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Current IssueEditorial BoardOnline First ArticlesArchive

volume 36 issue 1 (february 2013) : 49-55

EXTRACTION AND GC STUDY ON SWAINSONINE FROM LOCOWEED ASTRAGALUS STRICTUS IN TIBET

B
Baocheng Hao
X
Xianpeng Yang
X
Xuehong Wang
Y
Yonghao Hu
J
Jianping Liang
1Key Laboratory of New Animal Drug Project,Gansu province/ Key Laboratory of Veterinary Pharmaceutics Discovery ,Agricultural Ministry/ Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS,Lanzhou 730050

  • Submitted|

  • First Online |

  • doi

Cite article:- Hao Baocheng, Yang Xianpeng, Wang Xuehong, Hu Yonghao, Liang Jianping (2025). EXTRACTION AND GC STUDY ON SWAINSONINE FROM LOCOWEED ASTRAGALUS STRICTUS IN TIBET. Legume Research. 36(1): 49-55. doi: .

ABSTRACT

Locoweed is a poisonous plantÿwhich is wildly distributed in most area of the worldÿand cause livestock poisoning or death with serious economic loss. The Astragalusstrictus belongs to a species of locoweed .It is mainly distributed in Tibet of China and is a serious hazard to the local livestock industry. The objective of this study is extracting and purifying condition of Swainsonine fromAstragalusstrictuswith the microwave-assisted extraction method. An optimum extracting technology of SW fromAstragalusstrictuswas investigated through the orthogonal experiment under the microwave assistance conditions, then the content of swainsonine was analyzed with gas chromatographyÿGC method. The optimized extraction conditions were as follow: at room temperature, the time of using the petroleum ether ultrasonic to make the Swainsonine degreased with ultrasonic was 1h, and then use the method of extraction with microwave-assisted, solid-liquid ratio (M : V) 1:10, microwave frequencies 280W , extraction time 20 s. Under the above conditions, the extraction percentage of the swainsonine was 0.009264%. Extracting swainsonine with microwave-assisted extraction method has characteristics of rapid, efficient, convenient and low energy consumption. It is beneficial for producing swainsonine fromAstragalusstrictus.

