Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

  • NAAS Rating 5.60

  • SJR 0.293

Frequency :
Bi-monthly (February, April, June, August, October and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Agricultural Research, volume 55 issue 3 (june 2021) : 289-295

Phytochemical Characteristics Evaluation of Pleurotus species Cultivated on Agricultural Wastes in Chiro, Ethiopia

Getachew Gashaw, Abebe Getu
1Department of Biology, College of Natural and Computational Sciences Oda Bultum University, Chiro, P.O. Box: 226; Ethiopia.
Cite article:- Gashaw Getachew, Getu Abebe (2021). Phytochemical Characteristics Evaluation of Pleurotus species Cultivated on Agricultural Wastes in Chiro, Ethiopia. Indian Journal of Agricultural Research. 55(3): 289-295. doi: 10.18805/IJARe.A-526.
Background: Mushrooms are a nutritious food source, being rich in protein, vitamins and minerals. They are also contains substances that enhance the immune system, fight infectious disease. Mushrooms can be cultivated on a variety of substrates, including agricultural and agro-industrial waste materials. The current study was amid to evaluate the phytochemical characteristics of Pleurotus species cultivated on different agricultural wastes.
Methods: Mushrooms Pleurotus ostreatus and Pleurotus florida were cultivated on different agricultural wastes for the screening of phytochemical characteristics. Qualitative analyses of the phytochemicals were evaluated in methanolic, ethanolic and aqueous extracts of both Pleurotus spp. Total phenolic and total flavonoid contents of the extracts were determined by using Folin-Ciocalteu method and Spectrophotometric method with aluminum chloride.
Result: Qualitative analyses revealed the phytochemicals alkaloids, saponins, flavonoids and tannins were present in methanolic, ethanolic and aqueous extracts of both Pleurotus spp. while anthraquinones and Phlobatannins were absent in aqueous extracts. The highest concentration of phenols and flavonoids were recorded in methanolic extracts of P. ostreatus and P. florida (48.17 mg GAE/g of extract and 56.57 mg of RUE/g of extract and 46.73 mg GAE/g of extract and 55.58 mg of RUE/g of extract respectively). The results supported the methanolic extracts of P. ostreatus and P. florida might indeed be potential sources of phytochemicals. 
  1. Arvouet-Grand, A., Vennat, B., Pourrat, A. and Legret, P. (1994). Standardization d’un extrait de propolis et identification des principaux constituants. Journal de Pharmacie de Belgique. 49: 462-468.
  2. Azieana, J., Zainon, M.N., Noriham, A. and Rohana, M.N. (2017). Total phenolic and flavonoid content and antioxidant activities of ten Malaysian wild mushrooms. Open Access Library Journal. 4: e3987.
  3. Aziz T, Mehmet E.D. and Nazime A.M. (2007). Antioxidant and Antimicrobial Activity of Russula delica Fr: An Edi Wild Mus. Eur. J. of Ana. Chem. 2: 64-7. 
  4. Bano, Z. and Srivastava, H.C. (1962). Studies in the cultivation of Pleurotus sp. on paddy straw. Food Sci. 12: 363-368. 
  5. Barros, L., Baptista, P., Estevinho, L.M. and Ferreira, I.C. (2007). Effect of fruiting body maturity stage on chemical composition and antimicrobial activity of Lactarius sp. mushrooms. J. Agric. Food Chem. 55: 8766-71. 
  6. Čanadanović-Brunet, J., Ćetković, G., Đilas, S., Tumbas, V., Bogdanović, G., Mandić, A., Markov, S., Cvetković, D. and Čanadanović, V. (2008). Radical scavenging, antibacterial and anti-proliferative activities of Melissa officinalis L. extracts. J. Med. Food. 11: 133-143.
  7. Chung, K.T. (1998). Department of Microbiology and Molecular cell sciences university of Memphis, TN 38152, USA. 38(6): 421-64. 
  8. Fai-Chu W., Tsun-Thai C., Soon-Leong T. and Ann-Li Y. (2013). Evaluation of Bioactivities and Phenolic Content of Selected Edible Mushrooms in Malaysia. Tropical Journal of Pharmaceutical Research. 12(6): 1011-1016. 
  9. Gan, C.H., Nurul-Amira, B. and Asmah, R. (2013). Antioxidant analysis of different types of edible mushrooms (Agaricus bisporous and Agaricus brasiliensis). International Food Research Journal. 20(3): 1095-1102.
  10. Gregori, A., Svagel, M. and Pohleven, J. (2007). Cultivation techniques and medicinal properties of Pleurotus spp. Food Technol. Biotechnol. 45: 238-249.
  11. Harborne, J.B. and Williams, C.A. (2001). Anthocyanins, other flavonoids. Nat prod. Rep. 18: 310-333. 
  12. Harborne, J.B. (1973). Phytochemical Methods. Chapman and Hall Ltd., London. 11-113.
