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Research Article

volume 52 issue 3 (june 2018) : 295-299,   Doi: 10.18805/IJARe.A-4913

Efficacy of non formulated entomopathogenic fungi (Beauveria bassiana) to control the storage insect pests of green gram (Vigna radiata) seeds 

R
Ranjana Pande
H
H.N. Mishra
1Department of Agricultural and Food Engineering, Indian Institute of Technology, Kharagpur-721 302, West Bengal, India.
Cite article:- Pande Ranjana, Mishra H.N. (NaN). Efficacy of non formulated entomopathogenic fungi (Beauveria bassiana) to control the storage insect pests of green gram (Vigna radiata) seeds. Indian Journal of Agricultural Research. 52(3): 295-299. doi: 10.18805/IJARe.A-4913.

ABSTRACT

The concepts and practical applications of fungal biotechnology continue to make significant contributions to food science. Storage of grains is part of the post-harvest system through which food material passes on its way from field to consumer. Stored pulses are severely infested by beetles of the family Bruchidae (Coleoptera). The present study was focused on the efficacy of microbial pesticides, i.e entomopathogenic fungus (Beauveria bassiana) on the Callosobruchus species were investigated in laboratory in stored green gram (Vigna radiata) seeds. Four different conidial concentrations of B. bassiana were sprayed (1 mL) based on the logarithmic series (4×102, 4×103, 4×104 and 4×105 conidia mL-1) and incubated for 10 days. Twenty four experiments were conducted with a complete randomized block design (CRD) with three replications. B.bassiana, concentration of, 4×105 conidia mL-1 at exposure day 10 gave maximum (99.00 %) insect mortality, moisture content of seed was 11.48% and protein content was 24.5%. Optimally fungal treated samples were devoid of any mycotoxins thereby implying their safety for human consumption. The results indicated that the fungal treatment retain the germination quality of seed. This study provided a novel and environmentally safe technique.

REFERENCES

  1. Adane, K., Moore, D., Archer, S.A. (1996). Preliminary studies on the use of Beauveria bassiana to control Sitophilus zeamais (Coleoptera: Curculionidae) in the laboratory. Journal of Stored Products Research, 32:105-113.
  2. Akhtari, N., Sardar, M.A., Rahman, M.H., Abedin, S.M.Z., Rahman, A.K.M.M. (1993). Feeding and oviposition preference of pulse beetle, C. chinensis on different genotypes of bean and its control. Annual Bangladesh Agriculture, 3(2):63-69.
  3. Ansari, M.S., Ahmad, N., Hasan, F. (2012). Environmental strategies for sustainable development. In: Malik, A. and Grohmann, E. (ed.), Strategies for Sustainability in Potential of Biopecticides in Sustainable Agriculture. Springer Science + Business Media, B.V, pp. 529–595.
  4. Bidochka, M.J., Clark, D.C., Lewis, M.W., Keyhani, N.O. (2010). Could insect phagocytic avoidance by entomogenous fungi have evolved via selection against soil amoeboid predators? Microbiology, 156: 2164-2171. 
  5. Birewar, B.R. (1984). Post-Harvest Technology of Pulses, Pulse Production - Constraints and Opportunities. Oxford and IBH Publishing Co., New Delhi, India pp. 425–438.
  6. Cherry, A.J., Abalo, P., Hello, K. (2005). A laboratory assessment of the potential of different strains of the entomopathogenic fungi Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metschnikoff) to control Callosobruchus maculatus F. (Coleoptera: Bruchidae) in stored cowpea. Journal of Stored Products Research, 41:295-309.
  7. Clemson., Extension (Clemson HGIC), Home and Garden Information Center (2007) Organic Pesticides and Biopesticides. URL: http://hgic.clemson.edu/factsheets/HGIC2756.htm. Accessed on 22 May 2012. Clemson (SC): Clemson University.
  8. Deshpande, S.D., Singh, G. (2011). “Long term storage structures in pulses”, National symposium on pulses for sustainable agriculture and nutritional security, Indian Institute of Pulses Research, April 2001, New Delhi, pp.17–1. 
  9. Donangelo, C.M., Trugo, L.C., Trugo, M.F., Eggum, B.O. (1995). Effect of germination of legume seeds on chemical composition and on protein and energy utilization in rats. Food Chemistry, 53: 23-27.
  10. Droby, S., Wisniewski, M., Macarisin, D., Wilson, C. (2009). Twenty years of postharvest biocontrol research: It is time for a new paradigm? Postharvest Biology and Technology, 52: 137-145.
  11. Mahdneshin, Z., Safaralizadeh, M.H., Ghosta, Y. (2009). Study on the efficacy of Iranian Isolates of Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metsch.) Sorokin against Rhyzoperta domonica F. (Coleoptera: Bostrichidae). Journal of Biological Science, 9:170-174.
  12. Quesada-Moraga E., Vey, A. (2004). Bassiacridin, a protein toxic for locust secreted by the entomopathogenic fungus Beauveria bassiana. Mycological Research, 108:441–452.
  13. Rangaswamy, R. (2000). Complete randomized design. A Textbook of Agricultural Statistics. New Age International (Pvt.) Ltd. Publishers, New Delhi, pp 269–314.
  14. Reddy, A.A. (2009). Pulse production technology: Status and way forward. Review Agriculture, 52: 73"80.
  15. Song, H.H., Lee, H.S., Jeong, J.H., Park, H.S., Lee, C. (2008) Diversity in Beauvericin and Enniatins HI, and MK 1688 by Fusarium oxysporum isolated from potato. International Journal of Food Microbiology, 122:296-201.
  16. Suchita, M.G., Reddy, G.P.U., Murthy, M.M.K. (1989). Relative efficacy of pyrethroids against Rice Weevil (Sitophilus oryzae L.) infesting stored wheat. Indian Journal of Plant Protection, 17:243-246.
  17. Sumit, K., Bourai, V.K., Hitendra, K. (2011). Post harvest losses in pulses of Uttarakhand: A specific study of sample villages of Assan valley. Economic Affairs, 56:243"247.
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    Research Article

