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Current IssueEditorial BoardOnline First ArticlesArchive

volume 45 issue 2 (june 2011) : 128- 133

INFLUENCE OF PHOSPHATE SOLUBILIZING PSEUDOMONAS AND BACILLUS STRAINS ON THE GROWTH OF ASHVAGANDHA (Withania somnifera)

M
Mohinder Kaur
S
Sapna Sharma
A
Atul Mishra
1Department of Basic Sciences Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni - 173 230, India.

  • Submitted|

  • First Online |

  • doi

Cite article:- Kaur Mohinder, Sharma Sapna, Mishra Atul (2025). INFLUENCE OF PHOSPHATE SOLUBILIZING PSEUDOMONAS AND BACILLUS STRAINS ON THE GROWTH OF ASHVAGANDHA (Withania somnifera) . Indian Journal of Agricultural Research. 45(2): 128- 133. doi: .

ABSTRACT

Twenty seven bacterial strains of both Pseudomonas and Bacillus sp. from the rhizosphere of Ashvagandha were isolated and screened out for phosphate solubilization and production of other types of plant growth promoting activities affecting directly or indirectly. Out of twenty seven strains six strains , three of Bacillus sp. (WsNB-2, WsNB-6, and WsNB-13) and three of Pseudomonas sp. (WsCP-1, WsCP-5 and WsCP-6) were selected to study the effect of phosphate solubilizing strains on the growth of rooted cutting of Ashvagandha and on other soil parameters like root colonization and phosphorous content of soil before and after the experiment. The selected strains significantly enhanced plant height after two months of plantation. Rhizosphere bacterial population increased significantly, which has resulted in nutrient mobilization and ultimately resulted in enhanced plant growth.

REFERENCES

  1. Bray, R.H. and L.T. Kurtz. (1945). Determination of total organic available forms of phosphorous in soils. Soil Sci. 23: 343-353
  2. Dickman, S.R. and R.H. Bray. (1940). Colorimetric determination of phosphate. Indus and Engineering chemical Analytical Edition 12: 660-665.
  3. Fleming, H.P, Etchelis, J.L. and Costilov, R.H. (1975). Microbial inhibition by an isolate of Pediococcus from cucumber brines. Applied Microbiol., 30: 1040-1042.
  4. Gordan, S.A. and Weber, L.G. (1957). Quantitative measurement of IAA. Physiologia Plantarum, 10: 37-48.
  5. Gupta, M, Kaur, M. and Gupta, K.G.(1992). Lytic effect of Bacillus subtilis elastase on Gram positive and Gram negative bacteria Industrial Journal of Experimental Biology, 30: 380-383.
  6. Gupta, R.P, Singh, P. and Pandher, M.S. (1998). Role of phosphorous solubilizing microorganisms in P-economy and crop yield. In: Soil Plant Microbe Interaction in Relation to Integrated nutrient Management (BD Kaushik ed). IARI, New Delhi, pp.95-101.
  7. Kapoor, K.K, Mishra, M.M. and Kukreja, K. (1989). Phosphate solubilization by soil microorganisms- a review. Indian J. Microbiol., 29: 119-127.
  8. Kaur, M, Gupta M, Tripathi K.K. and Gupta K.G. (1989). Lytic effect of Pseudomonas aeruginosa elastase on Gram positive and Gram negative bacteria. Zentera Blatt bakt-Intern. J. Microbiol., 271(2): 153-157.
  9. Kundu ,B.S. and Gaur, A.C.(1984). J. Plant soil, 79: 227-234.
  10. Lata (2003). Characterization of plant growth promoting rhizobacteria.In: Training Manual on Biofertilizers Technology, (Saxena A.K. ed.), IARI, New Delhi, pp. 24-25.
  11. Oleson, S.R, Cole, C.V, Wahatanable, F.S, and Dean, L.A. (1954). Estimation of available phosphorous by extraction with sodium bicarbonate.USDA Circ. 939.
  12. Payne, S.M. (1994).Detection, isolation and characterization of siderophores. Methods in Enzymol., 235: 329-331.
  13. Pikovskaya, R.E. (1948). Mobilization of phosphorous in soil in connection with vital activity of some microbial species. Mikrobiologia, 17: 362-370.
  14. Rodriguez, H and Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv., 17: 319-339.
  15. Schwyn, B. and Neilands, J.B. (1987). Universal chemical assay for the detection and determination of siderophores. Analytic Biochemistry, 150: 47-56.
  16. Singh, G and Tilak, KVBR. (2001). Phosphorous nutrition through combined inoculation with phosphorous solubilizing rhizobacteria and arbuscular mycorrhiza. Fertilizer News, 46(9): 33-36.
  17. Tandon, H.L.S. (1987). Phosphorous research and agricultural production in India. Fertilizer development and consultation organization, New Delhi, India.
  18. Yadav, KS and Dadarwal, KR. (1997). Phosphate solubilizing and mobilization through soil microorganisms. In: Biotechnological approaches in soil microorganisms for sustainable crop production (KR Dadarwal ed.) Scientific Publisher, Jodhpur p.293-308.
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    volume 45 issue 2 (june 2011) : 128- 133

    INFLUENCE OF PHOSPHATE SOLUBILIZING PSEUDOMONAS AND BACILLUS STRAINS ON THE GROWTH OF ASHVAGANDHA (Withania somnifera)

    M
    Mohinder Kaur
    S
    Sapna Sharma
    A
    Atul Mishra
    1Department of Basic Sciences Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni - 173 230, India.

