Banner
Current IssueEditorial BoardOnline First Articles
Current IssueEditorial BoardOnline First Articles
Indian Journal of
Agricultural Research
Print ISSN: 0367-8245
Online ISSN: 0976-058X
NASS Rating
5.60
SJR
0.217
CiteScore
0.595

Chief Editor:
V. Geethalakshmi
Tamil Nadu Agricultural University Coimbatore, INDIA
Frequency:Monthly
Indexing:
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Go...

Research Article

Indian Journal of Agricultural Research, volume 53 issue 1 (february 2019) : 8-16

Morphological and molecular genetic diversity analysis using SSR markers in Jackfruit (Artocarpus heterophyllus Lam.) genotypes for pulp colour
 

K. Kavya, S. Shyamalamma, S. Gayatri
1Advanced Centre for Plant Biotechnology, University of Agricultural Sciences, GKVK, Bangalore-560 065, Karnataka, India.
Cite article:- Kavya K., Shyamalamma S., Gayatri S. (2019). Morphological and molecular genetic diversity analysis using SSR markers in Jackfruit (Artocarpus heterophyllus Lam.) genotypes for pulp colour. Indian Journal of Agricultural Research. 53(1): 8-16. doi: 10.18805/IJARe.A-5066.

ABSTRACT

Jackfruit (Artocarpus heterophyllus Lam.) being an important dryland horticultural tree, grows well with minimum care and maintenance. The study aimed at identifying the genetic diversity present in twenty jackfruit genotypes for different pulp colours. The genotypes were identified for commercial cultivation, based on fruit and flake quality characters viz., fruit weight, fruit rind weight per kg fruit, number of flakes per kg fruit, weight of flakes per kg fruit, individual fresh flake weight and TSS as per the Jackfruit descriptors given by IPGRI, Rome. The best genotypes identified from the study were Swarna, Lalbagh Madhura, Byrachandra, NSP, Ashoka Yellow and NEL and these can be used for commercial purpose. Molecular diversity analysis was also carried out using 22 SSR primers, out of which six primers (SSR 9, SSR 10, SSR 30, SSR 34, SSR 45 and SSR 48) showed polymorphism among twenty genotypes. The genetic similarity co-efficient ranged from 0 to 0.96, indicating a vast variation in genetic diversity for pulp colour. A dendrogram was constructed by UPGMA analysis revealed three major clusters. The genotypes with cream and yellow colour pulp grouped in cluster I and II, followed by yellow, orange and red in cluster III. These markers effectively segregated the genotypes based on different pulp colours and hence, can be used for both diversity analysis and in breeding applications.

