Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 37 issue 1 (february 2014) : 68-71,   Doi: 10.5958/j.0976-0571.37.1.010

DO BRASSINOSTEROIDS AMELIORATE FREEZING STRESS IN CICER ARIETINUM

S
Shamsul Hayat*
M
Mohammed Nasser Alyemeni
S
Sweta Yadav
1Department of Botany & Microbiology, King Saud University, Riyadh, Saudi Arabia
Cite article:- Hayat* Shamsul, Alyemeni Nasser Mohammed, Yadav Sweta (2025). DO BRASSINOSTEROIDS AMELIORATE FREEZING STRESS IN CICER ARIETINUM. Legume Research. 37(1): 68-71. doi: 10.5958/j.0976-0571.37.1.010.

ABSTRACT

An experiment was conducted to examine the extent of low-temperature stress during early seed germination and to investigate the mitigating effects of brassinosteroids. Sterilized Cicer arietinum seeds were soaked in distilled water for 24 h then placed in three sets of cotton-lined petri dishes. These three sets of seeds were exposed to different temperatures: 25°C (control), 0°C and -10°C. Another experiment was conducted using the same set of conditions but included a 24 h soaking step in 28-homobrassinolide (HBL) under controlled conditions. The seedlings were evaluated after 72 h. The results clearly indicated that seed germination, the activities of nitrate reductase and carbonic anhydrase, the accumulated levels of proline and protein decreased in response to low-temperature stress. HBL partially ameliorated the stress at 0°C but failed to overcome the -10°C stress. Based on our observations, it could be concluded that brassinosteroids could partially overcome the stress generated by 0°C during the seed germination stage but could not overcome the stress generated by -10°C.

REFERENCES

  1. Bajguz, A. and Hayat, S. (2009). Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiol Biochem. 47(1):1-8
  2. Bingkui, W. and Guangwen, Z. (1993). Effect of epibrassinolide on the resistance of rice seedlings to chilling injury. J. Plant Physiol. Mol. Biol. 19(1):38-42
  3. Feng, Z.Z., Guo, A. and Feng, A. (2003). Amelioration of chilling stress by triadimefon in cucumber seedlings Plant Growth Reg. 39:277–283.
  4. Gomez, K.A. and Gomez, A.A. (1984). Statistical Procedures for Agricultural Research, John Wiley and Sons, New York.
  5. Hayat, S., Hasan, S.A., Yusuf, M., Hayat, Q., and Ahmad, A. (2010) Effect of 28-homobrassinolide on photosynthesis, fluorescence and antioxidant system in the presence or absence of salinity and temperature in Vigna radiata, Environ. Exp. Bot. 69: 105–112.
  6. Hopkins, W.J. (1995). Physiology of Plant under Stress. In: Introduction to Plant Physiology. John Wiley & Sons Inc. New York. p. 438.
  7. Ihsanullah, D.and Ozgur, T. (2013). Effects of gypsum and brassinolide on soil properties and berseem (trifolium alexandrinum L.) growth, yield and chemical composition grown on saline soil, Legume. Res. 36: 306-311.
  8. Kamuro, Y. and Takatsuto, S. (1991). Capability for the problems of practical uses of brassinosteroids. p. 292-297. In Brassinosteroids: Chemistry, Bioactivity and Application. [H.G. Cutler, T. Yokota and G. Adam, (eds.)] ACS Symposium, Series 474, American Chemical Society, Washington, DC.
  9. Lal, S., Bagdi D.L., Kakralya, B.L., Jat, M.L. and Sharma, P.C. (2013). Role of brassinolide in alleviating the adverse effect of drought stress on physiology, growth and yield of green gram (Vigna radiata L.) genotypes. Legume. Res. 36: 359-363.
  10. Mai, Y., Lin, S., Zeng, X. and Ran, R. (1989). Effect of brassinolide on nitrate reductase activity in rice seedlings. Plant Physiol. Comm. 2:50-52.
  11. Mangrich, M.E. and Saltveit, M.E. (2000). Effect of chilling, heat shock, and vigor on the growth of cucumber (Cucumis sativus) radicles. Physiol. Plant. 109: 137–142.
  12. Pell, E.J. and Dann, M.S. (1991). Multiple stress and plant senescence. p. 189–284. In: Integrated Response of Plants to Stress. H.A. Mooney, W.E. Winner and E.J. Pell (eds.), Academic Press, San Diego.
  13. Saltveit, M.E. (2000). Discovery of chilling Injury. Vol.3: In: Discoveries in plant biology. S.D. Kung and S.F. Yang (eds.), World Scientific Publications, Singaporep. 423–448.
  14. Salvucci, M.E. and Crafts-Brander, S.J. (2004). Relationship between the heat tolerance of photosynthesis and the thermal stability of rubisco activase in plants from contrasting thermal environments. Plant Physiol. 134:1460-    1470.
  15. Saruyama, H. and Tanida, M. (1995). Effect of chilling on activated oxygen-scavenging enzymes in low temperature-    sensitive and -tolerant cultivars of rice (Oryza sativa L.). Plant Sci. 109:105–113.
  16. Singh, R., Singh, N.P., Datta, S., Yadav, I.S. and Singh, A.P. (2009). Agrobacterium-mediated transformation of chickpea using shoot meristem. Indian. J. Biotech. 8:78-84.
  17. Tikhomirova, E.V. (1985). Changes of nitrogen metabolism in millet at alleviated temperatures. Field Crops Res. 11:259-    264.
  18. Wehmeyer, N. and Vierling, E. (2000). The expression of small heat shock proteins in seeds responds to discrete development signals and suggests a general protective role in desiccation tolerance. Plant Physiol. 122:1099-1108.
  19. Yang, M.T., Chen, S.L. and Lincy, D. (2005). Chilling stress suppresses chloroplast development and nuclear gene expression in leaves of mungbean seedlings. Planta 221:374-385.
  20. Zhou, Y., Huang, L., Zhang, Y., Shi, K., Yu, J. and Nogués, S. (2007). Chill-induced decrease in capacity of RuBP carboxylation and associated H2O2 accumulation in cucumber leaves are alleviated by grafting onto fig leaf gourd. Ann. Bot. 100:839-848.
Published In
Legume Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 37 issue 1 (february 2014) : 68-71,   Doi: 10.5958/j.0976-0571.37.1.010

