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Current IssueEditorial BoardOnline First ArticlesArchive

volume 44 issue 4 (december 2010) : 280 - 284

BACTERIOLOGY OF SUB CLINICAL MASTITIS AND ANTIBIOGRAM OF ISOLATES RECOVERED FROM CROSS BRED COWS

A
A. Elango
K
K.A. Doraisamy*
G
G. Rajarajan
G
G. Kumaresan
1Dept. of Dairy Science, Veterinary College & Research Institute, Namakkal-637 002, India.

  • Submitted|

  • First Online |

  • doi

Cite article:- Elango A., Doraisamy* K.A., Rajarajan G., Kumaresan G. (2025). BACTERIOLOGY OF SUB CLINICAL MASTITIS AND ANTIBIOGRAM OF ISOLATES RECOVERED FROM CROSS BRED COWS. Indian Journal of Animal Research. 44(4): 280 - 284. doi: .

ABSTRACT

A total of 178 milk samples from apparently healthy lactating crossbred cows (98
Jersey cross and 80 Holstein Friesian cross) were screened for sub-clinical mastitis. The
physical examination of sub clinical mastitic milk revealed no changes in colour, consistency
and odour. The chemical examination revealed increased pH, somatic cell count and chloride
content and positive scores on modified california mastitis test. Out of 98 Jersey crosses
and 80 Holstein Friesian crosses which were subjected to examination 30 Jersey crosses(30.61%)
and Holstein Friesian crosses 27(33.75%) were found to be affected with subclinical mastitis
with a cumulative incidence of 32.02% in cross bred cows. The isolated pathogens from
sub-clinical cases and their relative frequencies were: Staphylococcus sp. (38.59%),
Streptococcus sp. (35.08%), Escherichia coli (12.28%), Corynebacterium pyogens (8.77%)
and Bacillus sp. (5.26%). The in vitro antibiogram revealed Ceftriaxone(92.66%),
Enrofloxacin(89.83%), Kanamycin (88.13%), Ciprofloxacin (82.3%) were most effective,
Gentamicin (63.16%) and Chloramphenicol (55.93%) were moderately effective and
Tetracycline (32.20%), Ampicillin(30.50%) and Erythromycin (28.81%) were the least effective
drugs.

