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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 51 issue 6 (december 2017) : 543-549,   Doi: 10.18805/IJARe.A-4795

Analysis of genetic variations in Musa paradisiaca cv Karibale-Monthan using AFLP markers

R
R. Shashikumar
V
V. Krishna
V
Venkatesh
S
S. Lokesha
1Department of Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta-577 451, Karnataka, India
Cite article:- Shashikumar R., Krishna V., Venkatesh, Lokesha S. (2017). Analysis of genetic variations in Musa paradisiaca cv Karibale-Monthanusing AFLP markers. Indian Journal of Agricultural Research. 51(6): 543-549. doi: 10.18805/IJARe.A-4795.

ABSTRACT

In the present study, the genetic variations of leaf calli regenerants of Musa paradisiaca cv Karibale-Monthan has been analyzed using AFLP markers. The highest (86.66%) frequency of compact callus formation was obtained when leaf explants were cultured on MS media, containing 3mg/L 2,4-D and 0.5 mg/L BAP. The optimum shoot formation and multiplication (5.70±1.49) was achieved from the leaf derived callus on MS medium fortified with 3mg/L BAP+0.5mg/L TDZ. The 3-4 cm small shoots produced distinct roots on MS media containing 0.5mg/l NAA with 0.2% activated charcoal. The AFLP analyses produced 1012 monomorphic bands from total 1094 bands with 7.5% polymorphism between mother plant and callus derived regenerants. The percentage of monomorphism for individual primer combinations was very high and varied from 90.3% (EcoRI-AG×MseI-CCG) to 97.6% (EcoRI-AT×MseI-CGA). The result showed lack of polymorphism is due to genetic integrity of the plants that was not altered indifferent to the hormonal treatment.