    DO BRASSINOSTEROIDS AMELIORATE FREEZING STRESS IN CICER ARIETINUM

    S
    Shamsul Hayat*
    M
    Mohammed Nasser Alyemeni
    S
    Sweta Yadav
    1Department of Botany & Microbiology, King Saud University, Riyadh, Saudi Arabia
    Cite article:- Hayat* Shamsul, Alyemeni Nasser Mohammed, Yadav Sweta (2025). DO BRASSINOSTEROIDS AMELIORATE FREEZING STRESS IN CICER ARIETINUM. Legume Research. 37(1): 68-71. doi: 10.5958/j.0976-0571.37.1.010.

    ABSTRACT

    An experiment was conducted to examine the extent of low-temperature stress during early seed germination and to investigate the mitigating effects of brassinosteroids. Sterilized Cicer arietinum seeds were soaked in distilled water for 24 h then placed in three sets of cotton-lined petri dishes. These three sets of seeds were exposed to different temperatures: 25°C (control), 0°C and -10°C. Another experiment was conducted using the same set of conditions but included a 24 h soaking step in 28-homobrassinolide (HBL) under controlled conditions. The seedlings were evaluated after 72 h. The results clearly indicated that seed germination, the activities of nitrate reductase and carbonic anhydrase, the accumulated levels of proline and protein decreased in response to low-temperature stress. HBL partially ameliorated the stress at 0°C but failed to overcome the -10°C stress. Based on our observations, it could be concluded that brassinosteroids could partially overcome the stress generated by 0°C during the seed germination stage but could not overcome the stress generated by -10°C.

