The haematological and biochemical parameters of both groups are presented in Table 1 and Table 2, respectively. Dogs from both groups showed a borderline reduction in the Hb, PCV and TEC levels and followed a non-significant yet apparently increasing trend in their values post-treatment. The borderline levels of Hb, PCV and TEC on day 0 observed in this study may be due to loss of blood during scratching and loss of protein through the skin (
Chakraborty and Pradhan, 2015) or poor nutrition as dogs are distracted from eating because of scratching. The apparent increase in the Hb, PCV and TEC in this study can be due to an improvement in skin lesions as a result of a reduction in mite count due to the miticidal action of doramectin. The amelioration in the Hb, PCV and TEC after treatment on subsequent days observed in this study is in accordance with the findings of
Chakraborty and Pradhan, (2015);
Chander et al. (2020) and
Patowary et al. (2022); and can be attributed to improved skin lesions, appetite and general health.
All the demodectic dogs showed leucocytosis accompanied by neutrophilia, eosinophilia and lymphopenia. Post-treatment the leukogram improves back to the normal range. The increase in TLC and absolute neutrophil count was an inflammatory response to the over-proliferating mites and their levels recuperated to normal as the mite burden reduces
(Chander et al., 2020). The eosinophilia was due to the irritation of the skin tissues because of
Demodex spp. mite infestation which stimulated the mast cells to release more histamine and since the histamine is chemotactic for eosinophils, eosinophilia developed (
Chakraborty and Pradhan, 2015). Also, lymphopenia observed in demodicosis can be ascribed to a phenomenon known as T-cell suppression resulting from certain blastogenesis-suppressing factors
i.e., various cytokines altered in demodectic dogs
(Ferrer et al., 2014 and
Chakraborty and Pradhan, 2015). A highly significant reduction in TLC, absolute neutrophil count and absolute eosinophil count on day 0, day 14, day 28 and day 42 was observed in both groups and can be due to an improvement in skin lesions and general health of the dogs as a result of a reduction in mite count due to doramectin. Also, hydroxyzine, an antihistamine, given as supportive therapy in this study may have played a role in reducing the eosinophilia as it competes with histamine for the site on H1-receptors on effector cells and antagonizes the effect of histamines (
Plumb, 2011). The highly significant increase in the absolute lymphocyte count observed in this study can be due to an improvement in skin lesions and the general health of the dogs as a result of a reduction in mite count due to doramectin. Also, levamisole played a role in restoring lymphocyte levels as it restores cell-mediated immune function in peripheral T-lymphocytes and stimulates phagocytosis by monocytes (
Plumb, 2011). On day 14, day 28 and day 42 the TLC of group I differed highly significantly from group II such that later had a comparably lower TLC count. Similarly, the absolute neutrophil count of group I differ highly significantly from group II on day 14 and day 28 and on day 42 it differs significantly such that the absolute neutrophil count of group II was comparably lower than group I. With respect to the absolute lymphocyte count, group II had significantly higher levels on day 14 than group I. However, a non-significant difference was observed between the absolute lymphocyte count of group I and group II on day 28 and day 42.
Absolute monocyte count, ALT, AST and total protein did not show a significant variation and were in the normal range. Demodectic dogs showed hypoalbuminemia, hyperglobulinemia and a reduced A:G ratio before treatment and a highly significant improvement was seen on day 42.
Chander et al. (2020) ascribed the decreased serum albumin levels to the excessive breakdown of proteins due to skin trauma and mites’ proliferation. The improvement in Albumin, Globulin and A:G ratio after treatment can be attributed to the resolution of infection.
The pre and post-treatment values of ANAE-positive cells of both groups is presented in Table 3. As implied by
Bayraktaroğlu et al. (2015) ANAE-positive cells represent T lymphocytes. The lower levels of ANAE-positive cells are thus indicative of reduced T lymphocyte levels, a feature also documented by
Ferrer et al. (2014) in demodectic dogs. On day 0, ANAE-positive cells
i.e., T lymphocyte levels were highly significantly lower in group I and group II than in the healthy control group. Post-treatment on day 42, dogs from group II showed comparable ANAE-positive cell values
i.e., T lymphocyte levels to that of the healthy control group and dogs from group I, though exhibited a rise in ANAE-positive cells
i.e., T lymphocytes in comparison to their day 0 values, showed ANAE-positive cells
i.e., T lymphocyte levels lower than that of dogs from the group II and healthy control group. A highly significant rise in ANAE-positive cells
i.e., T lymphocytes in group I can be because of the reduction in mite burden due to the miticidal action of doramectin
(Ferrer et al., 2014). A highly significant rise in ANAE-positive cells
i.e., T lymphocytes in group II can be because of the combined effect of the reduction in mite burden due to the miticidal action of doramectin and the immunostimulatory action of levamisole (
Plumb, 2011 and
Ferrer et al., 2014).
Dogs from group I and group II showed a highly significant reduction in mean mite count from 8.67±1.26 and 8.67±0.42 on day 0 to 0.17±0.17 and 0.17±0.17 on day 42, respectively. This can be attributed to the potent miticidal action of doramectin as documented by
Hutt et al. (2015);
Cordero et al. (2018) and
Parwari et al. (2022). The average number of days required to achieve 1
st negative skin scraping was 44.33±2.33 days and 35.00±4.78 days for group I and group II respectively. This was comparable with the findings of
Parwari et al. (2022) and
Hutt et al. (2015) who reported the mean rate of recovery to be 5.75±0.37 weeks and 7.1 weeks, respectively. However, statistical analysis indicated a non-significant difference in the rate of recovery between group I and group II. Out of 6 dogs from Group I and 6 dogs from Group II, 5 dogs from each group achieved negative skin scraping on/by day 42. In other words, both groups showed 88.33 % recovery on/by day 42. These findings resonate with the findings of
Hutt et al. (2015) and
Cordero et al. (2018).
Hutt et al. (2015) who recorded a 94.8% remission rate with weekly subcutaneous injections of doramectin. Similarly,
Cordero et al. (2018) recorded an 81% success rate for weekly subcutaneous injections of doramectin and a 9% success rate for weekly oral administration of doramectin. The per cent reduction in mite count, on day 14, day 28 and day 42 with respect to the baseline mite count of day 0 was 44.29%, 80.74% and 98.04% in group I and 50.06%, 92.27% and 98.04% in group II, respectively.
Dogs treated with doramectin alone and in combination with levamisole group both showed significant improvement in comparison to their day 0. However, a non-significant difference was observed between the rates of recovery for both groups and also the per cent recovery and per cent reduction in mite count were comparable to each other. The ANAE-positive cell
i.e., T-lymphocyte levels improved highly significantly in both groups in comparison to their initial levels and post-treatment ANAE-positive cells
i.e., T-lymphocytes levels were comparably higher in group II than group I. Post-treatment the haematological and biochemical parameters showed improvement in group I and group II both, however, did not differ significantly from each other. From this study, we can say that the use of levamisole does improve T-lymphocyte levels in demodectic dogs but doesn’t contribute to the parasitological recovery of the dog.