Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

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Indian Journal of Agricultural Research, volume 51 issue 3 (june 2017) : 221-226

Synergetic effect of different free-living diazotrophic bacteria, P – solubilizing bacteria and Rhizobium on growth of Oryza sativa L. (cv. NDR – 359)

Mihirlal Roy*, Sibani Saha, Jhuma Das
1<p>Tripura State Council for Science &amp; Technology,&nbsp;A.R.Complex, Agartala, Tripura - 799 006 India.</p>
Cite article:- Roy* Mihirlal, Saha Sibani, Das Jhuma (2017). Synergetic effect of different free-living diazotrophic bacteria, P &ndash; solubilizing bacteria and Rhizobium on growth of Oryza sativa L. (cv. NDR &ndash; 359) . Indian Journal of Agricultural Research. 51(3): 221-226. doi: 10.18805/ijare.v51i03.7910.

The study was conducted to observe the individual and synergetic effect of certain free-living diazotrophic bacteria, viz., Azospirillum brasilense, Azotobacter chroococcum, Clostridium pasteurianum; a P-solubilising bacteria, viz., Pseudomonous denitrificans and Rhizobium on growth of rice cultivar NDR – 359. The resuts showed that all the inoculated strains both individually and in combinations played a positive role in enhancement of growth of rice cultivar. It was also observed that multi-strain inoculation responded better, which indicated their synergetic effect.

  1. Askary, M., Mostaijeron, A., Amoaghaei, R. and Mostaijeron, A. (2009). Influence of the co-inoculation of Azospirillum brasilense and Rhizobium meliloti plus 2, 4 – D on grain yield and N, P, K content of Triticum aestivum (cv. Baccros and Mahadavi). American-Eurasian J. Agric. Environ. Sci., 5(3) : 296 – 307.

  2. Baldani, V.L.D., Baldani, J.I and Dobereiner, J. (1987). Inoculation of field-grown wheat (Triticum aestivum) with Azospirillum spp. in Brazil. Biol. Fertil. Soils, 457 – 60.

  3. Baldani, V.L.D; Baldani, J.I and Dobereiner, J. (1983). Effects of Azospirillum inoculation on root infection and nitrogen incorporation in wheat. Can. J. Microbiol. 29 : 924 – 929.

  4. Bashan, Y. and Holguin, G. (1997). Azospirillum – plant relationships : environmental and physiological advances (1990 – 1996). Can. J. Microbiol., 43 : 103 – 121.

  5. Bashan, Y; Holguin, G and De-Beshan, L.E. (2004). Azospirillum – plant relationship : physiological, molecular, agriculture and environmental advances (1997 – 2003). Can. J. Microbiol, 50 : 521 – 577.

  6. Biro, B., Koves-Pechy, K., Vores, I., Eggenberger, P. and Strasser, R. J. (2000). Interrelations between Azospirillum and Rhizobium nitrogen-fixers and arbuscular mycorrhizal fungi in the rhizosphere of alfalfa in sterile, AMF – free or normal conditions. Appl. Soil Eco., 15 : 159 –168.

  7. Biswas, J.C., Ladha, L.K and Dazzo, F.B (2000a). Rhizobia inoculation improves nutrient uptake and growth of low land rice. Soil. Sci Soc. Amm.J. 64 : 1644 – 1650.

  8. Biswas, J.C; Ladha, J.K and Dazzo, F.B (2000b). Rhizobial inoculation influences seedling vigour and yield of rice. Agronomy Journal. 92 : 880 – 886.

  9. Boddey, R.M; Baldani, V.L.D; Baldani, J.I and Dobereiner, J. (1986). Effect of inoculation of Azospirillum sp. on nitrogen fixation by field grown wheat. Plant Soil. 95 : 109 – 121.

  10. Boddey, R.M; Urquiaga, S; Reis, V.M and Dobereiner, J. (1991). Biological nitrogen fixation associated with sugarcane. Plant Soil. 137 : 111 – 117.

