Indian Journal of Agricultural Research

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Research Article

Indian Journal of Agricultural Research, volume 52 issue 1 (february 2018) : 66-70

Three step protocol for regeneration of plantlets in Indian garlic varieties using root meristem

Ashwini P. Benke, Punam Shelke, V. Mahajan
1ICAR-Directorate of Onion and Garlic Research, Rajgurunagar-410 505, Pune, Maharashtra, India
Cite article:- Benke P. Ashwini, Shelke Punam, Mahajan V. (2018). Three step protocol for regeneration of plantlets in Indian garlic varieties using root meristem. Indian Journal of Agricultural Research. 52(1): 66-70. doi: 10.18805/IJARe.A-4874.

ABSTRACT

Protocol for plantlets regeneration through callus induction using root meristem was developed in present investigation. Different mediums were standardized for each step vis-a-via callus induction, callus proliferation and shoot regeneration. In first stage, B5 medium added with 0.7mg/l 2, 4-D and 1mg/l BAP showed 100% callusing while reduction in both growth regulators up to level of 0.5 mg/l 2,4-D and 0.1mg/l BAP results in significant callus proliferation. In last step, shoot regeneration was found to good and healthier in B5 medium supplemented with 0.1 mg/l 2, 4-D only. After regeneration normal growth in plantlets observed under plain B-5 medium, however no abnormality has been noted in developed population. Present protocol will be efficiently useful for chemical mutagen induction studies through callus, observing somaclonal variations and transgenic studies.

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