  13. Imran M.M., Raja, M.M., Basith, M.A. and Asarudin A. (2011). Determination of total phenol, flavanoid and antioxidant activity of edible mushrooms Pleurotus florida and Pleurotus eous. Inter. Food Res J. 18: 574-577.
  14. Jayakumar, T., Thomas, P.A. and Geraldine, P. (2009). In vitro antioxidant activities of an ethanolic extract of the oyster mushroom, Pleurotus ostreatus. Innovative Food Sci. Emerging Technol. 10: 228-234.
  15. Jonathan, G. (2012). Antagonistic effect of extracts of some Nigerian higher fungi against selected pathogenic microorganisms. American-Eurasian J. Agric. and Environ. Sci. 4: 364-368.
  16. Jyoti T., Shrayanee D., Zeeshan F. and Saif, H. (2014). Multidrug Resistance: An Emerging Crisis. Interdisciplinary Perspectives on Infectious Diseases. Hindawi Publishing Corporation, Volume 2014, Article ID 541340, 7 Pages.
  17. Karuppusamy, S. (2009). A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. J. Med. Plants Res. 3: 1222-1239.
  18. Kérley B.B.C., Maria C. M. K. and Maria C.D.V. (2011). Antimicrobial activity and mineral composition of Shiitake mushrooms cultivated on agricultural waste. Braz. Arch. Biol. Technol. 54(5): 991-1002.
  19. Kim D.O., Jeong S.W., Lee C.Y. (2003). Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem. 81: 321-326.
  20. Komal, K.N. Shashitha, Kavitha. G. Singh and Shlini. P. (2016). Utilization of vegetable waste for the cultivation of P. ostreatus and its influence on phytochemicals. Cibtech Journal of Bio-Protocols, 5(2): 22-29.
  21. Kong, J.M., Goh, N.K., Chia, L.S. and Chia, T.F. (2003). Recent advances in traditional plants drugs and orchids. Acta Pharmacologia Sci. 24: 7-21. 
  22. Kumar, R., Sharma, R.J., Bairwa, K., Roy, R.K. and Kumar, A. (2010). Pharmacological review on natural antidiarrhoel agents. Der Pharma Chemical. 2(2): 66-93. 
  23. Lesney, M.S. (2004). Nature’s Pharmaceuticals: Natural Products from Plants Remain at the Core of Modern Medicinal Chemistry. TCAW. 13(7): 26-31. 
  24. Luís, A., Domingues, F., Gil, C. and Duarte, A.P. (2009). Antioxidant activity of extracts of Portuguese shrubs: Pterospartum tridentatum, Cytisus scoparius and Erica spp. J. Med. Plants Res. 3(11): 886-893.
  25. Marijana, K., Branislav, R. and Marko, D. (2012). Mushrooms as possible antioxidant and antimicrobial agents. Iran J. Pharm. Res. 11(4): 1095-1102.
  26. Martinez-valverde and Periago, M.J. (2000). Unidad Docente de bromatologia. inspeccion de Alimentos, Faculted de veterinaria, Universidad de Murcia, Espana. Arch Latinoam Nutr. 50(1): 5-18. 
  27. Mena Ritota and Pamela Manzi. (2019). Pleurotus spp. Cultivation on Different Agri-Food By-Products: Example of Biotechnological Application. Review Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA) Centro di ricerca Alimenti Nutrizione via Ardeatina 546, 00178 Rome, Italy.
  28. Menaga, D., Mahalingam, P.U., Rajakumar, S. and Ayyasamy, P.M. (2012). Evaluation of phytochemical characteristics and antimicrobial activity of Pleurotus florida mushroom. Asian J. Pharm. Clin. Res. 5(4): 102-106.
  29. Min, G. and Chun-Zhao, L. (2005). Comparison of techniques for the extraction of flavonoids from cultured cells of Saussurea medusa Maxim. World J. Microb. Biot. 21: 1461-1463.
  30. Mohsen, M.S., Ammar, S.M.A. (2008). Total phenolic contents and antioxidant activity of corn tassel extracts. Food Chem. 112: 595-598.
  31. Newman, D.J., Cragg, G.M. and Snader, K.M. (2003). Natural products as sources of new drugs over the period 1981-2002. J. Natural Products. 66: 1022-1032. 
  32. Okwulehie, I.C. and Ogoke J.A. (2013). Bioactive, nutritional and heavy metal constituents of some edible mushrooms found in Abia State of Nigeria. Int. J. Appl. Microbiol. Biotechnol. Res. 1(2): 7-15.
  33. Padmavathy, S. and Mekala, V. (2013). Preliminary phytochemical investigation of some medicinal plants of western ghats, The Nilgiris. Int. J. Res. in Pharmaceutical and Biomed. Sci. 4(1).
  34. Pushpa, H. and Purushothama, K.B. (2010). Antimicrobial activity of Lyophyllum decastes an edible wild mushroom. World Journal of Agricultural Sciences. 6(5): 506-509. 
  35. Sharififar, F., Nudeh-Dehghn, G. and Mirtajaldini, M. (2008). Major flavonoids with antioxidant activity from Teucrium polium L. Food Chem. 112: 885-888.
  36. Tosun, M., Ercisli, S., Sengul, M., Ozer, H. and Polat, T. (2009). Antioxidant properties and total phenolic content of eight Salvia species from Turkey. Biol. Res. 41: 175-181.
  37. Trease, G.E. and Evans, W.C. (1994). Pharmacognosy XII Ed. London, Bailere London.

Editorial Board

View all (0)