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volume 48 issue 1 (february 2014) : 67-71,   Doi: 10.5958/j.0976-058X.48.1.012

SCREENING OF BARLEY GERMPLASM FOR LEAF BLIGHT (BIPOLARIS SOROKINIANA) RESISTANCE

N
Neha Jain1
R
Rekha Malik*
R
R. Selvakumar1
R
Rajendera Kumar
V
Veena Pande1
R
R.P.S. Verma
1Directorate of Wheat Research (DWR), Karnal- 132 001, India
Cite article:- Jain1 Neha, Malik* Rekha, Selvakumar1 R., Kumar Rajendera, Pande1 Veena, Verma R.P.S. (2025). SCREENING OF BARLEY GERMPLASM FOR LEAF BLIGHT (BIPOLARIS SOROKINIANA) RESISTANCE. Indian Journal of Agricultural Research. 48(1): 67-71. doi: 10.5958/j.0976-058X.48.1.012.

ABSTRACT

Spot blotch or leaf spot, caused by Bipolaris sorokiniana (earlier Helminthosporium sativum) is the most common form of leaf blights of barley which appears in warmer and humid weather. The genetic studies for leaf blight in barley have concluded complex inheritance of leaf blight resistance at adult plant stage. Current Indian barley varieties are mostly susceptible to leaf blight under high input conditions and attempts have been made to introduce resistance for this fungal disease in Indian barley.  In the present study, a set of 85 barley germplasm accessions under epiphytotic conditions were screened for leaf blight disease occurrence. Of these lines, 68 genotypes were found resistant, 7 were moderately resistant, 7 were moderately susceptible and 3 were found susceptible. The genotypes grouped as resistant were able to keep the disease level on the plant at very low level may be useful in developing mapping populations for QTL studies. The molecular markers reported for QTL regions of leaf blight resistance were used to screen these lines to validate their applicability in molecular studies for leaf blight in Indian conditions. Three molecular markers (Ebmac 705, Bmag 606 and Bmag 225) located on 3H genomic region of barley were found associated with leaf blight resistance during molecular screening. This suggests that the genomic region at 3H chromosome is significantly involved in inheritance of leaf blight resistance in Indian barley. This study may prove useful in selection of plant material and marker assisted selection for incorporating leaf blight resistance in Indian barley.

