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Current IssueEditorial BoardOnline First ArticlesArchive

volume 37 issue 5 (october 2014) : 515-519,   Doi: 10.5958/0976-0571.2014.00668.7

EFFECT OF 28-HOMOBRASSINOLIDE ON THE DROUGHT INDUCED CHANGES IN THE SEEDS OF VIGNA RADIATA

M
Mohammed Nasser Alyemeni*
S
Sarah Mohammed Al-Quwaiz
1Department of Botany and Microbiology, King Saud University, Riyadh-11451, Saudia Arabia
Cite article:- Alyemeni* Nasser Mohammed, Al-Quwaiz Mohammed Sarah (2025). EFFECT OF 28-HOMOBRASSINOLIDE ON THE DROUGHT INDUCED CHANGES IN THE SEEDS OF VIGNA RADIATA. Legume Research. 37(5): 515-519. doi: 10.5958/0976-0571.2014.00668.7.

ABSTRACT

A study was undertaken to examine the different levels of drought stress during early seed germination and to explore the response of 28-homobrassinolide (HBL) as potent stress alleviator. Sterilized seeds of Vigna radiata (T-44 and PDM-139) were soaked in distilled water for 24 h then placed in 12 sets of 3 petri dishes representing various treatments. 7 days old seedlings were exposed  to drought stress equivalent to -6 bar (DS I) and – 12 bar using PEG-6000 and at 14 days stage seedlings exposed to 10-6, 10-8, 10-10 M of HBL. At 15 days stage, seedlings were harvested to evaluate the percent germination, proline, carbohydrate, nitrogen, phosphorus and potassium content. The results clearly indicated that seed germination, carbohydrate, nitrogen, phosphorus and potassium decreased in response to drought stress whereas, accumulation of proline increased. HBL increased proline accumulation significantly in the presence and absence of drought stress. It is concluded that drought stress (DSI ) deteriorates the metabolism of Vigna radiata by decreasing mineral-nutrient status and per cent germination more effectively in PDM-139 over T-44. However, HBL played pivotal role in maintaining the homeostasis of mineral-nutrient status of Vigna radiata and overcome drought stress through higher accumulation of proline.

