MORPHOGENESIS AND PLANTLET REGENERATION FROM SOYBEAN (GLYCINE MAX L. MERRILL) LEAF DISCS INFLUENCED BY GENOTYPES AND PLANT GROWTH REGULATORS

T
Tripathi Manoj
T
Tiwari Sharad
1 Plant Biotechnology Laboratory, Department of Plant Breeding and Genetics,J.N. Agricultural University, Jabalpur - 482 004, India
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  • First Online |

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Cite article:- Manoj Tripathi, Sharad Tiwari (2026). MORPHOGENESIS AND PLANTLET REGENERATION FROM SOYBEAN (GLYCINE MAX L. MERRILL) LEAF DISCS INFLUENCED BY GENOTYPES AND PLANT GROWTH REGULATORS. Legume Research. 27(2): . doi: .
Leaf discs of eleven genotypes of Glycine max were cultured on three different fortifications of MS medium i.e. MS30D (30.0 mg P 2,4-D), MS10PB (10.0 mg l−1 PCPA+ 0.5 mg P BAP) and MS3PB (3.0 mg P PCPA+ 0.5 mg l−1 BAP). High significant differences in the response of genotypes, culture media and genotype x medium interactions were experimented for callus initiation {Qrmation of morphogenic and shoot forming calli and plantlet regeneration from leaf discs. Results designated combination of an auxin at a higher level and BAP at a lower level most responsive for all culture phases of soybean leaf discs.

    MORPHOGENESIS AND PLANTLET REGENERATION FROM SOYBEAN (GLYCINE MAX L. MERRILL) LEAF DISCS INFLUENCED BY GENOTYPES AND PLANT GROWTH REGULATORS

    T
    Tripathi Manoj
    T
    Tiwari Sharad
    1 Plant Biotechnology Laboratory, Department of Plant Breeding and Genetics,J.N. Agricultural University, Jabalpur - 482 004, India
    • Submitted|

    • First Online |

    • doi

    Cite article:- Manoj Tripathi, Sharad Tiwari (2026). MORPHOGENESIS AND PLANTLET REGENERATION FROM SOYBEAN (GLYCINE MAX L. MERRILL) LEAF DISCS INFLUENCED BY GENOTYPES AND PLANT GROWTH REGULATORS. Legume Research. 27(2): . doi: .
    Leaf discs of eleven genotypes of Glycine max were cultured on three different fortifications of MS medium i.e. MS30D (30.0 mg P 2,4-D), MS10PB (10.0 mg l−1 PCPA+ 0.5 mg P BAP) and MS3PB (3.0 mg P PCPA+ 0.5 mg l−1 BAP). High significant differences in the response of genotypes, culture media and genotype x medium interactions were experimented for callus initiation {Qrmation of morphogenic and shoot forming calli and plantlet regeneration from leaf discs. Results designated combination of an auxin at a higher level and BAP at a lower level most responsive for all culture phases of soybean leaf discs.
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