REFERENCES

  1. Anonymous (2009). Progress Report of the All India Coordinated Wheat and Barley Improvement Project. 2008-09. 6: pp. 224. Barley Network. DWR Karnal, India.
  2. Bilgic, H., Steffenson, B. J. and Hayes, P. M. (2005). Comprehensive genetic analysis reveals differential expression of seedling and adult plant resistance to spot blotch in populations of barley. Theor. Appl. Genet., 111:1238- 1250.
  3. Bilgic, H., Steffenson, B. J., and Hayes, P. M. (2006). Molecular mapping of loci conferring resistance to different pathotypes of the spot blotch pathogen in barley. Phytopathology, 96:699-708.
  4. Bowill, J., Lehmensiek, A., Sutherland, M. W., Platz, G. J., Usher, T., Franckowiak, J. and Mace, E. (2010). Mapping spot blotch resistance genes in four barley populations. Mol. Breed., 26:653-666(14).
  5. Chand, R., Sen, D., Prasad, K.D., Singh, A.K., Bashyal, B.M., Prasad, L.C. and Joshi, A.K. (2008). Screening for disease resistance in barley cultivars against Bipolaris sorokiniana using callus culture method. Indian. J. Exp. Bot., 46:249-253.
  6. Fetch, T. G. Jr. and Steffenson, B. J. (1999). Rating scales for assessing infection responses of barley infected with Cochliobolus sativus. Plant Dis., 83:213–217.
  7. Joshi, A. K. and Chand, R. (2002). Variation and inheritance of leaf angle and its association with spot blotch (Bipolaris sorokiniana) severity in wheat (Triticum aestivum). Euphytica, 124:283-291.
  8. Joshi, A. K., Ferrara, O., Crossa, J., Singh, G., Sharma, R., Chand, R. and Parsad, R. (2007c). Combining superior agronomic perfor- mance and terminal heat tolerance with resistance to spot blotch (Bipolaris sorokiniana) in the warm humid Gangetic plains of south Asia. Field Crop Res., 103:53-61.
  9. Joshi, A. K., Ortiz-Ferrara, G., Crossa, J., Singh G., Alvarado, G., Bhatta, M. R. , Duveiller, E., Sharma, R. C., Pandit, D. B., Siddique, A. B. (2007b). Associations of environments in South Asia based on spot blotch disease of wheat caused by Cochliobolus sativus. Crop Sci., 47:1071-1084.
  10. Kumar, J., Schafer, P., Huckelhoven, R., Langen, G., Baltruschat, H., Stein, E., Nagarajan, S., Kogel, K. H. (2002). Pathogen Profile: Bipolaris sorokiniana, a cereal pathogen of global concern. Cytological and molecular approaches towards better control. Mol. Pl. Pathol., 3:185-195.
  11. Kumar, J., Sharma, A. K., Kumar, R. and Nagarajan, S. (1999). A new technique to develop artificial epiphytotics of foliar blights in wheat. Indian J. Agric. Sci., 69(1):49-52.
  12. Kumar, J., Singh, G. and Nagarajan, S. (1998). A field scale for leaf blight recording. Wheat and Barley Newsl., 4(2): pp. 3.
  13. Kutcher, H. R., Bailey, K. L., Rossnagel, B. G., and Legge, W. G. (1994). Heritability of common root rot and spot blotch resistance in barley. Can. J. Pl. Pathol., 16:287-294.
  14. Nagarajan, S. and Kumar, J. (1998). Foliar blights of wheat in India. In: Helminthosporium blights of wheat, spot blotch and tan spot. (Edited by Duveiller E., Dubin H. J., Reeves. J. and Mc Nab, A). Proc. Intl. Workshop held at CIMMYT, Mexico pp. 52-58.
  15. Roy, K. J., Smith, K. P., Muehlbauer, G. J., Chao, S., Close, T. J., Steffenson, B. J. (2010). Association mapping of spot blotch resistance in wild barley. Mol. Breed., 26:243–256
  16. Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A. and Allard, R. W. (1984). Ribosomal DNA spacer length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. 81:8014- 8018. Proc. Natl. Acad. Sci., USA.
  17. Singh, D. P., Singh, R., Verma, R. P. S., Sarkar B. and Shoran, J. (2005). Status of resistance to yellow rust and leaf blight in popular varieties and sources of combined resistance to both diseases in barley. Pl. Dis. Res. 20: 87.
  18. Verma, R. P. S., Singh, D. P. and Sarkar, B. (2002). Resistance to net blotch (Helminthosporium teres Sacc.) and leaf spot (Bipolaris sorokiniana (Sacc.) Shoemaker) in barley. Indian. J. Plant Genet. Resources, 15(1):17-18.
  19. Steffenson, B. J., Hayes, P. M. and Kleinhofs. (1996). Genetics of seeding and adult plant resistance to net blotch (Pyrenophora teres f. teres) and spot blotch (Cochliobolus sativus) in barley. Theor. Appl. Genet., 92:552-558.
  20. Tyagi, K., Nandan, R., Prasad, L., Joshi, A. K. and Chand, R. (2008). Inheritance and identification of molecular markers associated with leaf blight (Cochliobolus sativus L.) resistance through microsatellites analysis in barley. Genet. Mol. Biol., 31 (3):734-742.
  21. Wilcoxson, R. D, Rasmusson, D. C and Miles, M. R. (1990). Development of barley resistant to leaf blight and genetics of resistance. Plant Dis., 74:207-210.
  22. Zhou, H., Jannick, J. L. and Stefensson, B. J. (2009). Association mapping of Adult Plant Spot Blotch Resistance in Barley Breeding Germplasm from the USA. pp. 263. XVII PAG Conference.
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Indian Journal of Agricultural Research
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    volume 48 issue 1 (february 2014) : 67-71,   Doi: 10.5958/j.0976-058X.48.1.012