REFERENCES

  1. Colegate,S.M., Dorling,P.R. and Huxtable, C.R. (1979). A spectroscopic investigation of swainsonine: an a mannosidase inhibitor isolated from Swainsinacanecens. Aust J Chem. 32:2257-2264.
  2. Dennis, J.W., Koch, K.,Yousefi, S. and Vanderelst, I. (1990).Growth inhibition of human melanoma tumor xenografts in athymic nude mice by swainsonine. Cancer Res. 50(6):1867-1872.
  3. Guo,X.W., Zhang,F.C. and Lin,S.Y. (1993). The application of ultrasound technology in the extraction of herbal ingredients. Chinese Herbal Medicines.24(10): 548-550.
  4. James,L.F. and Hartley,W.J. (1977). Effect s of milk from animals feed locoweed on kittens, calves and lambs. Am J Vet Res. 38:1263-1265.
  5. Li, J.K. (2003) .Research and prospect of locoweed of China. Agric. Sci. China.36(9):1091-1099.
  6. Liu,Z.Y., Zhao,B.Y., Sun,L.S,,Wan,X.P. and Huo, X.H. (2008). Research advances in swainsonine resources and its detection methods. Chinese Vet. Sci. 38(02):175-180.
  7. Lu,H.(WangÿJ.J., Sun,L.S.)Zhao,B.Y., Wang and Z.X., Ma,R. (2009). Progress on Antineoplastic Activity of Swainsonine. Prog. Vet. Medicine. 30(9) :87-90.
  8. Song,Y.M., Wang,J.H., Wang,A.H., Wang,Y. and Yu,Y.T. (2010).The determination of swainsonine in rabbit serum by gas chromatography. Chinese Journal of Veterinary Science. 30(3): 402-405.
  9. Tong, D.W. (2000).Studies on synthesis of swainsonine of swainsonine - BSA and its immunogenicity.Northwest A & F University, PhD thesis of Materials physics and chemistry professional.
  10. Wang,K., MO,C.H., Zhao,B.Y. and Cao,G.R.. (1999).”Jifang No. E” prevention sheep Poisoning of Gansu oxytropis.Anim. Husb. Vet. Medicine. 3:31-32.
  11. Wang,S., Chen,G.Y., Hu,J.J. and Ma,C.H. (2011). Determination of Swainsonine Content in Oxytrpisglabra DC in South Xinjiang by Internal Standard Gas Chromatography. Xinjiang Agric. Sci.48(4):728-733.
  12. Wu, D., Liang, B., Feng, Z.G. and Shi,Y.P. (2003). Research progress of swainsonine. Chinese Traditional Vet. Medicine.2:42-45.
  13. Yang, M. Q.,Cao (G.R.,Li), S.J. (1998). Extraction of swainsonine of the beans Rhizoctonia. J. Northwestern Agric. Univ. 26(4): 87-90.
  14. Zhao B.Y., Fan, Y.Y. Fan, Z.F. Dong, Q. and Cao, G.R. (2006). Hazards of locoweed in the west grassland of China and the control of animals loco-toxicosis. Grass-Feeding Livestock.130:12–15.
  15. Zhao, B.Y.. (2007).The Northwest Agricultural developed grassland poisonous locoweed antidote.1:66.
  16. Zhao,J.,Tong, D.W., Shui, Y.Y., Zhao, B.Y., Liu, W.M. and Li,L. (2006).Compariso-n of Swainsonine-Extracting Technologies from Oxytropiskansuensis Bunge.ActaBotanicaBoreali-OccidentaliaSinica.26(7):1459-1463.
  17. Zeng,L. and Xia,Z.N. (2002).The improvement and influence of ultrasonic and microwave irradiation on the extraction of traditional Chinese medicine. Chemical Res. Application. 14(3): 245-249.
  18. Zhao, X.H., He,X. and Wang, J.H. (2011).Determination of Swainsonine of Astragalusvariabilis Bunge by Gas Chromatography with Solvent Extraction. Acta Agriculturae Boreali-Occidentalis Sinica. 20(6):71-74
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    volume 36 issue 1 (february 2013) : 49-55

    EXTRACTION AND GC STUDY ON SWAINSONINE FROM LOCOWEED ASTRAGALUS STRICTUS IN TIBET

    B
    Baocheng Hao
    X
    Xianpeng Yang
    X
    Xuehong Wang
    Y
    Yonghao Hu
    J
    Jianping Liang
    1Key Laboratory of New Animal Drug Project,Gansu province/ Key Laboratory of Veterinary Pharmaceutics Discovery ,Agricultural Ministry/ Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS,Lanzhou 730050

    • Submitted|

    • First Online |

    • doi

    Cite article:- Hao Baocheng, Yang Xianpeng, Wang Xuehong, Hu Yonghao, Liang Jianping (2025). EXTRACTION AND GC STUDY ON SWAINSONINE FROM LOCOWEED ASTRAGALUS STRICTUS IN TIBET. Legume Research. 36(1): 49-55. doi: .

    ABSTRACT

    Locoweed is a poisonous plantÿwhich is wildly distributed in most area of the worldÿand cause livestock poisoning or death with serious economic loss. The Astragalusstrictus belongs to a species of locoweed .It is mainly distributed in Tibet of China and is a serious hazard to the local livestock industry. The objective of this study is extracting and purifying condition of Swainsonine fromAstragalusstrictuswith the microwave-assisted extraction method. An optimum extracting technology of SW fromAstragalusstrictuswas investigated through the orthogonal experiment under the microwave assistance conditions, then the content of swainsonine was analyzed with gas chromatographyÿGC method. The optimized extraction conditions were as follow: at room temperature, the time of using the petroleum ether ultrasonic to make the Swainsonine degreased with ultrasonic was 1h, and then use the method of extraction with microwave-assisted, solid-liquid ratio (M : V) 1:10, microwave frequencies 280W , extraction time 20 s. Under the above conditions, the extraction percentage of the swainsonine was 0.009264%. Extracting swainsonine with microwave-assisted extraction method has characteristics of rapid, efficient, convenient and low energy consumption. It is beneficial for producing swainsonine fromAstragalusstrictus.

