Legume Research

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Research Article

Legume Research, volume 39 issue 5 (october 2016) : 722-728

Phenotypic characterisation of rhizobia nodulating different morphotypes of Vachellia karroo

N. Ngwenya1, G.E. Zharare1, A.K. Basson2, M.S. Mthembu*2
1<p>Department of Microbiology, University of Zululand,&nbsp;Private Bag X1001, KwaDlangezwa, 3886 South Africa.</p>
Cite article:- Ngwenya1 N., Zharare1 G.E., Basson2 A.K., Mthembu*2 M.S. (2016). Phenotypic characterisation of rhizobia nodulating differentmorphotypes of Vachellia karroo . Legume Research. 39(5): 722-728. doi: 10.18805/lr.v0iOF.3542.

ABSTRACT

Vachellia karroo is an important pasture legume which fixes nitrogen through its symbiotic relationship with rhizobia. Thirty two indigenous rhizobia were isolated from four different morphotypes of V. karroo obtained from four agro-ecologically diverse areas of South Africa. Phenotypic characterization of isolates revealed that they have the same colony morphology. Numeric analysis of the isolates’ phenotypic characteristics produced six clusters at 83% level of relative similarity. High variation of the isolates growth in different temperatures, salt concentration, pH, antibiotic resistance and heavy metal resistance was also noted. Carbon source utilization was generally the same for all isolates. Isolates that tolerated a wide range of test conditions are recommended as potentially highly competitive V. karroo inoculants for use in various climatic and soil conditions.

REFERENCES


  1. Alexandre, A., Laranjo, M. and Oliveira, S. (2006). Natural populations of chickpea rhizobia evaluated by antibiotic resistance profiles and molecular methods. Microbial Ecology, 51:128-136.

  2. Alshaharani, T. S. and Shetta, N. D. (2015). Phenotypic and biochemical characterization of root nodule bacteria naturally associated with woody tree legumes in Saudi Arabia. Journal of Environmental Biology, 36: 363.

  3. Bakhoum, N., Le Roux, C., Diouf, D., Kane, A., Ndoye, F., Fall, D., Duponnois, R., Noba, K., Sylla, S. N. and Galiana, A. (2014). Distribution and Diversity of Rhizobial Populations Associated with Acacia senegal (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions. Open Journal of Forestry, 4: 136.

  4. Bansal, M., Kukreja, K., Sunrja, S. and Dudeja, S. (2014). Symbiotic effectivity of high temperature tolerant mungbean (Vigna radiata) rhizobia under different temperature conditions. Int. J. Curr. Microbiol. App. Sci, 3, 807-821.

  5. Bedi, M. K. and Naglot, A. (2011). Characterization of Rhizobium isolated from root nodules of Trifolium alexandrinum. Journal of Agricultural Technology, 7: 1705-1723.

  6. Boukhatem, Z. F., Domergue, O., Bekki, A., Merabet, C., Sekkour S., Bouzza, F., Duponnois, R., De Lajudie, P. and Galiana, A. (2012). Symbiotic characterization and diversity of rhizobia associated with native and introduced acacias in arid and semi-arid regions in Algeria. FEMS Microbiology Ecology, 80: 534-547.

  7. Chagas Junior, A. F., De Oliveira, L. A., De Castro, H. G., Cornelio, G. D. L., Dos Santos, G. R., Chagas, L. F. B. and Costa, J. D. L. (2013). Isolation and phenotypic characterization of rhizobia that nodulate cowpea in the Cerrado in Tocantins State, Brazil. Journal of Biotechnology and Biodiversity, 4: 3.

  8. Cooper, J. E. and Scherer, H. W. (2012). Chapter 16 - Nitrogen Fixation. In: PETRA, M. (ed.) Marschner’s Mineral Nutrition of Higher Plants (Third Edition). San Diego: Academic Press.

