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Research Article

volume 54 issue 4 (august 2020) : 511-515,   Doi: 10.18805/IJARe.A-5262

Genotypic Analysis for Retrieval of MYMV Resistant Progenies from Certain Crosses of Mungbean [Vigna radiata (L.) Wilczek]

V
V. Sandhiya
S
S. Saravanan
1Department of Plant Breeding and Genetics Agricultural College and Research Institute, Killikulam. 
Cite article:- Sandhiya V., Saravanan S. (2020). Genotypic Analysis for Retrieval of MYMV Resistant Progenies from Certain Crosses of Mungbean [Vigna radiata (L.) Wilczek]. Indian Journal of Agricultural Research. 54(4): 511-515. doi: 10.18805/IJARe.A-5262.

ABSTRACT

The present investigation was conducted to study the nature of inheritance of MYMV among intra-specific crosses among three MYMV susceptible and five MYMV resistant genotypes of green gram. The molecular screening and hotspot evaluation is carried out in fifteen F1 progenies and 8 F2 families, respectively. The yellow mosaic-affected leaf samples gave positive results with MYMIV specific primer pairs YFP1/YRP1 and YFP2/YRP2 amplified fragments having size of 704bp and 648 bp, respectively. Coat protein gene sequence analysis revealed that the identified virus is homologous to MYMV. The inheritance study based on F2 data indicated the ratio of MYMV resistant plants and susceptible plants showed a good fit to expected 3:1 (Susceptible: Resistant) suggesting a typical monogenic recessive gene governing resistant reaction to MYMV besides resting the susceptibility from the susceptible parents.

