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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 2 (april 2019) : 202-207,   Doi: 10.18805/IJARe.A-5020

In vitro propagation of Coccinia indica (L.) Voigt. from internodal segments.

A
Ani Rani Borah
J
J. Anbumalarmathi
A
Aruna Sharmili S.
1Department of Biotechnology, Stella Maris College (Autonomous), Chennai-600 086, Tamil Nadu, India.
Cite article:- Borah Rani Ani, Anbumalarmathi J., S. Sharmili Aruna (2019). In vitro propagation of Coccinia indica (L.) Voigt. from internodal segments.. Indian Journal of Agricultural Research. 53(2): 202-207. doi: 10.18805/IJARe.A-5020.

ABSTRACT

An efficient, rapid, reproducible and prolific protocol has been established for in vitro plant propagation via direct and indirect regeneration from internodal explants of Coccinia indica. The direct and indirect regeneration capability of internodal segments was studied on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins and auxins either alone or in combinations. In direct regeneration, the highest regeneration percentage (90%) with maximum frequency of shoot length (7.2 cm) was achieved on D6: MS + 2.5 mg/L BAP (6-Benzylaminopurine) + 1.0 mg/L Kn (Kinetin). In indirect regeneration, the highest regeneration percentage (91.6%) was achieved on I5: MS + 1.0 mg/L BAP + 0.3 mg/L NAA(1-Napthaleneaceticacid). The highest frequency of shoot induction with shoot number (5) and shoot length (8.52 cm) was achieved on IS2: MS + 1.0 mg/L BAP + 0.5 mg/L Kn. Microshoots obtained best rooting response on IR3: MS + 1.0 mg/L IAA and 0.5 mg/L Kn by producing a highest frequency of root number (4.7) and root length (7.03 cm). The rooted microshoots were successfully hardened and were acclimatized in green house and subsequently established in soil with survival rate of 90%.

REFERENCES

  1. Anita M. (2017) Effect of plant growth regulators on callus multiplication and in vitro plant regeneration in Bacopa monnieri L., International Journal of Medicinal Plants Research, 6(5): 337-345.
  2. Ankita P R and Kaplesh I B. (2015) In vitro Shoot Multiplication from Nodal Explants of Coccinia grandis (L.) Voigt. and its Antidiabetic and Antioxidant Activity. Asian Journal of Biological Sciences. 
  3. Arulanandam L J P and Ghanthikumar S. (2011) Indirect organogenesis of Vitrex trifolia Linn- An important medicinal plant. Indian Journal of Natural Product and Resources, 2(2): 261-264.
  4. Bekele F, Balcha Abera and Mezgebe Getahun (2013). In vitro propagation of Anchote (Coccinia abyssinica) (Lam.) Cogn. African Journal of Plant Science. 7(6): 253-264.
  5. Geoffrey K C, Elaine Porter A, Monique Simmonds S J. (2007) Chromatographic behaviours of steroidal saponins studied by high-    performance liquid chromatography-mass spectrometry. Journal of Chromatography A, 1148: 177-183.
  6. Ghanthikumar S, Arulanandam L J P and Henry Joseph L. (2013) Micropropagation of Coccinia indica Wight & Arn.- A medicinal plant. Int. Applied Bio Research, 18: 1-4.
  7. Han J S, Oh D G, Mok I G, Park H G, Kim C K. (2006) Efficient plant regeneration from cotyledon explants of bottle gourd (Lagneria siceraria Standl.) Plant Cell Reports, 23(5): 291-296.
  8. Narayan J P. (2016) Ex-situ Conservation of the rare and threatened medicinal climber Corallocarpus epigaeus Rottler through In vitro regeneration method. British Biotechnology Journal, 14(3): 1-10.
  9. Rahmatullah Md, Nur Kabidul Azam Md, Zubedah Khatun, Syeda Seraj, Farhana Islam, Atiqur Rahman Md, Sharmina Jahan, Shah Aziz Md. (2012) Medicinal plants used for the treatment of Diabetes by the Marakh Sect of the Garo tribe living in Mymensingh district, Bangladesh. African Network on Ethnomedicines, 9(3): 380-385.
  10. Ramasubbu R and Chandraprabha A. (2014) Micropropagation of Citrullus colocynthis (L.) Schrader: An important antidiabetic Plant. New Horizons in Biotechnology and Bio and medicines, 271-275. 
  11. Randhir Singh, Lalit Kumar, Navpreet Kaur, Samrat Chauhan. (2014) Phyto-Pharmacological Review of Coccinia indica. World Journal of Pharmacy and Pharmaceutical Sciences, 3(2): 1734-1745. 
  12. Sarkar P, FMS, Jahan R. and Rahmatullah M. (2008) In vitro regeneration of Coccinia grandis (L.) Voigt. An indigineous medicinal plant of Bangladesh. Afr. J. Trad. Comp. Alt. Med. 
  13. Senthil Kumar P, Paulsamy S, Vijayakumar K K and Kalimuthu K. (2007) In vitro regeneration of the medicinal herbs of Nilgiri Shola, Acmella calva L. from leaf derived callus. Plant Tissue Culture and Biotechnology, 17(2): 109-114. 
  14. Shekhawat M S, Ravindran C P and Manokari M. (2014) Developmental and Hormonal Regulation of direct shoots and roots regeneration in Coccinia indica. International Journal of Natural Sciences Research, 2(7): 103-112.
  15. Shekhawat M S, Shekhawat M S, Harish R, Kheta M, Phulwaria, and Gupta A K. (2011) High frequency plantlet regeneration from nodal segment culture of female Momordica dioica (Roxb.), Journal Crop Sci. Biotech,14: 133-137.
  16. Shyam Kumar B, Gnanasekaran D, Jaishree V and Channabasavaraj K P. (2010) Hepatoprotective activity of Coccinia indica leaves extract. International Journal of Pharmaceutical and Biomedical Research, 1(4): 154-156.
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Indian Journal of Agricultural Research
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    Research Article

