Legume Research-An International Journal

IDENTIFICATION OF PEANUT (Arachis hypogaea L.) VARIETIES THROUGH CHEMICAL TESTS AND ELECTROPHORESIS OF SOLUBLE SEED PROTEINS

Article Id: LR-2816 | Page : 475-483
Citation :- IDENTIFICATION OF PEANUT (Arachis hypogaea L.) VARIETIES THROUGH CHEMICAL TESTS AND ELECTROPHORESIS OF SOLUBLE SEED PROTEINS .Legume Research-An International Journal.2013.(36):475-483
P.S. Rao*, M. Bharathi and K. Bayyapu Reddy sampalrao@gmail.com
Address : Seed Research and Technology Centre, A.N.G.R. Agricultural University, Hyderabad-500 030, India

Abstract

The ability to characterize and identify plant varieties is fundamental to several aspects of seed trade. Thirty five genotypes of peanut (Arachis  hypogaea L.) which  are  in  demand  and  in  seed  production  chain  were  characterized by utilizing the biochemical tools like total  soluble  seed  proteins  and seed and seedling  characteristics  for  their  reaction  to  NaOH, KOH, GA3  and  2,4-D tests. It  was  possible  to  differentiate  between  all  the  cultivars  into  different  groups  like  light  brown   and  dark  brown  and  also  low, moderate  and  high  response  of  coleoptile  to  respective  chemicals. The  banding  pattern  of  soluble  seed  proteins  by  SDS-PAGE  was  quite  different  for each  of  these  genotypes  individually. The number of  bands  in  different  molecular  weight  ranged  from  11  to  28. The   genotype GG 12 exhibited least number (11) of bands and the genotype GG 20 produced maximum number (28) of bands. The  seed  protein profiles of the genotypes  revealed  the qualitative and quantitative  variation  among  the  different  genotypes, thus indicating  the usefulness of  electrophoretic  variation  in  varietal  identification.

Keywords

Electrophoresis Peanut Protein bands Relative mobility Seed key.

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