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Current IssueEditorial BoardOnline First ArticlesArchive

volume 38 issue 1 (february 2015) : 1-8,   Doi: 10.5958/0976-0571.2015.00001.6

An expressed sequence tags analysis for leaves of Chinese milk vetch (Astragalus sinicus)

X
X. Zhang*
J
J. Wang
K
K. Cao
C
C. Xu
W
W. Cao
1Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
Cite article:- Zhang* X., Wang J., Cao K., Xu C., Cao W. (2025). An expressed sequence tags analysis for leaves of Chinese milk vetch (Astragalus sinicus). Legume Research. 38(1): 1-8. doi: 10.5958/0976-0571.2015.00001.6.

ABSTRACT

Chinese milk vetch (Astragalus sinicus) is one of the most important green manure crops in temperate zone countries. To identify the genes involved in plant growth, a cDNA library was constructed from the mature leaves of Chinese milk vetch, and expressed sequence tag analysis was performed. Sequencing of the cDNA clones generated 1,027 high-quality expressed sequence tags clustered total 693 unigenes which included 110 Contigs and 583 Singlets. A total of 198 unigenes were most similar to Medicago truncatula genes. Genes related to photosynthesis were highly expressed in our library. The most abundant gene which had 42 clones was annotated as Ribulose bisphosphate carboxylase small chain with the function of carbon utilization by fixation of carbon dioxide. The results suggested that this high-quality library could be a good resource for understanding molecular events of Chinese milk vetch leaf growth, and the full-length clones could be used for crop improvement studies in the future.

REFERENCES

  1. Adams, M. D., Kelley, J. M. Gocayne, J. D. Dubnick M. and Polymeropoulos M.H. (1991). Complementary DNA sequencing: expressed sequence tags and human genome project. Science, 252: 1651–1656.
  2. Amanda P. Cavanagh David S. and Kubien (2014). Can phenotypic plasticity in Rubisco performance contribute to photosynthetic acclimation? Photosynth Res., 119:203–214.
  3. Bellafiore S, Barneche F, Peltier G and Rochaix JD. (2005). State transitions and light adaptation require chloroplast thylakoid protein kinase STN7. Nature, 433: 892–895.
  4. Bouck AMY, Vision T. (2007). The molecular ecologist’s guide to expressed sequence tags. Mol Ecol., 16:907–924.
  5. Elfstrand S, Hedlund K, Martensson A. (2007). Soil enzyme activities, microbial community composition and function after 47 years of continuous green manuring. Appl Soil Ecol., 35:610–621.
  6. Farquhar GD, Sv Caemmerer and Berry JA. (1980). A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species. Planta, 149:78–90.
  7. Firn R and Jones C. (2009). A Darwinian view of metabolism: molecular properties determine fitness. J Exp Bot., 60:719–    726.
  8. Kim SY, Lee BJ, Kim JH, Oh SH, Hwang WH, Hwang DY, Ahn JW, Oh BJ and Ku YC. (2007). The timing for incorporating Chinese milk vetch plant into soil for natural reseeding in the southern part of Korean peninsula. Korean J Crop Sci., 52:127.
  9. Mary E. Knight, John A. Ray and Wolfgang Schuch (1992). Isolation of a gene from maize encoding a chlorophyll a/b-    binding protein. Plant Mol Biol., 19: 533-536.
  10. Na CS, Lee YH, Hong SH, Jang CS, Kang BH, Lee JK, Kim TH and Kim W. (2007). Change of seed quality of Chinese milk vetch (Astragalus sinicus L.) during seed developmental stages. Korean J Crop Sci., 52:363–369.
  11. Okubo K, Hori N, Matoba R, Niiyama T, Fukushima A, Kojima Y and Matsubara K. (1992). Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression. Nat Genet., 2:173–179.
  12. Parry MAJ, Reynolds M, Salvucci ME, Raines C, Andralojc PJ. (2011). Raising yield potential of wheat. II. Increasing photosynthetic capacity and efficiency. J Exp Bot., 62: 453–467.
  13. Quevillon, E., Silventoinen, V., Pillai, S., Harte, N., Mulder, N., Apweiler, R. and Lopez, R. (2005). InterProScan: Protein domains identifier. Nucleic Acids Res., 33: W116CW120.
  14. Sang Yoon Kim, Chang Hoon Lee, Jessie Gutierrez and Pil Joo Kim (2013). Contribution of winter cover crop amendments on global warming potential in rice paddy soil during cultivation. Plant Soil, 366:273–286.
  15. Sharkey TD. (1985). Photosynthesis in intact leaves of C3 plants physics, physiology and rate limitations. Bot Rev., 51:53–    105
  16. Stephen P. Mayfield, Bennoun P, and Rochaix JD. (1987). Expression of the nuclear encoded OEE1 protein is required for oxygen evolution and stability of photosystem II particles in Chlamydomonas reinhardtii. EMBO J, 6:313-318.
  17. Stephen P. Mayfield, Michele Schirmer-Rahire, Gerhard Frank, Herbert Zuber and Jean-David Rochaix (1989). Analysis of the genes of the OEE1 and OEE3 proteins of the photosystem II complex from Chlamydomonas reinhardti. Plant Mol Biol., 12: 683-693.
  18. Stephen P. Mayfield (1991). Over-expression of the oxygen-evolving enhancer 1 protein and its consequences on photosystem II accumulation. Planta, 185:105-110.
  19. Xu DQ. (2001). Progress in photosynthesis research: From molecular mechanisms to green revolution. Acta Phytophysiologica Sinica, 27( 2): 97-108.
  20. Yanshi Xia, Zhengxiang Ning, Guihua Bai (2012). Allelic Variations of a Light Harvesting Chlorophyll A/B Binding Protein Gene (Lhcb1) Associated with Agronomic Traits in Barley. PLoS ONE, 7(5): e37573. doi:10.1371/    journal.pone.0037573.
  21. Zhu XG, Long SP, Ort DR. (2010). Improving Photosynthetic Efficiency for Greater Yield. Annu Rev Plant Biol., 61: 235–261.
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    volume 38 issue 1 (february 2015) : 1-8,   Doi: 10.5958/0976-0571.2015.00001.6

