CHROMOSOMAL VARIATIONS IN IMMATURE EMBRYO DERIVED CALLI OF BLACKGRAM (VIGNA MUNGO L.) HEPPER

Article Id: ARCC3704 | Page : 42 - 45
Citation :- CHROMOSOMAL VARIATIONS IN IMMATURE EMBRYO DERIVED CALLI OF BLACKGRAM (VIGNA MUNGO L.) HEPPER.Legume Research-An International Journal.2005.(28):42 - 45
V.L. Mathur and Sandeep Kaushik
Address : Department of Plant Breec!ing and Genetics. Rajasthan College of Agriculture. Udaipur· 313 001. India

Abstract

A study on the variation in chromosome number of the callus cells derived from immature embryo explant of Vigna mungo L. Hepper was undertaken. The chromosome number showed a wide range of variation, however the diploid cells were dominated in all the cultures. The frequency of tetraploid cells was higher than higher ploidy level, it increased with increase in the concentration of phytohormones. Plant regeneration was achieved after 30 days of inoculation from immature embryo explant cultured on MS media supplemented with 1 mg/1 2,4-D to 0.1 mg/kinetin.

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