REFERENCES

  1. Ahlner, S. and Axelsson, A. (2002) Analysis of bacterial growth and the prevalence of sub-clinical mastitis from bulk
  2. tank samples and individual samples from dairy herds in a region in Uruguay.Minor-Field-Studies-International-
  3. Office,-Swedish-University-of-AgriculturalSci., 214: 16
  4. Bauer, A.W. et al. (1966). Am. J. Clin. Pathol., 45: 493-496.
  5. BIS: SP: 18 (Part XI) (1981). Handbook of Food Analysis, Bureax of Indian Shandards, New Delhi.
  6. Boynukara, B. et al.(2008). Intl. J. of Food Microbiol.,125(2):209-211
  7. Buragohain, J. and Dutta, G.N. (1998). Indian J. Comp. Immunol. Infec. Dis., 19: 53-59.
  8. Charkrabati, A.(2004) A textbook of Preventive Veterinary Medicine, 3th Ed. Kalyani Publication, New Delhi. pp.513-
  9. 516.
  10. Dobbins, C.N., (1977) J. Amer. Vet. Med. Assoc., 170 : 1129 - 1132.
  11. Doxey, D.L.,(1983)Clinical Pathology and Diagnostic Procedures 2nd Ed. Bailiere Tindall, London. pp.129, 293
  12. Jasper, D. E. et al. (1982). Bovine mastitis research needs funding and sources of support. In: Proceeding of 21st Annual
  13. Meeting, National Mastitis Council Inc., Louisville, Kentucky, USA.pp: 182-193.
  14. Joshi, S and Gokhale, S. (2004). Status of mastitis as an emerging disease in improved and periurban dairy farms in
  15. India. 23rd World Buiatrics Congress Quebec city, Canada.
  16. Kitchen, B.J.(1981).J. Dairy Sci., 48:167-188.
  17. Martin, R.P. et al. (2006) Hygiene Alimentar., 20: 139.
  18. Muhammad, G. et al. (1995). Pakistan J.Food Sci., 5: 91–3
  19. Narayana, T. and Iya, K.K. (1954). Indian J. Dairy Sci., 6: 169-179
  20. Paul, W.M. et al.(2000). Indian. J. Anim. Health., 39 (2): 79- 81.
  21. Philpot, W.N and Nickerson, S (1991) Mastitis attack. Surge International – Babson Bros. Co. Naperville, Illinois,
  22. U.S.A.
  23. Quinn, P.J. et al.(2002) Veterinary microbiology and microbial diseases MPG Books Ltd, Cornwall. pp.23-28
  24. Radostitis, D.M. et al.(2000) Veterinary Medicine. A Textbook of Diseases of Cattle, Sheep, Pigs, Goats and Horses,
  25. 9th Ed. London. pp.612
  26. Reneau, J. K.,(1986).J. Dairy Sci., 69: 1708-1720.
  27. Saravanan, P., (1997). A comparative study on the efficacy of diagnostic tests in the diagnosis of bovine subclinical
  28. mastitis. M.V.Sc., Thesis submitted to Tamil Nadu Veterinary and Animal Science University, Chennai.
  29. Sharma,N. et al.(2007) Intl. J. Dairy Sci., 2 (2): 145-151.
  30. Sharma, V.K. and Rajani, H.B. (1969)Indian Vet. J., 46: 749-752.
  31. Schalm, O.W. et al.(1971). Bovine Mastitis. Lea and Febiger, Philadelphia. pp. 102-103.
  32. 284 INDIAN JOURNAL OF ANIMAL RESEARCH
  33. Sheldrake, R.F. and Hoare, R.J.T. (1981). Newzeland.Vet. J., 29 : 211-213.
  34. Shrirame,K.R., Bhardwaj, S.S., Kalorey,D.R. and Harne,S.D(2002).Study of pathogenicity markers of Staphyloococci
  35. from subclinical mastitis in Proceedings of Round table Conference,Feb.4-5,Nagpur,India.pp.18-22.
  36. Tiwari, A and Sisodia, R.S. (2000).Intas Polivet., 1(1): 96-100.
  37. Vijaykumar, K, (2003). Tests for detection of mastitis. In: Proceeding Indian Veterinary Congress X Annual 4 th Round
  38. Table on mastitis 14-15 April, College of Veterinary Science and Animal Husbandry, C.S.K.H.P.K.V, Palampur
  39. (H.P.). pp.137-145.
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    volume 44 issue 4 (december 2010) : 280 - 284

    BACTERIOLOGY OF SUB CLINICAL MASTITIS AND ANTIBIOGRAM OF ISOLATES RECOVERED FROM CROSS BRED COWS

    A
    A. Elango
    K
    K.A. Doraisamy*
    G
    G. Rajarajan
    G
    G. Kumaresan
    1Dept. of Dairy Science, Veterinary College & Research Institute, Namakkal-637 002, India.

    • Submitted|

    • First Online |

    • doi

    Cite article:- Elango A., Doraisamy* K.A., Rajarajan G., Kumaresan G. (2025). BACTERIOLOGY OF SUB CLINICAL MASTITIS AND ANTIBIOGRAM OF ISOLATES RECOVERED FROM CROSS BRED COWS. Indian Journal of Animal Research. 44(4): 280 - 284. doi: .

    ABSTRACT

    A total of 178 milk samples from apparently healthy lactating crossbred cows (98
    Jersey cross and 80 Holstein Friesian cross) were screened for sub-clinical mastitis. The
    physical examination of sub clinical mastitic milk revealed no changes in colour, consistency
    and odour. The chemical examination revealed increased pH, somatic cell count and chloride
    content and positive scores on modified california mastitis test. Out of 98 Jersey crosses
    and 80 Holstein Friesian crosses which were subjected to examination 30 Jersey crosses(30.61%)
    and Holstein Friesian crosses 27(33.75%) were found to be affected with subclinical mastitis
    with a cumulative incidence of 32.02% in cross bred cows. The isolated pathogens from
    sub-clinical cases and their relative frequencies were: Staphylococcus sp. (38.59%),
    Streptococcus sp. (35.08%), Escherichia coli (12.28%), Corynebacterium pyogens (8.77%)
    and Bacillus sp. (5.26%). The in vitro antibiogram revealed Ceftriaxone(92.66%),
    Enrofloxacin(89.83%), Kanamycin (88.13%), Ciprofloxacin (82.3%) were most effective,
    Gentamicin (63.16%) and Chloramphenicol (55.93%) were moderately effective and
    Tetracycline (32.20%), Ampicillin(30.50%) and Erythromycin (28.81%) were the least effective
    drugs.

