Indian Journal of Agricultural Research

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Research Article

Indian Journal of Agricultural Research, volume 54 issue 5 (october 2020) : 629-634

Genetic Diversity in Common Bean (Phaseolus vulgaris L.) Germplasm to Establish a Breeding Program in the Mountainous Region of Jammu and Kashmir

Asad Hussain Shah, Ishtiaq Khaliq, Ghosia Bashir, Sammya Jannat
1Tun Abdul Razak Research Centre, Brickendonbury, Hertford United Kingdom, SG13 8NL.
Cite article:- Shah Hussain Asad, Khaliq Ishtiaq, Bashir Ghosia, Jannat Sammya (2020). Genetic Diversity in Common Bean (Phaseolus vulgaris L.) Germplasm to Establish a Breeding Program in the Mountainous Region of Jammu and Kashmir. Indian Journal of Agricultural Research. 54(5): 629-634. doi: 10.18805/IJARe.A-421.

ABSTRACT

Common bean (Phaseolus vulgaris L.) is native to Middle America and Andean mountains of South America. Due to its nutritious value, it is one of the most important legume crop of the world. Evaluation of genetic diversity of a crop is extremely important for the establishment of a breeding program and selection of varieties for cultivation. We evaluated the genetic diversity and phylogenetic relationship of 40 P. vulgaris ecotypes from CIAT (International Center for Tropical Agriculture) germplasm using seed protein profiles produced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), to screen the germplasm to be used in a breeding program. A total of 21 bands scored 14 were found polymorphic. A distance matrix was generated from the similarity matrix based on polymorphic bands. The UPGMA tree was established through cluster analysis performed on the distance matrix. Six major clusters were formed on the basis of SDS_PAGE analysis with one ecotype (E13-G16832) being the most diverse. This analysis provided the basis for selection of suitable ecotypes to be used in the establishment of a breeding program.

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