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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 4 (august 2018) : 355-361,   Doi: 10.18805/IJARe.A-5035

Isolation, characterization and growth response study of chlorpyrifos utilizing soil bacterium Pseudomonas putida JR16

J
Jalpa K. Rank
N
Neelam M. Nathani
A
Ankit T. Hinsu
A
Anjali U. Joshi
M
Mootapally Chandra Shekar
R
Ramesh K. Kothari
Cite article:- Rank K. Jalpa, Nathani M. Neelam, Hinsu T. Ankit, Joshi U. Anjali, Shekar Chandra Mootapally, Kothari K. Ramesh (2018). Isolation, characterization and growth response study of chlorpyrifos utilizing soil bacterium Pseudomonas putida JR16. Indian Journal of Agricultural Research. 52(4): 355-361. doi: 10.18805/IJARe.A-5035.

ABSTRACT

Agricultural pesticides are one of the indispensable materials used to meet increasing demand of food crops. Consequently, pesticides find their way to various ecosystems resulting in detrimental pollution problems. Chlorpyrifos is a predominantly used organophosphorus pesticide leading to harmful effects including abnormal cell division. Scientific attempt to eliminate these contaminants from the environment using biodegradation approach has been appreciated. Soil bacteria capable of utilizing chlorpyrifos are potential bioremediation candidates. In context to the same, the present study isolated six bacteria growing in presence of chlorpyrifos as sole carbon source from agricultural soils. The obtained bacterial isolates characterized based on their morphological characteristics, biochemical reactions were found to be Pseudomonas spp. JR16, Pseudomonas spp. J1, Pseudomonas spp. J2, Pantoea spp. J3, Enterobacter spp. J4 and Kocuria spp. J5. Among these, the most potential of them viz., Pseudomonas spp. JR16, was further proceeded for molecular identification and was observed to be the  Pseudomonas putida JR16. The influence of different chlorpyrifos concentration, various carbon sources, metals sources, temperature and pH on the growth of Pseudomonas putida JR16 was assessed for developing optimum conditions for on field application of the strain for biodegradation of chlorpyrifos.

REFERENCES

  1. Abo-Amer, A., (2011). Biodegradation of diazinon by Serratiamarcescens DI 101 and its use in bioremediation of contaminated environment. Journal of Microbiology Biotechnology. 21: 71-80. 
  2. Agu, K.C., Orji, M.U., Onuorah, S.C., Egurefa, S.O., (2014). Influence of solid waste dumps leachate on bacteriological and heavy metals contamination of ground water in Awka. Am. J. Life. Sci. Res., 2: 450-457.
  3. Akbar, S., Sultan, S., (2016). Soil bacteria showing a potential of chlorpyrifos degradation and plant growth enhancement. Braz. J. Microbiol., 47: 563-570. 
  4. Cho, C.M.H., Mulchandani, A., Chen, W., (2002). Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agents. Appl. Environ. Microbiol., 68: 2026-2030. 
  5. Chu, X.Y., Wu, N.F., Deng, M.J., Tian, J., (2006). Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: Purification and characterization. Protein Expr. Purif., 49: 9-14. 
  6. Fulekar, M., Geetha, M., (2008). Bioremediation of chlorpyrifos by Pseudomonas aeruginosa using scale up technique. J. Appl. Biosci., 12: 657-660. 
  7. Gupta, S.K., Gupta, S., Rajwade, J., Panigrahy, K., Shinde, K.P., (2017). Effect and control of pesticide toxicity on agriculture, animals and human health : A review. Bhartiya Krishi Anushandhan Patrika, 32: 115-118.
  8. Kumar, S., (2011). Bioremediation of chlorpyrifos by bacteria isolated from the cultivated soil. Int. J. Pharma. Biosci., 2, 359-366. 
  9. Kunadia, K., Nathani, N.M., Kothari, V.K., Kotadiya, R., Kothari, C.R., Joshi, A., et al (2016). Draft Genome Sequence of Commercial Textile Dye-Decolorizing and Degrading Bacillus subtilis Strain C3 Isolated in India. Genome Announcements., 4: 14-16.
  10. Kurhade, K.C., Gade, R.M., Belkar, Y.K., Chaitanya, B.H., (2016). Detecting tolerance in Pseudomonas fluorescens to pesticides. Agric. Sci. Digest., 36: 247-249.
  11. Li, X., He, J., Li, S., (2007). Isolation of a chlorpyrifos-degrading bacterium, Sphingomonas sp. strain Dsp-2, and cloning of the mpd gene. Res. Microbiol., 158: 143-149. 
  12. Olson, G.J., Brierley, J.A., Brierley, C.L., (2003). Bioleaching review part B. Appl. Microbiol. Biotechnol., 63: 249-257. 
  13. Patel, P.A., Kothari, V.V., Kothari, C.R., Faldu, P.R., (2014). Draft Genome Sequence of Petroleum Hydrocarbon-Degrading Pseudomonas aeruginosa Strain PK6, isolated from the Saurashtra Region of Gujarat, India. Genome Announc., 2. 
  14. Ramanathan, M.P., and Lalithakumari, D., (1999). Complete mineralization of methylparathion by Pseudomonas sp. A3. Appl. Biochem. Biotechnol., 80: 1-12. 
  15. Shafiani, S., and Malik, A., (2003). Tolerance of pesticides and antibiotic resistance in bacteria isolated from wastewater-irrigated soil. World. J. Microbiol. Biotechnol., 19: 897-901. 
  16. Singh, B.K., Walker, A., Morgan, J.A., Wright, D.J., (2004). Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Appli. Environ. Microbiol., 70: 4855-4863. 
  17. Singh, B.K., Walker, A., Morgan, J.A.W., Wright, D.J., (2003). Effects of soil pH on the biodegradation of chlorpyrifos and isolation of a chlorpyrifos-degrading bacterium. Appl. Environ. Microbiol., 69: 5198-5206. 
  18. Surekha, R.M., Vijaya, L.K., Suvarnalatha, D.P., Jaya, M.R., (2008). Isolation and characterization of a chlorpyrifos-degrading bacterium from agricultural soil and its growth response. Afr. J. Microbiol. Res., 2: 26-31. 
  19. Visalakshi, A., Mohmamed, A.B., Rema, D.L., Mohandas, N., (1980). The effect of carbofuran on the rhizosphere microflora of rice. J. Microbiol., 20: 147–148. 
  20. Xu, G., Zheng, W., Yingying, Li., Wang, S., (2008). Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by a newly isolated Paracoccus sp. strain TRP. Int. Biodeterior Biodegradation, 62: 51-56. 
  21. Yadav, M., Srivastva, N., Singh, R.S., Upadhyay, S.N., (2014). Biodegradation of chlorpyrifos by Pseudomonas sp. in a continuous packed bed bioreactor. Bioresource Technol., 165: 265-269. 
  22. Yang, C., Liu, N., Guo, X., Qiao, C., (2006). Cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in bioremediation of contaminated soil. FEMS Microbiol. Lett., 265: 118-125. 
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Indian Journal of Agricultural Research
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    Research Article