REFERENCES

  1. Balakrishna, J., Hari, Y and Thirumala Rao, V. (2019). Genetic diversity analysis among Yellow Mosaic Virus (YMV) resistant and susceptible varieties in mung bean (Vigna radiata L.) using SSR markers. J Food Process Technol. 10. DOI: 10.4172/2157-7110-C1-111.
  2. Bhattacharya, A. and Vijaylaxmi, A. (2005). Genetic diversity in mungbean: phenological, physiological and yield forming traits. Legume Research. 28:1-6.
  3. Gautam, N. K., Akram, M., Akhtar, J., Khan, Z., Dwivedi, N.K., Latha, M and Ram, B. (2014). Responses of wild Vigna species/    sub-species to yellow mosaic disease viruses, detected by a PCR-based method. Phytopathologia Mediterranea. 53(3): 428-437.
  4. Joshi, M. and Deshpande, J. D. (2010). Polymerase Chain Reaction: Methods, Principles and Application. Int. J. Biomedical Research. 1(5): 81 97.
  5. Karthikeyan, A., Shobhana, V.G., Sudha, M., Raveendran, M., Senthil, N. Pandiyan, M and Nagarajan, P. (2014). Mungbean yellow mosaic virus (MYMV): A threat to greengram (Vigna radiata) productionin Asia. Int. J. Pest Management. 60(4): 15-19.
  6. Khajudparn, P., Poolsawat, O and Tantasawat, P.A. (2019). Genetic studies in mungbean [Vigna radiata (L.) Wilczek]. Legume Research. 42:42-54.
  7. Khan, M.G., Ahmad, W., Khatta,G. S. S., Din, S. and Ahmad, H. (2007). Mode of inheritance of resistance to mungbean yellow mosaic virus (MYMV) in mungbean [Vigna radiate (L.) Wilczek]. Sarhad J. Agric. 23(4): 1071-1074.
  8. Khattak, G. S. S., Haq, M. A., Ashraf, M and Elahi., T. (2000). Genetics of Mungbean. Yellow mosaic Virus (MYMV) in mungbean    [Vigna radiata (L.) Wilczek.]. J. Genet. and Breed. 54: 237-243.
  9. Kumar, P. Sreenivasulu, L. P. and Waliyar, F. (2007). Methods manual Diagnosis and Detection of viruses Infecting ICRISAT Mandate Crops. ICRISAT News. 2 (7): 39-49.
  10. Lazarowitz, S. and Shepherd, R.J. (1992). Gemini viruses: genome structure and gene function. Crit. Rev. Plant Sci. 11: 327-349.
  11. Maheshwari, R., Panigrahi, G. and Angappan, K. (2014). Molecular characterization of distinct YMV (Yellow mosaic virus) isolates affecting pulses in India with the aid of coat protein gene as a marker for identification. Mol. Biol. Rep. 41: 2635–2644.
  12. Manjunatha, N., Haveri, N., Reddy, B.A., Archana, S. and Hurakadli, S. (2015). Molecular Detection and Characterization of Virus Causing Yellow Mosaic Disease of Redgram [Cajanus cajan (L.) Millsp] in Karnataka. Int. J. Pure App. Biosci. 3 (4): 258-264.
  13. Marabi, R.S., Deepti Sagare, B., Das, S.B., Bhowmick, A. K. and Noda, H. (2017). Molecular detection of Mungbean Yellow Mosaic India Virus (MYMIV) infecting soybean in Madhya Pradesh. Biosci. Biotech. Res. Asia. 14(1): 315-318.
  14. Narasimhulu, R., Naidu, N.V. and Reddy, K.H.P. (2016). Genetic analysis for yield and yield attributes in greengram [Vigna radiata (L.) Wilczek]. Legume Research. 41:349-355. DOI: 10.18805/LR-3768.
  15. Nene, Y. L. (1972). A Survey of the Viral Disease of Pulse Crops in Uttar Pradesh. Research Bulletin, G.B. Pant Univ. Agric. Tech., Pantnagar. 191-192.
  16. Nene, Y. L. (1988). Multiple disease resistance in grain legumes. Annual review of Phyto-Pathology. 26 (1): 203-217.
  17. Qazi, J., Ilyas, M., Mansoor, S., Briddon, R. (2007). Legume yellow mosaic viruses: Genetically isolated begomoviruses. Molecular plant pathology. 8. 343-348. 
  18. Rani, A., Kumar, V., Gill, B.S., Rathi, P., Shukla, S., Singh, R.K. and Husain, S.M. (2017). Linkage mapping of Mungbean Yellow Mosaic India Virus (MYMIV) resistance gene in soybean. Breed. Sci. 67: 95-100.
  19. Rathi, Y.P.S. (2002). Epidemeology, yield losses and management of major diseases of Kharif pulses in India. In. Plant pathology and Asian congress of mycology and plant pathology. Oct. 1-4. University of Mysore, Mysore. India.
  20. Raturi, A., Singh S.K., Sharma V and Pathak R. (2015). Genetic variability, heritability, genetic advance and path analysis in mungbean [Vigna radiata (L.) Wilczek]. Legume Research. 38(2):157-163.
  21. Singh G., Kapoor, S. and Singh, K. (1988). Multiple disease resistance in mungbean with special emphasis on Mungbean yellow mosaic virus In: Int. Symposium on Mungbean, 2nd Nov 16-20 (1987) Bangkok., Thailand: 290- 296.
  22. Singh, B. B. (2011). Project Coordinators Report. Indian Council of AgriculturalResearch. Indian Institute of Pulses Research. All India Coordinated Research Project on MULLaRP. Annual Group Meet. 11-13.
  23. Singh, D. P. (1980). Inheritance of resistance to mungbean yellow mosaic virus in blackgram, Theor. Appl. Genet. 57: 233-235.
  24. Singh, J. and Mishra, K.K. (2016). Viral diseases of Mungbean and their management. In book: Indian Agriculture and Farmers, Publisher: Poddar Publication, Tara Nagar, Chittupur, BHU, Varanasi-221 005. 197-205.
  25. Subhash, C., Rajvanshi, N.K., Kumar, P., Kumar, M. and Chauhan, M.P. (2019). Screening of mungbean [Vigna radiata (L.) Wilczek] genotypes for resistance against mungbean yellow mosaic virus (MYMV) under field condition. International Journal of Chemical Studies. 7(3): 469-47.
  26. Sudha, M., Karthikeyan, A., Anusuya, P., Ganesh, N. M., Pandiyan, M., Senthil, N., Raveendran, M., Nagarajan, P., Angappan, K. (2013). Inheritance of resistance to Mungbean Yellow Mosaic Virus (MYMV) in Inter and Intra -specific crosses of Mungbean (Vigna radiata). American. J. Pl. Sci. 4:1924-    1927.
  27. Vinod, J., Dhole and Reddy, K.S. (2013). Development of a SCAR marker linked with a MYMV resistance gene in mungbean [Vigna radiata (L.) wilczek]. Pl. Breed. 132: 127-132.
  28. Webster, C. G., Wylie, S.J. and Jones, M.G.K. (2004). Diagnosis of plant viral pathogens. Current Sci. 86(12): 1604-1607.
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    Research Article

    volume 54 issue 4 (august 2020) : 511-515,   Doi: 10.18805/IJARe.A-5262

    Genotypic Analysis for Retrieval of MYMV Resistant Progenies from Certain Crosses of Mungbean [Vigna radiata (L.) Wilczek]