    volume 52 issue 3 (june 2018) : 295-299,   Doi: 10.18805/IJARe.A-4913

    Efficacy of non formulated entomopathogenic fungi (Beauveria bassiana) to control the storage insect pests of green gram (Vigna radiata) seeds 

    R
    Ranjana Pande
    H
    H.N. Mishra
    1Department of Agricultural and Food Engineering, Indian Institute of Technology, Kharagpur-721 302, West Bengal, India.
    Cite article:- Pande Ranjana, Mishra H.N. (NaN). Efficacy of non formulated entomopathogenic fungi (Beauveria bassiana) to control the storage insect pests of green gram (Vigna radiata) seeds. Indian Journal of Agricultural Research. 52(3): 295-299. doi: 10.18805/IJARe.A-4913.

    ABSTRACT

    The concepts and practical applications of fungal biotechnology continue to make significant contributions to food science. Storage of grains is part of the post-harvest system through which food material passes on its way from field to consumer. Stored pulses are severely infested by beetles of the family Bruchidae (Coleoptera). The present study was focused on the efficacy of microbial pesticides, i.e entomopathogenic fungus (Beauveria bassiana) on the Callosobruchus species were investigated in laboratory in stored green gram (Vigna radiata) seeds. Four different conidial concentrations of B. bassiana were sprayed (1 mL) based on the logarithmic series (4×102, 4×103, 4×104 and 4×105 conidia mL-1) and incubated for 10 days. Twenty four experiments were conducted with a complete randomized block design (CRD) with three replications. B.bassiana, concentration of, 4×105 conidia mL-1 at exposure day 10 gave maximum (99.00 %) insect mortality, moisture content of seed was 11.48% and protein content was 24.5%. Optimally fungal treated samples were devoid of any mycotoxins thereby implying their safety for human consumption. The results indicated that the fungal treatment retain the germination quality of seed. This study provided a novel and environmentally safe technique.

