Detection of cow milk paneer in mixed/buffalo milk paneer through conventional species specific Polymerase Chain Reaction

DOI: 10.18805/ijar.9491    | Article Id: B-3169 | Page : 962-966
Citation :- Detection of cow milk paneer in mixed/buffalo milk paneer through conventional species specific Polymerase Chain Reaction .Indian Journal Of Animal Research.2017.(51):962-966

Tanmay Hazra, Vivek Sharma, Rekha Sharma, S.De, Sumit Arora and Darshan Lal
Address :

ICAR-National Dairy Research Institute, Karnal-132 001, India.

Submitted Date : 16-01-2016
Accepted Date : 3-02-2016


Due to higher market demand of buffalo milk paneer, lower price cow milk is often adulterated with higher cost buffalo milk for preparation of paneer. Till date no rapid technique is available in market to ensure that paneer is made from buffalo milk. Currently a PCR based method has been developed to authenticate the buffalo milk paneer. DNA was isolated from paneer by DNeasy Mericon food kit. A set of bovine specific primers (P1) targeting D-loop (displacement loop) of                            mt- DNA was selected and standardized to amplify cow DNA resulted 126bp amplicon. Using this PCR based approach even upto 1% level of cow milk adulteration in buffalo milk paneer could be detected. 


Food kit Mitochondrial gene Paneer PCR Species Specific primer Universal primer.


  1. Bottero, M.T. and Dalmasso, A. (2010). Animal species identification in food products: Evolution of biomolecular methods. The Vet. J. , 190 : 34–38.
  2. Cheng ,Y., Chen, S. and Weng, C. (2006). Investigation of Goats’ Milk Adulteration with Cows’ Milk by PCR. Asian-Aust. J. Anim Sci., 19 : 1503 – 1507.
  3. De, S., Brahma, B., Polley, S., Mukherjee, A., Banerjee,D. and Gohaina, M. (2011). Simplex and duplex PCR assays for species specific identification of cattle and buffalo milk and cheese. Food Cont., 22: 690–696.
  4. De , S. (2005). Outlines of Dairy Technology. Oxford University Press, New Delhi.
  5. De , S. , Singh, R. K. , Gupta, P. K. , Palia, S. and Butchaiah, G. (2000) Genotyping of dairy animals using DNA from milk somatic cells. Ind Jour of Ani Sci. 70 : 944-946.
  6. Khan, S. U. and Pal. M. A. (2011). Paneer production: A review. J.Food.Sci.Tech ., 48 : 645–660.
  7. Kocher,T.D., Thomas, W.K., Meyer, A., Edwards, S.V., Pabo, S., Villablanca, F.X. and Wilson, A.C. (1989). Dynamics of mitochondrial DNA evolution in mammals: amplification & sequencing with conserved primers. Proc. Natl. Acad. Sci., 86: 6196- 6200.
  8. Lockley, A. K. and Bardsley, R. G. (2000). DNA-based methods for food authentication . Trends in Food Sci & Tech., 11: 67-77.
  9. Maudet, C. and Taberlet, P. (2001). Detection of cows’ milk in goats’ cheeses inferred from mitochondrial DNA polymorphism. J. of Dairy Res ., 68 : 229-235.
  10. Masud ,T., Athar, I. H. and Shah, M. A. (1992) Comparative study of paneer making from buffalo and cow milk. Asian Australian J. Of Ani. Sci. , 5: 563-565
  11. Reeta., Kumar, A. and Kumbhar, B.K. (2012). Study of Sensory and Textural Properties of Paneer Using Edible Coating. Open Acc. Scien Reports., 1:15-18.
  12. Sambrook,J.and Russell, D.W. (1989). Prepparation and analysis of eukaryotic DNA. In molecular cloning:A laboratory manual.3rd edition.Cold spring Harbor Press , New Yark:6.1-6.62.
  13. Singh, S. and Kanawjia,S.K. (1988). Development of manufacturing technique for paneer from cow milk. Indian J. of Dairy Sci., 41: 322–325.
  14. Wong, C. M. V. L., Lim, A. C. and Chua, H. K. (2010). Detection of meat contaminants in processed meats using polymerase chain reaction-restriction fragment length polymorphismanalysis. Borneo. Sci., 2 :15-18

Global Footprints