Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

  • NAAS Rating 5.60

  • SJR 0.293

Frequency :
Bi-monthly (February, April, June, August, October and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Agricultural Research, volume 55 issue 5 (october 2021) : 535-541

Results of the Biochemical Study of Mandarin (Citrus reticulata Blanco) Mutants

G.R. Memarne, N.I. Khalvashi, M.T. Gabaidze, D. Sh. Baratashvili, A.R. Kalandia, M.G. Vanidze, I.O. Kartsivadze
1Institute of Phytopathology and Biodiversity, Batumi Shota Rustaveli State University, Georgia.
Cite article:- Memarne G.R., Khalvashi N.I., Gabaidze M.T., Baratashvili Sh. D., Kalandia A.R., Vanidze M.G., Kartsivadze I.O. (2021). Results of the Biochemical Study of Mandarin (Citrus reticulata Blanco) Mutants. Indian Journal of Agricultural Research. 55(5): 535-541. doi: 10.18805/IJARe.A-591.

ABSTRACT

Background: Induced mutagenesis has been used in citrus breeding, in Georgia, since 1960, resulting in many interesting and promising mutant forms that require morphological, physiological and biochemical studies. The present paper presents the results of biochemical research of some promising mutants. 
Methods: During the period 2017-2019 investigate on 13 promising mutant forms of mandarin has been carried out. In the current study, phenolic composition content in the fruits of mandarin mutants were determined using Ultraviolet Spectrophotometer and High Performance Liquid Chromatography (HPLC) and their antioxidant capacities were evaluated using 2.2-diphenyl-1-picrylhydrazyl (DPPH) radicals.
Result: The results have shown that different concentrations of mutagen effect on mandarin seeds and, accordingly, on the biochemical components which they include. In most cases, this leads to a positive result. The impact of the mutagen significantly increases the range of changes in biochemical components and varies over a wide range. Analyzing the data, we can conclude that the solutions of all concentrations of the Nash mutagen effect a change in the biochemical indicators in mutant forms. However, a wide range of variability is largely associated with the use of medium and low concentrations of the mutagen. In most cases, the majority of mutant forms significantly outperform the control variants in the content of biochemical components. The study has revealed a variety of mutant forms (gene pool), which characterized a whole complex of positive signs. Their use in selection allows getting perspective forms with positive features.

REFERENCES

  1. Bermejo, A., Llosa, M. and Cano, A. (2011). Analysis of Bioactive Compounds in Seven Citrus Cultivars. Food Science and Technology. 17(1): 55-62. 
  2. Cameron, J.W. and Frost, H.B. (1968). Genetics, Breeding and Nucellar Embryonic. Berkeley, CA: California. pp. 47-59.
  3. Cottin, R. (2002). Citrus of the World. A Citrus Directory. SRA INRA CIRAD, San Giuliano. Version 2: pp. 62.
  4. Del Caro Alessandra, Piga Antonio, Vacca Vincenzo and Agabbio Mario (2004). Changes of flavonoids, vitamin C and antioxidant capacity in minimally processed citrus segments and juices during storage. Food Chemistry. 84: 99-105.
  5. Diasamidze, A.O. (1994). Natural and Chemical mutagenesis in citrus breeding. Publishing Adjara, pp. 131.
  6. Gil-Izquierdo, A., Gil, M.I., Ferreres, F. and Tomas-Barberan, F. (2001). In vitro availability of flavonoids and other phenolics in orange juice. Journal of Agriculture and Food Chemistry. 49: 1035-1041.
  7. Goliadze, S.K. (1989). Chemical Mutagenesis of Citrus Breeding. Soviet Adjara. pp. 105.
  8. Kerkadze, I.G. (1984). Induced mutation in subtropical groups. Aspects of the effects of chemical mutagens on the somatic tissues of citrus. Subtropical. Kulture. 5: 54-62. 
  9. Maluszynski, M., Nichterlein, K., Van Zanten and Ahloowalia B.S. (2000). Officially released mu­tant varieties. Plant Breeding and Genetics Section. 12: 1-11. 
  10. Masayoshi Sawamura, Nguyen Minh Tu, Yuji Onishi, Eriko Ogawa and Hyang-Sook Choi (2004). Characteristic Odor Components of Citrus reticulata Blanco Cold-pressed Oil. Bioscience, Biotechnology and Biochemistry. 68(8): 1690-1697. 
  11. Micke, A.B., Donini, M. and Maluszynski, M. (1987). Induced mutations for crop impro­ve­m­ent review. Tropical Agriculture. 64: 259-272.
  12. Okava, M., Kinjo, J., Nohara, T. and Ono, M. (2001). DPPH (1.1-Diphenyl-2-Pierylhdrazyl) Ra-Dical Scavenging activity of flavonoids Obtained from Some Medical Plants. Biological and Pharmaceutical Bulletin. 24: 1202-1204.
  13. Peterson, J.J., Beecher, G.R., Bhagwat, S.A., Dwyer, J.T., Gebhardt, S.E., Haytowitz, D.B. and Holden, J.M. (2006). Flavanones in grapefruit, lemons and limes. Journal of Food Composition and Analysis. 19: 74-80.
  14. Sutarto, I.D., Agisimanto, D. and Supriyanto, A. (2009). Development of promising seedless citrus mutants through gamma irradiation. Induced Plant Mutations in the Genomics Era. 42: 306-308.
  15. Tanaka, T. (1925). Further data on bud-variation in Citrus. Japanese Journal of Genetics. 3(3): 131-143.
  16. Tomlekova, N.B. (2010). Induced Mutagenesis for Crop Improvement in Bulgaria. Plant Mutation Reports. 2: 4-28.
  17. Watada, A.E., Abe, K. and Yamauchi, N. (1990). Physiological activities of partially processed fruits and vegetables. Food Technology. 20: 120-122. 
  18. Zoz, N.N. (1968). Mutation breeding. pp. 217. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)