Indian Journal of Agricultural Research

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Research Article

Indian Journal of Agricultural Research, volume 55 issue 4 (august 2021) : 493-496

Effect of the Different Concentrations of Protective-stimulating Complex (HFC) on the Growth and Development of Grape Seedlings in the Tissue Culture at the Different Nutrient Levels

Esraa M.M. Farahat, S.L. Belopukhov
1Department of Chemistry, Russian State Agrarian University - MTAA named after K.A. Timiryazev, 127550, Timiryazevsky proezd, 2, Moscow, Russian Federation.
Cite article:- Farahat M.M. Esraa, Belopukhov S.L. (2021). Effect of the Different Concentrations of Protective-stimulating Complex (HFC) on the Growth and Development of Grape Seedlings in the Tissue Culture at the Different Nutrient Levels. Indian Journal of Agricultural Research. 55(4): 493-496. doi: 10.18805/IJARe.A-613.

ABSTRACT

Background: Humic substances plays a vital role in the plant tissue culture as a growth hormone for in vitro propagation of many plant seedlings. The aim of this study was to investigate the effect of added humic-fulvate complex (HFC) at the various concentrations on the growth and development of grape seedlings in in vitro at the different nutrient levels.  
Methods: The cutting of khasansky grape were cultivated on ¼ Murashige and Skoog medium or ½ Murashige and Skoog medium either alone or supplemented with the humic-fulvate complex at the different concentrations at (0.1, 1 and 10 ml/l). Then, they were cultured for 4 weeks under a controlled environment. 
Result: The data observed that the low concentration of Murashige and Skoog medium (¼ MS) for in vitro rooting of grape cv. ‘Khasansky’ either alone or combined with HFC at the various concentrations significantly increased the rooting percentages and the total length of roots and stimulating the rate of vegetative growth compared with cultivated in ½ MS medium either alone or with supplemented with HFC. ¼ MS+ 10 ml/l HFC was the best treatment for improving the growth of khasansky grape seedlings.

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