REFERENCES

  1. Ali, B., Hayat, S., Hasan, S.A., and Ahmad, A. (2008) A comparative effect of IAA and 4-Cl-IAA on growth, nodulation and nitrogen fixation in Vigna radiata (L.) Wilczek. Acta. Physiol. Plant 30:35–41.
  2. Bajguz, A., (2000) Blockade of heavy metals accumulation in Chlorella vulgaris cells by 24-epibrassinolide. Plant Physiol. Biochem. 38:797–801.
  3. Bajguz, A., and Hayat, S. (2009) Effect of brassinosteroids on the plant responses to environmental stresses. Plant Physiol. Biochem. 47:1-8.
  4. Bandurska, H. (2001). Does proline accumulated in the leaves of water deficit stressed barley plants confine cell membrane injuries? II. Proline accumulation during hardening and its involvement in reducing membrane injuries in leaves subjected to severe osmotic stress. Acta. Physiol. Planta. 23:483–490.
  5. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Chem. 72: 248-254
  6. Fariduddin, Q., Yusuf, M., Ahmad, I., and Ahmad A. (2014) Brassinosteroids and their role in response of plants to abiotic stresses. Biol. Planta. 58: 9-17.
  7. Gomez, K.A. and Gomez, A.A. (1984). Statistical Procedures for Agricultural Research, John Wiley and Sons, New York.
  8. Hampson, C., and Simpson, G. (1990). Effects of temperature, salt, and osmotic potential on early growth of wheat (Triticum aestivum). I. Germination. Can. J. Bot. 68:524-528.
  9. Hartzendorf, T., and Rolletschek, H. (2001). Effect of NaCl salinity on amino acid and carbohydrate contents of Phragmites australis. Aqu. Bot. 69:195–208.
  10. Hayat, S., Hasan, S.A., Yusuf, M., Hayat, Q., and Ahmad, A. (2010) Effect of 28 homobrassinolide on photosynthesis, fluorescence and antioxidant system in the presence or absence of salinity and temperature in Vigna radiata. Environ. Exp. Bot. 69:105–112.
  11. Hopkins, W.G. (1995) Introduction to Plant Physiology. John Wiley & Sons, Inc. New York, USA
  12. Jain, M., Mathur, G., Koul, S., and Sarin, N. B. (2001). Ameliorative effect of proline on the salt stress induced lipid peroxidation in cell lines of groundnut (Arachis hypogea L.). Plant Cell Reports 20:463–468.
  13. Kagale, S., Divi, U.K., Krochko, J.E., Keller, W.A., and Krishna, P. (2007) Brassinosteroids confers tolerance in Arabidopsis thaliana and Brassica napus to a range of abiotic stresses. Planta 225:353-364.
  14. Marschner, H. (1995) Mineral nutrition of higher plants. 2nd Edition. Academic press, San Diego, USA p. 889.
  15. Mengel, K., and Kirkby, E. A. (2001) Principles of Plant Nutrition. 5th Edition, Kluwer Academic Publishers, Dordrecht.
  16. Pratap, V., and Sharma, Y.K. (2010). Impact of osmotic stress on seed germination and seedling growth in black gram (Phaseolus mungo). J Environ. Biol. 31:721-726.
  17. Sasse, J.M. (2003) Physiological actions of Brassinosteroids: an update. J Plant Growth Regul. 22:276–288.
  18. Somerville, C., and Briscoe, J. (2001) Genetic engineering and water. Science 292: 2217
  19. Vyas, S.P., Kathju, S., Garg, B.K., and Lahiri, A.N. (1996). Activities of nitrate reductase and ammonia assimilating enzymes of moth bean under water stress. Sci. Cult. 62:213-214.
  20. Waraich, E.A., Ahmad, R., Saifullah., Ashraf, M.Y., and Ehsanullah. (2011) Role of mineral nutrition in alleviation of drought stress in plants. Aus. J Crop Sci. 5:764-777.
  21. Yordanov, I., Velikova, V., and Tsonev, T. (2003). Plant responses to drought and stress tolerance. Bulg. J. Plant Physiol. Special issue 187-206.
  22. Yusuf, M., Fariduddin, Q., and Ahmad, A. (2012) 24-Epibrassinolide modulates growth, nodulation, antioxidant system, and osmolyte in tolerant and sensitive varieties of Vigna radiata under different levels of nickel: A shotgun approach. Plant Physiol. Biochem. 57:143-153.
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    volume 37 issue 5 (october 2014) : 515-519,   Doi: 10.5958/0976-0571.2014.00668.7

    EFFECT OF 28-HOMOBRASSINOLIDE ON THE DROUGHT INDUCED CHANGES IN THE SEEDS OF VIGNA RADIATA

    M
    Mohammed Nasser Alyemeni*
    S
    Sarah Mohammed Al-Quwaiz
    1Department of Botany and Microbiology, King Saud University, Riyadh-11451, Saudia Arabia
    Cite article:- Alyemeni* Nasser Mohammed, Al-Quwaiz Mohammed Sarah (2025). EFFECT OF 28-HOMOBRASSINOLIDE ON THE DROUGHT INDUCED CHANGES IN THE SEEDS OF VIGNA RADIATA. Legume Research. 37(5): 515-519. doi: 10.5958/0976-0571.2014.00668.7.

    ABSTRACT

    A study was undertaken to examine the different levels of drought stress during early seed germination and to explore the response of 28-homobrassinolide (HBL) as potent stress alleviator. Sterilized seeds of Vigna radiata (T-44 and PDM-139) were soaked in distilled water for 24 h then placed in 12 sets of 3 petri dishes representing various treatments. 7 days old seedlings were exposed  to drought stress equivalent to -6 bar (DS I) and – 12 bar using PEG-6000 and at 14 days stage seedlings exposed to 10-6, 10-8, 10-10 M of HBL. At 15 days stage, seedlings were harvested to evaluate the percent germination, proline, carbohydrate, nitrogen, phosphorus and potassium content. The results clearly indicated that seed germination, carbohydrate, nitrogen, phosphorus and potassium decreased in response to drought stress whereas, accumulation of proline increased. HBL increased proline accumulation significantly in the presence and absence of drought stress. It is concluded that drought stress (DSI ) deteriorates the metabolism of Vigna radiata by decreasing mineral-nutrient status and per cent germination more effectively in PDM-139 over T-44. However, HBL played pivotal role in maintaining the homeostasis of mineral-nutrient status of Vigna radiata and overcome drought stress through higher accumulation of proline.