    An expressed sequence tags analysis for leaves of Chinese milk vetch (Astragalus sinicus)

    X
    X. Zhang*
    J
    J. Wang
    K
    K. Cao
    C
    C. Xu
    W
    W. Cao
    1Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
    Cite article:- Zhang* X., Wang J., Cao K., Xu C., Cao W. (2025). An expressed sequence tags analysis for leaves of Chinese milk vetch (Astragalus sinicus). Legume Research. 38(1): 1-8. doi: 10.5958/0976-0571.2015.00001.6.

    ABSTRACT

    Chinese milk vetch (Astragalus sinicus) is one of the most important green manure crops in temperate zone countries. To identify the genes involved in plant growth, a cDNA library was constructed from the mature leaves of Chinese milk vetch, and expressed sequence tag analysis was performed. Sequencing of the cDNA clones generated 1,027 high-quality expressed sequence tags clustered total 693 unigenes which included 110 Contigs and 583 Singlets. A total of 198 unigenes were most similar to Medicago truncatula genes. Genes related to photosynthesis were highly expressed in our library. The most abundant gene which had 42 clones was annotated as Ribulose bisphosphate carboxylase small chain with the function of carbon utilization by fixation of carbon dioxide. The results suggested that this high-quality library could be a good resource for understanding molecular events of Chinese milk vetch leaf growth, and the full-length clones could be used for crop improvement studies in the future.

