Indian Journal Of Agricultural Research

Efficacy of organic solvents for medium term storage of oil palm (Elaeis guineensis Jacq.) pollen

DOI: 10.18805/ijare.v49i6.6678    | Article Id: A-4271 | Page : 516-521
Citation :- Efficacy of organic solvents for medium term storage of oil palm(Elaeis guineensis Jacq.) pollen .Indian Journal Of Agricultural Research.2015.(49):516-521

K. Sunil Kumar*, R.K. Mathur and D. S. Sparjanbabu

sunilk.icar@gmail.com
Address :

ICAR-Indian Institute of Oil Palm Research, West Godavari District, 
Pedavegi–534 450, Andhra Pradesh, India.

Submitted Date : 27-09-2014
Accepted Date : 5-08-2015
First Online:

Abstract

The oil palm is the richest source of vegetable oil in the world. The hybrid nature of crop necessitates artificial hybridization between proven parent palms which in turn require the pollen from male parent to be collected and stored without loss of viability for sufficiently long period. Reports   suggested that pollen grains of various taxa could be successfully preserved in different organic solvents. In the present study, attempt was made to   evaluate the suitability  of organic solvents to store the oil palm pollen at 0 to -5oC temperature. Among the five solvents used, there was significant variation for viability and germination percentages. Viability was the maximum for pollen stored in Diethyl ether (90.45 %) as well as in n-Hexane (88.41%) after 200 days of storage.  Viability of pollen stored in Chloroform and Acetone were moderate and in case of Methyl alcohol, complete loss of viability was observed. With respect to pollen germination in vitro, similar trend was observed. The trend was same at different intervals/period of storage. This was confirmed through in vivo pollination and fruits set studies. Thus, present method indicated that it is feasible to store oil palm pollen in non polar solvents beyond 200 days without considerable loss of viability or germination.

Keywords

Elaeis Oil palm Organic solvents Pollen Storage.