    volume 52 issue 4 (august 2018) : 355-361,   Doi: 10.18805/IJARe.A-5035

    Isolation, characterization and growth response study of chlorpyrifos utilizing soil bacterium Pseudomonas putida JR16

    J
    Jalpa K. Rank
    N
    Neelam M. Nathani
    A
    Ankit T. Hinsu
    A
    Anjali U. Joshi
    M
    Mootapally Chandra Shekar
    R
    Ramesh K. Kothari
    Cite article:- Rank K. Jalpa, Nathani M. Neelam, Hinsu T. Ankit, Joshi U. Anjali, Shekar Chandra Mootapally, Kothari K. Ramesh (2018). Isolation, characterization and growth response study of chlorpyrifos utilizing soil bacterium Pseudomonas putida JR16. Indian Journal of Agricultural Research. 52(4): 355-361. doi: 10.18805/IJARe.A-5035.

    ABSTRACT

    Agricultural pesticides are one of the indispensable materials used to meet increasing demand of food crops. Consequently, pesticides find their way to various ecosystems resulting in detrimental pollution problems. Chlorpyrifos is a predominantly used organophosphorus pesticide leading to harmful effects including abnormal cell division. Scientific attempt to eliminate these contaminants from the environment using biodegradation approach has been appreciated. Soil bacteria capable of utilizing chlorpyrifos are potential bioremediation candidates. In context to the same, the present study isolated six bacteria growing in presence of chlorpyrifos as sole carbon source from agricultural soils. The obtained bacterial isolates characterized based on their morphological characteristics, biochemical reactions were found to be Pseudomonas spp. JR16, Pseudomonas spp. J1, Pseudomonas spp. J2, Pantoea spp. J3, Enterobacter spp. J4 and Kocuria spp. J5. Among these, the most potential of them viz., Pseudomonas spp. JR16, was further proceeded for molecular identification and was observed to be the  Pseudomonas putida JR16. The influence of different chlorpyrifos concentration, various carbon sources, metals sources, temperature and pH on the growth of Pseudomonas putida JR16 was assessed for developing optimum conditions for on field application of the strain for biodegradation of chlorpyrifos.