REFERENCES

  1. Agarwal, M., Shrivastava, N.,and Padh, H. (2008). Advances in molecular marker techniques and their applications in plant science. Plant Cell Report, 27:617-631.
  2. Alexandra, P.D., Monica, G.M., Ronald, E.W., Beatriz, M.M. (2001). Anthocyanins from banana bracts (Musa X paradisiaca) as potential food colorants. Food Chemistry, 73(3):327-32.
  3. Badgujar, C.D., Deshmukh, S.S., Dusane, S.M. (2005). A field comparison of conventional suckers with in-vitro derived planting material of Basrai banana. Agricultural Science Digest, 25(2):149 -150.
  4. Bairu, M.W., Fennell, C.W., van Staden, J. (2006). The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. ‘Zelig’). Scientia Horticulturae, 108:347-351.
  5. Cote, F.X., Sandoval, J.A., Marie Ph., Auboiron, E. (1993). Variations in micropropagated bananas and plantains: literature survey. Fruits, 48:15-22.
  6. Crouch, J.H., Crouch, H.K., Constandt, H., Van Gysel, A., Breyne, P., van Montagu, M., Jarret, R.L., Ortiz, R. (1999). Comparison of PCR-based molecular marker analyses of Musa breeding populations. Molecular Breeding, 5:233-244.
  7. Damasco, O.P., Smith, M.K., Adkins, S.W., Godwin, I.D. (1998). Use of SCARbased marker for early detection of dwarf off-types in micropropagated ‘Cavendish’ bananas. Acta Horticulturae, 461:157-164.
  8. Doyle, J.J., and Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19:11-15.
  9. Gort, G., Koopman, W.J.M., Stein, A. (2006). Fragment length distributions and collision probabilities for AFLP markers. Biometrics, 62:1107-1115.
  10. Hakim, L., Ahmed, G., Kabir, H., Mamun, A.N.K., Roy, P.K., Islam, M.R., Khan, R. (2008). Production of doubled haploids in banana. FAO/IAEA International Symposium Proceedings, 121.
  11. Hwang, S.C. and Tang, C.Y. (1996). Somaclonal variation and its use for improving Cavendish (AAA dessert) bananas in Taiwan. Musarama, 9(3):6.
  12. Jagadishchandra, K.S. and Sathyanarayana, N. (2001). Regeneration of plants in mulberry (Morus indica) var. Mysore local through leaf culture. J. Plant Biol.28:147 – 152.
  13. Karp, A. (1994). Origins, causes and uses of variation in plant tissue cultures. Kluwer Academic Publishers Dordrecht. 139-152.
  14. Khadiga, G.A.E., Rasheid, S.M., Khalafalla, M.M. (2009). Effect of plant growth regulators on callus induction and plant regeneration in tuber segment culture of potato (Solanum tuberosum L.) cultivar Diamant. African. Journal Biotechnology, 8:2529-2534.
  15. Khanam, D., Hoque, M.A., Khan, M.A., Quasem, A. (1996). In vitro propagation of banana (Musa spp). Plant Tissue Culture, 6(2):89-94.
  16. Kour, G., Bakshi, P., Wali, V.K., Jastotia, A. (2011). Role of molecular markers in some perennial crops- Brief Review. Agriculture Reviews, 32(4):256-267.
  17. Kulkarni, V.M., Suprasanna, P.,and Bapat, V.A. (2006). Plant regeneration through multiple shoot formation and somatic embryogenesis in a commercially important and endangered Indian banana cv. Rajeli. Current Science, 90(6):842-846.
  18. Oh, T.J., Cullis, M.A., Kunert, K., Engelborghs, I., Swennen, R.,and Cullis, C.A. (2007). Genomic changes associated with somaclonal variation in banana (Musa spp.). Physiology of Plant, 129:766-774.
  19. Reuveni, O., Israeli, Y.,and Golubowicz, S. (1993). Factors influencing the occurrence of somaclonal variation in micropropagated bananas. Acta Horticulturae, 336:357-364.
  20. Sampath Kumar, K.P., Bowmic, D., Duraivel, S.,and Umadevi, M. (2012). Traditional and medicinal uses of banana. Journal of Pharmacognosy and Phytochemistry, 1(3):51-63.
  21. Shashikumar, R., Krishna V.,and Venkatesh (2015). High frequency plant regeneration of Musa paradisiaca cv. Karibale Monthan. International Journal Applied Sciences and Biotechnology, 3(2): 202-209.
  22. Smith, M.K. (1988). A review of factors influencing the genetic stability of micropropagated bananas. Fruits, 43:219-223.
  23. Sridhar, T.M., and Naidu, C.V. (2011). An efficient callus induction and plant regeneration of Solanum nigrum (L.) - An important antiulcer medicinal plant. Journal of Phytology, 3(5):23-28.
  24. Stover, R.H. (1987). Somaclonal variation in Grande Naine and Saba bananas in the nursery and in the field. ACIAR Proceeding, Canberra. 
  25. Sunpui, W. and Kanchanapoom, K. (2002). Plant regeneration from petiole and leaf of African violet (Saintpaulia ionantha) cultured In vitro. Songklanakarin. Journal of Science and Technology, 24(3):257-264.
  26. Tang, C.Y., Liu, C.C.,and Hwang, S.C. (2000). Improvement of the horticultural traits of Cavendish banana (Musa spp., AAA group I). Selection and evaluation of a semi-dwarf clone resistant to Fusarium wilt. Journal of Chinese Society for Horticultural Science, 46:173-182
  27. Tripathy, S.K., Panda, A. Nayak, P.K., Dash, S., Lenka, D., Mishra, D.R., Kar, R.K., Senapati, N.,and Dash, G.B. (2016). Somaclonal variation for genetic improvement in grasspea (Lathyrus sativus L.). Legume Research, 39(3):329-335.
  28. Wong, C., Kiew, R.,and Loh, J.P. (2001). Genetic diversity of the wild bananan Musa acuminata Colla in Malaysia as evidenced by AFLP. Annual Botany, 88(6):1017-1025.
  29. Zaffari, G.R., Peres, L.E.P., Kerbauy, G.B. (1998). Endogenous levels of cytokinins, indoleacetic acid, abscisic acid, and pigments in variegated somaclones of micropropagated banana leaves. Journal of Plant Growth Regulators, 17:59-61.
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Indian Journal of Agricultural Research
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    Research Article

    volume 51 issue 6 (december 2017) : 543-549,   Doi: 10.18805/IJARe.A-4795

    Analysis of genetic variations in Musa paradisiaca cv Karibale-Monthan using AFLP markers

