A cladistic analysis of medical Astragalus penduliflorus Lam. complex (Leguminosae: Papilionoideae) in China and its taxonomic implications

DOI: 10.18805/lr.v0iOF.11189    | Article Id: LR-264 | Page : 698-703
Citation :- A cladistic analysis of medical Astragalus penduliflorus Lam. complex (Leguminosae: Papilionoideae) in China and its taxonomic implications .Legume Research-An International Journal.2016.(39):698-703

Xiaolin Liu1*, Lei Duan2* and Zhaoyang Chang3

bingdch@163.com
Address :

Institude of Applied Chemistry and Biological Engineering, Weifang Engineering Vocational College, Qingzhou, Shandong 262500, China.

Submitted Date : 25-12-2015
Accepted Date : 28-05-2016

Abstract

To test the taxonomy of medical Astragalus penduliflorus complex and specific status of A. petrovii, eleven accessions (covering four species) in China were sampled herein for an AFLP study. A total of 346 informative polymorphic bands from eight primers pairs were obtained, dendrogram was generated using UPGMA analysis. The results indicated A. membranaceus is more morphologically and generically diverse than A. mongholicus on the populational level. Both of them and the former species A. minhensis were degraded as three varieties of A. penduliflorus. In the light of morphological and cladistic evidence, a new combination, A. penduliflorus ssp. mongholicus var. petrovii was proposed based on former species A. petrovii.

Keywords

AFLP Astragalus penduliflorus complex Astragalus petrovii Taxonomy.

References

  1. Chen, C.J. and Zhu, X.Y. (1990). Karyotype of Astragalus penduliflorus Lam. complex (Leguminosae) and its cytotaxonomic significance. Cathaya 2: 139-150.
  2. Dong, T.X., Ma, X.Q., Clarke, C., Song, Z.H., Ji, Z.N., Lo, C.K. and Tsim, W.K. (2003). Phylogeny of Astragalus in China: molecular evidence from the DNA sequences of 5S rRNA spacer, ITS, and 18S rRNA. J. Agric. Food Chem. 51: 6709-6714.
  3. Doyle, J.J. and Doyle, J.L. (1990). Isolation of DNA from small amounts of plant tissues. BRL focus 12: 13-15.
  4. Ho, Y.C. and Fu, K.T. (1993). Astragalus subgen. Phaca. In: Fu KT (Ed) Flora Reipublicae Popularis Sinica, Vol. 42(1). Science Press, Beijing, 114-168.
  5. Huang, X.A., Dong, M.F., Yan, X.Y. and Shang, F.D. (2011). Analysis of genetic relationships among species of Lycoris Herb. by AFLP markers. Chin. tradit. herbal drug 42: 148-152.
  6. Hsiao, P.K. (1964). Studies on the original plant and pharmacognosy of traditional Chinese medicine: Huangqi. Acta Pharm. Sin. 11: 117-123.
  7. Komarov, V.L. (1946). Flora of the U.S.S.R., Vol. 12. Jerusalem: Israel Program for Scientific Translations, 28-31.
  8. Lavin, M., Herendeen, P.S. and Wojciechowski, M.F. (2005). Evolutionary rates analysis of Leguminosae implicates a rapid diversification of lineages during the Tertiary. Syst. Biol. 54: 575-594.
  9. Liu, X.L. (2008). Taxonomical study on Astragalus in Qinling Range. MS Thesis, Northwest A&F University, China.
  10. Lock, J.M. and Schrire, B.D. (2005). Galegeae. In: Lewis, G., B. Schrire, B. Mackinder and M. Lock (eds). Legumes of the World. Kew: Royal Botanic Garden, 475-487.
  11. LPWG (The Legume Phylogeny Working Group). (2013). Legume phylogeny and classification in the 21st century: Progress, prospects and lessons for other species-rich clades. Taxon 62: 217-248. 
  12. Qian, D., Chen, M., Yuan, Q.J., Cui, H.G., Huang, L.Q. and Xiao, P.G. (2009). A taxonomic study on the original plant of Radix Astragali. Acta Pharm. Sin. 44: 1429-1433.
  13. Shi, G.R. (2003). Cluster analysis for karyotype of Astragalus penduliflorus Lam. complex (Leguminosae). Bull. Bot. Res., Harbin 23: 220-223. 
  14. Sneath, P.H.A. and Sokal, R.R. (1973). Numerical taxonomy. The principles and practice of numerical classification. San Francisco: WH Freeman, 1-573. 
  15. The State Pharmacopoeia Commission. (2010). Pharmacopoeia of the People’s Republic of China. Vol. I. Beijing: The medicine science and technology press of China, 212.
  16. Ulziykhutag, N. (1996). Generis Astragalus L. (Fabaceae) taxa nova ex Asia centrali. Novosti Sist. Nizsh. Rast. 30: 109.
  17. Wang, A. (2013). Genetic diversity analysis of Astragalus mongholicus and A. membranaceus. PhD Thesis, Minzu University of China, China.
  18. Xu, L.R. and Podlech, D. (2010). Astragalus. In: Wu, Z.Y., D.Y. Hong and P.H. Raven (eds). Flora of China, Vol. 10. Science Press, Beijing & Missouri Botanical Garden Press, St. Louis, 328-453.
  19. Yakovlev, G.P. (1996). Astragalus. In: Yakovlev, G.P., A.K. Sytin and Y.R. Roskov (eds). Legumes of northern Eurasia: A check-list. Richmond: Royal Botanic Gardens, Kew, 97-268.
  20. Zhang, R. (2014). Analysis on genetic diversity of Radix Astragali by ISSR marker. MS Thesis, Shanxi Agricultural University, China.
  21. Zhang, M.L., Kang, Y., Zhou, L.H. and Podlech, D. (2009). Phylogenetic origin of Phyllolobium with a further implication for diversification of Astragalus in China. J. Integr. Pl. Biol. 51: 889-899.
  22. Zhao, Y.Z. (2006). Taxonomy and floristic geographical distribution of the Chinese medicinal Huangqi. Bull. Bot. Res., Harbin 26: 532-538.
  23. Zhou, Y.Q. (2009). Study of different sources Astragalus with AFLP and metabolite profiling. MS Thesis, Fujian Agriculture and Forestry University, China.
  24. Zhu, Y.X. (1996). An additional note on karyotype analysis of Astragalus penduliflorus complex (Fabaceae) Lam. Guihaia 16: 61-63.
  25. Zhu, Y.X. (2003). Revision of the Astragalus penduliflorus complex (Leguminosae-Papilionoidae). Nordic J. Bot. 23: 283-294.
  26. Zhu, X.Y. and Chen, J.R. (1995). A new species of Astragalus from China. Guihaia 15:112-113.
  27. Zou, Y.P., Ge, S. and Wang, X.D. (2001). Molecular markers in systematic and evolutionary botany. Beijing: Science press, 36-97.
     

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