genet. 70 (1): 58-64.
  12. Katiyar, P.K., Dixit G.P. and Singh B.B. (2008). Morphological characterization of greengram (Vigna radiate)varieties and their application. Indian J. Agri. Sci. 78(5):54-56.
  13. McCouch, S.R., X.L. Chen, O. Panaud, S. Temnykh, Y.B. Xu, Y.G. Cho, N. Huang, T. Ishii & M. Blair, (1997).Microsatellite marker development, mapping and application in rice genetics and breeding. Plant Mol. Biol. 35: 89-99.
  14. Patra, N. and Chawla, H.S. (2010). Biochemical and RAPD molecular markers for establishing distintiveness of basmati rice (Oryza sativa L.) varieties as additional descriptors for plant variety protection. Indian J. Biotechnology, 9: 371-377.
  15. Peter, S., Horacek, J., Dostalova, R. and Hybl, M.(2008). Variety discrimination in pea (Pisum satium L.) by molecular, biochemical and morphological markers. J. Appl. Genet. 49 (2): 155-166.
  16. Rohlf, F.J. (2005). NT-SYS-pc: Numerical Taxonomy and Multivariate Analysis System. Version 2.11W. Exter Software: Setauket, NY.
  17. Sarikamis, G.Yasar F., M..Bakir, K.Kazan and Eggiil, A. (2009).Genetic characterization of green bean (Phaseolus vulgaris) genotypes from eastern Turkey. Genetics and Mol. Res. 8 (3): 880-887.
  18. Stachel, M., Lelly T., Grausgruber H., Vollman J. (2000). Application of microsatellite in wheat for studying genetic differentiation caused by selection for adaptation and use. Theor. Appl. Genet. 100: 242-248.
  19. Temnykh, S., Declerk, G., Luckashova, A., Lipovich, L., Cantinhour, S. and Mc Couch, S. (2001).Computational and experimental analysis of micro satellites in rice (Oryza sativa L.): frequency and length variation, transposon association, and genetic marker potential. Genome Research, 11: 1441-52.
  20. UPOV-BMT, (2002). BMT/36/10 Progress report of the 36th session of the technical committee, the technical working parties and working group on biochemical and molecular techniques and DNA- profiling in particular, Geneva.
  21. Yu, K., Park, S.J., Poysa, V. and Gepts, P. (2003). Integration of simple sequence repeat markers into a molecular linkage map of common bean (Phaseolus vulgaris L.). J. Hered. 91: 429-434.

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