Photosynthetic and antioxidant variability in soybean genotypes under Cadmium stress   

DOI: 10.18805/lr.v0i0.7853    | Article Id: LR-278 | Page : 470-477
Citation :- Photosynthetic and antioxidant variability in soybean genotypes under Cadmium stress .Legume Research-An International Journal.2017.(40):470-477

Faheema Khan* and Asma Al-Huqail
Address :

Department of Botany and Microbiology, College of Science King Saud University, Riyadh 11495, Kingdom of Saudi Arabia

Submitted Date : 17-02-2016
Accepted Date : 10-02-2017


Soybean, the worldwide main source of oil and high protein feeds for the livestock sector has a high cadmium (Cd) accumulation capacity. With this background, the hydroponic culture experiments were conducted to investigate the effects of different concentrations of Cd (0-100 µM) on growth, water relations, photosynthetic variables, oxidative stress, and antioxidant response in two soybean genotypes P-218 and P-898. Ten days old seedlings were subjected to (0-100 µM CdCl2) for 15 days. The results indicated that the growth of genotype P-218 was not affected significantly upto 75 µM CdCl2 treatment growth of P-828 was reduced significantly beyond 25 µM CdCl2 treatments. Cd toxicity caused severe impairments in photosynthetic variables like photosynthetic rate, chlorophyll fluorescence and chlorophyll content, in P-898 than in P-218. The activities of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) were higher in P-218 than in P-828 at various levels of Cd treatments. Chlorophyll fluorescence measurements revealed that non-photochemical quenching increased in P-218 and decreased in P-828 whereas the electron transport rate increased under Cd stress in P-828 and decreased in P-218. It is concluded that tolerance capacity of P-218 against Cd can be associated with the capability of this genotype in keeping an active photosynthetic system and strong antioxidant defense system.


CdCl2 Chlorophyll fluorescence Lipid peroxidation Oxidative stress Soybean.