    REFERENCES

    1. Adams, M. D., Kelley, J. M. Gocayne, J. D. Dubnick M. and Polymeropoulos M.H. (1991). Complementary DNA sequencing: expressed sequence tags and human genome project. Science, 252: 1651–1656.
    2. Amanda P. Cavanagh David S. and Kubien (2014). Can phenotypic plasticity in Rubisco performance contribute to photosynthetic acclimation? Photosynth Res., 119:203–214.
    3. Bellafiore S, Barneche F, Peltier G and Rochaix JD. (2005). State transitions and light adaptation require chloroplast thylakoid protein kinase STN7. Nature, 433: 892–895.
    4. Bouck AMY, Vision T. (2007). The molecular ecologist’s guide to expressed sequence tags. Mol Ecol., 16:907–924.
    5. Elfstrand S, Hedlund K, Martensson A. (2007). Soil enzyme activities, microbial community composition and function after 47 years of continuous green manuring. Appl Soil Ecol., 35:610–621.
    6. Farquhar GD, Sv Caemmerer and Berry JA. (1980). A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species. Planta, 149:78–90.
    7. Firn R and Jones C. (2009). A Darwinian view of metabolism: molecular properties determine fitness. J Exp Bot., 60:719–    726.
    8. Kim SY, Lee BJ, Kim JH, Oh SH, Hwang WH, Hwang DY, Ahn JW, Oh BJ and Ku YC. (2007). The timing for incorporating Chinese milk vetch plant into soil for natural reseeding in the southern part of Korean peninsula. Korean J Crop Sci., 52:127.
    9. Mary E. Knight, John A. Ray and Wolfgang Schuch (1992). Isolation of a gene from maize encoding a chlorophyll a/b-    binding protein. Plant Mol Biol., 19: 533-536.
    10. Na CS, Lee YH, Hong SH, Jang CS, Kang BH, Lee JK, Kim TH and Kim W. (2007). Change of seed quality of Chinese milk vetch (Astragalus sinicus L.) during seed developmental stages. Korean J Crop Sci., 52:363–369.
    11. Okubo K, Hori N, Matoba R, Niiyama T, Fukushima A, Kojima Y and Matsubara K. (1992). Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression. Nat Genet., 2:173–179.
    12. Parry MAJ, Reynolds M, Salvucci ME, Raines C, Andralojc PJ. (2011). Raising yield potential of wheat. II. Increasing photosynthetic capacity and efficiency. J Exp Bot., 62: 453–467.
    13. Quevillon, E., Silventoinen, V., Pillai, S., Harte, N., Mulder, N., Apweiler, R. and Lopez, R. (2005). InterProScan: Protein domains identifier. Nucleic Acids Res., 33: W116CW120.
    14. Sang Yoon Kim, Chang Hoon Lee, Jessie Gutierrez and Pil Joo Kim (2013). Contribution of winter cover crop amendments on global warming potential in rice paddy soil during cultivation. Plant Soil, 366:273–286.
    15. Sharkey TD. (1985). Photosynthesis in intact leaves of C3 plants physics, physiology and rate limitations. Bot Rev., 51:53–    105
    16. Stephen P. Mayfield, Bennoun P, and Rochaix JD. (1987). Expression of the nuclear encoded OEE1 protein is required for oxygen evolution and stability of photosystem II particles in Chlamydomonas reinhardtii. EMBO J, 6:313-318.
    17. Stephen P. Mayfield, Michele Schirmer-Rahire, Gerhard Frank, Herbert Zuber and Jean-David Rochaix (1989). Analysis of the genes of the OEE1 and OEE3 proteins of the photosystem II complex from Chlamydomonas reinhardti. Plant Mol Biol., 12: 683-693.
    18. Stephen P. Mayfield (1991). Over-expression of the oxygen-evolving enhancer 1 protein and its consequences on photosystem II accumulation. Planta, 185:105-110.
    19. Xu DQ. (2001). Progress in photosynthesis research: From molecular mechanisms to green revolution. Acta Phytophysiologica Sinica, 27( 2): 97-108.
    20. Yanshi Xia, Zhengxiang Ning, Guihua Bai (2012). Allelic Variations of a Light Harvesting Chlorophyll A/B Binding Protein Gene (Lhcb1) Associated with Agronomic Traits in Barley. PLoS ONE, 7(5): e37573. doi:10.1371/    journal.pone.0037573.
    21. Zhu XG, Long SP, Ort DR. (2010). Improving Photosynthetic Efficiency for Greater Yield. Annu Rev Plant Biol., 61: 235–261.
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