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Current IssueEditorial BoardOnline First ArticlesArchive

volume 40 issue 4 (december 2006) : 246 - 254

GEL ELECTROPHORESIS OF EXTRACELLULAR SOLUBLE PROTEINS OF WHEAT LEAF BLOTCH FUNGUS BIOLARIS SOROKINIANA

S
S.K. Adhikary*
I
I.H. Mian
1Bangabondhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

  • Submitted|

  • First Online |

  • doi

Cite article:- Adhikary* S.K., Mian I.H. (2025). GEL ELECTROPHORESIS OF EXTRACELLULAR SOLUBLE PROTEINS OF WHEAT LEAF BLOTCH FUNGUS BIOLARIS SOROKINIANA. Indian Journal of Agricultural Research. 40(4): 246 - 254. doi: .

ABSTRACT

Gel electrophoresis of extracellular soluble proteins of Bipolaris sorokinina was done to determine the variation in protein patterns. Twenty six distinct protein patterns with a total of 28 bands were detected. Six protein bands with Ef values 0.74, 0.70, 0.65, 0.45, 0.40 and 0.31 corresponding to the molecular weight of about 32, 34, 40, 71, 79 and 100 kDa respectively, may be considered as the charactrerstic bands of B. sorokiniana among which proteins of electrophoretic values 0.74 and 0.31 were found equivalent to carbonic anhydrase and bovine seam albumin. Phenotypes of higher frequencies were found to be the representatives of different locations indicating random distribution of B. sorokiniana. Genetic similarity indices based UPGMA phenogram delineated electrohoretic protein patterns into apparently eight clusters

KEYWORDS

    REFERENCES

    1. Bakonyi, J. et al. (1998). In: Helnninthosporiuni Blights of wheat: Spot blotch and Tan spot. (Duveiller, E. et al.eds.), CIMMYT, Mexico, D.F., 80-87 pp.
    2. Bidari, V.B. and Govindu, H.C. (1975). Mysore J. Agric. Sci., 9: 87-94.
    3. Clare, B.G. and Zentmeyer, G.A. (1966). Phytopathology, 56: 1334-1335.
    4. Clare, B.G. (1963). Nature, 200: 803-804.
    5. Digby, S. and Weidemann, G.J. (1991). Phytopathology, 81: 1191 (Abstr.).
    6. Hall, R. et al. (1969). Phytopatholgy, 59: 770-774.
    7. Hetzler, J. et al. (1991). In: Wheat for the Nontraditional, Warm Areas. (Saunders, D.A. ed.), CIMMYT, Mexico,
    8. D.F., 146-164 pp.
    9. Laemmli, U.K. (1970). Nature, 227: 680-685.
    10. Macko, V. et al. (1967). Phytopath. Z., 58: 121-127.
    11. Matsuyama, N. and Kozaka, T. (1971). Ann. Phytopath. Soc. Japan, 37: 259-265.
    12. Mehta, Y.R. (1981). Oescuisa Agropecuana Brasiliera (Brasilia), 16: 331-336.
    13. Misra, A.P. et al. (1981). 3rd Inter. Symp. Plant Path., IARI, New Delhi, pp. 131-132 (Abstr.).
    14. Nei, M. and Li, W.H. (1979). Proc. Nat. Acad. Sci. USA, 76: 5269-5273.
    15. Nema, K.G. (1986). In: Problems and Progress of Wheat Pahology in South Asia. (Joshi, L.M.
    16. et al. eds.), Malhotra Publishing House, New Delhi, 162-175 pp.
    17. Pascaul, C.B. and Raymundo, A.K. (1991). Philippines J. Crop Sci., 16(1): 38 (Abstr.).
    18. Pascaul, C.B. (1992). College Laguna (Philippines) pp. 74 (Abstr.).
    19. Sivanesan. A. (1987). Mycological Papers. No. 158. Wallingford. UK: CAB International Mycological Institute.
    20. Snider, R.D and Kramer C.L. (1974). Mycologia., 66: 743-753.
    21. Whitney. P.J. et al. (1968). J. Exp. Bot., 19: 415-426.
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    Indian Journal of Agricultural Research
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      volume 40 issue 4 (december 2006) : 246 - 254