    REFERENCES

    1. Ali, B., Hayat, S., Hasan, S.A., and Ahmad, A. (2008) A comparative effect of IAA and 4-Cl-IAA on growth, nodulation and nitrogen fixation in Vigna radiata (L.) Wilczek. Acta. Physiol. Plant 30:35–41.
    2. Bajguz, A., (2000) Blockade of heavy metals accumulation in Chlorella vulgaris cells by 24-epibrassinolide. Plant Physiol. Biochem. 38:797–801.
    3. Bajguz, A., and Hayat, S. (2009) Effect of brassinosteroids on the plant responses to environmental stresses. Plant Physiol. Biochem. 47:1-8.
    4. Bandurska, H. (2001). Does proline accumulated in the leaves of water deficit stressed barley plants confine cell membrane injuries? II. Proline accumulation during hardening and its involvement in reducing membrane injuries in leaves subjected to severe osmotic stress. Acta. Physiol. Planta. 23:483–490.
    5. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Chem. 72: 248-254
    6. Fariduddin, Q., Yusuf, M., Ahmad, I., and Ahmad A. (2014) Brassinosteroids and their role in response of plants to abiotic stresses. Biol. Planta. 58: 9-17.
    7. Gomez, K.A. and Gomez, A.A. (1984). Statistical Procedures for Agricultural Research, John Wiley and Sons, New York.
    8. Hampson, C., and Simpson, G. (1990). Effects of temperature, salt, and osmotic potential on early growth of wheat (Triticum aestivum). I. Germination. Can. J. Bot. 68:524-528.
    9. Hartzendorf, T., and Rolletschek, H. (2001). Effect of NaCl salinity on amino acid and carbohydrate contents of Phragmites australis. Aqu. Bot. 69:195–208.
    10. Hayat, S., Hasan, S.A., Yusuf, M., Hayat, Q., and Ahmad, A. (2010) Effect of 28 homobrassinolide on photosynthesis, fluorescence and antioxidant system in the presence or absence of salinity and temperature in Vigna radiata. Environ. Exp. Bot. 69:105–112.
    11. Hopkins, W.G. (1995) Introduction to Plant Physiology. John Wiley & Sons, Inc. New York, USA
    12. Jain, M., Mathur, G., Koul, S., and Sarin, N. B. (2001). Ameliorative effect of proline on the salt stress induced lipid peroxidation in cell lines of groundnut (Arachis hypogea L.). Plant Cell Reports 20:463–468.
    13. Kagale, S., Divi, U.K., Krochko, J.E., Keller, W.A., and Krishna, P. (2007) Brassinosteroids confers tolerance in Arabidopsis thaliana and Brassica napus to a range of abiotic stresses. Planta 225:353-364.
    14. Marschner, H. (1995) Mineral nutrition of higher plants. 2nd Edition. Academic press, San Diego, USA p. 889.
    15. Mengel, K., and Kirkby, E. A. (2001) Principles of Plant Nutrition. 5th Edition, Kluwer Academic Publishers, Dordrecht.
    16. Pratap, V., and Sharma, Y.K. (2010). Impact of osmotic stress on seed germination and seedling growth in black gram (Phaseolus mungo). J Environ. Biol. 31:721-726.
    17. Sasse, J.M. (2003) Physiological actions of Brassinosteroids: an update. J Plant Growth Regul. 22:276–288.
    18. Somerville, C., and Briscoe, J. (2001) Genetic engineering and water. Science 292: 2217
    19. Vyas, S.P., Kathju, S., Garg, B.K., and Lahiri, A.N. (1996). Activities of nitrate reductase and ammonia assimilating enzymes of moth bean under water stress. Sci. Cult. 62:213-214.
    20. Waraich, E.A., Ahmad, R., Saifullah., Ashraf, M.Y., and Ehsanullah. (2011) Role of mineral nutrition in alleviation of drought stress in plants. Aus. J Crop Sci. 5:764-777.
    21. Yordanov, I., Velikova, V., and Tsonev, T. (2003). Plant responses to drought and stress tolerance. Bulg. J. Plant Physiol. Special issue 187-206.
    22. Yusuf, M., Fariduddin, Q., and Ahmad, A. (2012) 24-Epibrassinolide modulates growth, nodulation, antioxidant system, and osmolyte in tolerant and sensitive varieties of Vigna radiata under different levels of nickel: A shotgun approach. Plant Physiol. Biochem. 57:143-153.
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