  9. Dreyfus, B. and Dommergues, Y. (1981). Nodulation of Acacia species by fast-and slow-growing tropical strains of Rhizobium. Applied and Environmental Microbiology, 41: 97-99.

  10. Graham, P. H., Draeger, K. J., Ferrey, M. L., Conroy, M. J., Hammer, B. E., Martinez, E., Aarons, S. R. and Quinto, C. (1994). Acid pH tolerance in strains of Rhizobium and Bradyrhizobium, and initial studies on the basis for acid tolerance of Rhizobium tropici UMR1899. Canadian Journal of Microbiology, 40: 198-207.

  11. Hayward, R. (2004). The acacia tree: a sustainable resource for Africa. An overview of research conducted by Oxford Forestry Institute, the Natural Environmental Research Council (NERC) Centre for Ecology and Hydrology and the University of Duncadee 1986 and 2004. Rowes Printers , P.366.

  12. Jida, M. and Assefa, F. (2014). Effects of Acidity on Growth and Symbiotic Performance of Rhizobium leguminosarum bv. viciae Strains Isolated from Faba Bean Producing Areas of Ethiopia. Science, Technology and Arts Research Journal, 3: 26-33.

  13. Laurette, N. N., Maxémilienne, N. B., Henri, F., Souleymanou, A., Kamdem, K., Albert, N., Dieudonné, N. and François-    Xavier, E. (2015). Isolation and Screening of Indigenous Bambara Groundnut (Vigna Subterranea) Nodulating Bacteria for their Tolerance to Some Environmental Stresses. American Journal of Microbiological Research, 3: 65-75.

  14. Maâtallah, J., Berraho, E., Munoz, S., Sanjuan, J. and Lluch, C. (2002). Phenotypic and molecular characterization of chickpea rhizobia isolated from different areas of Morocco. Journal of applied microbiology, 93: 531-540.

  15. Macedo, M., Resende, A., Garcia, P., Boddey, R., Jantalia, C., Urquiaga, S., Campello, E. and Franco, A. (2008). Changes in soil C and N stocks and nutrient dynamics 13 years after recovery of degraded land using leguminous nitrogen-    fixing trees. Forest Ecology and Management, 255: 1516-1524.

  16. Moreira, F. M., Siqueira, J. O. and Brussaard, L. (2006). Soil biodiversity in Amazonian and other Brazilian ecosystems, CABI publishing, Cambridge, 304 pp.

  17. Mpepereki, S., Makonese, F. and Wollum, A. (1997). Physiological characterization of indigenous rhizobia nodulating Vigna unguiculata in Zimbabwean soils. Symbiosis (Philadelphia, Pa.)(USA), 22: 275-292.

  18. Pearce, F. (2009). The nitrogen fix: breaking a costly addiction. Yale Environment, 360.

  19. Rai, R., Dash, P. K., Mohapatra, T. and Singh, A. (2012). Phenotypic and molecular characterization of indigenous rhizobia nodulating chickpea in India. Indian journal of experimental biology, 50: 340.

  20. Rao, N. S. (1991). Current developments in biological nitrogen fixation, Cambridge University Press.

  21. Saikia, R. (2008). Microbial Biotechnology, New India Publishing, pag 144.

  22. Satyanarayana, T. and Johri, B. (2005). Microbial diversity: current perspectives and potential applications, IK International Pvt Ltd.

  23. Somasegaran, P. and Hoben, H. J. (1994). Collecting nodules and isolating rhizobia. Handbook for Rhizobia, Methods in Legume-Rhizobium Technology, P 7-23. Springer.

  24. Taylor, C. and Barker, N. (2012). Species limits in Vachellia (Acacia) karroo (Mimosoideae: Leguminoseae): Evidence from automated ISSR DNA “fingerprinting”. South African Journal of Botany, 83: 36-43.

  25. Zahran, H. H. (2001). Rhizobia from wild legumes: diversity, taxonomy, ecology, nitrogen fixation and biotechnology. Journal of Biotechnology, 91, 143-153.

     
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