Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

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  • SJR 0.293

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Indian Journal of Agricultural Research, volume 54 issue 3 (june 2020) : 361-366

Embryogenic Capabilities of Cotton through Tissue Culture and Expression Analysis of SERK3 for Regeneration Potential

Mizna, Moon Sajid, Sameera Hassan, Bushra Rashid, Tayyab Husnain
1Centre of Excellence in Molecular Biology, University of the Punjab, 87-W Canal Bank Road Thokar Niaz Baig, Lahore-53700, Pakistan.
Cite article:- Mizna, Sajid Moon, Hassan Sameera, Rashid Bushra, Husnain Tayyab (2020). Embryogenic Capabilities of Cotton through Tissue Culture and Expression Analysis of SERK3 for Regeneration Potential. Indian Journal of Agricultural Research. 54(3): 361-366. doi: 10.18805/IJARe.A-515.
Plant tissue culture technique was used to develop a regeneration procedure in local Pakistani cotton varieties. Three cotton varieties Coker 312, CEMB66 and Klean cotton were cultured on MS media supplemented with vitamins and growth regulators. The results showed that the regeneration potential of Coker 312 was higher, CEMB 66 moderate while Klean cotton lacks the cotton regeneration potential. The molecular analysis identified the Somatic Embryogenesis Receptor Kinase3 (SERK3) gene and its expression analysis confirmed that it plays role for embryo formation through tissue culture. Its low expression resulted in calli to die rather than forming embryos. Therefore, SERK3 gene is considered important for callus induction and its development into primary embryogenic and embryogenic stage. Further investigations will be helpful to understand the mechanism of regeneration potential of cotton through tissue culture.
  1. Ahsan, M., Majeedano, M., Channa, A., Panhwar, F.H., Soomro, A.W., Khaskheli, F., Rashid, K. (2014). Regeneration of cotton (Gossypium L.) through asexual methods, a review. American-Eurasian Journal of Agriculture and Environmental Sciences. 14: 1478-1486. 
  2. Anandan, R., Deenathayalan, T., Bhuvaneshwari, R., Monisha, M.M., Prakash, M. (2019). An efficient protocol for rapid plant regeneration from deembryonated cotyledons of black gram (Vigna mungo L. cv Hepper). Indian Journal of Agricultural Research. 53(5): 589-593.
  3. Ashwini P.B., Shelke, P., Mahajan, V. (2018). Three step protocol for regeneration of plantlets in Indian garlic varieties using root meristem. Indian Journal of Agricultural Research. 52(1): 66-70
  4. Chaudhary, G. and Dantu, K. (2015). Evaluation of callus browning and develop a strategically callus culturing of Boerhaavia diffusa L. Journal of Plant Development. 22: 47-58.
  5. Cheng, G.W. and Crisosto, C.H. (1995). Browning potential, phenolic composition and polyphenooxidase activity of buffer extracts of peach and nectarine skin tissue. Journal of the American Society for Horticultural Science. 120: 835-838.
  6. Cousins, Y.L., Lyon, B.R., Llewellyn, D.J. (1991). Transformation of an Australian cotton cultivar: Prospects for cotton through genetic engineering. Australian Journal of Plant Physiology. 18: 481-494.
  7. Dev, S.S., Poornima P., Venu, A. (2018). Molecular characterization and phylogenetic analysis of NBS-LRR genes in wild relatives of eggplant (Solanum melongena L.). Indian Journal of Agricultural Research. 52(2): 167-171
  8. Fernández, F., Gutiérrez, J., Sorlózano, A., Romero, J.M., Soto, M.J., Ruiz-Cabello, F. (2006). Comparison of the SYBR Green and the hybridization probe format for real-time PCR detection of HHV-6. Microbiological Research. 161(2): 158-163
  9. Gamborg, C. Miller, A, Ojima, K. (1968). Nutrients requirements of suspension cultures of soybean root cells. Experimental Cell Research. 50(1): 151-158.
  10. Haq, I. and Yusuf, Z. (2004). Effect of nitrates on embryo induction efficiency in cotton (Gossypium L.). African Journal of Biotechnology. 3: 319-323.
  11. Jaakola, L., Pirttilä, A.M., Halonen, M., Hohtola, A. (2001). Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit. Molecular Biotechnology. 19(2): 201-203.
  12. Khan, T., Singh, A., Pant, R. (2006). Regeneration via somatic embryoenesis and organogenesis in different cultivars of cotton (Gossypium SPP.). In Vitro Cellular and Developmental Biology - Plant. 42: 498-501. 
  13. Kumar, A., Priya, Sharma, S., Yadav, M.K. (2019). Plant tissue culture technology to improve crop species-a comprehensive approach. Acta Scientific Agriculture. 13(2): 76-80.
  14. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
  15. Pilarska, M., Malec, P., Salaj, J., Bartnicki, F., Konieczny, R. (2016). High expression of Somatic Embryogenesis Receptor-    Like Kinase coincides with initiation of various developmental pathways in invitro culture of Trifolium nigrescens. Protoplasma. 253(2): 345–355.
  16. Rangan, T.S. and Rajasekaran, K. (1996) Regeneration of cotton plants in suspension culture. USA patent. 5: 583, 036.
  17. Rashid, B., Husnain, T., Riazuddin, S. (2004). In vitro shoot tip culture of cotton (Gossypium hirsutum). Pakistan Journal of Botany. 36(4) :817-823
  18. Smith, R.H., Price, H.J., Thaxton, J.B. (1977). Defined conditions for the initiation on growth of cotton callus in vitro I.Gossypium arboreum. In Vitro. 13: 329-334.
  19. Sparjanbabu, D.S., Naveen Kumar, P., Krishna, M.S.R., Ramajayam, D., Kalyana, B.B., Susanthi, B.(2019). Effect of culture media, plant growth regulators and genotypes on growth and developmental stages of oil palm (Elaeis guineensis Jacq.) zygotic embryos. Indian Journal of Agricultural Research. 53(2): 143-150.
  20. Trolinder, N.L. and Goodin, J.R. (1987). Somatic embryogenesis and plant regeneration in cotton (Gossypium L.). Plant Cell Reports. 6: 231–234.
  21. Trolinder, N.L. and Goodin, J.R. (1988a). Somatic embryogenesis in cotton (Gossypium) I. Effects of source of explant and hormone regime. Plant Cell Tissue and Organ Culture. 12: 178-181.
  22. Trolinder, N.L. and Goodin, J.R. (1988b). Somatic embryogenesis in cotton (Gossypium) II. Requirements for embryo development and plant regeneration. Plant Cell Tissue and Organ Culture. 12 : 43-53.
  23. Yang, X. and Zhang, X. (2010). Regulation of somatic embryogenesis in higher plants. Critical Reviews in Plant Science. 29(1): 36-57.
  24. Zhang, B.H., Feng, R., Liu. F., Wang, Q. (2001). High frequency somatic embryogenesis and plant regeneration of an elite Chinese cotton variety. Botanical Bulletin of Academia Sinica. 42: 9-16.
  25. Zhang, T., Qian, N., Zhu, X., Chen, H., Wang, S., Mei, H., Zhang, Y. (2013). Variations and transmission of QTL alleles for yield and fiber qualities in upland cotton cultivars developed in China. Plos one. 8(2): e57220.

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