Article Id: ARCC876 | Page : 242 - 250
M. Zia-ul-haq1, M. Nisar2, M.R. Shah3, M. Akhter4, M. Qayum5, S. Ahmad6, Shakir A. Shahid7 and Mirza Hasanuzzaman8*
Address : Department of Pharmacognosy University of Karachi, Karachi - 752 70, Pakistan


Legumes are excellent sources of protein, carbohydrates, dietary fibre, lipids, vitamins, carotenoids, macronutrients, a variety of micronutrients and phytochemicals. In a study carried in the University of Karachi in 2009, ethanolic extracts of seeds of eight legumes namely pea (Pisum sativum), desi and kabuli chickpea (Cicer arietinum L), cowpea (Vigna unguiculata (L) Walp.), mash bean (Phaseolus mungo), mung bean (Vigna radiate L. wilczek), soybean (Glycine max) and lentil (Lens culinaris Medik.) were tested for different biological activities such as insecticidal activity, phytotoxic activity, wormicidal, anthelmintic activity as well as lethality bioassay. Most extracts (desi chickpea, cowpea, mash bean, mung bean, soybean and lentil) showed moderate activity at higher concentration of 1000 ìg ml-1 and low or no activity at low doses (100 and 10µg ml-1). In vitro results showed that ethanolic extract of these plants caused appreciable mortality of second stage juveniles of Cephalobus litoralis and Helicotylenchus indicus. Higher concentrations used (2 % and 1 %) were found more effective and produced significant results as compared to 0.5 % of extracts. The mortality rate increased with increasing exposure time and was highest after 48 hours.


Antimicrobial activity; biological control; grain legumes; phytotoxicity.


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