  1. Aebi, H. (1984). Catalase in vitro. Meth.Enzymol., 105: 121–126.
  2. Ahammed, G.J., Choudhary S.P, Chen, S., Xia, X., Shi, K., Zhou, Y. and Yu J. (2013). Role of brassino- steroids in alleviation of phenanthrene–cadmium co-contamination-induced photosynthetic inhibition and oxidative stress in tomato. J. Exp. Bot., 
  3. 64: 199–213.
  4. Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts, Polyphenoloxidase in Beta vulgaris. Plant Physiol., 24: 1-15.
  5. Beauchamp, C. and Fridovich. I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem., 44: 276–287.
  6. Bell, M.J., McLaughlin, M.J., Wright, G.C. and Cruickshank, J. (1997). Inter and intraspecific variation in accumulation of cadmium by peanut, soybean, and navy bean. Aust. J. Agric. Res., 48: 1151–1160.
  7. Bor, M., Özdemir, F. and Türkan, I. (2003). The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet (Beta vulgaris L.) and wild beet (Beta maritima L.) Plant Science., 164: 77–84.
  8. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
  9. Chen, Y.X., He, Y.F., Luo, Y.M., Yu, Y.L., Lin, Q. and Wong, M.H. (2003). Physiological mechanism of plant roots exposed to cadmium. Chemosphere., 50: 789–793.
  10. Da Rosa Correa, A.X., Rorig, L.R., Verdinelli, M.A., Cotelle, S., Ferard J.F. and Radetski, C.M. (2006). Cadmium phytotoxicity: Quantitative sensitivity relationships between classical endpoints and antioxidative enzyme biomarkers. Sci. Total Environ., 357: 120–127.
  11. De Oliveira, J.A., Oliva, M.A., Cambraia, J. and Venegas, V.H.A. (1994). Absorption, accumulation and distribution of cadmium by two soybean cvs. Rev. Bras. Fisiol.Veg., 6: 91 95.
  12. Dixit, V., Pandey, V. and Syam, R (2001). Differential oxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv. Azad). J. Exp. Bot., 52: 1101–1109.
  13. Dong, J., Wu, F.B. and Zhang, G.P. (2006). Influence of cadmium on antioxidant capacity and four microelement concentrations in tomato seedlings (Lycopersicon esculentum). Chemosphere., 64: 1659–1666.
  14. Foyer, C.H. and Halliwell, B. (1976). The presence of glutathione and glutathione reductase in chloroplast: a proposed role in ascorbic acid metabolism. Planta., 133: 21–25.
  15. Foyer, C.H., Lelandais, M., Galap, C. and Kunert, K.J. (1991). Effects of photosynthesis by ozone. Photosynthesis Research., 39: 439–51.
  16. Giannopolitis, C.N. and Ries, S.K. (1977). Superoxide dismutase: Occurrence in higher plants Plant Physiol., 59: 309–314.
  17. Gill, S.S. and Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem., 48: 909–930.
  18. Gill, S.S., Hasanuzzaman, M., Nahar, K., Macovei, A. and Tuteja, N. (2013). Importance of nitric oxide in cadmium stress tolerance in crop plants. Plant Physiol. Biochem., 63: 254-261.
  19. Groppa, M.D., Ianuzzo, M.P., Rosales, E.P., Vázquez, S.C. and Benavides, M.P. (2012). Cadmium modulates NADPH oxidase activity and expression in sunflower leaves. Biologia Plantarum., 56: 167–171.
  20. Hassan, M.J., Shao, G. and Zhang, G.P. (2005). Influence of cadmium toxicity on antioxidant enzymes activity in rice cultivars with different grain Cd accumulation. J. Plant Nutr., 28: 1259 1270.
  21. Heath, R.L. and Packer, L. (1968). Photoperoxidation in isolated chloroplasts I. Kinetic and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys., 125: 189–198.
  22. Heyno, E., Klose, C. and Krieger-Liszkay, A. (2008). Origin of cadmium-induced reactive Oxygen species production: mitochondrial electron transfer versus plasma membrane NADPH oxidase. New Phytologist., 179: 687–699.
  23. Hiscox, J.D. and Israelstam, G.F. (1979). A method for extraction of chlorophyll from the leaf without maceration. Can J Plant Sci., 57: 1332-1334.
  24. Hoagland, D.R. and Arnon, D.S. (1950). The water culture method for growing plants without soil. Calif. Agric. Exp. Stat. Circ., 347:1-32.
  25. Inouhe, M., Ninimiya, S., Tohoyama, H., Joho, M. and Murayama, T. (1994). Different characteristics of roots in calcium-tolerance and Cd binding complex formation between mono-and dicotyledonous plants. J. Plant Res., 107: 201 207.
  26. Larbi, A., Morales, F., Abadía, A., Gogorcena, Y., Lucena, J., Murayama, J. and Abadía, J. (2002). Effects of Cd and Pb in sugar beet plants grown in nutrient solution: induced Fe deficiency and growth inhibition. Funct. Plant Biol., 29: 1453–1464.
  27. Maksymiec, W. and Krupa, Z. (2006). The effects of short-term exposition to Cd, excess Cu ions and jasmonate on oxidative stress appearing in Arabidopsis thaliana. Environ Exp Bot., 57:187–194.
  28. Metwally, A., Safronova, V.I., Belimov, A.A. and Dietz, K.J. (2005). Genotypic variation of the response to cadmium toxicity in Pisum sativum L. J Exp Bot., 56: 167 178.
  29. Miller, J.F. (2006). Registration of three low cadmium (HA 448, HA 449, and RHA 450) confection sunflower genetic stocks. Crop Science., 46: 489–90.
  30. Nakano, Y. and Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol., 22: 867–880.
  31. Oncel, I., Kele, Y. and Ustun, A.S. (2000). Interactive effects of temperature and heavy metal stress on the growth and some biochemical compounds in wheat seedlings. Environ Pollut., 107: 315 320.
  32. Palaniswamy, R. and Padma, P.R. (2011). Free radical s scavenging activity of Mojorana hortensis leaves. Anc Sci Life., 30: 96–99.
  33. Perilli, P., Mitchell, L.G., Grant, C.A. and Pisante, M. (2010). Cadmium concentration in durum wheat grain (Triticum turgidum) as influenced by nitrogen rate, seeding date and soil type. J. Sci. Food Agr., 90: 813–822.
  34. Qian, H., Li, J., Sun, L., Chen, W., Sheng, G.D., Liu, W. and Fu, Z. (2009). Combined effect of copper and cadmium on Chlorella vulgaris growth and photosynthesis-related gene transcription. Aquatic Toxicology., 94: 56–61.
  35. Schreiber, U., Schliwa, U. and Bilger, W. (1986). Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynthesis Research.., 10: 51-62.
  36. Shamsi, I.H., Lixi, J., Wei, K., Jilani, G.H. Hua, S.J. and Zhang, G.P. (2010). Alleviation of cadmium toxicity in soybean by potassium supplementation. J. Plant Nutr., 33: 1926–1938. 
  37. Shamsi, I.H., Zhang, G., Hu, H., Xue, Q., Hussain, N., Ali, E., Shen, Q., Zheng, W., Zhang, Q., Liu, X., Jabeen, Z. and Lin, X. (2014). Assessment of the Hazardous Effects of Cd on Physiological and Biochemical Characteristics of Soybean Genotypes Int. J. Agric. Biol., 16 (1): 41-38.
  38. Shigeoka, S., Nakano, Y. and Ktaoka, S. (1979). The biosynthetic pathway of L-ascorbic acid in Euglena gracihs. J Nutr Sci Vitaminol., 25: 299-307.
  39. Singh, A. and Prasad, S.M. (2011). Reduction of heavy metal load in food chain: technology assessment. Rev. Environ. Sci. Biotechnol., 10: 199-214.
  40. Singh, H.P., Batish, D.R., Kaur, G., Arora, K. and Kohli, R.K. (2008). Nitric oxide (as sodium nitroprusside) supplementation ameliorates Cd toxicity in hydroponically grown wheat roots. Envýron Exp Bot., 63: 158–167.
  41. Stolt, P., Asp, H. and Hultin, S. (2006). Genetic variation in wheat cadmium accumulation on soils with different cadmium concentrations. J Agron Crop Sci., 192: 201–208.
  42. Sugiyama, M., Ae, N. and Hajika, M. (2011). Developing of a simple method for screening soybean seedling cadmium accumulation to select soybean genotypes with low seed cadmium. Plant Soil., 341: 413–422.
  43. Xing, W., Huang, W.M. and Liu, G.H. (2010). Effect of excess iron and copper on physiology of aquatic plant Spirodela polyrrhiza (L.). Environ. Toxicol., 25: 103–112.
  44. Xu, J., Wang, W.Y., Yin, H.X., Liu, X.J., Sun, H. and Mi, Q. (2010). Exogenous nitric oxide improves antioxidative capacity and reduces auxin degradation in roots of Medicago truncatula seedlings under cadmium stress. Plant Soil., 32: 321–330. 

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