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Current IssueEditorial BoardOnline First ArticlesArchive

volume 40 issue 4 (december 2006) : 235 - 245

GEL ELECTROPHORESIS OF EXTRACELLULAR ENZYMES OF BIPOLARIS SOROKINIANA

S
S.K. Adhikary*
I
I.H. Mian
1Bangabondhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

  • Submitted|

  • First Online |

  • doi

Cite article:- Adhikary* S.K., Mian I.H. (2025). GEL ELECTROPHORESIS OF EXTRACELLULAR ENZYMES OF BIPOLARIS SOROKINIANA. Indian Journal of Agricultural Research. 40(4): 235 - 245. doi: .

ABSTRACT

Gel electrophoresis of extracellular enzymes of Bipolaris sorokinina was conducted to determine the isozyme variation. Of the four enzymes evaluated, three enzymes, viz, esterase, catalase and acid phosphatase were found active and resolved well. Remarkable variations were observed in all three enzyme systems. Electrophoretic phenotypes for esterase, catalase and acid phosphatase were 8, 5 and 5, respectively. The band at Rf value 0.55 was found as the unique esterase band. No unique catalase and acid phosphatase bands were detected. A total of 62 overall enzyme phenotypes were identified. Phenotypes of higher frequencies were found to be the representatives of closer to distant different locations indicating random distribution of B. sorokiniana. Cluster analysis of these phenotypes based on Euclidean distance delineated three major groups and two subgroups under each group.

KEYWORDS

    REFERENCES

    1. Anderberg, M.R. (1973). Cluster Analysis for Application. Academic Press, New York.
    2. Bhowmik, A. (1995). Gel Electrophoresis Techniques IPSA-JICA Project Pub. 25, Instt. Post graduate stud. in Agric. Bonde, M.R. et al. (1993). Plant Dis., 77(10): 961-968.
    3. Bonde, M.R. et al. (1988). Phytopathology, 78:1491-1494.
    4. Bosland, P.W. and Williams, P.H. (1987). Can. J. Bot., 65: 2067-2073.
    5. Guikema, J.A. and Sherman L.A. (1980). In: Isozymes in plant Genetics and Breeding, part A. Tanksley, S.D and Ortorn, T.J. eds. 1983, Elsevier Science Publishers B.V. Amsterdam.
    6. Harris, H. and Hopkinson, D.A. (1976). Handbook of Enzyme Electrophoresis in Human Genetics (With
    7. supplements). Oxford American Elsevier Publishing Co. New York.
    8. Heutell, R.N. et al. (1983). J. Nematol., 15: 338-344.
    9. Kaufmann, P.J. and Weidemann, G.J. (1996). Plant Dis., 80: 1289-1293.
    10. Laemmli, U.K. (1970). Nature, 227: 680-685.
    11. Leung, H. and Williams, P.H. (1986). Phytopathologly, 76: 778-783.
    12. Levy, M. et al. (1991). Plant cell, 3: 95-102.
    13. Linde, D. et al. (1997). Phytopathologly, 87: 822-827.
    14. Lui, Z. et al. (1990). Can. J. Plant Pathol., 12: 376-382.
    15. Marshall, D.R. and Brown, A.H.D. (1975). J. Mol. Ecol., 6: 149-163.
    16. Matsuyama, N. et al. (1977). Ann. Phytopath Soc. Japan, 43: 419-425.
    17. Matsuyama, N. and Kozaka, T. (1971). Ann. Phytopath Soc. Japan, 37: 259-265.
    18. Matsuyama, N. and Wakimoto, S. (1977). Ann. Phytopath Soc. Japan, 43: 462-470.
    19. Matsuyama, N. et al. (1978). Ann. Phytopath Soc. Japan, 44: 652-658.
    20. Micales, J.A. et al. (1986). Mycotaxon, 27: 405-449
    21. Micales, J.A. et al. (1988). Phytopathology, 78: 1396-1402.
    22. Nei, M. and Li, W.H. (1979). Proc. Nat. Acad. Sci. USA, 76: 5269-5273.
    23. Norusis, K.J. (1993). SPSS for Windows-Professional Statistics, Release 6.0 SPSS Inc. 444N. Michigan avenue, Chicago. Illinoise, 6061.
    24. Powell, J.R. (1975). In Evolutionary Biology. Vol. 8 (Dobzhanzky, T. et al., eds). Plenum Press, New York, pp. 79-119.
    25. Powers, H.R. Jr. et al. (1989). Plant Dis., 73: 691-694.
    26. Powers, H.R. Jr. et al. (1991). Plant Dis., 75: 1225-1227.
    27. Raemaekers, R.H. (1985). In: Wheats for More Tropical Environments. Mexico, D.F.: CIMMYT. A proceedings of
    28. a symposium, 201-203 pp.
    29. Romesburg, H.C. (1984). Cluster Analysis Researchers. Belmont, Calif: Lifetime Learning Publication.
    30. Simcox, K.D. et al. (1992). Phytopathology, 82: 621-624.
    31. Simcox, K.D. et al. (1993). Can. J. Plant Pathol., 15: 91-96.
    32. Spielman, L.J. et al. (1990). Exp. Mycol., 14: 57-69.
    33. Tanksley, S.D. and Orton, T.J. (1984). Isozymes in Plant Genetics and Breeding. North-Holland Publ. Co. Amsterdam.
    34. Tooley, P.W. et al. (1985). J. Heredity, 76: 431-435.
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    Indian Journal of Agricultural Research
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      volume 40 issue 4 (december 2006) : 235 - 245

      GEL ELECTROPHORESIS OF EXTRACELLULAR ENZYMES OF BIPOLARIS SOROKINIANA

      S
      S.K. Adhikary*
      I
      I.H. Mian
      1Bangabondhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

      • Submitted|

      • First Online |

      • doi

      Cite article:- Adhikary* S.K., Mian I.H. (2025). GEL ELECTROPHORESIS OF EXTRACELLULAR ENZYMES OF BIPOLARIS SOROKINIANA. Indian Journal of Agricultural Research. 40(4): 235 - 245. doi: .