    REFERENCES

    1. Colegate,S.M., Dorling,P.R. and Huxtable, C.R. (1979). A spectroscopic investigation of swainsonine: an a mannosidase inhibitor isolated from Swainsinacanecens. Aust J Chem. 32:2257-2264.
    2. Dennis, J.W., Koch, K.,Yousefi, S. and Vanderelst, I. (1990).Growth inhibition of human melanoma tumor xenografts in athymic nude mice by swainsonine. Cancer Res. 50(6):1867-1872.
    3. Guo,X.W., Zhang,F.C. and Lin,S.Y. (1993). The application of ultrasound technology in the extraction of herbal ingredients. Chinese Herbal Medicines.24(10): 548-550.
    4. James,L.F. and Hartley,W.J. (1977). Effect s of milk from animals feed locoweed on kittens, calves and lambs. Am J Vet Res. 38:1263-1265.
    5. Li, J.K. (2003) .Research and prospect of locoweed of China. Agric. Sci. China.36(9):1091-1099.
    6. Liu,Z.Y., Zhao,B.Y., Sun,L.S,,Wan,X.P. and Huo, X.H. (2008). Research advances in swainsonine resources and its detection methods. Chinese Vet. Sci. 38(02):175-180.
    7. Lu,H.(WangÿJ.J., Sun,L.S.)Zhao,B.Y., Wang and Z.X., Ma,R. (2009). Progress on Antineoplastic Activity of Swainsonine. Prog. Vet. Medicine. 30(9) :87-90.
    8. Song,Y.M., Wang,J.H., Wang,A.H., Wang,Y. and Yu,Y.T. (2010).The determination of swainsonine in rabbit serum by gas chromatography. Chinese Journal of Veterinary Science. 30(3): 402-405.
    9. Tong, D.W. (2000).Studies on synthesis of swainsonine of swainsonine - BSA and its immunogenicity.Northwest A & F University, PhD thesis of Materials physics and chemistry professional.
    10. Wang,K., MO,C.H., Zhao,B.Y. and Cao,G.R.. (1999).”Jifang No. E” prevention sheep Poisoning of Gansu oxytropis.Anim. Husb. Vet. Medicine. 3:31-32.
    11. Wang,S., Chen,G.Y., Hu,J.J. and Ma,C.H. (2011). Determination of Swainsonine Content in Oxytrpisglabra DC in South Xinjiang by Internal Standard Gas Chromatography. Xinjiang Agric. Sci.48(4):728-733.
    12. Wu, D., Liang, B., Feng, Z.G. and Shi,Y.P. (2003). Research progress of swainsonine. Chinese Traditional Vet. Medicine.2:42-45.
    13. Yang, M. Q.,Cao (G.R.,Li), S.J. (1998). Extraction of swainsonine of the beans Rhizoctonia. J. Northwestern Agric. Univ. 26(4): 87-90.
    14. Zhao B.Y., Fan, Y.Y. Fan, Z.F. Dong, Q. and Cao, G.R. (2006). Hazards of locoweed in the west grassland of China and the control of animals loco-toxicosis. Grass-Feeding Livestock.130:12–15.
    15. Zhao, B.Y.. (2007).The Northwest Agricultural developed grassland poisonous locoweed antidote.1:66.
    16. Zhao,J.,Tong, D.W., Shui, Y.Y., Zhao, B.Y., Liu, W.M. and Li,L. (2006).Compariso-n of Swainsonine-Extracting Technologies from Oxytropiskansuensis Bunge.ActaBotanicaBoreali-OccidentaliaSinica.26(7):1459-1463.
    17. Zeng,L. and Xia,Z.N. (2002).The improvement and influence of ultrasonic and microwave irradiation on the extraction of traditional Chinese medicine. Chemical Res. Application. 14(3): 245-249.
    18. Zhao, X.H., He,X. and Wang, J.H. (2011).Determination of Swainsonine of Astragalusvariabilis Bunge by Gas Chromatography with Solvent Extraction. Acta Agriculturae Boreali-Occidentalis Sinica. 20(6):71-74
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