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Research Article

volume 54 issue 5 (october 2020) : 592-598,   Doi: 10.18805/IJARe.A-441

Effect of Hormonal Seed Priming on Germination, Growth, Yield and Biomass Allocation in Soybean Grown under Induced Drought Stress

P
P. Mangena
1Department of Biodiversity, School of Molecular and Life Sciences, Faculty of Science and Agriculture, University of Limpopo, Private Bag X1106, Sovenga 0727, South Africa.
Cite article:- Mangena P. (2020). Effect of Hormonal Seed Priming on Germination, Growth, Yield and Biomass Allocation in Soybean Grown under Induced Drought Stress. Indian Journal of Agricultural Research. 54(5): 592-598. doi: 10.18805/IJARe.A-441.

ABSTRACT

Seed priming has potential to improve seedling development and plant growth under environmental stress. In this study, seeds of soybean cultivar LS678 and TGx1835-10E were pretreated with an optimum level of benzyladenine (4.87 mgL-1) before sowing into pots containing pasteurised mixture of vermiculite and sand. Plants were grown up to V3 stage before exposure to moderate and severe drought stress. According to the results, germination was rapid in hydroprimed seeds than BA primed seeds, which took longer to emerge. However, growth, yield and biomass of BA primed plants were increased (number of branches per plant- 7.32, flowering-87.6%, 100 seed weight- 22.6 g, overall biomass fraction- >40.5%) compared to plants developed from hydroprimed seeds (number of branches- 3.61, seed weight- 19.2 g, biomass- <12%) under similar growth conditions. This study indicated that, hormonal seed priming with BA reasonably enhanced soybean growth, particularly root biomass, flowering and fruiting. These effects further suggest that BA may play a significant role in improving drought tolerance in soybean.

