Detection of cow milk paneer in mixed/buffalo milk paneer through conventional species specific Polymerase Chain Reaction

DOI: 10.18805/ijar.9491    | Article Id: B-3169 | Page : 962-966
Citation :- Detection of cow milk paneer in mixed/buffalo milk paneer through conventional species specific Polymerase Chain Reaction .Indian Journal Of Animal Research.2017.(51):962-966

Tanmay Hazra, Vivek Sharma, Rekha Sharma, S.De, Sumit Arora and Darshan Lal

tanmayhazra08@gmail.com
Address :

ICAR-National Dairy Research Institute, Karnal-132 001, India.

Submitted Date : 16-01-2016
Accepted Date : 3-02-2016

Abstract

Due to higher market demand of buffalo milk paneer, lower price cow milk is often adulterated with higher cost buffalo milk for preparation of paneer. Till date no rapid technique is available in market to ensure that paneer is made from buffalo milk. Currently a PCR based method has been developed to authenticate the buffalo milk paneer. DNA was isolated from paneer by DNeasy Mericon food kit. A set of bovine specific primers (P1) targeting D-loop (displacement loop) of                            mt- DNA was selected and standardized to amplify cow DNA resulted 126bp amplicon. Using this PCR based approach even upto 1% level of cow milk adulteration in buffalo milk paneer could be detected. 

Keywords

Food kit Mitochondrial gene Paneer PCR Species Specific primer Universal primer.

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