    volume 53 issue 2 (april 2019) : 202-207,   Doi: 10.18805/IJARe.A-5020

    In vitro propagation of Coccinia indica (L.) Voigt. from internodal segments.

    A
    Ani Rani Borah
    J
    J. Anbumalarmathi
    A
    Aruna Sharmili S.
    1Department of Biotechnology, Stella Maris College (Autonomous), Chennai-600 086, Tamil Nadu, India.
    Cite article:- Borah Rani Ani, Anbumalarmathi J., S. Sharmili Aruna (2019). In vitro propagation of Coccinia indica (L.) Voigt. from internodal segments.. Indian Journal of Agricultural Research. 53(2): 202-207. doi: 10.18805/IJARe.A-5020.

    ABSTRACT

    An efficient, rapid, reproducible and prolific protocol has been established for in vitro plant propagation via direct and indirect regeneration from internodal explants of Coccinia indica. The direct and indirect regeneration capability of internodal segments was studied on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins and auxins either alone or in combinations. In direct regeneration, the highest regeneration percentage (90%) with maximum frequency of shoot length (7.2 cm) was achieved on D6: MS + 2.5 mg/L BAP (6-Benzylaminopurine) + 1.0 mg/L Kn (Kinetin). In indirect regeneration, the highest regeneration percentage (91.6%) was achieved on I5: MS + 1.0 mg/L BAP + 0.3 mg/L NAA(1-Napthaleneaceticacid). The highest frequency of shoot induction with shoot number (5) and shoot length (8.52 cm) was achieved on IS2: MS + 1.0 mg/L BAP + 0.5 mg/L Kn. Microshoots obtained best rooting response on IR3: MS + 1.0 mg/L IAA and 0.5 mg/L Kn by producing a highest frequency of root number (4.7) and root length (7.03 cm). The rooted microshoots were successfully hardened and were acclimatized in green house and subsequently established in soil with survival rate of 90%.