REFERENCES

  1. Atteya, A.K.G., AI Taweel, S.K., Genaidy, E.A.E. and Zahran, H.A. (2018). Effect of gibberellic acid and zinc sulphate on vegetative, flowering, seed yield and chemical consistent of jojoba plant (Simmondsia chinensis). Indian Journal of Agricultural Research. 52: 542-547. DOI: 10.18805/    IJARee.A-349.
  2. Boschi, C.L., Palazuelos, M. and Gandolfo, E. (2016). Effect of immersion in solution with 6-benzylaminopurine on the germination and growth of seeds of Ginkgo biloba L. Phyton International Journal of Experimental Botany. 83: 341-    346. URL: www.revistaphyton.fund_romularaggio.org.ar. 
  3. Dong, S., Jiang, Y., Dong, Y., Wang, L., Wang, W., Ma, Z., Yan, C., Ma, C. and Liu, L. (2019). A study on soybean responses to drought stress and rehydration. Saudi Journal of Biological Sciences. 26: 2006-2017.
  4. El-Ghamery, A.A and Mousa, M.A. (2017). Investigation on the effect of benzyladenine on the germination, radicle growth and meristematic cells of Nigella sativa L. and Allium cepa L. Annals of Agricultural Sciences. 62: 11-21. DOI:10.1016/    j.aoas.2016.11.002. 
  5. Gao, S.R., Zhao, Z.G., Hou, J.L., Wang, W.Q., Song, Y., Yan, B.B. and Jin, Y.Q. (2015). Effects of plant growth regulator uniconazole on plant morphology and biomass allocation of Salvia miltiorrhiza. Chinese Marine Materia Medica. 40: 1925-1929. URL: www.ncbi.nlm.nih.gov/pubmed/    26390649.
  6. Jewell, M.C., Campbell, B.C. and Godwin, I.D. (2010). Transgenic plants for abiotic stress resistance. In: Transgenic Crop Plants: Utilization and Biosafety, Kole C., Michler C.H., Abbott A.G., Hall T.C. Springer-Verlag Heidelberg, Berlin, Germany. Vol 2: pp. 67-132.
  7. Kron, A.P., Souza, G.M. and Ribeiro, R.V. (2018). Water deficiency at different developmental stages of Glycine max can improve drought tolerance. Bragantia. 67: 43-49. DOI: 10.1590/S0006-87052008000100005.
  8. Kurnet, K.J., Vorster, B.J., Fenta, B.A., Kibido, T., Dionisio, G. and Foyer, C.H. (2016). Drought stress responses in soybean roots and nodules. Frontier in Plant Science. 7: 1-7. DOI: 10.3389/fpls.2016.01015.
  9. Liu Z., Goto, Y. and Nishiyama, I. (2000). Effects of benzylaminopurine on shoot and root development and growth of rice (cv. North Rose) grown hydroponically with different nitrogen forms. Plant Production Science. 3: 349-353. DOI:10. 1626/pps.3.349.
  10. Mangena, P. (2018). Water stress: Morphological and anatomical changes in soybean (Glycine max L.) plants. In: Plant, Abiotic Stress and Responses to Climate Change, by Violeta Andjelkovic. IntechOpen, London, UK. pp. 9-31. DOI:10.5772/intechopen.72899. 
  11. Mangena, P. and Mokwala, P.W. (2019). The influence of seed viability on the germination and in vitro multiple shoot regeneration in soybean (Glycine max L.). Agriculture. 9: 1-12. DOI: 10.3390/agriculture9020035. 
  12. Mangena, P., Mokwala, P.W. and Nikolova, R.V. (2015). In vitro multiple shoot induction in soybean. International Journal of Agriculture and Biology. 17: 838-842. DOI: 17957/IJAB/    14.0006.
  13. Mansour, F.A., El-Shahaby, O.A., Mostafa, H.A.M., Gaber, M.A. and Ramadan, A.A. (2014). Effect of benzyladenine on growth, pigments and productivity of soybean plant. Egyptian Journal of Physiological Science. 18: 245-364. URL: www.ijfas.com.
  14. Mazrou, M.M., Afify, M.M., El-Kholy, S.A. and Morsy, G.A. (1994). Physiological studies on Ocimum basillicum plant. I.-    influence of kinetin application on the growth and essential oil content. Monofiya Journal of Agricultural Research. 19: 421-434.
  15. McCourt, P. and Creelman, R. (2008). The ABA receptors- we report you decide. Current Opinion in Plant Biology. 11: 474-478. DOI: 10.1016/j.pbi.2008.06.014. 
  16. Ortiz, R. (1998). Critical role of plant biotechnology for the genetic improvement of food crops: Perspective for the next millennium. Electronic Journal of Biotechnology. 1: 1-8. URL: www.agsci.kvl.dk/breed.
  17. Pooter, F.A., Niklas, K.J., Reich, P.B., Oleksyn, J., Pieter, P. and Mommer, L. (2011). Biomass allocation to leaves, stems and roots: Meta-analyses of inter specific variation and environmental control. New Physiologists. 193: 30-50. DOI: 10.1111/j.1469-8137.2011.03952.x.
  18. Rangwala, T., Bafna, A., Vyas, N. and Gupta, R. (2018). Role of soluble silica in altering oxidative stress in soybean crop. Indian Journal of Agricultural Research. 52: 9-15. DOI: 10.18805/IJRe.A-4882.
  19. Raveendra, Y.C., Shirol, A.M. and Kulkami, B.S. (2014). Influence of gibberellic acid on growth, yield and quality of daisy (Aster amellus L.) genotypes. Indian Journal of Agricultural Research. 48: 319-323. DOI: 10.5958/0976-058X.204. 00667.2
  20. Saadawy, F. and Abdel-Moniem, A.M. (2015). Effect of some factors on growth and development of Euphorbia milii var. longifolia. Middle East Journal of Agricultural Research. 4: 613-628. URL: www.curresweb.com/mejar/mejar/2015/613-628.pdf.
  21. Sale, F.A. (2016). Effect of different growth hormones on seed germination and seedling growth of African locust bean (Parkia biglobosa (Jacq) Benth). International Journal of Forestry and Horticulture. 2: 14-20. DOI:10.20431/2454-    9487.0202002.
  22. Sardoei, A.S. (2014). Plant growth regulators effects on the growth and photosynthetic pigments on three indoor ornamental plants. European Journal of Experimental Biology. 4: 311-    318. URL: www.pelagiaresearchlibrary.com.
  23. Suo, H.C., Wang, L.W., Ashraf, K.H., Lui, J.H., Hu, J.G, Li, Z.J., Zhang, X.L., Xie, L. and Zheng, J.R. (2017). Plant growth regulators in seed coating agent affect seed germination and seedling growth of sweet corn. Applied Ecology and Environmental Research. 15: 829-839. DOI: 10.15666/aeer/    1504_829839. 
  24. Verma, H., Badole, P.S. and Nawange, D.D. (2016). Effect of gibberellic acids and naphthalene acetic acids on growth and yield of okra [Abelmoschus esculentus (L.) Moench] var. Arka Anamika. Indian Journal of Agricultural Research. 50: 495-497. DOI: 10.18805/ijare.v5015.3754.
  25. Wei, Y., Jin, J., Jiang, S., Ning, S. and Liu, L. (2018). Quantitative response of soybean development and yield to drought stress during different growth stages in the Huaibei Plain, China. Agriculture. 8: 1-16. DOI: 10.3390/agronomy8070097. 
  26. Zhao, S.L., Suo, J.Z. and Chen, L.Z. (1992). Effects of plant hormone on germination of Bupleurum falcatum L. seeds. Chinese Marine Materia Medica. 17: 715-762. URL: www.ncbi.nlm. nih.gov/pubmed/1304750.
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Indian Journal of Agricultural Research
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    Research Article

    volume 54 issue 5 (october 2020) : 592-598,   Doi: 10.18805/IJARe.A-441

    Effect of Hormonal Seed Priming on Germination, Growth, Yield and Biomass Allocation in Soybean Grown under Induced Drought Stress