    V
    V. Sandhiya
    S
    S. Saravanan
    1Department of Plant Breeding and Genetics Agricultural College and Research Institute, Killikulam. 
    Cite article:- Sandhiya V., Saravanan S. (2020). Genotypic Analysis for Retrieval of MYMV Resistant Progenies from Certain Crosses of Mungbean [Vigna radiata (L.) Wilczek]. Indian Journal of Agricultural Research. 54(4): 511-515. doi: 10.18805/IJARe.A-5262.

    ABSTRACT

    The present investigation was conducted to study the nature of inheritance of MYMV among intra-specific crosses among three MYMV susceptible and five MYMV resistant genotypes of green gram. The molecular screening and hotspot evaluation is carried out in fifteen F1 progenies and 8 F2 families, respectively. The yellow mosaic-affected leaf samples gave positive results with MYMIV specific primer pairs YFP1/YRP1 and YFP2/YRP2 amplified fragments having size of 704bp and 648 bp, respectively. Coat protein gene sequence analysis revealed that the identified virus is homologous to MYMV. The inheritance study based on F2 data indicated the ratio of MYMV resistant plants and susceptible plants showed a good fit to expected 3:1 (Susceptible: Resistant) suggesting a typical monogenic recessive gene governing resistant reaction to MYMV besides resting the susceptibility from the susceptible parents.