    SCREENING OF BARLEY GERMPLASM FOR LEAF BLIGHT (BIPOLARIS SOROKINIANA) RESISTANCE

    N
    Neha Jain1
    R
    Rekha Malik*
    R
    R. Selvakumar1
    R
    Rajendera Kumar
    V
    Veena Pande1
    R
    R.P.S. Verma
    1Directorate of Wheat Research (DWR), Karnal- 132 001, India
    Cite article:- Jain1 Neha, Malik* Rekha, Selvakumar1 R., Kumar Rajendera, Pande1 Veena, Verma R.P.S. (2025). SCREENING OF BARLEY GERMPLASM FOR LEAF BLIGHT (BIPOLARIS SOROKINIANA) RESISTANCE. Indian Journal of Agricultural Research. 48(1): 67-71. doi: 10.5958/j.0976-058X.48.1.012.

    ABSTRACT

    Spot blotch or leaf spot, caused by Bipolaris sorokiniana (earlier Helminthosporium sativum) is the most common form of leaf blights of barley which appears in warmer and humid weather. The genetic studies for leaf blight in barley have concluded complex inheritance of leaf blight resistance at adult plant stage. Current Indian barley varieties are mostly susceptible to leaf blight under high input conditions and attempts have been made to introduce resistance for this fungal disease in Indian barley.  In the present study, a set of 85 barley germplasm accessions under epiphytotic conditions were screened for leaf blight disease occurrence. Of these lines, 68 genotypes were found resistant, 7 were moderately resistant, 7 were moderately susceptible and 3 were found susceptible. The genotypes grouped as resistant were able to keep the disease level on the plant at very low level may be useful in developing mapping populations for QTL studies. The molecular markers reported for QTL regions of leaf blight resistance were used to screen these lines to validate their applicability in molecular studies for leaf blight in Indian conditions. Three molecular markers (Ebmac 705, Bmag 606 and Bmag 225) located on 3H genomic region of barley were found associated with leaf blight resistance during molecular screening. This suggests that the genomic region at 3H chromosome is significantly involved in inheritance of leaf blight resistance in Indian barley. This study may prove useful in selection of plant material and marker assisted selection for incorporating leaf blight resistance in Indian barley.