    P
    P. Mangena
    1Department of Biodiversity, School of Molecular and Life Sciences, Faculty of Science and Agriculture, University of Limpopo, Private Bag X1106, Sovenga 0727, South Africa.
    Cite article:- Mangena P. (2020). Effect of Hormonal Seed Priming on Germination, Growth, Yield and Biomass Allocation in Soybean Grown under Induced Drought Stress. Indian Journal of Agricultural Research. 54(5): 592-598. doi: 10.18805/IJARe.A-441.

    ABSTRACT

    Seed priming has potential to improve seedling development and plant growth under environmental stress. In this study, seeds of soybean cultivar LS678 and TGx1835-10E were pretreated with an optimum level of benzyladenine (4.87 mgL-1) before sowing into pots containing pasteurised mixture of vermiculite and sand. Plants were grown up to V3 stage before exposure to moderate and severe drought stress. According to the results, germination was rapid in hydroprimed seeds than BA primed seeds, which took longer to emerge. However, growth, yield and biomass of BA primed plants were increased (number of branches per plant- 7.32, flowering-87.6%, 100 seed weight- 22.6 g, overall biomass fraction- >40.5%) compared to plants developed from hydroprimed seeds (number of branches- 3.61, seed weight- 19.2 g, biomass- <12%) under similar growth conditions. This study indicated that, hormonal seed priming with BA reasonably enhanced soybean growth, particularly root biomass, flowering and fruiting. These effects further suggest that BA may play a significant role in improving drought tolerance in soybean.