    REFERENCES

    1. Abo-Amer, A., (2011). Biodegradation of diazinon by Serratiamarcescens DI 101 and its use in bioremediation of contaminated environment. Journal of Microbiology Biotechnology. 21: 71-80. 
    2. Agu, K.C., Orji, M.U., Onuorah, S.C., Egurefa, S.O., (2014). Influence of solid waste dumps leachate on bacteriological and heavy metals contamination of ground water in Awka. Am. J. Life. Sci. Res., 2: 450-457.
    3. Akbar, S., Sultan, S., (2016). Soil bacteria showing a potential of chlorpyrifos degradation and plant growth enhancement. Braz. J. Microbiol., 47: 563-570. 
    4. Cho, C.M.H., Mulchandani, A., Chen, W., (2002). Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agents. Appl. Environ. Microbiol., 68: 2026-2030. 
    5. Chu, X.Y., Wu, N.F., Deng, M.J., Tian, J., (2006). Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: Purification and characterization. Protein Expr. Purif., 49: 9-14. 
    6. Fulekar, M., Geetha, M., (2008). Bioremediation of chlorpyrifos by Pseudomonas aeruginosa using scale up technique. J. Appl. Biosci., 12: 657-660. 
    7. Gupta, S.K., Gupta, S., Rajwade, J., Panigrahy, K., Shinde, K.P., (2017). Effect and control of pesticide toxicity on agriculture, animals and human health : A review. Bhartiya Krishi Anushandhan Patrika, 32: 115-118.
    8. Kumar, S., (2011). Bioremediation of chlorpyrifos by bacteria isolated from the cultivated soil. Int. J. Pharma. Biosci., 2, 359-366. 
    9. Kunadia, K., Nathani, N.M., Kothari, V.K., Kotadiya, R., Kothari, C.R., Joshi, A., et al (2016). Draft Genome Sequence of Commercial Textile Dye-Decolorizing and Degrading Bacillus subtilis Strain C3 Isolated in India. Genome Announcements., 4: 14-16.
    10. Kurhade, K.C., Gade, R.M., Belkar, Y.K., Chaitanya, B.H., (2016). Detecting tolerance in Pseudomonas fluorescens to pesticides. Agric. Sci. Digest., 36: 247-249.
    11. Li, X., He, J., Li, S., (2007). Isolation of a chlorpyrifos-degrading bacterium, Sphingomonas sp. strain Dsp-2, and cloning of the mpd gene. Res. Microbiol., 158: 143-149. 
    12. Olson, G.J., Brierley, J.A., Brierley, C.L., (2003). Bioleaching review part B. Appl. Microbiol. Biotechnol., 63: 249-257. 
    13. Patel, P.A., Kothari, V.V., Kothari, C.R., Faldu, P.R., (2014). Draft Genome Sequence of Petroleum Hydrocarbon-Degrading Pseudomonas aeruginosa Strain PK6, isolated from the Saurashtra Region of Gujarat, India. Genome Announc., 2. 
    14. Ramanathan, M.P., and Lalithakumari, D., (1999). Complete mineralization of methylparathion by Pseudomonas sp. A3. Appl. Biochem. Biotechnol., 80: 1-12. 
    15. Shafiani, S., and Malik, A., (2003). Tolerance of pesticides and antibiotic resistance in bacteria isolated from wastewater-irrigated soil. World. J. Microbiol. Biotechnol., 19: 897-901. 
    16. Singh, B.K., Walker, A., Morgan, J.A., Wright, D.J., (2004). Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Appli. Environ. Microbiol., 70: 4855-4863. 
    17. Singh, B.K., Walker, A., Morgan, J.A.W., Wright, D.J., (2003). Effects of soil pH on the biodegradation of chlorpyrifos and isolation of a chlorpyrifos-degrading bacterium. Appl. Environ. Microbiol., 69: 5198-5206. 
    18. Surekha, R.M., Vijaya, L.K., Suvarnalatha, D.P., Jaya, M.R., (2008). Isolation and characterization of a chlorpyrifos-degrading bacterium from agricultural soil and its growth response. Afr. J. Microbiol. Res., 2: 26-31. 
    19. Visalakshi, A., Mohmamed, A.B., Rema, D.L., Mohandas, N., (1980). The effect of carbofuran on the rhizosphere microflora of rice. J. Microbiol., 20: 147–148. 
    20. Xu, G., Zheng, W., Yingying, Li., Wang, S., (2008). Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by a newly isolated Paracoccus sp. strain TRP. Int. Biodeterior Biodegradation, 62: 51-56. 
    21. Yadav, M., Srivastva, N., Singh, R.S., Upadhyay, S.N., (2014). Biodegradation of chlorpyrifos by Pseudomonas sp. in a continuous packed bed bioreactor. Bioresource Technol., 165: 265-269. 
    22. Yang, C., Liu, N., Guo, X., Qiao, C., (2006). Cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in bioremediation of contaminated soil. FEMS Microbiol. Lett., 265: 118-125. 
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