    REFERENCES

    1. Balakrishna, J., Hari, Y and Thirumala Rao, V. (2019). Genetic diversity analysis among Yellow Mosaic Virus (YMV) resistant and susceptible varieties in mung bean (Vigna radiata L.) using SSR markers. J Food Process Technol. 10. DOI: 10.4172/2157-7110-C1-111.
    2. Bhattacharya, A. and Vijaylaxmi, A. (2005). Genetic diversity in mungbean: phenological, physiological and yield forming traits. Legume Research. 28:1-6.
    3. Gautam, N. K., Akram, M., Akhtar, J., Khan, Z., Dwivedi, N.K., Latha, M and Ram, B. (2014). Responses of wild Vigna species/    sub-species to yellow mosaic disease viruses, detected by a PCR-based method. Phytopathologia Mediterranea. 53(3): 428-437.
    4. Joshi, M. and Deshpande, J. D. (2010). Polymerase Chain Reaction: Methods, Principles and Application. Int. J. Biomedical Research. 1(5): 81 97.
    5. Karthikeyan, A., Shobhana, V.G., Sudha, M., Raveendran, M., Senthil, N. Pandiyan, M and Nagarajan, P. (2014). Mungbean yellow mosaic virus (MYMV): A threat to greengram (Vigna radiata) productionin Asia. Int. J. Pest Management. 60(4): 15-19.
    6. Khajudparn, P., Poolsawat, O and Tantasawat, P.A. (2019). Genetic studies in mungbean [Vigna radiata (L.) Wilczek]. Legume Research. 42:42-54.
    7. Khan, M.G., Ahmad, W., Khatta,G. S. S., Din, S. and Ahmad, H. (2007). Mode of inheritance of resistance to mungbean yellow mosaic virus (MYMV) in mungbean [Vigna radiate (L.) Wilczek]. Sarhad J. Agric. 23(4): 1071-1074.
    8. Khattak, G. S. S., Haq, M. A., Ashraf, M and Elahi., T. (2000). Genetics of Mungbean. Yellow mosaic Virus (MYMV) in mungbean    [Vigna radiata (L.) Wilczek.]. J. Genet. and Breed. 54: 237-243.
    9. Kumar, P. Sreenivasulu, L. P. and Waliyar, F. (2007). Methods manual Diagnosis and Detection of viruses Infecting ICRISAT Mandate Crops. ICRISAT News. 2 (7): 39-49.
    10. Lazarowitz, S. and Shepherd, R.J. (1992). Gemini viruses: genome structure and gene function. Crit. Rev. Plant Sci. 11: 327-349.
    11. Maheshwari, R., Panigrahi, G. and Angappan, K. (2014). Molecular characterization of distinct YMV (Yellow mosaic virus) isolates affecting pulses in India with the aid of coat protein gene as a marker for identification. Mol. Biol. Rep. 41: 2635–2644.
    12. Manjunatha, N., Haveri, N., Reddy, B.A., Archana, S. and Hurakadli, S. (2015). Molecular Detection and Characterization of Virus Causing Yellow Mosaic Disease of Redgram [Cajanus cajan (L.) Millsp] in Karnataka. Int. J. Pure App. Biosci. 3 (4): 258-264.
    13. Marabi, R.S., Deepti Sagare, B., Das, S.B., Bhowmick, A. K. and Noda, H. (2017). Molecular detection of Mungbean Yellow Mosaic India Virus (MYMIV) infecting soybean in Madhya Pradesh. Biosci. Biotech. Res. Asia. 14(1): 315-318.
    14. Narasimhulu, R., Naidu, N.V. and Reddy, K.H.P. (2016). Genetic analysis for yield and yield attributes in greengram [Vigna radiata (L.) Wilczek]. Legume Research. 41:349-355. DOI: 10.18805/LR-3768.
    15. Nene, Y. L. (1972). A Survey of the Viral Disease of Pulse Crops in Uttar Pradesh. Research Bulletin, G.B. Pant Univ. Agric. Tech., Pantnagar. 191-192.
    16. Nene, Y. L. (1988). Multiple disease resistance in grain legumes. Annual review of Phyto-Pathology. 26 (1): 203-217.
    17. Qazi, J., Ilyas, M., Mansoor, S., Briddon, R. (2007). Legume yellow mosaic viruses: Genetically isolated begomoviruses. Molecular plant pathology. 8. 343-348. 
    18. Rani, A., Kumar, V., Gill, B.S., Rathi, P., Shukla, S., Singh, R.K. and Husain, S.M. (2017). Linkage mapping of Mungbean Yellow Mosaic India Virus (MYMIV) resistance gene in soybean. Breed. Sci. 67: 95-100.
    19. Rathi, Y.P.S. (2002). Epidemeology, yield losses and management of major diseases of Kharif pulses in India. In. Plant pathology and Asian congress of mycology and plant pathology. Oct. 1-4. University of Mysore, Mysore. India.
    20. Raturi, A., Singh S.K., Sharma V and Pathak R. (2015). Genetic variability, heritability, genetic advance and path analysis in mungbean [Vigna radiata (L.) Wilczek]. Legume Research. 38(2):157-163.
    21. Singh G., Kapoor, S. and Singh, K. (1988). Multiple disease resistance in mungbean with special emphasis on Mungbean yellow mosaic virus In: Int. Symposium on Mungbean, 2nd Nov 16-20 (1987) Bangkok., Thailand: 290- 296.
    22. Singh, B. B. (2011). Project Coordinators Report. Indian Council of AgriculturalResearch. Indian Institute of Pulses Research. All India Coordinated Research Project on MULLaRP. Annual Group Meet. 11-13.
    23. Singh, D. P. (1980). Inheritance of resistance to mungbean yellow mosaic virus in blackgram, Theor. Appl. Genet. 57: 233-235.
    24. Singh, J. and Mishra, K.K. (2016). Viral diseases of Mungbean and their management. In book: Indian Agriculture and Farmers, Publisher: Poddar Publication, Tara Nagar, Chittupur, BHU, Varanasi-221 005. 197-205.
    25. Subhash, C., Rajvanshi, N.K., Kumar, P., Kumar, M. and Chauhan, M.P. (2019). Screening of mungbean [Vigna radiata (L.) Wilczek] genotypes for resistance against mungbean yellow mosaic virus (MYMV) under field condition. International Journal of Chemical Studies. 7(3): 469-47.
    26. Sudha, M., Karthikeyan, A., Anusuya, P., Ganesh, N. M., Pandiyan, M., Senthil, N., Raveendran, M., Nagarajan, P., Angappan, K. (2013). Inheritance of resistance to Mungbean Yellow Mosaic Virus (MYMV) in Inter and Intra -specific crosses of Mungbean (Vigna radiata). American. J. Pl. Sci. 4:1924-    1927.
    27. Vinod, J., Dhole and Reddy, K.S. (2013). Development of a SCAR marker linked with a MYMV resistance gene in mungbean [Vigna radiata (L.) wilczek]. Pl. Breed. 132: 127-132.
    28. Webster, C. G., Wylie, S.J. and Jones, M.G.K. (2004). Diagnosis of plant viral pathogens. Current Sci. 86(12): 1604-1607.
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