    REFERENCES

    1. Atteya, A.K.G., AI Taweel, S.K., Genaidy, E.A.E. and Zahran, H.A. (2018). Effect of gibberellic acid and zinc sulphate on vegetative, flowering, seed yield and chemical consistent of jojoba plant (Simmondsia chinensis). Indian Journal of Agricultural Research. 52: 542-547. DOI: 10.18805/    IJARee.A-349.
    2. Boschi, C.L., Palazuelos, M. and Gandolfo, E. (2016). Effect of immersion in solution with 6-benzylaminopurine on the germination and growth of seeds of Ginkgo biloba L. Phyton International Journal of Experimental Botany. 83: 341-    346. URL: www.revistaphyton.fund_romularaggio.org.ar. 
    3. Dong, S., Jiang, Y., Dong, Y., Wang, L., Wang, W., Ma, Z., Yan, C., Ma, C. and Liu, L. (2019). A study on soybean responses to drought stress and rehydration. Saudi Journal of Biological Sciences. 26: 2006-2017.
    4. El-Ghamery, A.A and Mousa, M.A. (2017). Investigation on the effect of benzyladenine on the germination, radicle growth and meristematic cells of Nigella sativa L. and Allium cepa L. Annals of Agricultural Sciences. 62: 11-21. DOI:10.1016/    j.aoas.2016.11.002. 
    5. Gao, S.R., Zhao, Z.G., Hou, J.L., Wang, W.Q., Song, Y., Yan, B.B. and Jin, Y.Q. (2015). Effects of plant growth regulator uniconazole on plant morphology and biomass allocation of Salvia miltiorrhiza. Chinese Marine Materia Medica. 40: 1925-1929. URL: www.ncbi.nlm.nih.gov/pubmed/    26390649.
    6. Jewell, M.C., Campbell, B.C. and Godwin, I.D. (2010). Transgenic plants for abiotic stress resistance. In: Transgenic Crop Plants: Utilization and Biosafety, Kole C., Michler C.H., Abbott A.G., Hall T.C. Springer-Verlag Heidelberg, Berlin, Germany. Vol 2: pp. 67-132.
    7. Kron, A.P., Souza, G.M. and Ribeiro, R.V. (2018). Water deficiency at different developmental stages of Glycine max can improve drought tolerance. Bragantia. 67: 43-49. DOI: 10.1590/S0006-87052008000100005.
    8. Kurnet, K.J., Vorster, B.J., Fenta, B.A., Kibido, T., Dionisio, G. and Foyer, C.H. (2016). Drought stress responses in soybean roots and nodules. Frontier in Plant Science. 7: 1-7. DOI: 10.3389/fpls.2016.01015.
    9. Liu Z., Goto, Y. and Nishiyama, I. (2000). Effects of benzylaminopurine on shoot and root development and growth of rice (cv. North Rose) grown hydroponically with different nitrogen forms. Plant Production Science. 3: 349-353. DOI:10. 1626/pps.3.349.
    10. Mangena, P. (2018). Water stress: Morphological and anatomical changes in soybean (Glycine max L.) plants. In: Plant, Abiotic Stress and Responses to Climate Change, by Violeta Andjelkovic. IntechOpen, London, UK. pp. 9-31. DOI:10.5772/intechopen.72899. 
    11. Mangena, P. and Mokwala, P.W. (2019). The influence of seed viability on the germination and in vitro multiple shoot regeneration in soybean (Glycine max L.). Agriculture. 9: 1-12. DOI: 10.3390/agriculture9020035. 
    12. Mangena, P., Mokwala, P.W. and Nikolova, R.V. (2015). In vitro multiple shoot induction in soybean. International Journal of Agriculture and Biology. 17: 838-842. DOI: 17957/IJAB/    14.0006.
    13. Mansour, F.A., El-Shahaby, O.A., Mostafa, H.A.M., Gaber, M.A. and Ramadan, A.A. (2014). Effect of benzyladenine on growth, pigments and productivity of soybean plant. Egyptian Journal of Physiological Science. 18: 245-364. URL: www.ijfas.com.
    14. Mazrou, M.M., Afify, M.M., El-Kholy, S.A. and Morsy, G.A. (1994). Physiological studies on Ocimum basillicum plant. I.-    influence of kinetin application on the growth and essential oil content. Monofiya Journal of Agricultural Research. 19: 421-434.
    15. McCourt, P. and Creelman, R. (2008). The ABA receptors- we report you decide. Current Opinion in Plant Biology. 11: 474-478. DOI: 10.1016/j.pbi.2008.06.014. 
    16. Ortiz, R. (1998). Critical role of plant biotechnology for the genetic improvement of food crops: Perspective for the next millennium. Electronic Journal of Biotechnology. 1: 1-8. URL: www.agsci.kvl.dk/breed.
    17. Pooter, F.A., Niklas, K.J., Reich, P.B., Oleksyn, J., Pieter, P. and Mommer, L. (2011). Biomass allocation to leaves, stems and roots: Meta-analyses of inter specific variation and environmental control. New Physiologists. 193: 30-50. DOI: 10.1111/j.1469-8137.2011.03952.x.
    18. Rangwala, T., Bafna, A., Vyas, N. and Gupta, R. (2018). Role of soluble silica in altering oxidative stress in soybean crop. Indian Journal of Agricultural Research. 52: 9-15. DOI: 10.18805/IJRe.A-4882.
    19. Raveendra, Y.C., Shirol, A.M. and Kulkami, B.S. (2014). Influence of gibberellic acid on growth, yield and quality of daisy (Aster amellus L.) genotypes. Indian Journal of Agricultural Research. 48: 319-323. DOI: 10.5958/0976-058X.204. 00667.2
    20. Saadawy, F. and Abdel-Moniem, A.M. (2015). Effect of some factors on growth and development of Euphorbia milii var. longifolia. Middle East Journal of Agricultural Research. 4: 613-628. URL: www.curresweb.com/mejar/mejar/2015/613-628.pdf.
    21. Sale, F.A. (2016). Effect of different growth hormones on seed germination and seedling growth of African locust bean (Parkia biglobosa (Jacq) Benth). International Journal of Forestry and Horticulture. 2: 14-20. DOI:10.20431/2454-    9487.0202002.
    22. Sardoei, A.S. (2014). Plant growth regulators effects on the growth and photosynthetic pigments on three indoor ornamental plants. European Journal of Experimental Biology. 4: 311-    318. URL: www.pelagiaresearchlibrary.com.
    23. Suo, H.C., Wang, L.W., Ashraf, K.H., Lui, J.H., Hu, J.G, Li, Z.J., Zhang, X.L., Xie, L. and Zheng, J.R. (2017). Plant growth regulators in seed coating agent affect seed germination and seedling growth of sweet corn. Applied Ecology and Environmental Research. 15: 829-839. DOI: 10.15666/aeer/    1504_829839. 
    24. Verma, H., Badole, P.S. and Nawange, D.D. (2016). Effect of gibberellic acids and naphthalene acetic acids on growth and yield of okra [Abelmoschus esculentus (L.) Moench] var. Arka Anamika. Indian Journal of Agricultural Research. 50: 495-497. DOI: 10.18805/ijare.v5015.3754.
    25. Wei, Y., Jin, J., Jiang, S., Ning, S. and Liu, L. (2018). Quantitative response of soybean development and yield to drought stress during different growth stages in the Huaibei Plain, China. Agriculture. 8: 1-16. DOI: 10.3390/agronomy8070097. 
    26. Zhao, S.L., Suo, J.Z. and Chen, L.Z. (1992). Effects of plant hormone on germination of Bupleurum falcatum L. seeds. Chinese Marine Materia Medica. 17: 715-762. URL: www.ncbi.nlm. nih.gov/pubmed/1304750.
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