    REFERENCES

    1. Ahlner, S. and Axelsson, A. (2002) Analysis of bacterial growth and the prevalence of sub-clinical mastitis from bulk
    2. tank samples and individual samples from dairy herds in a region in Uruguay.Minor-Field-Studies-International-
    3. Office,-Swedish-University-of-AgriculturalSci., 214: 16
    4. Bauer, A.W. et al. (1966). Am. J. Clin. Pathol., 45: 493-496.
    5. BIS: SP: 18 (Part XI) (1981). Handbook of Food Analysis, Bureax of Indian Shandards, New Delhi.
    6. Boynukara, B. et al.(2008). Intl. J. of Food Microbiol.,125(2):209-211
    7. Buragohain, J. and Dutta, G.N. (1998). Indian J. Comp. Immunol. Infec. Dis., 19: 53-59.
    8. Charkrabati, A.(2004) A textbook of Preventive Veterinary Medicine, 3th Ed. Kalyani Publication, New Delhi. pp.513-
    9. 516.
    10. Dobbins, C.N., (1977) J. Amer. Vet. Med. Assoc., 170 : 1129 - 1132.
    11. Doxey, D.L.,(1983)Clinical Pathology and Diagnostic Procedures 2nd Ed. Bailiere Tindall, London. pp.129, 293
    12. Jasper, D. E. et al. (1982). Bovine mastitis research needs funding and sources of support. In: Proceeding of 21st Annual
    13. Meeting, National Mastitis Council Inc., Louisville, Kentucky, USA.pp: 182-193.
    14. Joshi, S and Gokhale, S. (2004). Status of mastitis as an emerging disease in improved and periurban dairy farms in
    15. India. 23rd World Buiatrics Congress Quebec city, Canada.
    16. Kitchen, B.J.(1981).J. Dairy Sci., 48:167-188.
    17. Martin, R.P. et al. (2006) Hygiene Alimentar., 20: 139.
    18. Muhammad, G. et al. (1995). Pakistan J.Food Sci., 5: 91–3
    19. Narayana, T. and Iya, K.K. (1954). Indian J. Dairy Sci., 6: 169-179
    20. Paul, W.M. et al.(2000). Indian. J. Anim. Health., 39 (2): 79- 81.
    21. Philpot, W.N and Nickerson, S (1991) Mastitis attack. Surge International – Babson Bros. Co. Naperville, Illinois,
    22. U.S.A.
    23. Quinn, P.J. et al.(2002) Veterinary microbiology and microbial diseases MPG Books Ltd, Cornwall. pp.23-28
    24. Radostitis, D.M. et al.(2000) Veterinary Medicine. A Textbook of Diseases of Cattle, Sheep, Pigs, Goats and Horses,
    25. 9th Ed. London. pp.612
    26. Reneau, J. K.,(1986).J. Dairy Sci., 69: 1708-1720.
    27. Saravanan, P., (1997). A comparative study on the efficacy of diagnostic tests in the diagnosis of bovine subclinical
    28. mastitis. M.V.Sc., Thesis submitted to Tamil Nadu Veterinary and Animal Science University, Chennai.
    29. Sharma,N. et al.(2007) Intl. J. Dairy Sci., 2 (2): 145-151.
    30. Sharma, V.K. and Rajani, H.B. (1969)Indian Vet. J., 46: 749-752.
    31. Schalm, O.W. et al.(1971). Bovine Mastitis. Lea and Febiger, Philadelphia. pp. 102-103.
    32. 284 INDIAN JOURNAL OF ANIMAL RESEARCH
    33. Sheldrake, R.F. and Hoare, R.J.T. (1981). Newzeland.Vet. J., 29 : 211-213.
    34. Shrirame,K.R., Bhardwaj, S.S., Kalorey,D.R. and Harne,S.D(2002).Study of pathogenicity markers of Staphyloococci
    35. from subclinical mastitis in Proceedings of Round table Conference,Feb.4-5,Nagpur,India.pp.18-22.
    36. Tiwari, A and Sisodia, R.S. (2000).Intas Polivet., 1(1): 96-100.
    37. Vijaykumar, K, (2003). Tests for detection of mastitis. In: Proceeding Indian Veterinary Congress X Annual 4 th Round
    38. Table on mastitis 14-15 April, College of Veterinary Science and Animal Husbandry, C.S.K.H.P.K.V, Palampur
    39. (H.P.). pp.137-145.
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