    R
    R. Shashikumar
    V
    V. Krishna
    V
    Venkatesh
    S
    S. Lokesha
    1Department of Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta-577 451, Karnataka, India
    Cite article:- Shashikumar R., Krishna V., Venkatesh, Lokesha S. (2017). Analysis of genetic variations in Musa paradisiaca cv Karibale-Monthanusing AFLP markers. Indian Journal of Agricultural Research. 51(6): 543-549. doi: 10.18805/IJARe.A-4795.

    ABSTRACT

    In the present study, the genetic variations of leaf calli regenerants of Musa paradisiaca cv Karibale-Monthan has been analyzed using AFLP markers. The highest (86.66%) frequency of compact callus formation was obtained when leaf explants were cultured on MS media, containing 3mg/L 2,4-D and 0.5 mg/L BAP. The optimum shoot formation and multiplication (5.70±1.49) was achieved from the leaf derived callus on MS medium fortified with 3mg/L BAP+0.5mg/L TDZ. The 3-4 cm small shoots produced distinct roots on MS media containing 0.5mg/l NAA with 0.2% activated charcoal. The AFLP analyses produced 1012 monomorphic bands from total 1094 bands with 7.5% polymorphism between mother plant and callus derived regenerants. The percentage of monomorphism for individual primer combinations was very high and varied from 90.3% (EcoRI-AG×MseI-CCG) to 97.6% (EcoRI-AT×MseI-CGA). The result showed lack of polymorphism is due to genetic integrity of the plants that was not altered indifferent to the hormonal treatment.