      GEL ELECTROPHORESIS OF EXTRACELLULAR SOLUBLE PROTEINS OF WHEAT LEAF BLOTCH FUNGUS BIOLARIS SOROKINIANA

      S
      S.K. Adhikary*
      I
      I.H. Mian
      1Bangabondhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

      • Submitted|

      • First Online |

      • doi

      Cite article:- Adhikary* S.K., Mian I.H. (2025). GEL ELECTROPHORESIS OF EXTRACELLULAR SOLUBLE PROTEINS OF WHEAT LEAF BLOTCH FUNGUS BIOLARIS SOROKINIANA. Indian Journal of Agricultural Research. 40(4): 246 - 254. doi: .

      ABSTRACT

      Gel electrophoresis of extracellular soluble proteins of Bipolaris sorokinina was done to determine the variation in protein patterns. Twenty six distinct protein patterns with a total of 28 bands were detected. Six protein bands with Ef values 0.74, 0.70, 0.65, 0.45, 0.40 and 0.31 corresponding to the molecular weight of about 32, 34, 40, 71, 79 and 100 kDa respectively, may be considered as the charactrerstic bands of B. sorokiniana among which proteins of electrophoretic values 0.74 and 0.31 were found equivalent to carbonic anhydrase and bovine seam albumin. Phenotypes of higher frequencies were found to be the representatives of different locations indicating random distribution of B. sorokiniana. Genetic similarity indices based UPGMA phenogram delineated electrohoretic protein patterns into apparently eight clusters

      KEYWORDS

        REFERENCES

        1. Bakonyi, J. et al. (1998). In: Helnninthosporiuni Blights of wheat: Spot blotch and Tan spot. (Duveiller, E. et al.eds.), CIMMYT, Mexico, D.F., 80-87 pp.
        2. Bidari, V.B. and Govindu, H.C. (1975). Mysore J. Agric. Sci., 9: 87-94.
        3. Clare, B.G. and Zentmeyer, G.A. (1966). Phytopathology, 56: 1334-1335.
        4. Clare, B.G. (1963). Nature, 200: 803-804.
        5. Digby, S. and Weidemann, G.J. (1991). Phytopathology, 81: 1191 (Abstr.).
        6. Hall, R. et al. (1969). Phytopatholgy, 59: 770-774.
        7. Hetzler, J. et al. (1991). In: Wheat for the Nontraditional, Warm Areas. (Saunders, D.A. ed.), CIMMYT, Mexico,
        8. D.F., 146-164 pp.
        9. Laemmli, U.K. (1970). Nature, 227: 680-685.
        10. Macko, V. et al. (1967). Phytopath. Z., 58: 121-127.
        11. Matsuyama, N. and Kozaka, T. (1971). Ann. Phytopath. Soc. Japan, 37: 259-265.
        12. Mehta, Y.R. (1981). Oescuisa Agropecuana Brasiliera (Brasilia), 16: 331-336.
        13. Misra, A.P. et al. (1981). 3rd Inter. Symp. Plant Path., IARI, New Delhi, pp. 131-132 (Abstr.).
        14. Nei, M. and Li, W.H. (1979). Proc. Nat. Acad. Sci. USA, 76: 5269-5273.
        15. Nema, K.G. (1986). In: Problems and Progress of Wheat Pahology in South Asia. (Joshi, L.M.
        16. et al. eds.), Malhotra Publishing House, New Delhi, 162-175 pp.
        17. Pascaul, C.B. and Raymundo, A.K. (1991). Philippines J. Crop Sci., 16(1): 38 (Abstr.).
        18. Pascaul, C.B. (1992). College Laguna (Philippines) pp. 74 (Abstr.).
        19. Sivanesan. A. (1987). Mycological Papers. No. 158. Wallingford. UK: CAB International Mycological Institute.
        20. Snider, R.D and Kramer C.L. (1974). Mycologia., 66: 743-753.
        21. Whitney. P.J. et al. (1968). J. Exp. Bot., 19: 415-426.
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