  11. Camacho, L. R., Constant, P., Raynaud, C., Laneelle, M. A., Triccas, J. A., Gicquel, B., Daffe, M. & Guilhot, C. (2001). Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis: Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem., 276 : 19845-19854.

  12. Choudhury, A.T.M.A and Kennedy IR (2005). Nitrogen fertilizer losses from rice soils and control of environmental pollution problems. Soil Sci and Plant Analysis 36 : 1625 – 1639.

  13. Choudhury, A.T.M.A. and Khanif, Y.M.(2004). Effects of nitrogen and copper fertilization on rice yield and fertilizer nitrogen efficiency : A 15N tracer study. Pak J. of Scient. and Indust. Res., 47: 50 – 55.

  14. Chowdhury, A.T.M.A and Khanif, Y.M (2001). Evaluation of the effects of nitrogen and magnesium fertiliz on rice yield and fertilizer nitrogen efficiency using 15N tracer technique. J of Plant Nutr 24 : 855 – 871.

  15. Chowdhury, A.T.M.A and Khanif, Y.M. (2004). Effects of nitrogen and copper fertilization on rice yield and fertilizer nitrogen efficiency : A 15N tracer study. Pak J of Scient and Indust Res 47 : 50 – 55 .

  16. Das, A.C and Saha, D. (2003). Influence of diazotrophic inoculations on nitrogen nutrition of rice. Aust. J. Soil Res. 41 (8) : 1543 – 1554.

  17. De Datta, S.K. (1978). Fertilizer management for efficient use in wetland rice soils. In : Ponnamperuma, F.N., (ed). Soils and rice. Los Bans, the Philippines : International Rice Research Institute. pp 671 – 701.

  18. De Datta, S.K. and Buresh, R.J. (1989). Integrated nitrogen management in irrigated rice. Advances in Soil Scie. 10 : 143 – 169.

  19. Dey, B and Srivastava, R.C (2001). Biodiversity of Rhizobium species in respect of theior growth and nitrogen-fixing efficiency in free-living culture. Vegetos, 14 : 45 – 49.

  20. Fakir, M.A.I; Hassan, S.M.R and Sattar, M.A (2008) Growth and yield of rice as influenced by Azotobacter and Azospirillum inoculation in presence and absence of Urea – N. J. of the Bangladesh Soc for Agri Sci and Tech. 4: 247 – 250.

  21. Garbet, S; Ryan, J and Wood, M (1998). Nitrogen and Water effects on wheat yield in a Mediterranean – type climate. II. Fertiliser-use efficiency with labeled nitrogen. Field Crop Research. 58 : 213 – 221.

  22. Glick, B.R. (1995). The enhancement of plant growth by free-living bacteria. Can. J. Microbiol., 41 : 109 – 117.

  23. Hadi, F. and Bano, A. (2010). Effect of diazotrophs (Rhizobium and Azotobacter) on growth of maize (Zea mays L.) and accumulation of lead (pb) in different plant parts. Polish.J. Bot., 42(6) : 4363 – 4370.

  24. Halder, A.K., Misra, A. K. and Chakraborty, P.K. (1991). Solubilisation of inorganic phosphates by Bradyrhizobium. Indian J. Exp. Biol., 29 : 28 – 31.

  25. Halvorson, A.D; Follett, R.F; Bartolo, M.E and Schweissing, F.C. (2002). Nitrogen fertilizer use efficiency of furrow-irrigated onion and corn. Agron J. 94 : 442 – 449.

  26. Hegazi, N.A., Fayez, M., Amin, G., Hamza, M.A., Abbas, M., Youssef, H. and Monib, M. (1998). Diazotrophs associated with non-    legumes grown in sandy soils. In : Nitrogen Fixation with Non-legumes (Eds., Malik, K.A., Mirza, M.S. and Ladha, J.K.), Kluwer Academic Publishers, Dordrecht, pp. 209 – 222.