REFERENCES

  1. Ambade, R.L., Verma, S.K., Nanda, H.C., Nair, S.K., and Verulkar, S.B. (2015). Genetic diversity based on molecular markers in grasspea (Lathyrus sativus L.). Legume Res. 38(1): 43-46.
  2. Aranzana, M., Pineda, A., Cosson, P., Dirlewanger, E., Ascasibar, J., Cipriani, G. and Iezzoni, A. (2003). A set of simple-sequence repeat (SSR) markers covering the Prunus genome. Theor. Appl. Genet. 106(5): 819-825.
  3. Azad, A.K., Jones, J.G. and Haq, N. (2007) Assessing morphological and isozyme variation of Jackfruit (Artocarpus heterophyllus Lam.) in Bangladesh. Agrofor. Syst., 71: 109-125.
  4. Barkley, N.A, Roose, M.L., Krueger, R.R. and Federici C.T. (2006). Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs), Theor. Appl. Genet., 112: 1519–1531.
  5. Burkill, H.M. (1997). The Useful Plants of West Tropical Africa. Ann. Roy. Bot. Gard., 2: 2.
  6. Eiadthong W., Yonemori, K., Sugiura, A., Utsunomiya N. and Subhadrabandhu S. (1999). Identification of mango cultivars of Thailand and evaluation of their genetic variation using the amplified fragments by simple sequence repeat-(SSR-) anchored primers, Sci. Horti.., 82: 57-66.
  7. Guruprasad, T.R. (1981). Studies on systematic selection of jackfruit (Artocarpus heterophyllus Lam.) types. M. Sc. (Hort.) Thesis.
  8. Hashmi, S.I. and Pawar, V.N. (2012). Studies of physical and chemical characteristics of Custard apple fruit pulp from different locations. J. Dairying, Foods & H.S., 31(2): 117-120.
  9. Jagadeesh, S.L., Reddy, B.S., Swamy, G.S.K., Gorbal, K., Hegde,L., and Raghavan, G.S.V. (2007). Chemical composition of Jackfruit (Artocarpus heterophyllus Lam.) selections of Western Ghats of India. Food Chem., 102(1): 361-365.
  10. Krishnan, A.G., Sabu, T.S., Sible, G.V., and Xavier, L. (2015). Genetic diversity analysis in Jackfruit selections of Kuttanad region using RAPD technique. Int. J. Sci. Res, 5(4): 1-6.
  11. Padmakar, B., Kanupriya, C., Latha, P. M., Prashant, K.S., Dinesh, M. R., Sailaja, D. and Aswath, C. (2015). Development of SRAP and SSR marker-based genetic linkage maps of guava (Psidium guajava L.), Sci. Horti. 192: 158–165.
  12. Petchiammal, K.I., Muthiah, A.R. and Jayamani, P. (2015). Molecular characterization of cultivated and wild Cajanus species using Simple Sequence Repeat markers. Legume Res., 38(6): 742-747.
  13. Pushpakumara, D.K.N.G., and Harris, S.A. (2007). Potential of RAPD markers for identification of fruit types of Artocarpus. J. Natl. Sci. Found. 35(3).
  14. Rahman, M.A., Nahar, N., Mian, A.J. and Mosihuzzaman, M. (1999). Variation of carbohydrate composition of two forms of fruit from jack tree (Artocarpus heterophyllus L.) with maturity and climatic conditions, Food chem., 65: 91-97.
  15. Reddy, B.M.C., Patil, P., Shashikumar, S., Govindaraju, L.R. (2004). Studies on physico-chemical characteristics of Jackfruit clones of south Karnataka. Karnataka J. Agric. Sci., 17(2): 279–282.
  16. Rohlf, F.J. (2000). NTSYS-pc Numerical taxonomy and multivariate analysis system, version 2.01. Setauket New York: Appl. Biostatistics. 
  17. Rowe-Dutton, P., Garner, R.J., and Chaudhri, S.A. (1985). Artocarpus heterophyllus-Jackfruit. The propagation of tropical fruit trees. 269–290.
  18. Samaddar, H.N. (1985). Jackfruit. Fruits of India: Tropical and subtropical, 487-497.
  19. Shyamalamma, S., Chandra, S.B.C., Hegde, M., and Naryanswamy, P. (2008). Evaluation of genetic diversity in jackfruit (Artocarpus heterophyllus Lam.) based on amplified fragment length polymorphism markers. Genet. Mol. Res., 7(3), 645-656.
  20. Shyamalamma, S., Vinitha, P.A., Priyanka, S. and Ramanjinigowda, P.H. (2015). Comparitive study of carotenoids and mineral composition in five different pulp colours of Jackfruit (Artocarpus heterophyllus Lam.). Int. J. Sci. Res., 5(9): 1380-1383.
  21. Singh, J., Dhall, R.K., and Aulakh, P.S. (2011). Molecular characterization of Biodiversity in Vegetables- A review. Agri. Reviews., 32(3): 193-201.
  22. Singh, S.R., Narayanaswamy, P., Yumnam, S.S., and Banik, B.C. (2011). Development of RAPD marker related to fruit cracking in jackfruit (Artocarpus heterophyllus Lam). Asian J. Hort., 6(1), 199-202.
  23. Sneath, P.H., and Sokal, R.R. (1973). Numerical taxonomy. The principles and practice of numerical classification. 
  24. Uma, M.S., Hegde, N., and Hittalmani, S. (2016). Identification of SSR marker associated with rust resistance in cowpea (Vigna unguiculata L.) usi ng bulk segregant analysis. Legume Res., 39(1), 39-42.
  25. Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., and Tingey, S.V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic acids Res., 18(22): 6531-6535.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)