    REFERENCES

    1. Anita M. (2017) Effect of plant growth regulators on callus multiplication and in vitro plant regeneration in Bacopa monnieri L., International Journal of Medicinal Plants Research, 6(5): 337-345.
    2. Ankita P R and Kaplesh I B. (2015) In vitro Shoot Multiplication from Nodal Explants of Coccinia grandis (L.) Voigt. and its Antidiabetic and Antioxidant Activity. Asian Journal of Biological Sciences. 
    3. Arulanandam L J P and Ghanthikumar S. (2011) Indirect organogenesis of Vitrex trifolia Linn- An important medicinal plant. Indian Journal of Natural Product and Resources, 2(2): 261-264.
    4. Bekele F, Balcha Abera and Mezgebe Getahun (2013). In vitro propagation of Anchote (Coccinia abyssinica) (Lam.) Cogn. African Journal of Plant Science. 7(6): 253-264.
    5. Geoffrey K C, Elaine Porter A, Monique Simmonds S J. (2007) Chromatographic behaviours of steroidal saponins studied by high-    performance liquid chromatography-mass spectrometry. Journal of Chromatography A, 1148: 177-183.
    6. Ghanthikumar S, Arulanandam L J P and Henry Joseph L. (2013) Micropropagation of Coccinia indica Wight & Arn.- A medicinal plant. Int. Applied Bio Research, 18: 1-4.
    7. Han J S, Oh D G, Mok I G, Park H G, Kim C K. (2006) Efficient plant regeneration from cotyledon explants of bottle gourd (Lagneria siceraria Standl.) Plant Cell Reports, 23(5): 291-296.
    8. Narayan J P. (2016) Ex-situ Conservation of the rare and threatened medicinal climber Corallocarpus epigaeus Rottler through In vitro regeneration method. British Biotechnology Journal, 14(3): 1-10.
    9. Rahmatullah Md, Nur Kabidul Azam Md, Zubedah Khatun, Syeda Seraj, Farhana Islam, Atiqur Rahman Md, Sharmina Jahan, Shah Aziz Md. (2012) Medicinal plants used for the treatment of Diabetes by the Marakh Sect of the Garo tribe living in Mymensingh district, Bangladesh. African Network on Ethnomedicines, 9(3): 380-385.
    10. Ramasubbu R and Chandraprabha A. (2014) Micropropagation of Citrullus colocynthis (L.) Schrader: An important antidiabetic Plant. New Horizons in Biotechnology and Bio and medicines, 271-275. 
    11. Randhir Singh, Lalit Kumar, Navpreet Kaur, Samrat Chauhan. (2014) Phyto-Pharmacological Review of Coccinia indica. World Journal of Pharmacy and Pharmaceutical Sciences, 3(2): 1734-1745. 
    12. Sarkar P, FMS, Jahan R. and Rahmatullah M. (2008) In vitro regeneration of Coccinia grandis (L.) Voigt. An indigineous medicinal plant of Bangladesh. Afr. J. Trad. Comp. Alt. Med. 
    13. Senthil Kumar P, Paulsamy S, Vijayakumar K K and Kalimuthu K. (2007) In vitro regeneration of the medicinal herbs of Nilgiri Shola, Acmella calva L. from leaf derived callus. Plant Tissue Culture and Biotechnology, 17(2): 109-114. 
    14. Shekhawat M S, Ravindran C P and Manokari M. (2014) Developmental and Hormonal Regulation of direct shoots and roots regeneration in Coccinia indica. International Journal of Natural Sciences Research, 2(7): 103-112.
    15. Shekhawat M S, Shekhawat M S, Harish R, Kheta M, Phulwaria, and Gupta A K. (2011) High frequency plantlet regeneration from nodal segment culture of female Momordica dioica (Roxb.), Journal Crop Sci. Biotech,14: 133-137.
    16. Shyam Kumar B, Gnanasekaran D, Jaishree V and Channabasavaraj K P. (2010) Hepatoprotective activity of Coccinia indica leaves extract. International Journal of Pharmaceutical and Biomedical Research, 1(4): 154-156.
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