  27. Kennedy, I.R; Chowdhury, A.T.M.A and Kecskes, M.L (2004). Non-symbiotic bacterial diazotrophs in crop farming systems : can their potential for plant growth promotion be better exploited ? Soil. Biol. Biochem. 36 : 1229 – 1244.

  28. Kifle, M.H. and Laing, M.D. (2011). Determination of optimum dose and frequency of application of free-living diazotrophs (FLD) on lettuce. African J. Agri Res. 6(3) : 671 – 675.

  29. Kloepper, J.W., Leong, J., Teintze, M., and Schroth, M.N. (1980). Enhanced plant growth by siderophores produced by plant growth-    promoting rhizobacteria. Nature, 286 : 885 – 886.

  30. Malik, K.A., Mirza, M.S., Hassan, U., Mechnaz, S., Rasul, G., Haurat, J., Balley, R. and Normand, P. (2002). The role of plant-    associated beneficial bacteria in rice-wheat cropping system. In : Biofertilisers in Action (Eds., Kennedy, I.R. and Chowdhury, A.T.M.A.), Rural Industries Research and Development Corporation, Canberra, pp. 73 – 83.

  31. Mirza, M.S., Mehnaz, S., Normand, P., Prigent-Combaret, C., Moenne-Loccoz, Y., Bally, R. and Malik, K.A. (2006). Molecular characterization and PCR detection of a nitrogen-fixing Pseudomonas strain promoting rice growth. Biol. Fertil. Soils., 43 : 163 – 170.

  32. Mishustin, E.N. (1970). The importance of non-symbiotic nitrogen-fixing microorganisms in agriculture. Plant Soil. 32 : 545 – 554.

  33. Nguyen, (2003). The positive yield response of field-grown rice to inoculation with a multi-strain biofertiliser in the Hanoiarea, Vietnam. Symbiosis. 34 : 1 – 15.

  34. Nguyen, T.H; Kennedy, I.R and Roughley, R.J. (2002). The response of field grown rice to inoculation with a multistrain biofertilizer in the Hanoi district, Vietnam. In : Kennedy, I.R and Chowdhury, A.T.M.A. (Eds). Biofertilizers in Action. Rural Industries Research and Development Corporation, Canberra. pp. 37 – 44.

  35. Qureshi, M.A., Shakir, M.A., Iqbal, A., Akhtar, N. And Khan, A. (2011). Co-inoculation of phosphate solubilizing bacteria and rhizobia for improving growth and yield of Mingbean (Vigna radiata L.). J. Animul and Plant Sciences, 21 (3) : 491 – 497.

  36. Rajaee, S; Alikhani, H.A and Raiesi, F. (2007). Effect of Plant Growth Promoting Potentials of Azotobacter chroococcum native strains on growth, yield and uptake of nutrients in wheat. J.Sci. & Technol. Agric. & Nature. Resour., Vol.II.NO.41 (b), 297 – 301.

  37. Roy, M.L and Srivastava, R.C (2010). Single and Co-inoculation effects of different biofertilizers on growth, in vivo nitrate reductase activity and soluble protein in Oryza sativa L. J. Appl. Biosci. 36 (1) : 101 – 104.

  38. Russell, A.D., W.B. Hugo and Ayliffo, A.L. (1982). Principles and practices of disinfection, preservation and sterilization. Black Wall Scientific, London. pp.653 

  39. Sattar, M.A; Rahman, M.F; Das, D.K and Choudhury, T.M.A.A. (2008). Prospects of using Azotobacter, Azospirillum and cyanobacteria as supplements of urea nitrogen for rice production in Bangladesh. In : Proceedings of ACIR, NO – 130. pp. 59 – 66.

  40. Shaheen, A.M.; Fatma, A. Rizk; Omina, M. Sawan and A.A Ghoname. (2007). The Integrated use of bioinoculants and chemical nitrogen fertilizer on growth, yield and nutritive value of two Okra (Abelmoschus esculentus L) cultivars. Aust.J. of Basic and Applied Sciences, 1(3) : 307 – 312.