References

  1. Corely , RHV and Tinker, P.B. (2003). The Oil Palm. Blackwell publishing , pp 562.
  2. Franchi G., G., Bellani, L., Nepi, M., Pacini, E. (1996) Types of carbohydrate reserves in pollen: localization, systematic distribution and ecophysiological significance. Flora. 191:143-159. 
  3. Ganeshan, S. (1998). Pollen storage in tropical fruits, P. 120-125. In: R.K. Arora and R. V. Ramanatha (eds). Tropical Fruits in Asia, Diversity, Maintenance, Conservation and Use. IPGRI.
  4. Grout, B.,(1995) Genetic preservation of plant cells in vitro. 1995, pp169.
  5. Helpson-Harrison J., Helpson-Harrison Y. and Shivanna,K.R. (1984). The evaluation of pollen quality and a further appraisal of the flourochromatic (FCR) test procedure. Theor. Appl. Genet.67: 367-375
  6. Heslop-Harrison,J. and Heslop-Harrison,Y.(1992). Intracellular motility, the actin cytoskeleton and germinability in the pollen of wheat (Triticum aestivum L.).Sex Plant Reprod.5:247-255.
  7. Iwaanami,Y.(1972a).Viability of pollen grains in organic solvents. Botanique 3:61-68.
  8. Iwanami, Y. (1972b).Retaining the viability of Camellia japonica pollen in various organic solvents. Plant Cell Physiol.13:1139-1141.
  9. Iwanami,Y. and Nakamura, N.(1972).Storage in an organic solvent as a means for preserving viability of pollen grains. Stain Technol. 47:137-139.
  10. Jain, A. and Shivanna, K.R.(1988a). Storage of pollen grains in organic solvents: effects of organic solvents on leaching of phospholipids and its relationship to pollen viability. Ann.Bot.61:325-330.
  11. Jain, A. and Shivanna, K.R.(1988b).Storage of pollen grains in organic solvents: effects of solvents on pollen viability and membrane integrity. J. Plant Physiol.132:499-501.
  12. Jain, A. and Shivanna, K.R.(1990).Membrane state and pollen viability during storage in organic solvents. In: Proceedings of the International Congress Plant Physiology, New Delhi Soc.Plant Physiol. & Biochem.,eds.S.K.Sinha, P.V.Sane, S.C.Bhargava and P.K. Agarwal,pp.1341-1349.
  13. Jain, A. and Shivanna,K.R.(1989).Loss of viability during storage is associated with changes in membrane phospholipid. Phytochem 28: 999-1002.
  14. Jangam, A.K. and Thaliat, P. ( 2014). WASP - Web Agri Stat Package 2.0 ICAR Research Complex for Goa, Ela, Old Goa, Goa. 403 402. India. http://www.icargoa.res.in/wasp/crd1.php
  15. Khan, S.A. and Perveen, A. (2006). Genetic capacity, viability and maintenance of stored pollen of Vigna mungo L. Int.J. Biol. Biotech., 3:779-781 
  16. Khan, S.A. and Perveen, A. (2010). In vitro pollen germination capacity of Citrullus lanatus L. (Cucurbitaceae). Pak. J. Bot., 42:681-684
  17. Liu, W.,Ma,L. & Cao, Z.(1985).Studies on the viability of pollen of 11 species stored in some organic solvents. Acta Hort. Sinica 12:69-71.
  18. Lopez-Diaz, S. and Garay B.R. (2008). Simple methods for in vitro pollen germination and pollen preservation of selected species of the genus Agave. e- Gnosis (online),Vol. 6, Art.2
  19. Mishra,R. and Shivanna, K.R.(1982).Efficacy of organic solvents for storing pollen grains of some leguminous taxa. Euphytica 31:991-995
  20. Myint, K.A. , Rafii, M.Y., SheikhAbdulla. S.A., Win, N.M., Mohd Din, A. and Latif, M.A. (2012). Determination of the optimum pollen germination medium for different fruit forms of oil palm (Elaeis guineensis). J. Animal Plant Sciences 14:1855-1865
  21. Panse, V.G. and Sukhatme,P.V. , (1967).Statistical Methods for Agricultural Research Workers, ICAR, New Delhi.
  22. Perveen, A. (2007) Pollen germination capacity, viability and maintenance of Pisum sativum L. (Papilionaceae). Middle East J. Sci. Res. 2 :79-81
  23. Rao ,V. Soh, A.C., Corely, R.H.V., Lee CH, Rajanaidu, N., Tan Y.P., Chin C.W. Lim K.C., Tan S.T., Lee T.P. and Ngui M. (1983). A critical re-examination of the method of bunch quality analysis in oil palm breeding. Palm oil Res. Inst. Malaysia, Occ. paper, 9:1-28
  24. Shivanna, K.R. and Heslop-Harrison, J. (1981). Membrane state and pollen viability. Ann. Bot.47:759-770
  25. Stanley R. G. and Linskens, H. F. (1974). Pollen: biology, biochemistry, and management. Springer-Verlag, New York, New York, USA 
  26. Sunilkumar, K., Mathur. R.K. and Sparjanbabu, D.S. (2011). Efficacy of dyes and media on pollen viability and germinability in oil palm ((Elaeis guineensis Jacq.), Intl. J. Oil palm Research 8:9-12
  27. Tandon, R., Manohara, T.N., Nijalingappa, B.H.M. and Shivanna, K.R. (2001). Pollination and pollen-pistil interaction in oil palm, Elaeis guineensis. Annals of Botany 87: 831-838. 
  28. Wilson, J.R., M.J.Fisher, E.D.Schulze,G.R.Dolby, and M.M.Lud low. (1979).Comparison between pressure-volume and dew point hygrometry techniques for determining the water relations characteristics of grass and legume leaves. Oecologia 41:77-88.

Global Footprints