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ELECTROPHORETIC STUDIES ON SEED PROTEIN PROFILE OF MUNGBEAN

Article Id: ARCC4025
Citation :- ELECTROPHORETIC STUDIES ON SEED PROTEIN PROFILE OF MUNGBEAN.Legume Research.2001.(24)
Naik B.S., Kole C.
Address : Laboratory of Molecular Biology and Biotechnology, Orrisa University of Agriculture and Technology, Bhubaneswar - 751 003, India

Abstract

SDS-polyacrylamide gel electrophoresis of total seed proteins of 37 mungbean genotypes evidenced for the presence of 23 polypeptide bands dispersed over five zones, A to E within a molecular weight range of 17.4 to 75.0 kD. The banding patterns exhibited polymorphism for the presence of a band of 62.4 kD, B1 or two bands present jointly (61.0 and 58.2 kD), B2+ B3 indicating absence of gene(s) involved in post-translational dissociation in the genotypes without these two bands. Another band of 30.2 kD, D2(D2α) had a high molecular weight variant (D2β) of 31.3 kD. This is the first report on a detailed electrophoretic banding pattern of mungbean based on a large number of Indian cultivars.

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