    REFERENCES

    1. Agarwal, M., Shrivastava, N.,and Padh, H. (2008). Advances in molecular marker techniques and their applications in plant science. Plant Cell Report, 27:617-631.
    2. Alexandra, P.D., Monica, G.M., Ronald, E.W., Beatriz, M.M. (2001). Anthocyanins from banana bracts (Musa X paradisiaca) as potential food colorants. Food Chemistry, 73(3):327-32.
    3. Badgujar, C.D., Deshmukh, S.S., Dusane, S.M. (2005). A field comparison of conventional suckers with in-vitro derived planting material of Basrai banana. Agricultural Science Digest, 25(2):149 -150.
    4. Bairu, M.W., Fennell, C.W., van Staden, J. (2006). The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. ‘Zelig’). Scientia Horticulturae, 108:347-351.
    5. Cote, F.X., Sandoval, J.A., Marie Ph., Auboiron, E. (1993). Variations in micropropagated bananas and plantains: literature survey. Fruits, 48:15-22.
    6. Crouch, J.H., Crouch, H.K., Constandt, H., Van Gysel, A., Breyne, P., van Montagu, M., Jarret, R.L., Ortiz, R. (1999). Comparison of PCR-based molecular marker analyses of Musa breeding populations. Molecular Breeding, 5:233-244.
    7. Damasco, O.P., Smith, M.K., Adkins, S.W., Godwin, I.D. (1998). Use of SCARbased marker for early detection of dwarf off-types in micropropagated ‘Cavendish’ bananas. Acta Horticulturae, 461:157-164.
    8. Doyle, J.J., and Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19:11-15.
    9. Gort, G., Koopman, W.J.M., Stein, A. (2006). Fragment length distributions and collision probabilities for AFLP markers. Biometrics, 62:1107-1115.
    10. Hakim, L., Ahmed, G., Kabir, H., Mamun, A.N.K., Roy, P.K., Islam, M.R., Khan, R. (2008). Production of doubled haploids in banana. FAO/IAEA International Symposium Proceedings, 121.
    11. Hwang, S.C. and Tang, C.Y. (1996). Somaclonal variation and its use for improving Cavendish (AAA dessert) bananas in Taiwan. Musarama, 9(3):6.
    12. Jagadishchandra, K.S. and Sathyanarayana, N. (2001). Regeneration of plants in mulberry (Morus indica) var. Mysore local through leaf culture. J. Plant Biol.28:147 – 152.
    13. Karp, A. (1994). Origins, causes and uses of variation in plant tissue cultures. Kluwer Academic Publishers Dordrecht. 139-152.
    14. Khadiga, G.A.E., Rasheid, S.M., Khalafalla, M.M. (2009). Effect of plant growth regulators on callus induction and plant regeneration in tuber segment culture of potato (Solanum tuberosum L.) cultivar Diamant. African. Journal Biotechnology, 8:2529-2534.
    15. Khanam, D., Hoque, M.A., Khan, M.A., Quasem, A. (1996). In vitro propagation of banana (Musa spp). Plant Tissue Culture, 6(2):89-94.
    16. Kour, G., Bakshi, P., Wali, V.K., Jastotia, A. (2011). Role of molecular markers in some perennial crops- Brief Review. Agriculture Reviews, 32(4):256-267.
    17. Kulkarni, V.M., Suprasanna, P.,and Bapat, V.A. (2006). Plant regeneration through multiple shoot formation and somatic embryogenesis in a commercially important and endangered Indian banana cv. Rajeli. Current Science, 90(6):842-846.
    18. Oh, T.J., Cullis, M.A., Kunert, K., Engelborghs, I., Swennen, R.,and Cullis, C.A. (2007). Genomic changes associated with somaclonal variation in banana (Musa spp.). Physiology of Plant, 129:766-774.
    19. Reuveni, O., Israeli, Y.,and Golubowicz, S. (1993). Factors influencing the occurrence of somaclonal variation in micropropagated bananas. Acta Horticulturae, 336:357-364.
    20. Sampath Kumar, K.P., Bowmic, D., Duraivel, S.,and Umadevi, M. (2012). Traditional and medicinal uses of banana. Journal of Pharmacognosy and Phytochemistry, 1(3):51-63.
    21. Shashikumar, R., Krishna V.,and Venkatesh (2015). High frequency plant regeneration of Musa paradisiaca cv. Karibale Monthan. International Journal Applied Sciences and Biotechnology, 3(2): 202-209.
    22. Smith, M.K. (1988). A review of factors influencing the genetic stability of micropropagated bananas. Fruits, 43:219-223.
    23. Sridhar, T.M., and Naidu, C.V. (2011). An efficient callus induction and plant regeneration of Solanum nigrum (L.) - An important antiulcer medicinal plant. Journal of Phytology, 3(5):23-28.
    24. Stover, R.H. (1987). Somaclonal variation in Grande Naine and Saba bananas in the nursery and in the field. ACIAR Proceeding, Canberra. 
    25. Sunpui, W. and Kanchanapoom, K. (2002). Plant regeneration from petiole and leaf of African violet (Saintpaulia ionantha) cultured In vitro. Songklanakarin. Journal of Science and Technology, 24(3):257-264.
    26. Tang, C.Y., Liu, C.C.,and Hwang, S.C. (2000). Improvement of the horticultural traits of Cavendish banana (Musa spp., AAA group I). Selection and evaluation of a semi-dwarf clone resistant to Fusarium wilt. Journal of Chinese Society for Horticultural Science, 46:173-182
    27. Tripathy, S.K., Panda, A. Nayak, P.K., Dash, S., Lenka, D., Mishra, D.R., Kar, R.K., Senapati, N.,and Dash, G.B. (2016). Somaclonal variation for genetic improvement in grasspea (Lathyrus sativus L.). Legume Research, 39(3):329-335.
    28. Wong, C., Kiew, R.,and Loh, J.P. (2001). Genetic diversity of the wild bananan Musa acuminata Colla in Malaysia as evidenced by AFLP. Annual Botany, 88(6):1017-1025.
    29. Zaffari, G.R., Peres, L.E.P., Kerbauy, G.B. (1998). Endogenous levels of cytokinins, indoleacetic acid, abscisic acid, and pigments in variegated somaclones of micropropagated banana leaves. Journal of Plant Growth Regulators, 17:59-61.
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