  41. Shrestha, R.K and Ladha, J.K. (1998). Nitrate in ground water and integration of nitrogen-catch crop in rice-sweet pepper cropping system. Soil. Sci. Soc. of America J. 62 : 1610 – 1619.

  42. Shrestha, R.K. and Maskey, S.L (2005). Associative nitrogen fixation in lowland rice. Nepal. Agric. Res. J. 6 : 112 – 121.

  43. Singh, B; Singh, Y and Sekhan, G.S. (1995). Fertiliser N use efficiency and nitrate pollution of ground water in developing countries. J. Contam. Hydrol. 20 : 167 – 184.

  44. Urquiaga, S; Cruz, K.H.S and Boddey, R.M. (1992). Contribution of nitrogen fixation to sugarcane : nitrogen – 15 and nitrogen-    balance estimates. Soil Sci. Soc. Am. J. 56 : 105 – 114.

  45. Vincent, J.M (1970). A manual for the practical study of root-nodule bacteria. IBP Hand Book No.15. International Biological Programme (IBP), 7, Marylebone Road, London, Blackwell Scientific Publication. Oxford. pp. 164. 

  46. Wairiu, M and Lal, R. (2003). Soil organic carbon in relation to cultivation and top soil removal on sloping lands of Kolombangara, Solon Islands. Soil and Tillage Res., 70 : 19 – 27.

  47. Williams, R.L. and Kennedy, I.R. (2002). A model for testing the effectiveness of biofertiliser for Australian rice production. In : Biofertilisers in Action (Eds., Kennedy, I.R. and Chowdhury, A.T.A.A.), Rural Industries Research and Development Corporation, Canberra, pp. 112 – 114.

  48. Wu, S.C; Cao, Z.H; Li, Z.G; Cheung, K.C and Wong, M.H. (2005). Effects of biofertiliser containing N-fixer, P and K solubilizers and AM fungi on maize growth : A green-house trial. Geoderma, 125 : 155 – 166.

  49. Yanni, Y. G., Rizk, R. Y., Abd El-Fattah, F. K., Squartini, A., Corich, V., Giacomni, A., de Bruijn, F., Rademaker, J., Maya-Flores, J., Ostrom, P., Vega-Hernandez, M., Hollingsworth, R. I., Martinez-Molina, E., Triplett, E., Umali-Garcia, M., Anarna, J.A., Rolfe, B.G., Ladha, J. K., Hill, J., Mujoo, R., Ng, P. K. and Dazzo, F. B. (2001). The beneficial plant growth-promoting association of Rhizobium leguminosarum bv. trifoli with rice roots. Aust. J. Plant Physol., 28 : 845-870.

  50. Yanni, Y. G., Rizk, R. Y., Maya-Flores, J. and Dazzo, F. B. (2001). Potential of the rice root occupant Rhizobium leguminosarum bv. Trifolii as plant growth-promoting biofertilizer for rice. In : 17th North American Conference on Symbiotic Nitrogen Fixtion. Program and Abstracts. University Laval, Quebec, pp. 79

  51. Yanni, Y., Rizk, R.Y., Corich, V., Squartini, A., Ninke, K., Philip – Hollingsworth, S., Orgambide, G., de Bruijn, F., Stoltzfus, J., Buckley, D., Schmidt, T.M., Matcos, P.F., Ladha, J.K. and Dazzo, F. B. (1997). Natural endophytic association between Rhizobium leguminosrrum bv. trifolii and rice roots and assessment of its potential to promote rice growth. Plant Soil, 194 : 99 – 114.

  52. Yanni, Y.G and Dazzo, F.B. (2010). Enhancement of rice production using endophytic strains of Rhizobium leguminosarum bv. trifolii in extensive field inoculation trials within Egypt Nile delta. Plant Soil, 336 : 129 – 142.


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