    REFERENCES

    1. Adane, K., Moore, D., Archer, S.A. (1996). Preliminary studies on the use of Beauveria bassiana to control Sitophilus zeamais (Coleoptera: Curculionidae) in the laboratory. Journal of Stored Products Research, 32:105-113.
    2. Akhtari, N., Sardar, M.A., Rahman, M.H., Abedin, S.M.Z., Rahman, A.K.M.M. (1993). Feeding and oviposition preference of pulse beetle, C. chinensis on different genotypes of bean and its control. Annual Bangladesh Agriculture, 3(2):63-69.
    3. Ansari, M.S., Ahmad, N., Hasan, F. (2012). Environmental strategies for sustainable development. In: Malik, A. and Grohmann, E. (ed.), Strategies for Sustainability in Potential of Biopecticides in Sustainable Agriculture. Springer Science + Business Media, B.V, pp. 529–595.
    4. Bidochka, M.J., Clark, D.C., Lewis, M.W., Keyhani, N.O. (2010). Could insect phagocytic avoidance by entomogenous fungi have evolved via selection against soil amoeboid predators? Microbiology, 156: 2164-2171. 
    5. Birewar, B.R. (1984). Post-Harvest Technology of Pulses, Pulse Production - Constraints and Opportunities. Oxford and IBH Publishing Co., New Delhi, India pp. 425–438.
    6. Cherry, A.J., Abalo, P., Hello, K. (2005). A laboratory assessment of the potential of different strains of the entomopathogenic fungi Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metschnikoff) to control Callosobruchus maculatus F. (Coleoptera: Bruchidae) in stored cowpea. Journal of Stored Products Research, 41:295-309.
    7. Clemson., Extension (Clemson HGIC), Home and Garden Information Center (2007) Organic Pesticides and Biopesticides. URL: http://hgic.clemson.edu/factsheets/HGIC2756.htm. Accessed on 22 May 2012. Clemson (SC): Clemson University.
    8. Deshpande, S.D., Singh, G. (2011). “Long term storage structures in pulses”, National symposium on pulses for sustainable agriculture and nutritional security, Indian Institute of Pulses Research, April 2001, New Delhi, pp.17–1. 
    9. Donangelo, C.M., Trugo, L.C., Trugo, M.F., Eggum, B.O. (1995). Effect of germination of legume seeds on chemical composition and on protein and energy utilization in rats. Food Chemistry, 53: 23-27.
    10. Droby, S., Wisniewski, M., Macarisin, D., Wilson, C. (2009). Twenty years of postharvest biocontrol research: It is time for a new paradigm? Postharvest Biology and Technology, 52: 137-145.
    11. Mahdneshin, Z., Safaralizadeh, M.H., Ghosta, Y. (2009). Study on the efficacy of Iranian Isolates of Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metsch.) Sorokin against Rhyzoperta domonica F. (Coleoptera: Bostrichidae). Journal of Biological Science, 9:170-174.
    12. Quesada-Moraga E., Vey, A. (2004). Bassiacridin, a protein toxic for locust secreted by the entomopathogenic fungus Beauveria bassiana. Mycological Research, 108:441–452.
    13. Rangaswamy, R. (2000). Complete randomized design. A Textbook of Agricultural Statistics. New Age International (Pvt.) Ltd. Publishers, New Delhi, pp 269–314.
    14. Reddy, A.A. (2009). Pulse production technology: Status and way forward. Review Agriculture, 52: 73"80.
    15. Song, H.H., Lee, H.S., Jeong, J.H., Park, H.S., Lee, C. (2008) Diversity in Beauvericin and Enniatins HI, and MK 1688 by Fusarium oxysporum isolated from potato. International Journal of Food Microbiology, 122:296-201.
    16. Suchita, M.G., Reddy, G.P.U., Murthy, M.M.K. (1989). Relative efficacy of pyrethroids against Rice Weevil (Sitophilus oryzae L.) infesting stored wheat. Indian Journal of Plant Protection, 17:243-246.
    17. Sumit, K., Bourai, V.K., Hitendra, K. (2011). Post harvest losses in pulses of Uttarakhand: A specific study of sample villages of Assan valley. Economic Affairs, 56:243"247.
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