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volume 43 issue 2 (june 2009) : 129·133

IDENTIFICATION OF ACTIVE PRINCIPLE IN THE HERBAL EXTRACTS POSSESSING BACTERICIDAL ACTION AGAINST CITRUS CANKER, XANTHOMONAS AXONOPODIS P\l. CITRI.

K
K. Manonmani
M
M. Jayasekhar
C
C. Gailce Leo Justin
T
T. Thangaselvabai
1Krishi Vigyan Kendra, Horticultural Research Station (TNAU), Pechiparai-629161, India.

  • Submitted|

  • First Online |

  • doi

Cite article:- Manonmani K., Jayasekhar M., Justin Leo Gailce C., Thangaselvabai T. (2025). IDENTIFICATION OF ACTIVE PRINCIPLE IN THE HERBAL EXTRACTS POSSESSING BACTERICIDAL ACTION AGAINST CITRUS CANKER, XANTHOMONAS AXONOPODIS P\l. CITRI.. Indian Journal of Agricultural Research. 43(2): 129·133. doi: .

ABSTRACT

Bactericidal action ofsome selected herbllll aIracts viz., Acalypha indica, Achyranthes
aspera, Aloe vera, Azadirachta indica, Datura metal, Hibiscus rosasinensis, Nerium
oleander, Ocimum sanctum, O. basilicum, Phyllanthus emblica, Polyalthia longifolia, Piper
betle, Punica granatum, Solanum torvum and Solanum trilobatum were tested in vitro
against the growth of citrus canker disease causing pathogen, Xanthomonas axonopodis
pv. citri (Xac) .. The leaf extract of P. longifolia followed by A. aspera recorded the
maximum growth inhibition of 9.77mm and 8.47mm diameter respectively. The
20-40% ammonium sulphate fraction of A. aspera and 40-60% fraction of P. longifolia
exhibited inhibitory action on in vitro growth of the pathogen. The molecular
weight of the fractions of leaf extracts was found to be 30 kDa and 19 kDa for
P. long;folia and A. aspera respectively.

REFERENCES

  1. Balasaraswathy, R (1995). Ph.D. Thesis, Tamil Nadu Agri. Univ., Coimbatore, India.
  2. Bradford, M.M. (1976). Anal. Biochem., 72:248-254.
  3. Chandramohan, B. and Moses, G.J. (1989). In: Seminar on Diseases of Fruit Crops. Tirupati. 81.
  4. Harini, SA, (2000). M.Sc., (Ag.)Thesis, Tamil Nadu Agri. Uni., Coimbatore, India.
  5. Kodama, K. et al., (1991). Int. Symp. Tea Sci. (ISTS):294-298.
  6. Laemmli, U.K. (1970). Nature, 227:680-685.
  7. Majd, A. and Mehrabian, S. (2002). In: XXVI Int. Horti. Congress, Toronto, Canada, August, 11-17.
  8. Muraro, RP. (1986). Food and Resource Econ. Dep., Institute of Food and Agri. Sci., Uni. Florida. Staff Paper 289.
  9. Premananth, K. (1997). M.Sc. (Ag.) Thesis, Tamil Nadu Agri. Univ., Coimbatore, Tamil Nadu, India.
  10. Pruvost,o. etal., (2002). Phytopathology, 92:336-346.
  11. Rajeswari, E. (1991). M.Sc. (Ag.) Thesis, Tamil Nadu Agri. Univ., Coimbatore, India.
  12. Shekawat, P.S. and Prasad, R (1971). Indian Phytopath., 24:800-802.
  13. Sivasankar, A. (1994). M.Sc. (Ag.) Thesis, Tamil Nadu Agri. Univ., Coimbatore, India.
  14. Smale, B.C. and Keil, H.C. (1966). Phytochemistry, 5:1113-1120.
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    volume 43 issue 2 (june 2009) : 129·133

    IDENTIFICATION OF ACTIVE PRINCIPLE IN THE HERBAL EXTRACTS POSSESSING BACTERICIDAL ACTION AGAINST CITRUS CANKER, XANTHOMONAS AXONOPODIS P\l. CITRI.

    K
    K. Manonmani
    M
    M. Jayasekhar
    C
    C. Gailce Leo Justin
    T
    T. Thangaselvabai
    1Krishi Vigyan Kendra, Horticultural Research Station (TNAU), Pechiparai-629161, India.

    • Submitted|

    • First Online |

    • doi

    Cite article:- Manonmani K., Jayasekhar M., Justin Leo Gailce C., Thangaselvabai T. (2025). IDENTIFICATION OF ACTIVE PRINCIPLE IN THE HERBAL EXTRACTS POSSESSING BACTERICIDAL ACTION AGAINST CITRUS CANKER, XANTHOMONAS AXONOPODIS P\l. CITRI.. Indian Journal of Agricultural Research. 43(2): 129·133. doi: .

    ABSTRACT

    Bactericidal action ofsome selected herbllll aIracts viz., Acalypha indica, Achyranthes
    aspera, Aloe vera, Azadirachta indica, Datura metal, Hibiscus rosasinensis, Nerium
    oleander, Ocimum sanctum, O. basilicum, Phyllanthus emblica, Polyalthia longifolia, Piper
    betle, Punica granatum, Solanum torvum and Solanum trilobatum were tested in vitro
    against the growth of citrus canker disease causing pathogen, Xanthomonas axonopodis
    pv. citri (Xac) .. The leaf extract of P. longifolia followed by A. aspera recorded the
    maximum growth inhibition of 9.77mm and 8.47mm diameter respectively. The
    20-40% ammonium sulphate fraction of A. aspera and 40-60% fraction of P. longifolia
    exhibited inhibitory action on in vitro growth of the pathogen. The molecular
    weight of the fractions of leaf extracts was found to be 30 kDa and 19 kDa for
    P. long;folia and A. aspera respectively.

    REFERENCES

    1. Balasaraswathy, R (1995). Ph.D. Thesis, Tamil Nadu Agri. Univ., Coimbatore, India.
    2. Bradford, M.M. (1976). Anal. Biochem., 72:248-254.
    3. Chandramohan, B. and Moses, G.J. (1989). In: Seminar on Diseases of Fruit Crops. Tirupati. 81.
    4. Harini, SA, (2000). M.Sc., (Ag.)Thesis, Tamil Nadu Agri. Uni., Coimbatore, India.
    5. Kodama, K. et al., (1991). Int. Symp. Tea Sci. (ISTS):294-298.
    6. Laemmli, U.K. (1970). Nature, 227:680-685.
    7. Majd, A. and Mehrabian, S. (2002). In: XXVI Int. Horti. Congress, Toronto, Canada, August, 11-17.
    8. Muraro, RP. (1986). Food and Resource Econ. Dep., Institute of Food and Agri. Sci., Uni. Florida. Staff Paper 289.
    9. Premananth, K. (1997). M.Sc. (Ag.) Thesis, Tamil Nadu Agri. Univ., Coimbatore, Tamil Nadu, India.
    10. Pruvost,o. etal., (2002). Phytopathology, 92:336-346.
    11. Rajeswari, E. (1991). M.Sc. (Ag.) Thesis, Tamil Nadu Agri. Univ., Coimbatore, India.
    12. Shekawat, P.S. and Prasad, R (1971). Indian Phytopath., 24:800-802.
    13. Sivasankar, A. (1994). M.Sc. (Ag.) Thesis, Tamil Nadu Agri. Univ., Coimbatore, India.
    14. Smale, B.C. and Keil, H.C. (1966). Phytochemistry, 5:1113-1120.
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