      ABSTRACT

      Gel electrophoresis of extracellular enzymes of Bipolaris sorokinina was conducted to determine the isozyme variation. Of the four enzymes evaluated, three enzymes, viz, esterase, catalase and acid phosphatase were found active and resolved well. Remarkable variations were observed in all three enzyme systems. Electrophoretic phenotypes for esterase, catalase and acid phosphatase were 8, 5 and 5, respectively. The band at Rf value 0.55 was found as the unique esterase band. No unique catalase and acid phosphatase bands were detected. A total of 62 overall enzyme phenotypes were identified. Phenotypes of higher frequencies were found to be the representatives of closer to distant different locations indicating random distribution of B. sorokiniana. Cluster analysis of these phenotypes based on Euclidean distance delineated three major groups and two subgroups under each group.

      KEYWORDS

        REFERENCES

        1. Anderberg, M.R. (1973). Cluster Analysis for Application. Academic Press, New York.
        2. Bhowmik, A. (1995). Gel Electrophoresis Techniques IPSA-JICA Project Pub. 25, Instt. Post graduate stud. in Agric. Bonde, M.R. et al. (1993). Plant Dis., 77(10): 961-968.
        3. Bonde, M.R. et al. (1988). Phytopathology, 78:1491-1494.
        4. Bosland, P.W. and Williams, P.H. (1987). Can. J. Bot., 65: 2067-2073.
        5. Guikema, J.A. and Sherman L.A. (1980). In: Isozymes in plant Genetics and Breeding, part A. Tanksley, S.D and Ortorn, T.J. eds. 1983, Elsevier Science Publishers B.V. Amsterdam.
        6. Harris, H. and Hopkinson, D.A. (1976). Handbook of Enzyme Electrophoresis in Human Genetics (With
        7. supplements). Oxford American Elsevier Publishing Co. New York.
        8. Heutell, R.N. et al. (1983). J. Nematol., 15: 338-344.
        9. Kaufmann, P.J. and Weidemann, G.J. (1996). Plant Dis., 80: 1289-1293.
        10. Laemmli, U.K. (1970). Nature, 227: 680-685.
        11. Leung, H. and Williams, P.H. (1986). Phytopathologly, 76: 778-783.
        12. Levy, M. et al. (1991). Plant cell, 3: 95-102.
        13. Linde, D. et al. (1997). Phytopathologly, 87: 822-827.
        14. Lui, Z. et al. (1990). Can. J. Plant Pathol., 12: 376-382.
        15. Marshall, D.R. and Brown, A.H.D. (1975). J. Mol. Ecol., 6: 149-163.
        16. Matsuyama, N. et al. (1977). Ann. Phytopath Soc. Japan, 43: 419-425.
        17. Matsuyama, N. and Kozaka, T. (1971). Ann. Phytopath Soc. Japan, 37: 259-265.
        18. Matsuyama, N. and Wakimoto, S. (1977). Ann. Phytopath Soc. Japan, 43: 462-470.
        19. Matsuyama, N. et al. (1978). Ann. Phytopath Soc. Japan, 44: 652-658.
        20. Micales, J.A. et al. (1986). Mycotaxon, 27: 405-449
        21. Micales, J.A. et al. (1988). Phytopathology, 78: 1396-1402.
        22. Nei, M. and Li, W.H. (1979). Proc. Nat. Acad. Sci. USA, 76: 5269-5273.
        23. Norusis, K.J. (1993). SPSS for Windows-Professional Statistics, Release 6.0 SPSS Inc. 444N. Michigan avenue, Chicago. Illinoise, 6061.
        24. Powell, J.R. (1975). In Evolutionary Biology. Vol. 8 (Dobzhanzky, T. et al., eds). Plenum Press, New York, pp. 79-119.
        25. Powers, H.R. Jr. et al. (1989). Plant Dis., 73: 691-694.
        26. Powers, H.R. Jr. et al. (1991). Plant Dis., 75: 1225-1227.
        27. Raemaekers, R.H. (1985). In: Wheats for More Tropical Environments. Mexico, D.F.: CIMMYT. A proceedings of
        28. a symposium, 201-203 pp.
        29. Romesburg, H.C. (1984). Cluster Analysis Researchers. Belmont, Calif: Lifetime Learning Publication.
        30. Simcox, K.D. et al. (1992). Phytopathology, 82: 621-624.
        31. Simcox, K.D. et al. (1993). Can. J. Plant Pathol., 15: 91-96.
        32. Spielman, L.J. et al. (1990). Exp. Mycol., 14: 57-69.
        33. Tanksley, S.D. and Orton, T.J. (1984). Isozymes in Plant Genetics and Breeding. North-Holland Publ. Co. Amsterdam.
        34. Tooley, P.W. et al. (1985). J. Heredity, 76: 431-435.
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