    REFERENCES

    1. Bajguz, A. and Hayat, S. (2009). Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiol Biochem. 47(1):1-8
    2. Bingkui, W. and Guangwen, Z. (1993). Effect of epibrassinolide on the resistance of rice seedlings to chilling injury. J. Plant Physiol. Mol. Biol. 19(1):38-42
    3. Feng, Z.Z., Guo, A. and Feng, A. (2003). Amelioration of chilling stress by triadimefon in cucumber seedlings Plant Growth Reg. 39:277–283.
    4. Gomez, K.A. and Gomez, A.A. (1984). Statistical Procedures for Agricultural Research, John Wiley and Sons, New York.
    5. Hayat, S., Hasan, S.A., Yusuf, M., Hayat, Q., and Ahmad, A. (2010) Effect of 28-homobrassinolide on photosynthesis, fluorescence and antioxidant system in the presence or absence of salinity and temperature in Vigna radiata, Environ. Exp. Bot. 69: 105–112.
    6. Hopkins, W.J. (1995). Physiology of Plant under Stress. In: Introduction to Plant Physiology. John Wiley & Sons Inc. New York. p. 438.
    7. Ihsanullah, D.and Ozgur, T. (2013). Effects of gypsum and brassinolide on soil properties and berseem (trifolium alexandrinum L.) growth, yield and chemical composition grown on saline soil, Legume. Res. 36: 306-311.
    8. Kamuro, Y. and Takatsuto, S. (1991). Capability for the problems of practical uses of brassinosteroids. p. 292-297. In Brassinosteroids: Chemistry, Bioactivity and Application. [H.G. Cutler, T. Yokota and G. Adam, (eds.)] ACS Symposium, Series 474, American Chemical Society, Washington, DC.
    9. Lal, S., Bagdi D.L., Kakralya, B.L., Jat, M.L. and Sharma, P.C. (2013). Role of brassinolide in alleviating the adverse effect of drought stress on physiology, growth and yield of green gram (Vigna radiata L.) genotypes. Legume. Res. 36: 359-363.
    10. Mai, Y., Lin, S., Zeng, X. and Ran, R. (1989). Effect of brassinolide on nitrate reductase activity in rice seedlings. Plant Physiol. Comm. 2:50-52.
    11. Mangrich, M.E. and Saltveit, M.E. (2000). Effect of chilling, heat shock, and vigor on the growth of cucumber (Cucumis sativus) radicles. Physiol. Plant. 109: 137–142.
    12. Pell, E.J. and Dann, M.S. (1991). Multiple stress and plant senescence. p. 189–284. In: Integrated Response of Plants to Stress. H.A. Mooney, W.E. Winner and E.J. Pell (eds.), Academic Press, San Diego.
    13. Saltveit, M.E. (2000). Discovery of chilling Injury. Vol.3: In: Discoveries in plant biology. S.D. Kung and S.F. Yang (eds.), World Scientific Publications, Singaporep. 423–448.
    14. Salvucci, M.E. and Crafts-Brander, S.J. (2004). Relationship between the heat tolerance of photosynthesis and the thermal stability of rubisco activase in plants from contrasting thermal environments. Plant Physiol. 134:1460-    1470.
    15. Saruyama, H. and Tanida, M. (1995). Effect of chilling on activated oxygen-scavenging enzymes in low temperature-    sensitive and -tolerant cultivars of rice (Oryza sativa L.). Plant Sci. 109:105–113.
    16. Singh, R., Singh, N.P., Datta, S., Yadav, I.S. and Singh, A.P. (2009). Agrobacterium-mediated transformation of chickpea using shoot meristem. Indian. J. Biotech. 8:78-84.
    17. Tikhomirova, E.V. (1985). Changes of nitrogen metabolism in millet at alleviated temperatures. Field Crops Res. 11:259-    264.
    18. Wehmeyer, N. and Vierling, E. (2000). The expression of small heat shock proteins in seeds responds to discrete development signals and suggests a general protective role in desiccation tolerance. Plant Physiol. 122:1099-1108.
    19. Yang, M.T., Chen, S.L. and Lincy, D. (2005). Chilling stress suppresses chloroplast development and nuclear gene expression in leaves of mungbean seedlings. Planta 221:374-385.
    20. Zhou, Y., Huang, L., Zhang, Y., Shi, K., Yu, J. and Nogués, S. (2007). Chill-induced decrease in capacity of RuBP carboxylation and associated H2O2 accumulation in cucumber leaves are alleviated by grafting onto fig leaf gourd. Ann. Bot. 100:839-848.
    In this Article
      Contact us
      Follow us
      Published In
      Legume Research

      Editorial Board

      View all (0)