GENETIC DIVERSITY OF MUNGBEAN [(Vigna radiata (L.) WILCZEK)] GENOTYPES BASED ON SDS-PAGE OF ALBUMIN SEED STORAGE PROTEIN

Article Id: ARCC1277 | Page : 54 - 57
Citation :- GENETIC DIVERSITY OF MUNGBEAN [(Vigna radiata (L.) WILCZEK)] GENOTYPES BASED ON SDS-PAGE OF ALBUMIN SEED STORAGE PROTEIN.Legume Research-An International Journal.2010.(33):54 - 57
S.K.Tripathy*, Suchinnata S. Sardar, D. Lenka and S.Sahoo
Address : S.K.Sinha Molecular Laboratory, Deptt. of Plant Breeding and Genetics, College of Agriculture, OUAT,Bhubaneswar-751 003, India

Abstract

41 test genotypes including 34 improved varieties, five local land races, one wild
accession of mungbean; and one local variety of urdbean were analysed for albumin seed
storage proteins by SDS-PAGE. The electrophoregrams revealed eleven polypeptide bands
for albumin which has shown a great array of polymorphism in presence/ absence of bands
as well as the intensity/ thickness of bands. The test genotypes were distributed over six
clusters. The cluster analysis based on, albumin polypeptide banding patterns revealed
different separate clusters for genotypes e.g., Pant M-5, RCM 15, OGG 57, OUM 7, COGG-
912 and ML 613 indicating their genetic divergence from rest of the genotypes. These
genotypes could serve as valuable breeding material for further crop improvement.

Keywords

Genetic diversity Albumin seed storage proteins SDS-PAGE Mungbean[Vigna radiata (L.)].

References

  1. Ashghar, Rehana et al (2003). Pak J Biol Sci, 6(24): 1991-1995.
  2. Ghafoor, A. et al (2002). Euphytica, 123(3): 367-378.
  3. Ghallab, K.H. et al (2007). Egyptian J. Desert Res., 57(2) : 1-11.
  4. Jacord, P.(1908). Bulletin Soc Vaud Sci National ,44: 223-270.
  5. Laemmli, U.K.(1970). Nature, 227: 680-685.
  6. Naik, B.S.(1998). Ph.D.Thesis, Utkal University, Vani Vihar, Bhubaneswar. p 52-73.
  7. Roy M.(2003). Ph.D. Thesis, Deptt. of Genetics, BCKV, kalyani West Bengal, p 82-87.
  8. Sneath, P.H.A. and Sokal, R.R.(1973). Numerical Taxonomy. W.H.Freeman & Co., San Francisco, p 573.
  9. Tomooka, N.; et al (1992). Theor. Apple. Genet. 83: 289-293

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