    REFERENCES

    1. Anonymous (2009). Progress Report of the All India Coordinated Wheat and Barley Improvement Project. 2008-09. 6: pp. 224. Barley Network. DWR Karnal, India.
    2. Bilgic, H., Steffenson, B. J. and Hayes, P. M. (2005). Comprehensive genetic analysis reveals differential expression of seedling and adult plant resistance to spot blotch in populations of barley. Theor. Appl. Genet., 111:1238- 1250.
    3. Bilgic, H., Steffenson, B. J., and Hayes, P. M. (2006). Molecular mapping of loci conferring resistance to different pathotypes of the spot blotch pathogen in barley. Phytopathology, 96:699-708.
    4. Bowill, J., Lehmensiek, A., Sutherland, M. W., Platz, G. J., Usher, T., Franckowiak, J. and Mace, E. (2010). Mapping spot blotch resistance genes in four barley populations. Mol. Breed., 26:653-666(14).
    5. Chand, R., Sen, D., Prasad, K.D., Singh, A.K., Bashyal, B.M., Prasad, L.C. and Joshi, A.K. (2008). Screening for disease resistance in barley cultivars against Bipolaris sorokiniana using callus culture method. Indian. J. Exp. Bot., 46:249-253.
    6. Fetch, T. G. Jr. and Steffenson, B. J. (1999). Rating scales for assessing infection responses of barley infected with Cochliobolus sativus. Plant Dis., 83:213–217.
    7. Joshi, A. K. and Chand, R. (2002). Variation and inheritance of leaf angle and its association with spot blotch (Bipolaris sorokiniana) severity in wheat (Triticum aestivum). Euphytica, 124:283-291.
    8. Joshi, A. K., Ferrara, O., Crossa, J., Singh, G., Sharma, R., Chand, R. and Parsad, R. (2007c). Combining superior agronomic perfor- mance and terminal heat tolerance with resistance to spot blotch (Bipolaris sorokiniana) in the warm humid Gangetic plains of south Asia. Field Crop Res., 103:53-61.
    9. Joshi, A. K., Ortiz-Ferrara, G., Crossa, J., Singh G., Alvarado, G., Bhatta, M. R. , Duveiller, E., Sharma, R. C., Pandit, D. B., Siddique, A. B. (2007b). Associations of environments in South Asia based on spot blotch disease of wheat caused by Cochliobolus sativus. Crop Sci., 47:1071-1084.
    10. Kumar, J., Schafer, P., Huckelhoven, R., Langen, G., Baltruschat, H., Stein, E., Nagarajan, S., Kogel, K. H. (2002). Pathogen Profile: Bipolaris sorokiniana, a cereal pathogen of global concern. Cytological and molecular approaches towards better control. Mol. Pl. Pathol., 3:185-195.
    11. Kumar, J., Sharma, A. K., Kumar, R. and Nagarajan, S. (1999). A new technique to develop artificial epiphytotics of foliar blights in wheat. Indian J. Agric. Sci., 69(1):49-52.
    12. Kumar, J., Singh, G. and Nagarajan, S. (1998). A field scale for leaf blight recording. Wheat and Barley Newsl., 4(2): pp. 3.
    13. Kutcher, H. R., Bailey, K. L., Rossnagel, B. G., and Legge, W. G. (1994). Heritability of common root rot and spot blotch resistance in barley. Can. J. Pl. Pathol., 16:287-294.
    14. Nagarajan, S. and Kumar, J. (1998). Foliar blights of wheat in India. In: Helminthosporium blights of wheat, spot blotch and tan spot. (Edited by Duveiller E., Dubin H. J., Reeves. J. and Mc Nab, A). Proc. Intl. Workshop held at CIMMYT, Mexico pp. 52-58.
    15. Roy, K. J., Smith, K. P., Muehlbauer, G. J., Chao, S., Close, T. J., Steffenson, B. J. (2010). Association mapping of spot blotch resistance in wild barley. Mol. Breed., 26:243–256
    16. Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A. and Allard, R. W. (1984). Ribosomal DNA spacer length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. 81:8014- 8018. Proc. Natl. Acad. Sci., USA.
    17. Singh, D. P., Singh, R., Verma, R. P. S., Sarkar B. and Shoran, J. (2005). Status of resistance to yellow rust and leaf blight in popular varieties and sources of combined resistance to both diseases in barley. Pl. Dis. Res. 20: 87.
    18. Verma, R. P. S., Singh, D. P. and Sarkar, B. (2002). Resistance to net blotch (Helminthosporium teres Sacc.) and leaf spot (Bipolaris sorokiniana (Sacc.) Shoemaker) in barley. Indian. J. Plant Genet. Resources, 15(1):17-18.
    19. Steffenson, B. J., Hayes, P. M. and Kleinhofs. (1996). Genetics of seeding and adult plant resistance to net blotch (Pyrenophora teres f. teres) and spot blotch (Cochliobolus sativus) in barley. Theor. Appl. Genet., 92:552-558.
    20. Tyagi, K., Nandan, R., Prasad, L., Joshi, A. K. and Chand, R. (2008). Inheritance and identification of molecular markers associated with leaf blight (Cochliobolus sativus L.) resistance through microsatellites analysis in barley. Genet. Mol. Biol., 31 (3):734-742.
    21. Wilcoxson, R. D, Rasmusson, D. C and Miles, M. R. (1990). Development of barley resistant to leaf blight and genetics of resistance. Plant Dis., 74:207-210.
    22. Zhou, H., Jannick, J. L. and Stefensson, B. J. (2009). Association mapping of Adult Plant Spot Blotch Resistance in Barley Breeding Germplasm from the USA. pp. 263. XVII PAG Conference.
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