    • Submitted|

    • First Online |

    • doi

    Cite article:- Kaur Mohinder, Sharma Sapna, Mishra Atul (2025). INFLUENCE OF PHOSPHATE SOLUBILIZING PSEUDOMONAS AND BACILLUS STRAINS ON THE GROWTH OF ASHVAGANDHA (Withania somnifera) . Indian Journal of Agricultural Research. 45(2): 128- 133. doi: .

    ABSTRACT

    Twenty seven bacterial strains of both Pseudomonas and Bacillus sp. from the rhizosphere of Ashvagandha were isolated and screened out for phosphate solubilization and production of other types of plant growth promoting activities affecting directly or indirectly. Out of twenty seven strains six strains , three of Bacillus sp. (WsNB-2, WsNB-6, and WsNB-13) and three of Pseudomonas sp. (WsCP-1, WsCP-5 and WsCP-6) were selected to study the effect of phosphate solubilizing strains on the growth of rooted cutting of Ashvagandha and on other soil parameters like root colonization and phosphorous content of soil before and after the experiment. The selected strains significantly enhanced plant height after two months of plantation. Rhizosphere bacterial population increased significantly, which has resulted in nutrient mobilization and ultimately resulted in enhanced plant growth.

    REFERENCES

    1. Bray, R.H. and L.T. Kurtz. (1945). Determination of total organic available forms of phosphorous in soils. Soil Sci. 23: 343-353
    2. Dickman, S.R. and R.H. Bray. (1940). Colorimetric determination of phosphate. Indus and Engineering chemical Analytical Edition 12: 660-665.
    3. Fleming, H.P, Etchelis, J.L. and Costilov, R.H. (1975). Microbial inhibition by an isolate of Pediococcus from cucumber brines. Applied Microbiol., 30: 1040-1042.
    4. Gordan, S.A. and Weber, L.G. (1957). Quantitative measurement of IAA. Physiologia Plantarum, 10: 37-48.
    5. Gupta, M, Kaur, M. and Gupta, K.G.(1992). Lytic effect of Bacillus subtilis elastase on Gram positive and Gram negative bacteria Industrial Journal of Experimental Biology, 30: 380-383.
    6. Gupta, R.P, Singh, P. and Pandher, M.S. (1998). Role of phosphorous solubilizing microorganisms in P-economy and crop yield. In: Soil Plant Microbe Interaction in Relation to Integrated nutrient Management (BD Kaushik ed). IARI, New Delhi, pp.95-101.
    7. Kapoor, K.K, Mishra, M.M. and Kukreja, K. (1989). Phosphate solubilization by soil microorganisms- a review. Indian J. Microbiol., 29: 119-127.
    8. Kaur, M, Gupta M, Tripathi K.K. and Gupta K.G. (1989). Lytic effect of Pseudomonas aeruginosa elastase on Gram positive and Gram negative bacteria. Zentera Blatt bakt-Intern. J. Microbiol., 271(2): 153-157.
    9. Kundu ,B.S. and Gaur, A.C.(1984). J. Plant soil, 79: 227-234.
    10. Lata (2003). Characterization of plant growth promoting rhizobacteria.In: Training Manual on Biofertilizers Technology, (Saxena A.K. ed.), IARI, New Delhi, pp. 24-25.
    11. Oleson, S.R, Cole, C.V, Wahatanable, F.S, and Dean, L.A. (1954). Estimation of available phosphorous by extraction with sodium bicarbonate.USDA Circ. 939.
    12. Payne, S.M. (1994).Detection, isolation and characterization of siderophores. Methods in Enzymol., 235: 329-331.
    13. Pikovskaya, R.E. (1948). Mobilization of phosphorous in soil in connection with vital activity of some microbial species. Mikrobiologia, 17: 362-370.
    14. Rodriguez, H and Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv., 17: 319-339.
    15. Schwyn, B. and Neilands, J.B. (1987). Universal chemical assay for the detection and determination of siderophores. Analytic Biochemistry, 150: 47-56.
    16. Singh, G and Tilak, KVBR. (2001). Phosphorous nutrition through combined inoculation with phosphorous solubilizing rhizobacteria and arbuscular mycorrhiza. Fertilizer News, 46(9): 33-36.
    17. Tandon, H.L.S. (1987). Phosphorous research and agricultural production in India. Fertilizer development and consultation organization, New Delhi, India.
    18. Yadav, KS and Dadarwal, KR. (1997). Phosphate solubilizing and mobilization through soil microorganisms. In: Biotechnological approaches in soil microorganisms for sustainable crop production (KR Dadarwal ed.) Scientific Publisher, Jodhpur p.293-308.
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